PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; stem eudicotyledons; Proteales; Nelumbonaceae; Nelumbo

Family

WRKY

Protein Properties

Length: 685aa MW: 74870.9 Da PI: 6.6774

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
NNU_024102-RA	genome	CAS	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	97.1	1.2e-30	409	467	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 Dg++WrKYGqK+ kg+++pr+YYrCt+a gCpv+k+v+r+aed +++++tYeg+Hnh+ NNU_024102-RA 409 TDGCQWRKYGQKMAKGNPCPRAYYRCTMAaGCPVRKQVQRCAEDRSILITTYEGNHNHP 467 7*************************99**************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	1.2E-33	393	469	IPR003657	WRKY domain
SuperFamily	SSF118290	8.76E-28	401	469	IPR003657	WRKY domain
PROSITE profile	PS50811	29.792	403	469	IPR003657	WRKY domain
SMART	SM00774	3.2E-37	408	468	IPR003657	WRKY domain
Pfam	PF03106	6.7E-27	410	467	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0010200	Biological Process	response to chitin
GO:0016036	Biological Process	cellular response to phosphate starvation
GO:0045892	Biological Process	negative regulation of transcription, DNA-templated
GO:0080169	Biological Process	cellular response to boron-containing substance deprivation
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding
GO:0044212	Molecular Function	transcription regulatory region DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 685 aa Download sequence Send to blast
MELVGIQHRR GGFLKRPEYS TGESCSGRVE LRFTSRCNVS VGREEKRRER IAAGLRCSLV 60 SDDREREEEN SGRKRWNEKG GGGLTIDSSE IGFFVTKPTA IKSFLTTNHP KWKHDPMDFP 120 VNLNCRDDQD SPVPSDEKRV DEMDFFSDKN RLKDFDAKAS IIDVKKEDSH GGVAPRLDLA 180 VNTGLHLLTA NTGSDQSTVD DGISPNADDK RAKSELAALQ TELDRMNEEN QRLRAMLSQV 240 TNNYSALQMH LVTLMQQQNR KAESAQDQEM LDGKLEEKKH EAGEGLIVPR QFMDLGPAAT 300 AETDEPSQSS SEGRSRDHSG SPHNNAEVVS KEYRQQKNSS NNEIVLFDQD KSDFGEDRGI 360 GREESPDQGW VANKIPKLNP SKNVDQTTEA TMRKARVSVR ARSEAPMITD GCQWRKYGQK 420 MAKGNPCPRA YYRCTMAAGC PVRKQVQRCA EDRSILITTY EGNHNHPLPP AAMAMASTTS 480 AAASMLLSGS MSSTDGLLNS NFLARTLLPC SSSMATISAS APFPTVTLDL THTPNPLQFQ 540 RPPSQFHVPF PNQAQSFPSQ PAPPLTQVFG QALYNQTKFS GLQLSQDVEP SQFVHQSPAP 600 APHQAQQPSL ADTVSAATAA ITADPNFTAA LAAAITSIIG GARPTNNSSS NDNNNNSSNN 660 NGNTTTTSNS NGNNNKISNS TFPGN

Sequence ? help Back to Top

Protein Sequence Length: 685 aa Download sequence Send to blast

MELVGIQHRR GGFLKRPEYS TGESCSGRVE LRFTSRCNVS VGREEKRRER IAAGLRCSLV  60
SDDREREEEN SGRKRWNEKG GGGLTIDSSE IGFFVTKPTA IKSFLTTNHP KWKHDPMDFP  120
VNLNCRDDQD SPVPSDEKRV DEMDFFSDKN RLKDFDAKAS IIDVKKEDSH GGVAPRLDLA  180
VNTGLHLLTA NTGSDQSTVD DGISPNADDK RAKSELAALQ TELDRMNEEN QRLRAMLSQV  240
TNNYSALQMH LVTLMQQQNR KAESAQDQEM LDGKLEEKKH EAGEGLIVPR QFMDLGPAAT  300
AETDEPSQSS SEGRSRDHSG SPHNNAEVVS KEYRQQKNSS NNEIVLFDQD KSDFGEDRGI  360
GREESPDQGW VANKIPKLNP SKNVDQTTEA TMRKARVSVR ARSEAPMITD GCQWRKYGQK  420
MAKGNPCPRA YYRCTMAAGC PVRKQVQRCA EDRSILITTY EGNHNHPLPP AAMAMASTTS  480
AAASMLLSGS MSSTDGLLNS NFLARTLLPC SSSMATISAS APFPTVTLDL THTPNPLQFQ  540
RPPSQFHVPF PNQAQSFPSQ PAPPLTQVFG QALYNQTKFS GLQLSQDVEP SQFVHQSPAP  600
APHQAQQPSL ADTVSAATAA ITADPNFTAA LAAAITSIIG GARPTNNSSS NDNNNNSSNN  660
NGNTTTTSNS NGNNNKISNS TFPGN

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
2ayd_A	4e-22	395	471	1	76	WRKY transcription factor 1
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor involved in the control of processes related to senescence and pathogen defense (PubMed:12000796). Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (PubMed:12000796). Activates the transcription of the SIRK gene and represses its own expression and that of the WRKY42 genes (PubMed:12000796). Modulates phosphate homeostasis and Pi translocation by regulating PHO1 expression (PubMed:25733771). {ECO:0000269\|PubMed:12000796, ECO:0000269\|PubMed:25733771}.

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00209	DAP	Transfer from AT1G62300	Download

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By salicylic acid, ethylene, jasmonic acid, pathogens, wounding and strongly during leaf senescence. {ECO:0000269\|PubMed:11449049, ECO:0000269\|PubMed:11722756}.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_010244474.1	0.0	PREDICTED: probable WRKY transcription factor 31
Swissprot	Q9C519	1e-147	WRKY6_ARATH; WRKY transcription factor 6
TrEMBL	A0A1U7Z9S9	0.0	A0A1U7Z9S9_NELNU; probable WRKY transcription factor 31
STRING	XP_010244474.1	0.0	(Nelumbo nucifera)

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT1G62300.1	1e-122	WRKY family protein

Publications ? help Back to Top
Zhou F, et al. High humidity suppresses ssi4-mediated cell death and disease resistance upstream of MAP kinase activation, H2O2 production and defense gene expression. Plant J., 2004. 39(6): p. 920-32 [PMID:15341634] Ramírez V,López A,Mauch-Mani B,Gil MJ,Vera P An extracellular subtilase switch for immune priming in Arabidopsis. PLoS Pathog., 2013. 9(6): p. e1003445 [PMID:23818851] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Singh V,Roy S,Singh D,Nandi AK Arabidopsis flowering locus D influences systemic-acquired-resistance- induced expression and histone modifications of WRKY genes. J. Biosci., 2014. 39(1): p. 119-26 [PMID:24499796] Ali MA,Wieczorek K,Kreil DP,Bohlmann H The beet cyst nematode Heterodera schachtii modulates the expression of WRKY transcription factors in syncytia to favour its development in Arabidopsis roots. PLoS ONE, 2014. 9(7): p. e102360 [PMID:25033038] Chai J,Liu J,Zhou J,Xing D Mitogen-activated protein kinase 6 regulates NPR1 gene expression and activation during leaf senescence induced by salicylic acid. J. Exp. Bot., 2014. 65(22): p. 6513-28 [PMID:25210078] Vermeirssen V,De Clercq I,Van Parys T,Van Breusegem F,Van de Peer Y Arabidopsis ensemble reverse-engineered gene regulatory network discloses interconnected transcription factors in oxidative stress. Plant Cell, 2014. 26(12): p. 4656-79 [PMID:25549671] Xiao D, et al. SENESCENCE-SUPPRESSED PROTEIN PHOSPHATASE Directly Interacts with the Cytoplasmic Domain of SENESCENCE-ASSOCIATED RECEPTOR-LIKE KINASE and Negatively Regulates Leaf Senescence in Arabidopsis. Plant Physiol., 2015. 169(2): p. 1275-91 [PMID:26304848] Bakshi M, et al. WRKY6 restricts Piriformospora indica-stimulated and phosphate-induced root development in Arabidopsis. BMC Plant Biol., 2015. 15: p. 305 [PMID:26718529] Huang Y,Feng CZ,Ye Q,Wu WH,Chen YF Arabidopsis WRKY6 Transcription Factor Acts as a Positive Regulator of Abscisic Acid Signaling during Seed Germination and Early Seedling Development. PLoS Genet., 2016. 12(2): p. e1005833 [PMID:26829043] Ramírez V,López A,Mauch-Mani B,Gil MJ,Vera P Correction: An Extracellular Subtilase Switch for Immune Priming in Arabidopsis. PLoS Pathog., 2016. 12(11): p. e1006003 [PMID:27806116] Li H, et al. BZR1 Positively Regulates Freezing Tolerance via CBF-Dependent and CBF-Independent Pathways in Arabidopsis. Mol Plant, 2017. 10(4): p. 545-559 [PMID:28089951] Stetter MG,Benz M,Ludewig U Increased root hair density by loss of WRKY6 in Arabidopsis thaliana. PeerJ, 2017. 5: p. e2891 [PMID:28149680] Zhou X, et al. StMYB44 negatively regulates phosphate transport by suppressing expression of PHOSPHATE1 in potato. J. Exp. Bot., 2017. 68(5): p. 1265-1281 [PMID:28338870] Bakshi M, et al. Piriformospora indica Reprograms Gene Expression in Arabidopsis Phosphate Metabolism Mutants But Does Not Compensate for Phosphate Limitation. Front Microbiol, 2017. 8: p. 1262 [PMID:28747898] Zhang Y, et al. DELLA proteins negatively regulate dark-induced senescence and chlorophyll degradation in Arabidopsis through interaction with the transcription factor WRKY6. Plant Cell Rep., 2018. 37(7): p. 981-992 [PMID:29574486]