PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT1G62300.1
Common NameATWRKY6, F19K23.22, WRKY6
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family WRKY
Protein Properties Length: 553aa    MW: 60593.1 Da    PI: 6.2553
Description WRKY family protein
Gene Model
Gene Model ID Type Source Coding Sequence
AT1G62300.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
         WRKY   2 dDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 
                  +Dg++WrKYGqK+ kg+++pr+YYrCt+a gCpv+k+v+r+aed +++++tYeg+Hnh+
                  7***************************99****************************7 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
Gene3DG3DSA: domain
SuperFamilySSF1182904.58E-28304372IPR003657WRKY domain
PROSITE profilePS5081129.792306372IPR003657WRKY domain
SMARTSM007742.4E-37311371IPR003657WRKY domain
PfamPF031064.7E-27313370IPR003657WRKY domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0009873Biological Processethylene-activated signaling pathway
GO:0010200Biological Processresponse to chitin
GO:0016036Biological Processcellular response to phosphate starvation
GO:0045892Biological Processnegative regulation of transcription, DNA-templated
GO:0080169Biological Processcellular response to boron-containing substance deprivation
GO:0005634Cellular Componentnucleus
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005515Molecular Functionprotein binding
GO:0043565Molecular Functionsequence-specific DNA binding
GO:0044212Molecular Functiontranscription regulatory region DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000025anatomyroot tip
PO:0000084anatomyplant sperm cell
PO:0000230anatomyinflorescence meristem
PO:0000256anatomyroot hair cell
PO:0000293anatomyguard cell
PO:0009009anatomyplant embryo
PO:0009025anatomyvascular leaf
PO:0025022anatomycollective leaf structure
PO:0001185developmental stageplant embryo globular stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 553 aa     Download sequence    Send to blast
3D Structure ? help Back to Top
PDB ID Evalue Query Start Query End Hit Start Hit End Description
2ayd_A3e-22298374176WRKY transcription factor 1
Search in ModeBase
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.252600.0leaf| root| vegetative tissue
Expression -- Microarray ? help Back to Top
Source ID E-value
Expression AtlasAT1G62300-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Roots, leaves, shoots, flowers and siliques.
Functional Description ? help Back to Top
Source Description
TAIREncodes a transcription factor WRKY6. Regulates Phosphate1 (Pho1) expression in response to low phosphate (Pi) stress.
UniProtTranscription factor involved in the control of processes related to senescence and pathogen defense (PubMed:12000796). Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (PubMed:12000796). Activates the transcription of the SIRK gene and represses its own expression and that of the WRKY42 genes (PubMed:12000796). Modulates phosphate homeostasis and Pi translocation by regulating PHO1 expression (PubMed:25733771). {ECO:0000269|PubMed:12000796, ECO:0000269|PubMed:25733771}.
Function -- GeneRIF ? help Back to Top
  1. Our results demonstrate that WRKY6 and WRKY42 are involved in Arabidopsis responses to low Pi stress by regulation of PHO1 expression.
    [PMID: 19934380]
  2. Results established that WRKY6 is a low-B-induced transcription factor gene that is essential for normal root growth under low-B conditions.
    [PMID: 20059736]
  3. WRKY6 is an arsenate-responsive transcription factor that mediates arsenate/phosphate transporter gene expression and restricts arsenate-induced transposon activation.
    [PMID: 23922208]
  4. WRKY40 and WRKY6 act downstream of the green leaf volatile E-2-hexenal.WRKY40 and WRKY6 mediate inhibition of root elongation by E-2-hexenal.
    [PMID: 26243404]
  5. WRKY6 restricts Piriformospora indica infection and phosphate-induced root development.
    [PMID: 26718529]
  6. Arabidopsis WRKY6 transcription factor played important roles in abscissic acid signaling by directly down-regulating RAV1 expression.
    [PMID: 26829043]
  7. WRKY6 positively regulates dark-induced senescence and chlorophyll degradation.RGA physically interacts with WRKY6.
    [PMID: 29574486]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
Motif logo
Cis-element ? help Back to Top
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: By salicylic acid, ethylene, jasmonic acid, pathogens, wounding and strongly during leaf senescence. {ECO:0000269|PubMed:11449049, ECO:0000269|PubMed:11722756}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
Regulation -- ATRM (Manually Curated Upstream Regulators) ? help Back to Top
Source Upstream Regulator (A: Activate/R: Repress)
ATRM AT1G62300 (R), AT2G03340 (A)
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT1G17420(A), AT1G45145(A), AT1G62300(R), AT2G14610(A), AT3G23430(R), AT4G04450(R)
Interaction ? help Back to Top
Source Intact With
IntActSearch Q9C519
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT1G62300
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAF3317120.0AF331712.1 Arabidopsis thaliana transcription factor WRKY6 (WRKY6) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_564792.10.0WRKY family transcription factor
SwissprotQ9C5190.0WRKY6_ARATH; WRKY transcription factor 6
TrEMBLC0SV110.0C0SV11_ARATH; Uncharacterized protein At1g62300 (Fragment)
STRINGAT1G62300.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Representative plantOGRP1417875
Publications ? help Back to Top
  1. Eulgem T,Rushton PJ,Robatzek S,Somssich IE
    The WRKY superfamily of plant transcription factors.
    Trends Plant Sci., 2000. 5(5): p. 199-206
  2. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
  3. Yu D,Chen C,Chen Z
    Evidence for an important role of WRKY DNA binding proteins in the regulation of NPR1 gene expression.
    Plant Cell, 2001. 13(7): p. 1527-40
  4. Robatzek S,Somssich IE
    A new member of the Arabidopsis WRKY transcription factor family, AtWRKY6, is associated with both senescence- and defence-related processes.
    Plant J., 2001. 28(2): p. 123-33
  5. Robatzek S,Somssich IE
    Targets of AtWRKY6 regulation during plant senescence and pathogen defense.
    Genes Dev., 2002. 16(9): p. 1139-49
  6. Whitham SA, et al.
    Diverse RNA viruses elicit the expression of common sets of genes in susceptible Arabidopsis thaliana plants.
    Plant J., 2003. 33(2): p. 271-83
  7. Laloi C,Mestres-Ortega D,Marco Y,Meyer Y,Reichheld JP
    The Arabidopsis cytosolic thioredoxin h5 gene induction by oxidative stress and its W-box-mediated response to pathogen elicitor.
    Plant Physiol., 2004. 134(3): p. 1006-16
  8. Zhou F, et al.
    High humidity suppresses ssi4-mediated cell death and disease resistance upstream of MAP kinase activation, H2O2 production and defense gene expression.
    Plant J., 2004. 39(6): p. 920-32
  9. Zou X,Seemann JR,Neuman D,Shen QJ
    A WRKY gene from creosote bush encodes an activator of the abscisic acid signaling pathway.
    J. Biol. Chem., 2004. 279(53): p. 55770-9
  10. Libault M,Wan J,Czechowski T,Udvardi M,Stacey G
    Identification of 118 Arabidopsis transcription factor and 30 ubiquitin-ligase genes responding to chitin, a plant-defense elicitor.
    Mol. Plant Microbe Interact., 2007. 20(8): p. 900-11
  11. Ciolkowski I,Wanke D,Birkenbihl RP,Somssich IE
    Studies on DNA-binding selectivity of WRKY transcription factors lend structural clues into WRKY-domain function.
    Plant Mol. Biol., 2008. 68(1-2): p. 81-92
  12. Skibbe M,Qu N,Galis I,Baldwin IT
    Induced plant defenses in the natural environment: Nicotiana attenuata WRKY3 and WRKY6 coordinate responses to herbivory.
    Plant Cell, 2008. 20(7): p. 1984-2000
  13. Kim CY,Bove J,Assmann SM
    Overexpression of wound-responsive RNA-binding proteins induces leaf senescence and hypersensitive-like cell death.
    New Phytol., 2008. 180(1): p. 57-70
  14. Chang IF, et al.
    Proteomic profiling of tandem affinity purified 14-3-3 protein complexes in Arabidopsis thaliana.
    Proteomics, 2009. 9(11): p. 2967-85
  15. Chen YF, et al.
    The WRKY6 transcription factor modulates PHOSPHATE1 expression in response to low Pi stress in Arabidopsis.
    Plant Cell, 2009. 21(11): p. 3554-66
  16. Kasajima I,Ide Y,Yokota Hirai M,Fujiwara T
    WRKY6 is involved in the response to boron deficiency in Arabidopsis thaliana.
    Physiol Plant, 2010. 139(1): p. 80-92
  17. Shin R,Jez JM,Basra A,Zhang B,Schachtman DP
    14-3-3 proteins fine-tune plant nutrient metabolism.
    FEBS Lett., 2011. 585(1): p. 143-7
  18. Zhou X,Jiang Y,Yu D
    WRKY22 transcription factor mediates dark-induced leaf senescence in Arabidopsis.
    Mol. Cells, 2011. 31(4): p. 303-13
  19. Causier B,Ashworth M,Guo W,Davies B
    The TOPLESS interactome: a framework for gene repression in Arabidopsis.
    Plant Physiol., 2012. 158(1): p. 423-38
  20. Luna E,Bruce TJ,Roberts MR,Flors V,Ton J
    Next-generation systemic acquired resistance.
    Plant Physiol., 2012. 158(2): p. 844-53
  21. Vogelmann K, et al.
    Early senescence and cell death in Arabidopsis saul1 mutants involves the PAD4-dependent salicylic acid pathway.
    Plant Physiol., 2012. 159(4): p. 1477-87
  22. Efroni I, et al.
    Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses.
    Dev. Cell, 2013. 24(4): p. 438-45
  23. Mackov
    Enhanced drought and heat stress tolerance of tobacco plants with ectopically enhanced cytokinin oxidase/dehydrogenase gene expression.
    J. Exp. Bot., 2013. 64(10): p. 2805-15
  24. Ramírez V,López A,Mauch-Mani B,Gil MJ,Vera P
    An extracellular subtilase switch for immune priming in Arabidopsis.
    PLoS Pathog., 2013. 9(6): p. e1003445
  25. Castrillo G, et al.
    WRKY6 transcription factor restricts arsenate uptake and transposon activation in Arabidopsis.
    Plant Cell, 2013. 25(8): p. 2944-57
  26. Ding Y, et al.
    Four distinct types of dehydration stress memory genes in Arabidopsis thaliana.
    BMC Plant Biol., 2013. 13: p. 229
  27. Singh V,Roy S,Singh D,Nandi AK
    Arabidopsis flowering locus D influences systemic-acquired-resistance- induced expression and histone modifications of WRKY genes.
    J. Biosci., 2014. 39(1): p. 119-26
  28. Ali MA,Wieczorek K,Kreil DP,Bohlmann H
    The beet cyst nematode Heterodera schachtii modulates the expression of WRKY transcription factors in syncytia to favour its development in Arabidopsis roots.
    PLoS ONE, 2014. 9(7): p. e102360
  29. Chai J,Liu J,Zhou J,Xing D
    Mitogen-activated protein kinase 6 regulates NPR1 gene expression and activation during leaf senescence induced by salicylic acid.
    J. Exp. Bot., 2014. 65(22): p. 6513-28
  30. Vermeirssen V,De Clercq I,Van Parys T,Van Breusegem F,Van de Peer Y
    Arabidopsis ensemble reverse-engineered gene regulatory network discloses interconnected transcription factors in oxidative stress.
    Plant Cell, 2014. 26(12): p. 4656-79
  31. Su T, et al.
    WRKY42 modulates phosphate homeostasis through regulating phosphate translocation and acquisition in Arabidopsis.
    Plant Physiol., 2015. 167(4): p. 1579-91
  32. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
  33. Mirabella R, et al.
    WRKY40 and WRKY6 act downstream of the green leaf volatile E-2-hexenal in Arabidopsis.
    Plant J., 2015. 83(6): p. 1082-96
  34. Xiao D, et al.
    SENESCENCE-SUPPRESSED PROTEIN PHOSPHATASE Directly Interacts with the Cytoplasmic Domain of SENESCENCE-ASSOCIATED RECEPTOR-LIKE KINASE and Negatively Regulates Leaf Senescence in Arabidopsis.
    Plant Physiol., 2015. 169(2): p. 1275-91
  35. Bakshi M, et al.
    WRKY6 restricts Piriformospora indica-stimulated and phosphate-induced root development in Arabidopsis.
    BMC Plant Biol., 2015. 15: p. 305
  36. Huang Y,Feng CZ,Ye Q,Wu WH,Chen YF
    Arabidopsis WRKY6 Transcription Factor Acts as a Positive Regulator of Abscisic Acid Signaling during Seed Germination and Early Seedling Development.
    PLoS Genet., 2016. 12(2): p. e1005833
  37. Ramírez V,López A,Mauch-Mani B,Gil MJ,Vera P
    Correction: An Extracellular Subtilase Switch for Immune Priming in Arabidopsis.
    PLoS Pathog., 2016. 12(11): p. e1006003
  38. Li H, et al.
    BZR1 Positively Regulates Freezing Tolerance via CBF-Dependent and CBF-Independent Pathways in Arabidopsis.
    Mol Plant, 2017. 10(4): p. 545-559
  39. Stetter MG,Benz M,Ludewig U
    Increased root hair density by loss of WRKY6 in Arabidopsis thaliana.
    PeerJ, 2017. 5: p. e2891
  40. Zhou X, et al.
    StMYB44 negatively regulates phosphate transport by suppressing expression of PHOSPHATE1 in potato.
    J. Exp. Bot., 2017. 68(5): p. 1265-1281
  41. Bakshi M, et al.
    Piriformospora indica Reprograms Gene Expression in Arabidopsis Phosphate Metabolism Mutants But Does Not Compensate for Phosphate Limitation.
    Front Microbiol, 2017. 8: p. 1262
  42. Zhang Y, et al.
    DELLA proteins negatively regulate dark-induced senescence and chlorophyll degradation in Arabidopsis through interaction with the transcription factor WRKY6.
    Plant Cell Rep., 2018. 37(7): p. 981-992