PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

SMil_00021799-RA_Salv

Common Name

WRKY46

Organism

Salvia miltiorrhiza

Taxonomic ID

226208

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Lamiales; Lamiaceae; Nepetoideae; Mentheae; Salvia

Family

WRKY

Protein Properties

Length: 306aa MW: 33427 Da PI: 6.9005

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
SMil_00021799-RA_Salv	genome	NDCTCM	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	97	1.2e-30	142	200	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 +D ++WrKYGqK++kgs++pr+YYrC+++ gC+++k+ver+++dp ++++tY+ eHnh+ SMil_00021799-RA_Salv 142 SDVWSWRKYGQKPIKGSPYPRGYYRCSTSkGCMARKQVERNRSDPGMFIVTYTAEHNHP 200 79**********************9988**************************8 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	7.7E-31	130	202	IPR003657	WRKY domain
SuperFamily	SSF118290	7.45E-27	136	202	IPR003657	WRKY domain
PROSITE profile	PS50811	35.416	136	202	IPR003657	WRKY domain
SMART	SM00774	5.4E-37	141	201	IPR003657	WRKY domain
Pfam	PF03106	5.7E-26	143	200	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0010150	Biological Process	leaf senescence
GO:0010200	Biological Process	response to chitin
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 306 aa Download sequence Send to blast
MEDDWDLHAV VRGCAAATTN TRSLQALYGH QQACSSFSAS SFQDLLEPRR NGVVREGLID 60 LYNYKPLFPN SEVPFSLPVS TISALVGSQD PPPQTQLRPK HHSFSVSNAS KTSASHSPTP 120 RSKRRKTHTK KVCHVAAENL SSDVWSWRKY GQKPIKGSPY PRGYYRCSTS KGCMARKQVE 180 RNRSDPGMFI VTYTAEHNHP MPTHRNSLAG STRNKPADPT GTSFSPEVSP ATSLSPPAEK 240 LESSREEELA EDDDDGVFSV SEMALDDDFF AGLEDFTAAD PDCASGHLQF PWLSTSATTT 300 TTAAGG

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
5w3x_B	2e-21	129	203	5	80	Disease resistance protein RRS1
5w3x_D	2e-21	129	203	5	80	Disease resistance protein RRS1
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Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	122	127	KRRKTH

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor involved in the expression of defense genes in innate immune response of plants. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Activates WRKY 29, SIRK and its own promoters. {ECO:0000269\|PubMed:11875555}.

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00422	DAP	Transfer from AT4G01250	Download

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Induced after flagellin treatment. {ECO:0000269\|PubMed:11875555}.

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	KM823169	0.0	KM823169.1 Salvia miltiorrhiza WRKY protein (WRKY46) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_011092990.1	1e-121	WRKY transcription factor 22
Swissprot	O04609	1e-85	WRK22_ARATH; WRKY transcription factor 22
TrEMBL	A0A0D5Y9B4	0.0	A0A0D5Y9B4_SALMI; WRKY protein
STRING	Migut.B01797.1.p	1e-99	(Erythranthe guttata)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Asterids	OGEA2591	24	57

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT4G01250.1	2e-84	WRKY family protein

Publications ? help Back to Top
Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Coego A, et al. The TRANSPLANTA collection of Arabidopsis lines: a resource for functional analysis of transcription factors based on their conditional overexpression. Plant J., 2014. 77(6): p. 944-53 [PMID:24456507] Gkizi D, et al. The Innate Immune Signaling System as a Regulator of Disease Resistance and Induced Systemic Resistance Activity Against Verticillium dahliae. Mol. Plant Microbe Interact., 2016. 29(4): p. 313-23 [PMID:26780421] Kloth KJ, et al. AtWRKY22 promotes susceptibility to aphids and modulates salicylic acid and jasmonic acid signalling. J. Exp. Bot., 2016. 67(11): p. 3383-96 [PMID:27107291] Camborde L, et al. Detection of nucleic acid-protein interactions in plant leaves using fluorescence lifetime imaging microscopy. Nat Protoc, 2017. 12(9): p. 1933-1950 [PMID:28837131] Wang S, et al. Bacillus cereus AR156 Activates Defense Responses to Pseudomonas syringae pv. tomato in Arabidopsis thaliana Similarly to flg22. Mol. Plant Microbe Interact., 2018. 31(3): p. 311-322 [PMID:29090631]