PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT4G01250.1

Common Name

A_IG002N01.6, AtWRKY22, F2N1.6, WRKY22

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

WRKY

Protein Properties

Length: 298aa MW: 32255.6 Da PI: 7.566

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT4G01250.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	98.1	5.6e-31	128	186	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 +D ++WrKYGqK++kgs++pr+YYrC+++ gC ++k+ver+++dpk++++tY+ eHnh+ AT4G01250.1 128 SDVWAWRKYGQKPIKGSPYPRGYYRCSTSkGCLARKQVERNRSDPKMFIVTYTAEHNHP 186 69**********************9988**************************8 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	1.8E-30	117	188	IPR003657	WRKY domain
PROSITE profile	PS50811	36.77	122	188	IPR003657	WRKY domain
SuperFamily	SSF118290	3.92E-26	124	188	IPR003657	WRKY domain
SMART	SM00774	8.4E-36	127	187	IPR003657	WRKY domain
Pfam	PF03106	7.0E-26	129	186	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0006952	Biological Process	defense response
GO:0010150	Biological Process	leaf senescence
GO:0010200	Biological Process	response to chitin
GO:0005634	Cellular Component	nucleus
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000013	anatomy	cauline leaf
PO:0000037	anatomy	shoot apex
PO:0000230	anatomy	inflorescence meristem
PO:0000293	anatomy	guard cell
PO:0008019	anatomy	leaf lamina base
PO:0009005	anatomy	root
PO:0009006	anatomy	shoot system
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009025	anatomy	vascular leaf
PO:0009029	anatomy	stamen
PO:0009030	anatomy	carpel
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0009052	anatomy	flower pedicel
PO:0020030	anatomy	cotyledon
PO:0020038	anatomy	petiole
PO:0020100	anatomy	hypocotyl
PO:0020137	anatomy	leaf apex
PO:0025022	anatomy	collective leaf structure
PO:0001054	developmental stage	vascular leaf senescent stage
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0001081	developmental stage	mature plant embryo stage
PO:0001185	developmental stage	plant embryo globular stage
PO:0004507	developmental stage	plant embryo bilateral stage
PO:0007064	developmental stage	LP.12 twelve leaves visible stage
PO:0007095	developmental stage	LP.08 eight leaves visible stage
PO:0007098	developmental stage	LP.02 two leaves visible stage
PO:0007103	developmental stage	LP.10 ten leaves visible stage
PO:0007115	developmental stage	LP.04 four leaves visible stage
PO:0007123	developmental stage	LP.06 six leaves visible stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 298 aa Download sequence Send to blast
MADDWDLHAV VRGCSAVSSS ATTTVYSPGV SSHTNPIFTV GRQSNAVSFG EIRDLYTPFT 60 QESVVSSFSC INYPEEPRKP QNQKRPLSLS ASSGSVTSKP SGSNTSRSKR RKIQHKKVCH 120 VAAEALNSDV WAWRKYGQKP IKGSPYPRGY YRCSTSKGCL ARKQVERNRS DPKMFIVTYT 180 AEHNHPAPTH RNSLAGSTRQ KPSDQQTSKS PTTTIATYSS SPVTSADEFV LPVEDHLAVG 240 DLDGEEDLLS LSDTVVSDDF FDGLEEFAAG DSFSGNSAPA SFDLSWVVNS AATTTGGI

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
5w3x_B	1e-20	129	189	18	80	Disease resistance protein RRS1
5w3x_D	1e-20	129	189	18	80	Disease resistance protein RRS1
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Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.3803	0.0	bud\| root

Expression -- Microarray ? help Back to Top
Source	ID	E-value
Genevisible	255568_at	0.0
Expression Atlas	AT4G01250	-
AtGenExpress	AT4G01250	-
ATTED-II	AT4G01250	-

Functional Description ? help Back to Top
Source	Description
TAIR	member of WRKY Transcription Factor; Group II-e
UniProt	Transcription factor involved in the expression of defense genes in innate immune response of plants. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Activates WRKY 29, SIRK and its own promoters. {ECO:0000269\|PubMed:11875555}.

Function -- GeneRIF ? help Back to Top
AtWRKY22 participates in the dark-induced senescence signal transduction pathway [PMID: 21359674] Data indicate that WRKY22 T-DNA insertion mutants wrky22-1 and wrky22-2 had lower disease resistance and lower induction of innate immunity markers, such as FLG22-INDUCED RECEPTOR-LIKE KINASE1 (FRK1) and WRKY53, after submergence. [PMID: 23897923] WRKY22 modulates the interplay between the salicylic acid (SA) and jasmonic acid (JA) pathways in response to a wide range of biotic and abiotic stimuli. Its induction by aphids and its role in suppressing SA and JA signalling make WRKY22 a potential target for aphids to manipulate host plant defences. [PMID: 27107291]

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00422	DAP	27203113	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT4G01250.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Induced after flagellin treatment. {ECO:0000269\|PubMed:11875555}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source	Target Gene (A: Activate/R: Repress)
ATRM	AT2G19190(A), AT4G23550(A)

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT4G01250

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AF442392	0.0	AF442392.1 Arabidopsis thaliana WRKY transcription factor 22 (WRKY22) mRNA, complete cds.
GenBank	AY045909	0.0	AY045909.1 Arabidopsis thaliana putative DNA-binding protein (At4g01250) mRNA, complete cds.
GenBank	AY079404	0.0	AY079404.1 Arabidopsis thaliana putative DNA-binding protein (At4g01250) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_192034.1	0.0	WRKY family transcription factor
Swissprot	O04609	0.0	WRK22_ARATH; WRKY transcription factor 22
TrEMBL	A0A178V251	0.0	A0A178V251_ARATH; WRKY22
STRING	AT4G01250.1	0.0	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM7810	27	41
Representative plant	OGRP14	17	875

Link Out ? help Back to Top
Phytozome	AT4G01250.1
Entrez Gene	827896
iHOP	AT4G01250
wikigenes	AT4G01250

Publications ? help Back to Top
Eulgem T,Rushton PJ,Robatzek S,Somssich IE The WRKY superfamily of plant transcription factors. Trends Plant Sci., 2000. 5(5): p. 199-206 [PMID:10785665] Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Asai T, et al. MAP kinase signalling cascade in Arabidopsis innate immunity. Nature, 2002. 415(6875): p. 977-83 [PMID:11875555] Folta KM,Pontin MA,Karlin-Neumann G,Bottini R,Spalding EP Genomic and physiological studies of early cryptochrome 1 action demonstrate roles for auxin and gibberellin in the control of hypocotyl growth by blue light. Plant J., 2003. 36(2): p. 203-14 [PMID:14535885] Dal Bosco C, et al. Inactivation of the chloroplast ATP synthase gamma subunit results in high non-photochemical fluorescence quenching and altered nuclear gene expression in Arabidopsis thaliana. J. Biol. Chem., 2004. 279(2): p. 1060-9 [PMID:14576160] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Hoth S, et al. Monitoring genome-wide changes in gene expression in response to endogenous cytokinin reveals targets in Arabidopsis thaliana. FEBS Lett., 2003. 554(3): p. 373-80 [PMID:14623097] Zipfel C, et al. Bacterial disease resistance in Arabidopsis through flagellin perception. Nature, 2004. 428(6984): p. 764-7 [PMID:15085136] Navarro L, et al. The transcriptional innate immune response to flg22. Interplay and overlap with Avr gene-dependent defense responses and bacterial pathogenesis. Plant Physiol., 2004. 135(2): p. 1113-28 [PMID:15181213] Hampton CR, et al. Cesium toxicity in Arabidopsis. Plant Physiol., 2004. 136(3): p. 3824-37 [PMID:15489280] Monte E, et al. The phytochrome-interacting transcription factor, PIF3, acts early, selectively, and positively in light-induced chloroplast development. Proc. Natl. Acad. Sci. U.S.A., 2004. 101(46): p. 16091-8 [PMID:15505214] Miao Y,Laun T,Zimmermann P,Zentgraf U Targets of the WRKY53 transcription factor and its role during leaf senescence in Arabidopsis. Plant Mol. Biol., 2004. 55(6): p. 853-67 [PMID:15604721] Xie Z, et al. Annotations and functional analyses of the rice WRKY gene superfamily reveal positive and negative regulators of abscisic acid signaling in aleurone cells. Plant Physiol., 2005. 137(1): p. 176-89 [PMID:15618416] Lee D,Polisensky DH,Braam J Genome-wide identification of touch- and darkness-regulated Arabidopsis genes: a focus on calmodulin-like and XTH genes. New Phytol., 2005. 165(2): p. 429-44 [PMID:15720654] Lee BH,Henderson DA,Zhu JK The Arabidopsis cold-responsive transcriptome and its regulation by ICE1. Plant Cell, 2005. 17(11): p. 3155-75 [PMID:16214899] Phelps-Durr TL,Thomas J,Vahab P,Timmermans MC Maize rough sheath2 and its Arabidopsis orthologue ASYMMETRIC LEAVES1 interact with HIRA, a predicted histone chaperone, to maintain knox gene silencing and determinacy during organogenesis. Plant Cell, 2005. 17(11): p. 2886-98 [PMID:16243907] Suzuki N, et al. Enhanced tolerance to environmental stress in transgenic plants expressing the transcriptional coactivator multiprotein bridging factor 1c. Plant Physiol., 2005. 139(3): p. 1313-22 [PMID:16244138] Truman W,de Zabala MT,Grant M Type III effectors orchestrate a complex interplay between transcriptional networks to modify basal defence responses during pathogenesis and resistance. Plant J., 2006. 46(1): p. 14-33 [PMID:16553893] Thilmony R,Underwood W,He SY Genome-wide transcriptional analysis of the Arabidopsis thaliana interaction with the plant pathogen Pseudomonas syringae pv. tomato DC3000 and the human pathogen Escherichia coli O157:H7. Plant J., 2006. 46(1): p. 34-53 [PMID:16553894] Tosti N, et al. Gene expression profiles of O3-treated Arabidopsis plants. Plant Cell Environ., 2006. 29(9): p. 1686-702 [PMID:16913859] M The Arabidopsis MAP kinase kinase MKK1 participates in defence responses to the bacterial elicitor flagellin. Plant J., 2006. 48(4): p. 485-98 [PMID:17059410] Serrano M, et al. Chemical interference of pathogen-associated molecular pattern-triggered immune responses in Arabidopsis reveals a potential role for fatty-acid synthase type II complex-derived lipid signals. J. Biol. Chem., 2007. 282(9): p. 6803-11 [PMID:17166839] Libault M,Wan J,Czechowski T,Udvardi M,Stacey G Identification of 118 Arabidopsis transcription factor and 30 ubiquitin-ligase genes responding to chitin, a plant-defense elicitor. Mol. Plant Microbe Interact., 2007. 20(8): p. 900-11 [PMID:17722694] Chawade A,Br Putative cold acclimation pathways in Arabidopsis thaliana identified by a combined analysis of mRNA co-expression patterns, promoter motifs and transcription factors. BMC Genomics, 2007. 8: p. 304 [PMID:17764576] Xu L, et al. Di- and tri- but not monomethylation on histone H3 lysine 36 marks active transcription of genes involved in flowering time regulation and other processes in Arabidopsis thaliana. Mol. Cell. Biol., 2008. 28(4): p. 1348-60 [PMID:18070919] Wan J, et al. A LysM receptor-like kinase plays a critical role in chitin signaling and fungal resistance in Arabidopsis. Plant Cell, 2008. 20(2): p. 471-81 [PMID:18263776] Yamaguchi Y,Huffaker A,Bryan AC,Tax FE,Ryan CA PEPR2 is a second receptor for the Pep1 and Pep2 peptides and contributes to defense responses in Arabidopsis. Plant Cell, 2010. 22(2): p. 508-22 [PMID:20179141] Wan J,Zhang S,Stacey G Activation of a mitogen-activated protein kinase pathway in Arabidopsis by chitin. Mol. Plant Pathol., 2004. 5(2): p. 125-35 [PMID:20565589] Zhou X,Jiang Y,Yu D WRKY22 transcription factor mediates dark-induced leaf senescence in Arabidopsis. Mol. Cells, 2011. 31(4): p. 303-13 [PMID:21359674] Schikora A, et al. N-acyl-homoserine lactone confers resistance toward biotrophic and hemibiotrophic pathogens via altered activation of AtMPK6. Plant Physiol., 2011. 157(3): p. 1407-18 [PMID:21940998] Gonz The conjugated auxin indole-3-acetic acid-aspartic acid promotes plant disease development. Plant Cell, 2012. 24(2): p. 762-77 [PMID:22374398] Göhre V,Jones AM,Sklenář J,Robatzek S,Weber AP Molecular crosstalk between PAMP-triggered immunity and photosynthesis. Mol. Plant Microbe Interact., 2012. 25(8): p. 1083-92 [PMID:22550958] Hsu FC, et al. Submergence confers immunity mediated by the WRKY22 transcription factor in Arabidopsis. Plant Cell, 2013. 25(7): p. 2699-713 [PMID:23897923] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Coego A, et al. The TRANSPLANTA collection of Arabidopsis lines: a resource for functional analysis of transcription factors based on their conditional overexpression. Plant J., 2014. 77(6): p. 944-53 [PMID:24456507] Jin J, et al. An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors. Mol. Biol. Evol., 2015. 32(7): p. 1767-73 [PMID:25750178] Gkizi D, et al. The Innate Immune Signaling System as a Regulator of Disease Resistance and Induced Systemic Resistance Activity Against Verticillium dahliae. Mol. Plant Microbe Interact., 2016. 29(4): p. 313-23 [PMID:26780421] Kloth KJ, et al. AtWRKY22 promotes susceptibility to aphids and modulates salicylic acid and jasmonic acid signalling. J. Exp. Bot., 2016. 67(11): p. 3383-96 [PMID:27107291] Camborde L, et al. Detection of nucleic acid-protein interactions in plant leaves using fluorescence lifetime imaging microscopy. Nat Protoc, 2017. 12(9): p. 1933-1950 [PMID:28837131] Wang S, et al. Bacillus cereus AR156 Activates Defense Responses to Pseudomonas syringae pv. tomato in Arabidopsis thaliana Similarly to flg22. Mol. Plant Microbe Interact., 2018. 31(3): p. 311-322 [PMID:29090631]