PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

SMil_00008294-RA_Salv

Common Name

WRKY39

Organism

Salvia miltiorrhiza

Taxonomic ID

226208

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Lamiales; Lamiaceae; Nepetoideae; Mentheae; Salvia

Family

WRKY

Protein Properties

Length: 385aa MW: 42910 Da PI: 7.5601

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
SMil_00008294-RA_Salv	genome	NDCTCM	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	100.2	1.2e-31	144	200	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhe 59 dDgynWrKYGqK+vkgse+prsYY+Ct+a+Cp+kkkver+ d+ v+ei+Y+g+Hnh SMil_00008294-RA_Salv 144 DDGYNWRKYGQKQVKGSENPRSYYKCTFANCPTKKKVERNL-DGCVTEIVYKGSHNHG 200 8**************************************.*************5 PP
2	WRKY	106	2e-33	265	323	1	59
---SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 1 ldDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhe 59 ldDgy+WrKYGqK+vkg+++prsYY+Cts+gCpv+k+ver+ +d+++v++tYeg+Hnhe SMil_00008294-RA_Salv 265 LDDGYRWRKYGQKVVKGNPNPRSYYKCTSNGCPVRKHVERAFHDNRAVITTYEGKHNHE 323 59********************************************************8 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	2.6E-27	133	202	IPR003657	WRKY domain
PROSITE profile	PS50811	23.215	138	202	IPR003657	WRKY domain
SuperFamily	SSF118290	5.36E-24	139	202	IPR003657	WRKY domain
SMART	SM00774	9.2E-34	143	201	IPR003657	WRKY domain
Pfam	PF03106	4.5E-24	144	199	IPR003657	WRKY domain
Gene3D	G3DSA:2.20.25.80	1.2E-37	250	325	IPR003657	WRKY domain
SuperFamily	SSF118290	1.7E-29	257	325	IPR003657	WRKY domain
PROSITE profile	PS50811	38.067	260	325	IPR003657	WRKY domain
SMART	SM00774	2.0E-38	265	324	IPR003657	WRKY domain
Pfam	PF03106	7.5E-26	266	323	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 385 aa Download sequence Send to blast
LMASSGGSLN PALNSFHYNP TSQYMNSFTD LLTSNKDHYE EKYSFPPLSP PPLSPSFFLD 60 SPALFGSSNA LPSPTTGAFA ALFAKDDGPA FSDFSFHSQT RPSDPSAAPI TSKDCVKRPR 120 EEESWIRKVE EVPRSYVREQ GKSDDGYNWR KYGQKQVKGS ENPRSYYKCT FANCPTKKKV 180 ERNLDGCVTE IVYKGSHNHG KPHANSPSVS DDQASTSITN STDDNANDHD AKRWKGDDEN 240 ESGCGNRAVR EPRVVVQTTS EIDILDDGYR WRKYGQKVVK GNPNPRSYYK CTSNGCPVRK 300 HVERAFHDNR AVITTYEGKH NHEVPAARGS ACYAASRPQA AAYTVQNSDS FAFGNNSYMN 360 QRDNAMSAAA KEEPRDHSFF DLFLD

Sequence ? help Back to Top

Protein Sequence Length: 385 aa Download sequence Send to blast

LMASSGGSLN PALNSFHYNP TSQYMNSFTD LLTSNKDHYE EKYSFPPLSP PPLSPSFFLD  60
SPALFGSSNA LPSPTTGAFA ALFAKDDGPA FSDFSFHSQT RPSDPSAAPI TSKDCVKRPR  120
EEESWIRKVE EVPRSYVREQ GKSDDGYNWR KYGQKQVKGS ENPRSYYKCT FANCPTKKKV  180
ERNLDGCVTE IVYKGSHNHG KPHANSPSVS DDQASTSITN STDDNANDHD AKRWKGDDEN  240
ESGCGNRAVR EPRVVVQTTS EIDILDDGYR WRKYGQKVVK GNPNPRSYYK CTSNGCPVRK  300
HVERAFHDNR AVITTYEGKH NHEVPAARGS ACYAASRPQA AAYTVQNSDS FAFGNNSYMN  360
QRDNAMSAAA KEEPRDHSFF DLFLD

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1wj2_A	2e-36	144	326	8	78	Probable WRKY transcription factor 4
2lex_A	2e-36	144	326	8	78	Probable WRKY transcription factor 4
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Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Interacts specifically with the W box (5'-TTGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Involved in defense responses. Required for resistance to the necrotrophic fungal pathogen B.cinerea (PubMed:17059405, PubMed:21990940). Regulates the antagonistic relationship between defense pathways mediating responses to the bacterial pathogen P. syringae and the necrotrophic pathogen B.cinerea (PubMed:17059405). Required for the phytoalexin camalexin synthesis following infection with B.cinerea. Acts as positive regulator of the camalexin biosynthetic genes PAD3 (CYP71B15) and CYP71A13 by binding to their promoters (PubMed:21498677, PubMed:22392279). Acts downstream of MPK3 and MPK6 in reprogramming the expression of camalexin biosynthetic genes, which drives the metabolic flow to camalexin production (PubMed:21498677). Functions with WRKY25 as positive regulator of salt stress response and abscisic acid (ABA) signaling (PubMed:18839316). Functions with WRKY25 and WRKY26 as positive regulator of plant thermotolerance by partially participating in ethylene-response signal transduction pathway (PubMed:21336597). The DNA-binding activity of WRKY33 is increased by SIB1 and SIB2 (PubMed:21990940). {ECO:0000269\|PubMed:18839316, ECO:0000269\|PubMed:21336597, ECO:0000269\|PubMed:21498677, ECO:0000269\|PubMed:21990940, ECO:0000269\|PubMed:22392279}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By salt stress (PubMed:18839316). Induced by infection with the necrotrophic fungal pathogen B.cinerea (PubMed:17059405, PubMed:21498677, PubMed:21990940). Induced by infection with the bacterial pathogen P.syringae pv. tomato DC3000 (PubMed:17059405). {ECO:0000269\|PubMed:17059405, ECO:0000269\|PubMed:18839316, ECO:0000269\|PubMed:21498677, ECO:0000269\|PubMed:21990940}.

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	KM823162	0.0	KM823162.1 Salvia miltiorrhiza WRKY protein (WRKY39) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_012832231.1	1e-134	PREDICTED: probable WRKY transcription factor 26
Swissprot	Q8S8P5	4e-97	WRK33_ARATH; Probable WRKY transcription factor 33
TrEMBL	A0A0D5YA24	0.0	A0A0D5YA24_SALMI; WRKY protein
STRING	Migut.J00088.1.p	1e-133	(Erythranthe guttata)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Asterids	OGEA2286	24	60

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT2G38470.1	6e-94	WRKY DNA-binding protein 33

Publications ? help Back to Top
Brand LH,Kirchler T,Hummel S,Chaban C,Wanke D DPI-ELISA: a fast and versatile method to specify the binding of plant transcription factors to DNA in vitro. Plant Methods, 2010. 6: p. 25 [PMID:21108821] Brand LH, et al. Screening for protein-DNA interactions by automatable DNA-protein interaction ELISA. PLoS ONE, 2013. 8(10): p. e75177 [PMID:24146751] Ali MA,Wieczorek K,Kreil DP,Bohlmann H The beet cyst nematode Heterodera schachtii modulates the expression of WRKY transcription factors in syncytia to favour its development in Arabidopsis roots. PLoS ONE, 2014. 9(7): p. e102360 [PMID:25033038] Divi UK,Rahman T,Krishna P Gene expression and functional analyses in brassinosteroid-mediated stress tolerance. Plant Biotechnol. J., 2016. 14(1): p. 419-32 [PMID:25973891] Peskan-Berghöfer T, et al. Sustained exposure to abscisic acid enhances the colonization potential of the mutualist fungus Piriformospora indica on Arabidopsis thaliana roots. New Phytol., 2015. 208(3): p. 873-86 [PMID:26075497] Wang C, et al. The Arabidopsis Mediator Complex Subunit16 Is a Key Component of Basal Resistance against the Necrotrophic Fungal Pathogen Sclerotinia sclerotiorum. Plant Physiol., 2015. 169(1): p. 856-72 [PMID:26143252] Wang C, et al. Arabidopsis Elongator subunit 2 positively contributes to resistance to the necrotrophic fungal pathogens Botrytis cinerea and Alternaria brassicicola. Plant J., 2015. 83(6): p. 1019-33 [PMID:26216741] Datta R, et al. Glutathione Regulates 1-Aminocyclopropane-1-Carboxylate Synthase Transcription via WRKY33 and 1-Aminocyclopropane-1-Carboxylate Oxidase by Modulating Messenger RNA Stability to Induce Ethylene Synthesis during Stress. Plant Physiol., 2015. 169(4): p. 2963-81 [PMID:26463088] Daumann M,Fischer M,Niopek-Witz S,Girke C,Möhlmann T Apoplastic Nucleoside Accumulation in Arabidopsis Leads to Reduced Photosynthetic Performance and Increased Susceptibility Against Botrytis cinerea. Front Plant Sci, 2015. 6: p. 1158 [PMID:26779190] Liu S,Bartnikas LM,Volko SM,Ausubel FM,Tang D Mutation of the Glucosinolate Biosynthesis Enzyme Cytochrome P450 83A1 Monooxygenase Increases Camalexin Accumulation and Powdery Mildew Resistance. Front Plant Sci, 2016. 7: p. 227 [PMID:26973671] Jiang Y,Yu D The WRKY57 Transcription Factor Affects the Expression of Jasmonate ZIM-Domain Genes Transcriptionally to Compromise Botrytis cinerea Resistance. Plant Physiol., 2016. 171(4): p. 2771-82 [PMID:27268959] Liao CJ,Lai Z,Lee S,Yun DJ,Mengiste T Arabidopsis HOOKLESS1 Regulates Responses to Pathogens and Abscisic Acid through Interaction with MED18 and Acetylation of WRKY33 and ABI5 Chromatin. Plant Cell, 2016. 28(7): p. 1662-81 [PMID:27317674] Birkenbihl RP,Kracher B,Roccaro M,Somssich IE Induced Genome-Wide Binding of Three Arabidopsis WRKY Transcription Factors during Early MAMP-Triggered Immunity. Plant Cell, 2017. 29(1): p. 20-38 [PMID:28011690] Nguyen CC, et al. Overexpression of oligouridylate binding protein 1b results in ABA hypersensitivity. Plant Signal Behav, 2017. 12(2): p. e1282591 [PMID:28112571] Liu S,Ziegler J,Zeier J,Birkenbihl RP,Somssich IE Botrytis cinerea B05.10 promotes disease development in Arabidopsis by suppressing WRKY33-mediated host immunity. Plant Cell Environ., 2017. 40(10): p. 2189-2206 [PMID:28708934] D'Ambrosio JM, et al. Phospholipase C2 Affects MAMP-Triggered Immunity by Modulating ROS Production. Plant Physiol., 2017. 175(2): p. 970-981 [PMID:28827453] Liu F, et al. Interactions of WRKY15 and WRKY33 transcription factors and their roles in the resistance of oilseed rape to Sclerotinia infection. Plant Biotechnol. J., 2018. 16(4): p. 911-925 [PMID:28929638] Crespo-Salvador Ó,Escamilla-Aguilar M,López-Cruz J,López-Rodas G,González-Bosch C Determination of histone epigenetic marks in Arabidopsis and tomato genes in the early response to Botrytis cinerea. Plant Cell Rep., 2018. 37(1): p. 153-166 [PMID:29119291]