PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Rsa1.0_00313.1_g00009.1

Organism

Raphanus sativus

Taxonomic ID

3726

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Raphanus

Family

WRKY

Protein Properties

Length: 268aa MW: 30043.4 Da PI: 6.2291

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Rsa1.0_00313.1_g00009.1	genome	RGD	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	80.5	1.7e-25	125	185	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-ST...T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsa...gCpvkkkversaedpkvveitYegeHnhe 59 +D ++WrKYG+Ke+ +++fprsY+rCt++ gC+++k+v++ + + k++ itY g+H+++ Rsa1.0_00313.1_g00009.1 125 EDAFSWRKYGKKEILNAKFPRSYFRCTHKytqGCKATKQVQKLELESKMFSITYIGNHTCN 185 8*************************99999************************97 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	7.6E-25	112	185	IPR003657	WRKY domain
SuperFamily	SSF118290	9.81E-23	118	186	IPR003657	WRKY domain
PROSITE profile	PS50811	20.025	119	187	IPR003657	WRKY domain
SMART	SM00774	5.2E-36	124	186	IPR003657	WRKY domain
Pfam	PF03106	2.7E-23	125	184	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 268 aa Download sequence Send to blast
MDIASNNKAI MLEVIDQLVQ GHELATKLQQ LLSQHGSGLG PAEDLVAKIS GSFSDSISAL 60 DSIEHIPSSL FTAVEGSQNA SCNNDGKLED SVDSRKRLGP VKNKRGCYKR KKRADTWTVE 120 STLLEDAFSW RKYGKKEILN AKFPRSYFRC THKYTQGCKA TKQVQKLELE SKMFSITYIG 180 NHTCNSDEVT PKCPSLTTSV NEEENNHHNG SSTESDLQLV WQEMLVFEEE HNHHHHEAIY 240 GCGENSASIN GLDTTDLWSW QQLSVQPQ

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
5w3x_B	5e-20	126	182	18	74	Disease resistance protein RRS1
5w3x_D	5e-20	126	182	18	74	Disease resistance protein RRS1
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Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	94	112	RKRLGPVKNKRGCYKRKKR

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor involved in senescence, biotic and abiotic stress responses by modulating various phytohormones signaling pathways (PubMed:14742872, PubMed:16623907, PubMed:17310369, PubMed:28576847). Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). Binds to the 5'-[CT]GACTTTT-3' motif in promoters of target genes to induce their expression (PubMed:24104863). Plays an important but not indispensable role in jasmonate and salicylic acid signaling (PubMed:18713432). Regulates positively the salicylic acid (SA)-mediated signal pathway, but negatively the jasmonic acid (JA)-mediated signal pathway, thus determining the balance between these mutually antagonistic pathways (PubMed:14742872, PubMed:16623907, PubMed:18713432, PubMed:28837631). Together with WRKY46, WRKY53 and WRKY54, prevents defense response to the necrotrophic pathogens P.carotovorum and B.cinerea, but promotes defense responses (including SA-induced pathogenesis-related (PR) genes expression) against biotrophic/hemibiotrophic SA-monitored pathogens (e.g. P.syringae, E.carotovora subsp. carotovora SCC3193 and E.cichoracearum), probably by regulating negatively the JA/ET and positively the SA signaling pathways (PubMed:28837631, PubMed:16623907, PubMed:22325892). Contributes to the suppression of jasmonic acid (MeJA)-induced expression of JA-responsive genes (e.g. PDF1.2) (PubMed:22325892, PubMed:16623907). Promotes susceptibility to JA-monitored pathogens (e.g. A.brassicicola), probably by facilitating SA-controlled suppression of JA-mediated defense. Represses the biosynthesis of the phytoalexin camalexin and indol-3-ylmethyl glucosinolate (IGS) (PubMed:16623907). Represses both SA and JA/ethylene (ET) mediated defense marker genes expression (PubMed:17310369). Negative regulator of SA biosynthesis (PubMed:28837631). Negative regulator of EDS1-dependent defense against E.amylovora (PubMed:22316300). Required for RPP4-mediated disease resistance and basal defense against H.parasitica, probably via late up-regulation (LURP) of resistance genes (e.g. CML10/CaBP22 and LURP1) (PubMed:17313163). Probably involved in defense responses toward insects (e.g. P.xylostella and B.brassicae) (PubMed:25339349). Together with WRKY54, negative regulator of developmental senescence, probably via the regulation of several senescence-associated markers genes (PubMed:17310369, PubMed:22268143). Together with WRKY46 and WRKY54, promotes brassinosteroid (BR)-regulated plant growth but prevent drought response by modulating gene expression (PubMed:28576847). In collaboration with WRKY54, prevents stomatal closure and, consequently, osmotic stress tolerance (PubMed:23815736). Regulates rhizobacterium B.cereus AR156-induced systemic resistance (ISR) to P.syringae pv. tomato DC3000 (PubMed:26433201). {ECO:0000250\|UniProtKB:Q9SUP6, ECO:0000269\|PubMed:14742872, ECO:0000269\|PubMed:16623907, ECO:0000269\|PubMed:17310369, ECO:0000269\|PubMed:17313163, ECO:0000269\|PubMed:18713432, ECO:0000269\|PubMed:22268143, ECO:0000269\|PubMed:22316300, ECO:0000269\|PubMed:22325892, ECO:0000269\|PubMed:23815736, ECO:0000269\|PubMed:24104863, ECO:0000269\|PubMed:25339349, ECO:0000269\|PubMed:26433201, ECO:0000269\|PubMed:28576847, ECO:0000269\|PubMed:28837631, ECO:0000303\|PubMed:28837631}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Rsa1.0_00313.1_g00009.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Regulated by MYB44 (PubMed:23067202, PubMed:23603962). Basal expression levels require the presence of endogeneous salicylic acid (SA) (PubMed:14742872, PubMed:17310369). Induced by reactive oxygen species (ROS) (PubMed:22268143). Early but transient accumulation after osmotic stress (e.g. polyethylene glycol, PEG) (PubMed:23815736). Induced by SA; early induction is NPR1-independent, but full-scale induction is NPR1-dependent (PubMed:14742872, PubMed:22325892, PubMed:22268143, PubMed:26433201). Up-regulated by benzothiadiazole (BTH) (PubMed:26433201). Repressed by jasmonic acid (MeJA) by both COI1-dependent and COI1-independent pathways (PubMed:14742872, PubMed:18713432). Triggered by the pathogenic compatible bacteria E.carotovora subsp. carotovora SCC3193 (PubMed:14742872). Induced by P.syringae pv. tomato DC3000 (PubMed:17965588, PubMed:22325892). Stimulated by ATX1 (PubMed:17965588). Up-regulated by E.amylovora (PubMed:22316300). Accumulates during leaf and flower senescence (PubMed:17310369). Induced expression upon simultaneous feeding by caterpillars (e.g. P.xylostella) and aphids (e.g. B.brassicae) at a low density, but lower levels in plants induced with both caterpillars and a high aphid density (PubMed:25339349). Responsive to rhizobacterium B.cereus AR156 in leaves (PubMed:26433201). {ECO:0000269\|PubMed:14742872, ECO:0000269\|PubMed:17310369, ECO:0000269\|PubMed:17965588, ECO:0000269\|PubMed:18713432, ECO:0000269\|PubMed:22268143, ECO:0000269\|PubMed:22316300, ECO:0000269\|PubMed:22325892, ECO:0000269\|PubMed:23067202, ECO:0000269\|PubMed:23603962, ECO:0000269\|PubMed:23815736, ECO:0000269\|PubMed:25339349, ECO:0000269\|PubMed:26433201}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_018467131.1	0.0	PREDICTED: probable WRKY transcription factor 70
Swissprot	Q9LY00	1e-116	WRK70_ARATH; Probable WRKY transcription factor 70
TrEMBL	A0A0D2ZS64	1e-172	A0A0D2ZS64_BRAOL; Uncharacterized protein
TrEMBL	A0A3N6QYV0	1e-172	A0A3N6QYV0_BRACR; Uncharacterized protein
STRING	Bo00913s010.1	1e-173	(Brassica oleracea)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM4061	27	57

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT3G56400.1	3e-91	WRKY DNA-binding protein 70

Publications ? help Back to Top
Zhang X, et al. Arabidopsis cysteine-rich receptor-like kinase 45 positively regulates disease resistance to Pseudomonas syringae. Plant Physiol. Biochem., 2013. 73: p. 383-91 [PMID:24215930] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Brosché M, et al. Transcriptomics and functional genomics of ROS-induced cell death regulation by RADICAL-INDUCED CELL DEATH1. PLoS Genet., 2014. 10(2): p. e1004112 [PMID:24550736] Bao F, et al. Arabidopsis HSP90 protein modulates RPP4-mediated temperature-dependent cell death and defense responses. New Phytol., 2014. 202(4): p. 1320-34 [PMID:24611624] Wang F, et al. TaNAC1 acts as a negative regulator of stripe rust resistance in wheat, enhances susceptibility to Pseudomonas syringae, and promotes lateral root development in transgenic Arabidopsis thaliana. Front Plant Sci, 2015. 6: p. 108 [PMID:25774162] Jiang CH, et al. Transcription factors WRKY70 and WRKY11 served as regulators in rhizobacterium Bacillus cereus AR156-induced systemic resistance to Pseudomonas syringae pv. tomato DC3000 in Arabidopsis. J. Exp. Bot., 2016. 67(1): p. 157-74 [PMID:26433201] Kaurilind E,Xu E,Brosché M A genetic framework for H2O2 induced cell death in Arabidopsis thaliana. BMC Genomics, 2015. 16: p. 837 [PMID:26493993] Rodriguez-Salus M, et al. The Synthetic Elicitor 2-(5-Bromo-2-Hydroxy-Phenyl)-Thiazolidine-4-Carboxylic Acid Links Plant Immunity to Hormesis. Plant Physiol., 2016. 170(1): p. 444-58 [PMID:26530314] Onkokesung N,Reichelt M,van Doorn A,Schuurink RC,Dicke M Differential Costs of Two Distinct Resistance Mechanisms Induced by Different Herbivore Species in Arabidopsis. Plant Physiol., 2016. 170(2): p. 891-906 [PMID:26603653] Chen J, et al. Arabidopsis WRKY46, WRKY54, and WRKY70 Transcription Factors Are Involved in Brassinosteroid-Regulated Plant Growth and Drought Responses. Plant Cell, 2017. 29(6): p. 1425-1439 [PMID:28576847] Li J,Zhong R,Palva ET WRKY70 and its homolog WRKY54 negatively modulate the cell wall-associated defenses to necrotrophic pathogens in Arabidopsis. PLoS ONE, 2017. 12(8): p. e0183731 [PMID:28837631] Zhou M, et al. WRKY70 prevents axenic activation of plant immunity by direct repression of SARD1. New Phytol., 2018. 217(2): p. 700-712 [PMID:29076543]