PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

RrC6604_p1

Organism

Raphanus raphanistrum

Taxonomic ID

109996

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Raphanus

Family

WRKY

Protein Properties

Length: 322aa MW: 36687.3 Da PI: 8.6232

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
RrC6604_p1	genome	MSU	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	104.2	6.9e-33	180	237	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhe 59 +Dgy+WrKYGqK vk+s++prsYYrCt+++C+vkk+vers +dp+vv++tYe++Hnh+ RrC6604_p1 180 EDGYRWRKYGQKAVKNSPYPRSYYRCTTQKCNVKKRVERSYQDPTVVITTYESQHNHP 237 8********************************************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PIRSF	PIRSF038130	4.9E-99	1	322	IPR017396	PWRKY transcription factor, group IIc
Gene3D	G3DSA:2.20.25.80	1.2E-34	164	239	IPR003657	WRKY domain
SuperFamily	SSF118290	5.49E-29	171	239	IPR003657	WRKY domain
PROSITE profile	PS50811	32.282	174	239	IPR003657	WRKY domain
SMART	SM00774	6.1E-38	179	238	IPR003657	WRKY domain
Pfam	PF03106	2.0E-25	180	237	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009737	Biological Process	response to abscisic acid
GO:0042742	Biological Process	defense response to bacterium
GO:0050832	Biological Process	defense response to fungus
GO:0051607	Biological Process	defense response to virus
GO:0070301	Biological Process	cellular response to hydrogen peroxide
GO:1901002	Biological Process	positive regulation of response to salt stress
GO:0005634	Cellular Component	nucleus
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 322 aa Download sequence Send to blast
MSNETKDLKN YDYTSSYNHY NNIDSQNMMI LPYVSGPCTY GANMISSQTD SDLHSSSHGA 60 YGFGFHPSPS STEFFNPSID QENGFYNAYN YNTSLKSPEV VGGGGAIIEN ETRVSASPSS 120 SEARRHNGED SGKSLMKREA GDGGDEQRSQ KVVKAKKNQE KKIREPRVSF MTKTEVDHLE 180 DGYRWRKYGQ KAVKNSPYPR SYYRCTTQKC NVKKRVERSY QDPTVVITTY ESQHNHPIPT 240 TRRTAMFSGP AASDYKSSSL SPVSNFINTP RSFSHDDLFR VPYSSVNANP YYQQQQNHEF 300 QHDSDYKLLK EMFPSVFFKQ EP

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1wj2_A	5e-28	169	239	7	77	Probable WRKY transcription factor 4
2lex_A	5e-28	169	239	7	77	Probable WRKY transcription factor 4
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Interacts specifically with the W box (5'-TTGAC[CT]-3'), a frequently occurring stress-responsive cis-acting element. Functions as positive regulator of salt stress response. Binds the W box of LTI78/RD29A stress-response gene and directly regulates its transcription under salt stress. Functions antagonistically with VQ9 to regulate sodium and potassium homeostasis under salt stress by regulating the expression of downstream SOS (SALT OVERLY SENSITIVE) stress-responsive genes. The DNA-binding activity of WRKY8 is decreased by VQ9 (PubMed:23451802). Functions as negative regulator of basal resistance to the bacterial pathogen P. syringae and as positive regulator of resistance to the fungal pathogen to B. cinerea (PubMed:20367464). Functions as positive regulator of defense response againt tobamovirus (TMV) by regulating both the abscisic acid and ethylene signaling pathways. Positively regulates ABI4 expression and negatively modulates ACS6 and ERF104 expression by directly binding to the W box consensus motifs within their promoters (PubMed:23650359). {ECO:0000269\|PubMed:20367464, ECO:0000269\|PubMed:23451802, ECO:0000269\|PubMed:23650359}.

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00545	DAP	Transfer from AT5G46350	Download

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By wounding, abscisic acid (ABA), salicylic acid (SA), H(2)O(2), and infection with P.syringae pv. tomato DC3000 and B.cinerea (PubMed:20367464). Induced by salt stress (PubMed:23451802). {ECO:0000269\|PubMed:20367464, ECO:0000269\|PubMed:23451802}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	EU912418	0.0	EU912418.1 Brassica napus WRKY8-1 transcription factor (WRKY8-1) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_018456683.1	0.0	PREDICTED: probable WRKY transcription factor 8
Swissprot	Q9FL26	1e-154	WRKY8_ARATH; WRKY transcription factor 8
TrEMBL	M4DM30	0.0	M4DM30_BRARP; WRKY transcription factor
STRING	Bra017561.1-P	0.0	(Brassica rapa)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM1524	26	93

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT5G46350.1	1e-151	WRKY DNA-binding protein 8

Publications ? help Back to Top
Duarte JM, et al. Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. Mol. Biol. Evol., 2006. 23(2): p. 469-78 [PMID:16280546] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Sukweenadhi J, et al. Paenibacillus yonginensis DCY84(T) induces changes in Arabidopsis thaliana gene expression against aluminum, drought, and salt stress. Microbiol. Res., 2015. 172: p. 7-15 [PMID:25721473]