PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID Medtr3g072710.1
Common NameNSP2
Organism
Medicago truncatula
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; Trifolieae; Medicago
Family GRAS
Protein Properties Length: 509aa    MW: 56337 Da    PI: 6.0865
Description GRAS family protein
Gene Model
Gene Model ID Type Source Coding Sequence
Medtr3g072710.1genomeMtView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1GRAS3321.1e-1011175001374
             GRAS   1 lvelLlecAeavss..gdlelaqalLarlselaspdg..dpmqRlaayfteALaarlarsvselykalppsetsekn..........sseelaa 80 
                      lv+lL++ Aea++   ++  la+ +L rl+el+s+++  ++m+Rlaa+fteAL+  l + +    +  + ++++  +          + ++laa
  Medtr3g072710.1 117 LVHLLMAGAEALTGstKNRDLARVILIRLKELVSQHAngSNMERLAAHFTEALHGLLEGAGGAHNNHHHHNNNK--HylttngphdnQNDTLAA 208
                      689********976337778************888763379****************99443333222222222..1224456777799***** PP

             GRAS  81 lklfsevsPilkfshltaNqaIleavegeervHiiDfdisqGlQWpaLlqaLasRpegppslRiTgvgspesg..skeeleetgerLakfAeel 172
                      ++l++++sP++kf+h+taNqaI eav++e+rvH+iD+di++G+QW++L+q+Las ++g p+lRiT++++  +g  s  +++etg+rL++fA++l
  Medtr3g072710.1 209 FQLLQDMSPYVKFGHFTANQAIIEAVAHERRVHVIDYDIMEGVQWASLIQSLASNNNG-PHLRITALSRTGTGrrSIATVQETGRRLTSFAASL 301
                      ******************************************************8766.9*********888799999**************** PP

             GRAS 173 gvpfefnvlvakrledleleeLrvkpgEalaVnlvlqlhrlldesvsleserdevLklvkslsPkvvvvveqeadhnsesFlerflealeyysa 266
                      g pf+f+    ++ e++++++L++ +gEal+ n++l+l +l  +  ++     ++L+  k+l+Pk+v++ve+e+    + F+erf+++l++ysa
  Medtr3g072710.1 302 GQPFSFHHCRLDSDETFRPSALKLVRGEALVFNCMLNLPHLSYRAPESV---ASFLNGAKTLNPKLVTLVEEEVGSVIGGFVERFMDSLHHYSA 392
                      ********9999****************************985554444...59**************************************** PP

             GRAS 267 lfdsleaklpreseerikvErellgreivnvvacegaerrerhetlekWrerleeaGFkpvplsekaakqaklllrkvksdgyrveee...sgs 357
                      +fdslea++p ++++r+ vEr+++g++i   +    +     +e+ ++W e+l+e+GF+ vp+s  + +qaklll  ++ dgyrvee    s+ 
  Medtr3g072710.1 393 VFDSLEAGFPMQNRARTLVERVFFGPRIAGSLGRIYRT--GGEEERRSWGEWLGEVGFRGVPVSFANHCQAKLLLGLFN-DGYRVEEVgvgSNK 483
                      ******************************99988776..777888*********************************.******88777*** PP

             GRAS 358 lvlgWkdrpLvsvSaWr 374
                      lvl Wk+r+L+s+S W+
  Medtr3g072710.1 484 LVLDWKSRRLLSASLWT 500
                      ****************6 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PROSITE profilePS5098550.95391478IPR005202Transcription factor GRAS
PfamPF035143.9E-99117500IPR005202Transcription factor GRAS
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009877Biological Processnodulation
GO:0005783Cellular Componentendoplasmic reticulum
GO:0031965Cellular Componentnuclear membrane
Sequence ? help Back to Top
Protein Sequence    Length: 509 aa     Download sequence    Send to blast
MDLMDMDAIN DLHFSGHSSL TNTPTSDEDY GCTWNHWSPI VNWDTFTGAP DDFHHLMDTI  60
IEDRTTVLEQ LSPSITTTTT TTTTTDEEEE EMETTTTTTT TAIKTHEVGD DSKGLKLVHL  120
LMAGAEALTG STKNRDLARV ILIRLKELVS QHANGSNMER LAAHFTEALH GLLEGAGGAH  180
NNHHHHNNNK HYLTTNGPHD NQNDTLAAFQ LLQDMSPYVK FGHFTANQAI IEAVAHERRV  240
HVIDYDIMEG VQWASLIQSL ASNNNGPHLR ITALSRTGTG RRSIATVQET GRRLTSFAAS  300
LGQPFSFHHC RLDSDETFRP SALKLVRGEA LVFNCMLNLP HLSYRAPESV ASFLNGAKTL  360
NPKLVTLVEE EVGSVIGGFV ERFMDSLHHY SAVFDSLEAG FPMQNRARTL VERVFFGPRI  420
AGSLGRIYRT GGEEERRSWG EWLGEVGFRG VPVSFANHCQ AKLLLGLFND GYRVEEVGVG  480
SNKLVLDWKS RRLLSASLWT CSSSDSDL*
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
5b3h_A1e-5111050311381Protein SCARECROW
5b3h_D1e-5111050311381Protein SCARECROW
Search in ModeBase
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Expressed in roots, shoots and leaves. {ECO:0000269|PubMed:15961668}.
Functional Description ? help Back to Top
Source Description
UniProtTranscriptional regulator essential for Nod-factor-induced gene expression. Acts downstream of calcium spiking and DMI3, a calcium/calmodulin-dependent protein kinase (CCaMK). {ECO:0000269|PubMed:15961668}.
Function -- GeneRIF ? help Back to Top
  1. NSP1 and NSP2 are single-copy genes in legumes, which implies that both proteins fulfill dual regulatory functions to control downstream targets after rhizobium-induced signaling as well as strigolactone biosynthesis in nonsymbiotic conditions.
    [PMID: 22039214]
  2. A regulatory mechanism is triggered by lipochito-oligosaccharides (Myc-LCOs), that prevents over-colonization of roots by arbuscular mycorrhizal fungi by a mechanism involving miRNA-mediated negative regulation of NSP2.
    [PMID: 22775306]
  3. Two transcription factors (TFs), NSP2 and bHLH476, are direct cytokinin targets acting at the convergence of phytohormonal and symbiotic cues.
    [PMID: 23023168]
Cis-element ? help Back to Top
SourceLink
PlantRegMapMedtr3g072710.1
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Induced 24 hours after treatment with Nod factor or Sinorhizobium meliloti. {ECO:0000269|PubMed:15961668}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAJ8321380.0AJ832138.1 Medicago truncatula nsp2 gene for GRAS family protein.
GenBankCR5387220.0CR538722.1 M.truncatula DNA sequence from clone MTH2-11A20 on chromosome 3, complete sequence.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_003601076.10.0nodulation-signaling pathway 2 protein
SwissprotQ5NE240.0NSP2_MEDTR; Nodulation-signaling pathway 2 protein
TrEMBLA0A396IRZ50.0A0A396IRZ5_MEDTR; Nodulation-signaling pathway 2 protein
STRINGAES713270.0(Medicago truncatula)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
FabidsOGEF62643152
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT4G08250.11e-145GRAS family protein
Publications ? help Back to Top
  1. Catoira R, et al.
    Four genes of Medicago truncatula controlling components of a nod factor transduction pathway.
    Plant Cell, 2000. 12(9): p. 1647-66
    [PMID:11006338]
  2. Oldroyd GE,Long SR
    Identification and characterization of nodulation-signaling pathway 2, a gene of Medicago truncatula involved in Nod actor signaling.
    Plant Physiol., 2003. 131(3): p. 1027-32
    [PMID:12644655]
  3. Kal
    Nodulation signaling in legumes requires NSP2, a member of the GRAS family of transcriptional regulators.
    Science, 2005. 308(5729): p. 1786-9
    [PMID:15961668]
  4. Liu W, et al.
    Strigolactone biosynthesis in Medicago truncatula and rice requires the symbiotic GRAS-type transcription factors NSP1 and NSP2.
    Plant Cell, 2011. 23(10): p. 3853-65
    [PMID:22039214]
  5. Young ND, et al.
    The Medicago genome provides insight into the evolution of rhizobial symbioses.
    Nature, 2011. 480(7378): p. 520-4
    [PMID:22089132]
  6. Lauressergues D, et al.
    The microRNA miR171h modulates arbuscular mycorrhizal colonization of Medicago truncatula by targeting NSP2.
    Plant J., 2012. 72(3): p. 512-22
    [PMID:22775306]
  7. Ariel F, et al.
    Two direct targets of cytokinin signaling regulate symbiotic nodulation in Medicago truncatula.
    Plant Cell, 2012. 24(9): p. 3838-52
    [PMID:23023168]
  8. Tang H, et al.
    An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
    BMC Genomics, 2014. 15: p. 312
    [PMID:24767513]