PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Lj4g3v2140040.1

Organism

Lotus japonicus

Taxonomic ID

34305

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; Loteae; Lotus

Family

WRKY

Protein Properties

Length: 310aa MW: 34109.7 Da PI: 10.5756

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Lj4g3v2140040.1	genome	Kazusa	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	100	1.4e-31	241	298	2	58
--SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnh 58 D ++WrKYGqK++kgs++pr+YY+C+s+ gCp++k+ver+++dp+++++tYegeH+h Lj4g3v2140040.1 241 TDDFQWRKYGQKPIKGSPYPRGYYKCSSEkGCPARKHVERAQDDPNMLIVTYEGEHRH 298 699***********************99*************************9 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Pfam	PF10533	6.5E-18	194	238	IPR018872	Zn-cluster domain
Gene3D	G3DSA:2.20.25.80	4.2E-33	228	298	IPR003657	WRKY domain
PROSITE profile	PS50811	30.871	235	301	IPR003657	WRKY domain
SuperFamily	SSF118290	1.11E-26	236	298	IPR003657	WRKY domain
SMART	SM00774	3.8E-38	240	300	IPR003657	WRKY domain
Pfam	PF03106	9.1E-28	242	298	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 310 aa Download sequence Send to blast
MKMEEQIAIQ EAASVGLKSM EHLIRILSSQ TPSFSSSSSS SSSSSHNRHR LDLNNLDCTE 60 ITDFTVSKFK QVINLLNRTG HARFRQAPSP SPAPPPPLSQ PQPEKFNSHQ FNNHHRSTTL 120 DFAKPVMVKS NLKPDLISKP KLSTDLSVSQ YSTTTTTTNS SFMSSITGEG SVSNGKIGLA 180 IVAAGRPPLS SSHRKRCHDA TLTGKTSSSG SRCHCSKKKK LRVKRTIRVP AISSKTADIP 240 TDDFQWRKYG QKPIKGSPYP RGYYKCSSEK GCPARKHVER AQDDPNMLIV TYEGEHRHLI 300 TTTAAVGFMA

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
2ayd_A	9e-22	228	298	2	71	WRKY transcription factor 1
Search in ModeBase

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
Lja.25700	0.0	cell culture\| protoplast\| root

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: In young, mature and senescent leaves. {ECO:0000269\|PubMed:11722756}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). Regulates rhizobacterium B.cereus AR156-induced systemic resistance (ISR) to P.syringae pv. tomato DC3000, probably by activating the jasmonic acid (JA)- signaling pathway (PubMed:26433201). {ECO:0000250, ECO:0000269\|PubMed:26433201}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Lj4g3v2140040.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Strongly during leaf senescence (PubMed:11722756). Repressed by rhizobacterium B.cereus AR156 in leaves, and to a lower extent, by P.fluorescens WCS417r (PubMed:26433201). {ECO:0000269\|PubMed:11722756, ECO:0000269\|PubMed:26433201}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AP006417	0.0	AP006417.1 Lotus japonicus genomic DNA, chromosome 4, clone: LjT05D12, TM0303, complete sequence.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_028197771.1	1e-130	probable WRKY transcription factor 17
Swissprot	Q9SV15	6e-96	WRK11_ARATH; Probable WRKY transcription factor 11
TrEMBL	I1LVK7	1e-129	I1LVK7_SOYBN; Uncharacterized protein
STRING	GLYMA13G00380.1	1e-130	(Glycine max)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Fabids	OGEF1314	34	98

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT4G31550.1	3e-88	WRKY DNA-binding protein 11

Publications ? help Back to Top
Haas BJ, et al. Full-length messenger RNA sequences greatly improve genome annotation. Genome Biol., 2002. 3(6): p. RESEARCH0029 [PMID:12093376] Brand LH,Kirchler T,Hummel S,Chaban C,Wanke D DPI-ELISA: a fast and versatile method to specify the binding of plant transcription factors to DNA in vitro. Plant Methods, 2010. 6: p. 25 [PMID:21108821] Brand LH, et al. Screening for protein-DNA interactions by automatable DNA-protein interaction ELISA. PLoS ONE, 2013. 8(10): p. e75177 [PMID:24146751] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Ali MA,Wieczorek K,Kreil DP,Bohlmann H The beet cyst nematode Heterodera schachtii modulates the expression of WRKY transcription factors in syncytia to favour its development in Arabidopsis roots. PLoS ONE, 2014. 9(7): p. e102360 [PMID:25033038] Jiang CH, et al. Transcription factors WRKY70 and WRKY11 served as regulators in rhizobacterium Bacillus cereus AR156-induced systemic resistance to Pseudomonas syringae pv. tomato DC3000 in Arabidopsis. J. Exp. Bot., 2016. 67(1): p. 157-74 [PMID:26433201] Ali MA, et al. Transcription factors WRKY11 and WRKY17 are involved in abiotic stress responses in Arabidopsis. J. Plant Physiol., 2018. 226: p. 12-21 [PMID:29689430]