PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT4G31550.1
Common NameATWRKY11, F3L17.120, WRKY11
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family WRKY
Protein Properties Length: 325aa    MW: 35804.6 Da    PI: 10.224
Description WRKY DNA-binding protein 11
Gene Model
Gene Model ID Type Source Coding Sequence
AT4G31550.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
         WRKY   3 DgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 
                  D+y+WrKYGqK++kgs++pr+YY+C++  gCp++k+ver+ +dp ++++tYegeH+h+
                  9************************8766****************************8 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PfamPF105331.5E-17194243IPR018872Zn-cluster domain
Gene3DG3DSA: domain
PROSITE profilePS5081130.24240306IPR003657WRKY domain
SuperFamilySSF1182901.57E-26243305IPR003657WRKY domain
SMARTSM007741.8E-37245305IPR003657WRKY domain
PfamPF031067.6E-27247303IPR003657WRKY domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009737Biological Processresponse to abscisic acid
GO:0009938Biological Processnegative regulation of gibberellic acid mediated signaling pathway
GO:0010200Biological Processresponse to chitin
GO:0042742Biological Processdefense response to bacterium
GO:0005634Cellular Componentnucleus
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005516Molecular Functioncalmodulin binding
GO:0043565Molecular Functionsequence-specific DNA binding
GO:1990841Molecular Functionpromoter-specific chromatin binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000084anatomyplant sperm cell
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009025anatomyvascular leaf
PO:0009052anatomyflower pedicel
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 325 aa     Download sequence    Send to blast
3D Structure ? help Back to Top
PDB ID Evalue Query Start Query End Hit Start Hit End Description
2ayd_A2e-20233304272WRKY transcription factor 1
Search in ModeBase
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.246830.0bud| flower| inflorescence| leaf| seed
Expression -- Microarray ? help Back to Top
Source ID E-value
Expression AtlasAT4G31550-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: In young, mature and senescent leaves. {ECO:0000269|PubMed:11722756}.
Functional Description ? help Back to Top
Source Description
TAIRmember of WRKY Transcription Factor; Group II-d; negative regulator of basal resistance to Pseudomonas syringae.
UniProtTranscription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). Regulates rhizobacterium B.cereus AR156-induced systemic resistance (ISR) to P.syringae pv. tomato DC3000, probably by activating the jasmonic acid (JA)- signaling pathway (PubMed:26433201). {ECO:0000250, ECO:0000269|PubMed:26433201}.
Function -- GeneRIF ? help Back to Top
  1. These results provide genetic evidence for the importance of WRKY11 and WRKY17 in plant defense.
    [PMID: 17114354]
  2. Data show that even subtle amino acid substitutions within the DNA binding region of AtWRKY11 strongly impinge on its binding activity.
    [PMID: 18523729]
  3. Bacillus cereus AR156 treatment significantly stimulated the transcription of WRKY70, but suppressed that of WRKY11 in Arabidopsis leaves. They were shown to be required for AR156 enhancing the activation of cellular defence responses.
    [PMID: 26433201]
  4. wrky11 and wrky17 are not strictly limited to plant defense responses but are also involved in conferring stress tolerance.
    [PMID: 29689430]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
Motif logo
Cis-element ? help Back to Top
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Strongly during leaf senescence (PubMed:11722756). Repressed by rhizobacterium B.cereus AR156 in leaves, and to a lower extent, by P.fluorescens WCS417r (PubMed:26433201). {ECO:0000269|PubMed:11722756, ECO:0000269|PubMed:26433201}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
Regulation -- ATRM (Manually Curated Upstream Regulators) ? help Back to Top
Source Upstream Regulator (A: Activate/R: Repress)
ATRM AT2G24570 (A), AT4G31550 (A)
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT2G24570(R), AT2G40750(R), AT3G45140(A), AT3G56400(R), AT4G31550(A), AT5G42650(A)
Interaction ? help Back to Top
Source Intact With
IntActSearch Q9SV15
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT4G31550
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAY1503940.0AY150394.1 Arabidopsis thaliana putative DNA-binding protein (At4g31550) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_849559.10.0WRKY DNA-binding protein 11
SwissprotQ9SV150.0WRK11_ARATH; Probable WRKY transcription factor 11
STRINGAT4G31550.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Representative plantOGRP35571127
Publications ? help Back to Top
  1. Eulgem T,Rushton PJ,Robatzek S,Somssich IE
    The WRKY superfamily of plant transcription factors.
    Trends Plant Sci., 2000. 5(5): p. 199-206
  2. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
  3. Robatzek S,Somssich IE
    A new member of the Arabidopsis WRKY transcription factor family, AtWRKY6, is associated with both senescence- and defence-related processes.
    Plant J., 2001. 28(2): p. 123-33
  4. Haas BJ, et al.
    Full-length messenger RNA sequences greatly improve genome annotation.
    Genome Biol., 2002. 3(6): p. RESEARCH0029
  5. Gutierrez RA,Ewing RM,Cherry JM,Green PJ
    Identification of unstable transcripts in Arabidopsis by cDNA microarray analysis: rapid decay is associated with a group of touch- and specific clock-controlled genes.
    Proc. Natl. Acad. Sci. U.S.A., 2002. 99(17): p. 11513-8
  6. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
  7. Navarro L, et al.
    The transcriptional innate immune response to flg22. Interplay and overlap with Avr gene-dependent defense responses and bacterial pathogenesis.
    Plant Physiol., 2004. 135(2): p. 1113-28
  8. De Paepe A,Vuylsteke M,Van Hummelen P,Zabeau M,Van Der Straeten D
    Transcriptional profiling by cDNA-AFLP and microarray analysis reveals novel insights into the early response to ethylene in Arabidopsis.
    Plant J., 2004. 39(4): p. 537-59
  9. Lu Y,Zhu J,Liu P
    A two-step strategy for detecting differential gene expression in cDNA microarray data.
    Curr. Genet., 2005. 47(2): p. 121-31
  10. Park CY, et al.
    WRKY group IId transcription factors interact with calmodulin.
    FEBS Lett., 2005. 579(6): p. 1545-50
  11. Thilmony R,Underwood W,He SY
    Genome-wide transcriptional analysis of the Arabidopsis thaliana interaction with the plant pathogen Pseudomonas syringae pv. tomato DC3000 and the human pathogen Escherichia coli O157:H7.
    Plant J., 2006. 46(1): p. 34-53
  12. Journot-Catalino N,Somssich IE,Roby D,Kroj T
    The transcription factors WRKY11 and WRKY17 act as negative regulators of basal resistance in Arabidopsis thaliana.
    Plant Cell, 2006. 18(11): p. 3289-302
  13. Ma S,Bohnert HJ
    Integration of Arabidopsis thaliana stress-related transcript profiles, promoter structures, and cell-specific expression.
    Genome Biol., 2007. 8(4): p. R49
  14. Zhang Z, et al.
    Dual regulation role of GH3.5 in salicylic acid and auxin signaling during Arabidopsis-Pseudomonas syringae interaction.
    Plant Physiol., 2007. 145(2): p. 450-64
  15. Libault M,Wan J,Czechowski T,Udvardi M,Stacey G
    Identification of 118 Arabidopsis transcription factor and 30 ubiquitin-ligase genes responding to chitin, a plant-defense elicitor.
    Mol. Plant Microbe Interact., 2007. 20(8): p. 900-11
  16. Ciolkowski I,Wanke D,Birkenbihl RP,Somssich IE
    Studies on DNA-binding selectivity of WRKY transcription factors lend structural clues into WRKY-domain function.
    Plant Mol. Biol., 2008. 68(1-2): p. 81-92
  17. Huang D,Wu W,Abrams SR,Cutler AJ
    The relationship of drought-related gene expression in Arabidopsis thaliana to hormonal and environmental factors.
    J. Exp. Bot., 2008. 59(11): p. 2991-3007
  18. Liu H, et al.
    Ectopic expression of a grapevine transcription factor VvWRKY11 contributes to osmotic stress tolerance in Arabidopsis.
    Mol. Biol. Rep., 2011. 38(1): p. 417-27
  19. Brand LH,Kirchler T,Hummel S,Chaban C,Wanke D
    DPI-ELISA: a fast and versatile method to specify the binding of plant transcription factors to DNA in vitro.
    Plant Methods, 2010. 6: p. 25
  20. Arabidopsis Interactome Mapping Consortium
    Evidence for network evolution in an Arabidopsis interactome map.
    Science, 2011. 333(6042): p. 601-7
  21. Brand LH, et al.
    Screening for protein-DNA interactions by automatable DNA-protein interaction ELISA.
    PLoS ONE, 2013. 8(10): p. e75177
  22. Ding Y, et al.
    Four distinct types of dehydration stress memory genes in Arabidopsis thaliana.
    BMC Plant Biol., 2013. 13: p. 229
  23. Ali MA,Wieczorek K,Kreil DP,Bohlmann H
    The beet cyst nematode Heterodera schachtii modulates the expression of WRKY transcription factors in syncytia to favour its development in Arabidopsis roots.
    PLoS ONE, 2014. 9(7): p. e102360
  24. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
  25. Jiang CH, et al.
    Transcription factors WRKY70 and WRKY11 served as regulators in rhizobacterium Bacillus cereus AR156-induced systemic resistance to Pseudomonas syringae pv. tomato DC3000 in Arabidopsis.
    J. Exp. Bot., 2016. 67(1): p. 157-74
  26. Ali MA, et al.
    Transcription factors WRKY11 and WRKY17 are involved in abiotic stress responses in Arabidopsis.
    J. Plant Physiol., 2018. 226: p. 12-21