PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID Gorai.004G165400.1
Common NameB456_004G165400
Organism
Gossypium raimondii
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium
Family MYB
Protein Properties Length: 416aa    MW: 46495.6 Da    PI: 5.3197
Description MYB family protein
Gene Model
Gene Model ID Type Source Coding Sequence
Gorai.004G165400.1genomeJGIView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1Myb_DNA-binding40.94.7e-13106151146
                         TSSS-HHHHHHHHHHHHHTTTT-HHHHHHHHTTTS-HHHHHHHHHH CS
     Myb_DNA-binding   1 rgrWTteEdellvdavkqlGggtWktIartmgkgRtlkqcksrwqk 46 
                         +g+W +eEde l++ vk++G++ W++I ++  + Rt+k+c++rw +
  Gorai.004G165400.1 106 KGPWKAEEDEVLINHVKKYGPREWSSIRSKGLLQRTGKSCRLRWVN 151
                         79*************************8887799**********87 PP
2Myb_DNA-binding44.82.8e-14162201344
                         SS-HHHHHHHHHHHHHTTTT-HHHHHHHHTTTS-HHHHHHHH CS
     Myb_DNA-binding   3 rWTteEdellvdavkqlGggtWktIartmgkgRtlkqcksrw 44 
                         ++T+eE+   +++ +q+G++ W++Ia +++ gRt++++k++w
  Gorai.004G165400.1 162 KFTAEEERVVIELQAQFGNK-WAKIATYLP-GRTDNDVKNFW 201
                         79******************.*********.*********** PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PROSITE profilePS5129414.714101153IPR017930Myb domain
Gene3DG3DSA:1.10.10.609.5E-21103154IPR009057Homeodomain-like
SuperFamilySSF466893.33E-28104201IPR009057Homeodomain-like
SMARTSM007174.4E-10105155IPR001005SANT/Myb domain
CDDcd001678.56E-11108153No hitNo description
PfamPF139211.8E-11109159No hitNo description
PROSITE profilePS5129420.671154209IPR017930Myb domain
Gene3DG3DSA:1.10.10.601.4E-18155207IPR009057Homeodomain-like
SMARTSM007175.9E-13159207IPR001005SANT/Myb domain
PfamPF002491.9E-12162201IPR001005SANT/Myb domain
CDDcd001673.47E-11162201No hitNo description
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0048235Biological Processpollen sperm cell differentiation
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
Sequence ? help Back to Top
Protein Sequence    Length: 416 aa     Download sequence    Send to blast
VSTNLLMEYM QQLPFDQGAI NKMNDLHLSF DANPPKSSNL HRPQRSLGVG GFDSPWVLCL  60
HISSYLNSNL IGGVVIIQND NGTHFLAEVK LGEMEGKLVE ISQIRKGPWK AEEDEVLINH  120
VKKYGPREWS SIRSKGLLQR TGKSCRLRWV NKLRPNLKNG CKFTAEEERV VIELQAQFGN  180
KWAKIATYLP GRTDNDVKNF WSSRQKRLAR ILQNSGTPSS SSSSSSSKSL KLKREIPAFH  240
DVPVFEAPNL SSSMEEISCT MAQSCSSSSY LDNTETIIKV EQFPKLVNPK LYTDANMAQL  300
ELMSIGNNPY AAAEAQPQAF FPQTPHPQPY LTLSLESQDL LAKFEDPYFS QVFAPMDVPE  360
LDSGNVEQQP FLEPVRSGGF GAREEADNPM IPDAFFDDFP ADMFDQMEPL PNPST*
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
1h8a_C3e-3010320724126MYB TRANSFORMING PROTEIN
Search in ModeBase
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: Strict spatial and temporal transcriptional activation leading to a specific expression in the male germline cells; this specific expression pattern is dependent of the regulatory region of DUO1 (ROD1) 5'-YAACYGY-3' in its promoter (PubMed:15694308, PubMed:27624837). First observed in the germ cell during or soon after engulfment by the vegetative cell, just after asymmetric division. In maturating pollen, accumulates in germ cells before mitosis and remains high in mature sperm cells. Expression persists during pollen development (PubMed:19300502). {ECO:0000269|PubMed:15694308, ECO:0000269|PubMed:19300502, ECO:0000269|PubMed:27624837}.
UniprotTISSUE SPECIFICITY: Confined to inflorescences, especially in stamens and pollen. {ECO:0000269|PubMed:15694308}.
Functional Description ? help Back to Top
Source Description
UniProtTranscription activator that acts as a positive regulator of male germline development by promoting both gametic cell specification and cell cycle progression (PubMed:15694308, PubMed:15618418, PubMed:19300502, PubMed:21285328). Binds to canonical MYB sites 5'-AACCGTC-3', 5'-AAACCGC-3' and 5'-AACCGT-3' in promoters to trigger the expression of male germline-specific or enriched genes (e.g. MGH3, GEX2 and GCS1), including those required for fertilization (PubMed:21285328, PubMed:19300502). Required for sperm cell specification leading to pollen maturation by activating a germline-specific regulon (PubMed:21285328, PubMed:15694308, PubMed:15618418, PubMed:19300502). Involved in pollen mitosis entry at G2-M transition via the regulation of CYCB1-1, DAZ1 and DAZ2 expression (PubMed:15618418, PubMed:19300502, PubMed:24876252). {ECO:0000269|PubMed:15618418, ECO:0000269|PubMed:15694308, ECO:0000269|PubMed:19300502, ECO:0000269|PubMed:21285328, ECO:0000269|PubMed:24876252}.
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Repressed by the microRNA 159 (miR159); the production of miR159 is stimulated by the anaphase promoting complex/cyclosome (APC/C) (PubMed:21441434). Activated by ARID1 in male germline cells via specific histone acetylation regulation (e.g. H3K9Ac) (PubMed:25057814). {ECO:0000269|PubMed:21441434, ECO:0000269|PubMed:25057814}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankJX5884501e-101JX588450.1 Gossypium hirsutum clone NBRI_GE22878 microsatellite sequence.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_012475348.10.0PREDICTED: transcription factor LAF1
SwissprotA0A178VEK75e-85DUO1_ARATH; Transcription factor DUO1
TrEMBLA0A0D2S0S50.0A0A0D2S0S5_GOSRA; Uncharacterized protein (Fragment)
STRINGGorai.004G165400.10.0(Gossypium raimondii)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM65512842
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT3G60460.14e-72MYB family protein
Publications ? help Back to Top
  1. Paterson AH, et al.
    Repeated polyploidization of Gossypium genomes and the evolution of spinnable cotton fibres.
    Nature, 2012. 492(7429): p. 423-7
    [PMID:23257886]
  2. Zheng B,He H,Zheng Y,Wu W,McCormick S
    An ARID domain-containing protein within nuclear bodies is required for sperm cell formation in Arabidopsis thaliana.
    PLoS Genet., 2014. 10(7): p. e1004421
    [PMID:25057814]
  3. Peters B, et al.
    A Conserved cis-Regulatory Module Determines Germline Fate through Activation of the Transcription Factor DUO1 Promoter.
    Plant Physiol., 2017. 173(1): p. 280-293
    [PMID:27624837]
  4. Zheng Z, et al.
    Target RNA Secondary Structure Is a Major Determinant of miR159 Efficacy.
    Plant Physiol., 2017. 174(3): p. 1764-1778
    [PMID:28515145]