PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Gh_A08G1412

Common Name

bHLH1

Organism

Gossypium hirsutum

Taxonomic ID

3635

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium

Family

bHLH

Protein Properties

Length: 674aa MW: 73570.8 Da PI: 5.4262

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Gh_A08G1412	genome	NAU-NBI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	39.3	1.2e-12	499	544	4	54
HHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHH CS HLH 4 ahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksL 54 +h e+Er+RR+++N++f Lr ++P+ K++Ka+ L A+ YI++L Gh_A08G1412 499 NHVEAERQRREKLNQKFYALRAVVPNV-----SKMDKASLLGDAISYINEL 544 799*********************6.....5*************998 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Pfam	PF14215	4.2E-59	82	263	IPR025610	Transcription factor MYC/MYB N-terminal
PROSITE profile	PS50888	17.25	495	544	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamily	SSF47459	1.44E-18	498	567	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	4.83E-15	498	549	No hit	No description
Pfam	PF00010	3.7E-10	499	544	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Gene3D	G3DSA:4.10.280.10	2.7E-18	499	565	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	6.0E-17	501	550	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 674 aa Download sequence Send to blast
MTDYQLAPTM NLWTDDNASV MEAFMTSDLS SIWPPPQSSA STSTPVVAAP PPPPPPAAGL 60 DPSKSFLPHS QPSVSLLSQE SLQQRLQALI EGARESWTYA IFWQSSYDYS ATTVLGWGDG 120 YYKGEEDKGK AKLKASSSSV AEQEHRKKVL RELNSLISGS AAPTDDAVDE EVTDTEWFFL 180 VSMTQSFVDG SGLPGQAFFN SSPVWVAGPD RLESSMCERA KQAQVFGLQT LVCIPSANGV 240 VELGSTELIT QSSDIMNKVR VLFNFNIEIE AGSWCMSNNT ADQGENDPSS LWISDPHAGV 300 EFKESSNTTT TTNHTSNQNQ QTQKSIQFCD NRSSSSLTEN PSSIPAGNHH QQQQSHPQGQ 360 SLCLNFSDYG FDESSSVRNG NSSSHLLKPE SGEILNFGES KRSGNGNLFT GNSPFAVENK 420 KRSPNSRGSN EEAMLSFTSG VILPSSGVVK SSGGAGDSDH SDLEASVVKE ADSSRVVEPE 480 KRPRKRGRKP ANGREEPLNH VEAERQRREK LNQKFYALRA VVPNVSKMDK ASLLGDAISY 540 INELKSKLQS ADLEKEEMQS QLEALKKNLS SKAPPPHDQD LKISNHSGNK LIDLEIEVKI 600 IGWDAMIQIQ CSKKNHPAAK LMAALKELDL DVHHASVSVV KDLMIQQANV KMGSRFFTQE 660 QLKSALTTKL GDAR

Sequence ? help Back to Top

Protein Sequence Length: 674 aa Download sequence Send to blast

MTDYQLAPTM NLWTDDNASV MEAFMTSDLS SIWPPPQSSA STSTPVVAAP PPPPPPAAGL  60
DPSKSFLPHS QPSVSLLSQE SLQQRLQALI EGARESWTYA IFWQSSYDYS ATTVLGWGDG  120
YYKGEEDKGK AKLKASSSSV AEQEHRKKVL RELNSLISGS AAPTDDAVDE EVTDTEWFFL  180
VSMTQSFVDG SGLPGQAFFN SSPVWVAGPD RLESSMCERA KQAQVFGLQT LVCIPSANGV  240
VELGSTELIT QSSDIMNKVR VLFNFNIEIE AGSWCMSNNT ADQGENDPSS LWISDPHAGV  300
EFKESSNTTT TTNHTSNQNQ QTQKSIQFCD NRSSSSLTEN PSSIPAGNHH QQQQSHPQGQ  360
SLCLNFSDYG FDESSSVRNG NSSSHLLKPE SGEILNFGES KRSGNGNLFT GNSPFAVENK  420
KRSPNSRGSN EEAMLSFTSG VILPSSGVVK SSGGAGDSDH SDLEASVVKE ADSSRVVEPE  480
KRPRKRGRKP ANGREEPLNH VEAERQRREK LNQKFYALRA VVPNVSKMDK ASLLGDAISY  540
INELKSKLQS ADLEKEEMQS QLEALKKNLS SKAPPPHDQD LKISNHSGNK LIDLEIEVKI  600
IGWDAMIQIQ CSKKNHPAAK LMAALKELDL DVHHASVSVV KDLMIQQANV KMGSRFFTQE  660
QLKSALTTKL GDAR

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
4rqw_A	5e-77	77	266	4	195	Transcription factor MYC3
4rqw_B	5e-77	77	266	4	195	Transcription factor MYC3
4rs9_A	5e-77	77	266	4	195	Transcription factor MYC3
4yz6_A	5e-77	77	266	4	195	Transcription factor MYC3
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Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	480	488	KRPRKRGRK

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
Ghi.24773	0.0	boll\| ovule\| stem

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Expressed at low levels in roots, stems, leaves, flowers and fruits. {ECO:0000269\|PubMed:15231736}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcriptional activator that binds to the G-box motif (5'-AACGTG-3') found in the promoter of the jasmonate-induced gene LAPA1 (PubMed:15231736). Acts as negative regulator of blue light-mediated photomorphogenesis and positively regulates root growth (PubMed:24483714). Promotes growth in response to the phytohormones abscisic acid (ABA) and jasmonate (JA) (PubMed:24483714). Binds to the G-box motif (5'-CACGTG-3') of the RBCS-3A gene promoter (PubMed:24483714). Acts downstream of the jasmonate (JA) receptor to orchestrate JA-mediated activation of plant responses (PubMed:28733419). Positively regulates both wound-responsive and pathogen-responsive genes through MYC2-targeted transcription factors (MTFs) involved in early response to JA (PubMed:28733419). With JA2L forms a transcription module that regulates wounding-responsive genes (PubMed:28733419). With ERF.C3 forms a transcription module that regulates pathogen-responsive genes (PubMed:28733419). Plays a critical role in orchestrating JA-mediated defense gene expression during Botrytis cinerea infection (PubMed:28733419). Regulates negatively defense responses to root-knot nematodes, potentially by mediating crosstalk among the hormones strigolactones, abscisic acid (ABA) and jasmonate (JA) (PubMed:30576511). Regulates the termination of JA-mediated defense responses by specifically binding the G-box (5'-CACATG-3') motifs in the promoters of MTB1, MTB2 and MTB3, which are transcription factors that negatively regulates JA signaling (PubMed:30610166). May be involved in JA-induced chilling tolerance, possibly by ameliorating the antioxidant enzyme system of fruit and increasing proline and lycopene levels (PubMed:29528226). {ECO:0000269\|PubMed:15231736, ECO:0000269\|PubMed:24483714, ECO:0000269\|PubMed:28733419, ECO:0000269\|PubMed:29528226, ECO:0000269\|PubMed:30576511, ECO:0000269\|PubMed:30610166}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Induced by methyl jasmonate (PubMed:15231736, PubMed:28733419). Induced by wounding (PubMed:15231736). Induced by infection with the fungal pathogen Botrytis cinerea (PubMed:28733419). Induced in fruit by storage in cold (PubMed:29528226). Induced by hydrogen peroxide and infection with root-knot nematodes (PubMed:30576511). {ECO:0000269\|PubMed:15231736, ECO:0000269\|PubMed:28733419, ECO:0000269\|PubMed:29528226, ECO:0000269\|PubMed:30576511}.

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	FJ358540	0.0	FJ358540.1 Gossypium hirsutum bHLH domain protein (bHLH1) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_016704890.1	0.0	PREDICTED: transcription factor MYC2-like
Swissprot	A0A3Q7HRZ6	0.0	MYC2_SOLLC; Transcription factor MYC2
TrEMBL	A0A1U8KR28	0.0	A0A1U8KR28_GOSHI; transcription factor MYC2-like
STRING	Gorai.004G184800.1	0.0	(Gossypium raimondii)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM1265	28	96

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT1G32640.1	1e-179	bHLH family protein

Publications ? help Back to Top
Tomato Genome Consortium The tomato genome sequence provides insights into fleshy fruit evolution. Nature, 2012. 485(7400): p. 635-41 [PMID:22660326] Gupta N,Prasad VB,Chattopadhyay S LeMYC2 acts as a negative regulator of blue light mediated photomorphogenic growth, and promotes the growth of adult tomato plants. BMC Plant Biol., 2014. 14: p. 38 [PMID:24483714] Sun H,Fan HJ,Ling HQ Genome-wide identification and characterization of the bHLH gene family in tomato. BMC Genomics, 2015. 16: p. 9 [PMID:25612924] Du M, et al. MYC2 Orchestrates a Hierarchical Transcriptional Cascade That Regulates Jasmonate-Mediated Plant Immunity in Tomato. Plant Cell, 2017. 29(8): p. 1883-1906 [PMID:28733419] Min D, et al. SlMYC2 Involved in Methyl Jasmonate-Induced Tomato Fruit Chilling Tolerance. J. Agric. Food Chem., 2018. 66(12): p. 3110-3117 [PMID:29528226] Xu X, et al. Strigolactones positively regulate defense against root-knot nematodes in tomato. J. Exp. Bot., 2019. 70(4): p. 1325-1337 [PMID:30576511] Liu Y, et al. MYC2 Regulates the Termination of Jasmonate Signaling via an Autoregulatory Negative Feedback Loop. Plant Cell, 2019. 31(1): p. 106-127 [PMID:30610166]