PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID orange1.1g022760m
Common NameCISIN_1g013222mg
Organism
Citrus sinensis
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Sapindales; Rutaceae; Aurantioideae; Citrus
Family WRKY
Protein Properties Length: 293aa    MW: 32963.5 Da    PI: 5.2613
Description WRKY family protein
Gene Model
Gene Model ID Type Source Coding Sequence
orange1.1g022760mgenomeICGCView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1WRKY106.51.4e-33103160260
                        --SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS-- CS
               WRKY   2 dDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhek 60 
                        +DgynWrKYGqK+vkgse+prsY++Ct+++Cp+kkkvers  d++++ei+Y+g+Hnh+k
  orange1.1g022760m 103 EDGYNWRKYGQKQVKGSENPRSYFKCTFPDCPMKKKVERSL-DGQITEIVYKGSHNHPK 160
                        7****************************************.***************85 PP
2WRKY35.71.7e-11271292122
                        ---SS-EEEEEEE--TT-SS-E CS
               WRKY   1 ldDgynWrKYGqKevkgsefpr 22 
                        ldDgy+WrKYGqK+vkg+++pr
  orange1.1g022760m 271 LDDGYRWRKYGQKVVKGNPNPR 292
                        59*******************9 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
Gene3DG3DSA:2.20.25.802.0E-2894161IPR003657WRKY domain
PROSITE profilePS5081123.00997161IPR003657WRKY domain
SuperFamilySSF1182903.53E-2598161IPR003657WRKY domain
SMARTSM007742.7E-36102160IPR003657WRKY domain
PfamPF031062.0E-25103159IPR003657WRKY domain
Gene3DG3DSA:2.20.25.801.6E-13256292IPR003657WRKY domain
SuperFamilySSF1182901.83E-9263292IPR003657WRKY domain
PROSITE profilePS5081116.386266292IPR003657WRKY domain
PfamPF031065.6E-7272292IPR003657WRKY domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009409Biological Processresponse to cold
GO:0009414Biological Processresponse to water deprivation
GO:0009651Biological Processresponse to salt stress
GO:0010120Biological Processcamalexin biosynthetic process
GO:0010200Biological Processresponse to chitin
GO:0010508Biological Processpositive regulation of autophagy
GO:0042742Biological Processdefense response to bacterium
GO:0050832Biological Processdefense response to fungus
GO:0070370Biological Processcellular heat acclimation
GO:0005634Cellular Componentnucleus
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0043565Molecular Functionsequence-specific DNA binding
GO:0044212Molecular Functiontranscription regulatory region DNA binding
Sequence ? help Back to Top
Protein Sequence    Length: 293 aa     Download sequence    Send to blast
MEENNYFDFS FQTQTRPSSN SVSVQEESVK RQQEPWNFNK PTKESDFSTE NPGVKSEFAP  60
MESFSSDMAS YQTNVQSNAA PQSGNYGHYN QSSAYTREQK RSEDGYNWRK YGQKQVKGSE  120
NPRSYFKCTF PDCPMKKKVE RSLDGQITEI VYKGSHNHPK PTSTRRSSSS QSMQHSTCAN  180
SDLSDQSVGP LGNTHTDSFS MQNESSTSFG EDDFVEQGSP TSNPIGDDDE NEPDAKRWKG  240
ENDIEGVIGT GSRTVREPRI VVQTTSDIDI LDDGYRWRKY GQKVVKGNPN PR*
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
2ayd_A5e-161041611674WRKY transcription factor 1
Search in ModeBase
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: In dry seeds, expressed in aleurone cells and embryos. Levels drop rapidly but transiently in the embryos of imbibed seeds. {ECO:0000269|PubMed:19199048}.
UniprotTISSUE SPECIFICITY: Expressed in aleurone cells (PubMed:15618416). Mostly expressed in aleurone layers and leaves, and, to a lower extent, in roots, panicles and embryos (PubMed:26025535). {ECO:0000269|PubMed:15618416, ECO:0000269|PubMed:26025535}.
Functional Description ? help Back to Top
Source Description
UniProtTranscription activator (PubMed:26025535). Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (PubMed:19199048, PubMed:26025535). Negative regulator of both gibberellic acid (GA) and abscisic acid (ABA) signaling in aleurone cells, probably by interfering with GAM1, via the specific repression of GA- and ABA-induced promoters (PubMed:15618416, PubMed:19199048, PubMed:26025535). {ECO:0000269|PubMed:15618416, ECO:0000269|PubMed:19199048, ECO:0000269|PubMed:26025535}.
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00299DAPTransfer from AT2G38470Download
Motif logo
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Induced by abscisic acid (ABA) in aleurone cells, embryos, roots and leaves (PubMed:15618416, PubMed:19199048). Slightly down-regulated by gibberellic acid (GA) (PubMed:15618416). Accumulates in response to jasmonic acid (MeJA) (By similarity). {ECO:0000250|UniProtKB:Q6B6R4, ECO:0000269|PubMed:15618416, ECO:0000269|PubMed:19199048}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_006429505.10.0probable WRKY transcription factor 33 isoform X1
RefseqXP_006481125.10.0probable WRKY transcription factor 33 isoform X1
SwissprotQ6IEQ74e-81WRK24_ORYSJ; WRKY transcription factor WRKY24
TrEMBLA0A067FAY10.0A0A067FAY1_CITSI; Uncharacterized protein
STRINGXP_006481125.10.0(Citrus sinensis)
STRINGXP_006429505.10.0(Citrus clementina)
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT2G03340.11e-60WRKY DNA-binding protein 3
Publications ? help Back to Top
  1. Kikuchi S, et al.
    Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice.
    Science, 2003. 301(5631): p. 376-9
    [PMID:12869764]
  2. Zhang ZL, et al.
    A negative regulator encoded by a rice WRKY gene represses both abscisic acid and gibberellins signaling in aleurone cells.
    Plant Mol. Biol., 2009. 70(1-2): p. 139-51
    [PMID:19199048]
  3. Basu S,Roychoudhury A
    Expression profiling of abiotic stress-inducible genes in response to multiple stresses in rice (Oryza sativa L.) varieties with contrasting level of stress tolerance.
    Biomed Res Int, 2014. 2014: p. 706890
    [PMID:25110688]
  4. Zhang L, et al.
    Three WRKY transcription factors additively repress abscisic acid and gibberellin signaling in aleurone cells.
    Plant Sci., 2015. 236: p. 214-22
    [PMID:26025535]