PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

XP_013631647.1

Organism

Brassica oleracea

Taxonomic ID

3712

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica

Family

WRKY

Protein Properties

Length: 476aa MW: 52147.6 Da PI: 7.6548

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
XP_013631647.1	genome	NCBI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	103	1.7e-32	168	225	2	60
--SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS-- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhek 60 +DgynWrKYGqK+vkgse+prsYY+Ct+++Cp+kkkve+s +++++ei+Y+g+Hnh+k XP_013631647.1 168 EDGYNWRKYGQKQVKGSENPRSYYKCTFPNCPTKKKVETSL-EGQITEIVYKGSHNHPK 225 7**************************************.*************85 PP
2	WRKY	106.1	1.8e-33	326	384	1	59
---SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 1 ldDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhe 59 ldDgy+WrKYGqK+vkg+++prsYY+Ct++gCpv+k+ver+++d ++v++tYeg+Hnh+ XP_013631647.1 326 LDDGYRWRKYGQKVVKGNPNPRSYYKCTTNGCPVRKHVERASHDMRAVITTYEGKHNHD 384 59********************************************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	7.1E-27	160	226	IPR003657	WRKY domain
SuperFamily	SSF118290	3.27E-24	167	226	IPR003657	WRKY domain
SMART	SM00774	1.6E-33	167	225	IPR003657	WRKY domain
PROSITE profile	PS50811	22.171	168	226	IPR003657	WRKY domain
Pfam	PF03106	1.4E-24	168	224	IPR003657	WRKY domain
Gene3D	G3DSA:2.20.25.80	1.9E-37	311	386	IPR003657	WRKY domain
SuperFamily	SSF118290	2.48E-29	318	386	IPR003657	WRKY domain
PROSITE profile	PS50811	38.641	321	386	IPR003657	WRKY domain
SMART	SM00774	1.1E-38	326	385	IPR003657	WRKY domain
Pfam	PF03106	7.0E-26	327	384	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 476 aa Download sequence Send to blast
MAASSLLTMD NSITRQNMNG SANWSGIRSS AASLEDLEIP PKFRSFAPSS ISISPSLVSP 60 STCFSPSVFL DSPAFVASSA NVLASPTTGS LLTYENNQKS INEEEKTKKN NNNVNFFDFS 120 FQTQSSGVSA PTTTTTNSSV LQSQETRPNN NTHHQTASYN GREQRKGEDG YNWRKYGQKQ 180 VKGSENPRSY YKCTFPNCPT KKKVETSLEG QITEIVYKGS HNHPKPQSTR RSSSSSSTFH 240 SAVFDHHGDH SDSFVIQQDD NNTTSGSLGD DELSVMSRGE EEDCGSEPEA KRWKGESETN 300 GGNGNGSKTV REPRIVVQTT SDIDILDDGY RWRKYGQKVV KGNPNPRSYY KCTTNGCPVR 360 KHVERASHDM RAVITTYEGK HNHDVPAARG SGYTTNRLAQ DPSSAPIRPN AIAGHSSYTT 420 SSQAPYTLQM LHQNNNTSAG SFGYATNNNN FLGGGFSRAK EEPSDESSSF FDSFLS

Sequence ? help Back to Top

Protein Sequence Length: 476 aa Download sequence Send to blast

MAASSLLTMD NSITRQNMNG SANWSGIRSS AASLEDLEIP PKFRSFAPSS ISISPSLVSP  60
STCFSPSVFL DSPAFVASSA NVLASPTTGS LLTYENNQKS INEEEKTKKN NNNVNFFDFS  120
FQTQSSGVSA PTTTTTNSSV LQSQETRPNN NTHHQTASYN GREQRKGEDG YNWRKYGQKQ  180
VKGSENPRSY YKCTFPNCPT KKKVETSLEG QITEIVYKGS HNHPKPQSTR RSSSSSSTFH  240
SAVFDHHGDH SDSFVIQQDD NNTTSGSLGD DELSVMSRGE EEDCGSEPEA KRWKGESETN  300
GGNGNGSKTV REPRIVVQTT SDIDILDDGY RWRKYGQKVV KGNPNPRSYY KCTTNGCPVR  360
KHVERASHDM RAVITTYEGK HNHDVPAARG SGYTTNRLAQ DPSSAPIRPN AIAGHSSYTT  420
SSQAPYTLQM LHQNNNTSAG SFGYATNNNN FLGGGFSRAK EEPSDESSSF FDSFLS

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1wj2_A	1e-37	168	387	8	78	Probable WRKY transcription factor 4
2lex_A	1e-37	168	387	8	78	Probable WRKY transcription factor 4
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Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Highly expressed in roots, leaves and flowers, and at lower levels in stems, siliques and seeds. {ECO:0000269\|PubMed:18839316}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Interacts specifically with the W box (5'-TTGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Involved in defense responses. Required for resistance to the necrotrophic fungal pathogen B.cinerea (PubMed:17059405, PubMed:21990940). Regulates the antagonistic relationship between defense pathways mediating responses to the bacterial pathogen P. syringae and the necrotrophic pathogen B.cinerea (PubMed:17059405). Required for the phytoalexin camalexin synthesis following infection with B.cinerea. Acts as positive regulator of the camalexin biosynthetic genes PAD3 (CYP71B15) and CYP71A13 by binding to their promoters (PubMed:21498677, PubMed:22392279). Acts downstream of MPK3 and MPK6 in reprogramming the expression of camalexin biosynthetic genes, which drives the metabolic flow to camalexin production (PubMed:21498677). Functions with WRKY25 as positive regulator of salt stress response and abscisic acid (ABA) signaling (PubMed:18839316). Functions with WRKY25 and WRKY26 as positive regulator of plant thermotolerance by partially participating in ethylene-response signal transduction pathway (PubMed:21336597). The DNA-binding activity of WRKY33 is increased by SIB1 and SIB2 (PubMed:21990940). {ECO:0000269\|PubMed:18839316, ECO:0000269\|PubMed:21336597, ECO:0000269\|PubMed:21498677, ECO:0000269\|PubMed:21990940, ECO:0000269\|PubMed:22392279}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	XP_013631647.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By salt stress (PubMed:18839316). Induced by infection with the necrotrophic fungal pathogen B.cinerea (PubMed:17059405, PubMed:21498677, PubMed:21990940). Induced by infection with the bacterial pathogen P.syringae pv. tomato DC3000 (PubMed:17059405). {ECO:0000269\|PubMed:17059405, ECO:0000269\|PubMed:18839316, ECO:0000269\|PubMed:21498677, ECO:0000269\|PubMed:21990940}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	KM593145	0.0	KM593145.1 Brassica oleracea var. capitata WRKY transcription factor (WRKY45) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_013631647.1	0.0	PREDICTED: probable WRKY transcription factor 33
Swissprot	Q8S8P5	0.0	WRK33_ARATH; Probable WRKY transcription factor 33
TrEMBL	A0A0D3BQV0	0.0	A0A0D3BQV0_BRAOL; Uncharacterized protein
TrEMBL	A0A291B476	0.0	A0A291B476_BRANA; WRKY33
TrEMBL	A0A2P1D001	0.0	A0A2P1D001_BRAOT; Transcription factor WRKY33-2
STRING	Bo4g027280.1	0.0	(Brassica oleracea)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM4583	27	55

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT2G38470.1	0.0	WRKY DNA-binding protein 33

Publications ? help Back to Top
Brand LH,Kirchler T,Hummel S,Chaban C,Wanke D DPI-ELISA: a fast and versatile method to specify the binding of plant transcription factors to DNA in vitro. Plant Methods, 2010. 6: p. 25 [PMID:21108821] Brand LH, et al. Screening for protein-DNA interactions by automatable DNA-protein interaction ELISA. PLoS ONE, 2013. 8(10): p. e75177 [PMID:24146751] Ali MA,Wieczorek K,Kreil DP,Bohlmann H The beet cyst nematode Heterodera schachtii modulates the expression of WRKY transcription factors in syncytia to favour its development in Arabidopsis roots. PLoS ONE, 2014. 9(7): p. e102360 [PMID:25033038] Divi UK,Rahman T,Krishna P Gene expression and functional analyses in brassinosteroid-mediated stress tolerance. Plant Biotechnol. J., 2016. 14(1): p. 419-32 [PMID:25973891] Peskan-Berghöfer T, et al. Sustained exposure to abscisic acid enhances the colonization potential of the mutualist fungus Piriformospora indica on Arabidopsis thaliana roots. New Phytol., 2015. 208(3): p. 873-86 [PMID:26075497] Wang C, et al. The Arabidopsis Mediator Complex Subunit16 Is a Key Component of Basal Resistance against the Necrotrophic Fungal Pathogen Sclerotinia sclerotiorum. Plant Physiol., 2015. 169(1): p. 856-72 [PMID:26143252] Wang C, et al. Arabidopsis Elongator subunit 2 positively contributes to resistance to the necrotrophic fungal pathogens Botrytis cinerea and Alternaria brassicicola. Plant J., 2015. 83(6): p. 1019-33 [PMID:26216741] Datta R, et al. Glutathione Regulates 1-Aminocyclopropane-1-Carboxylate Synthase Transcription via WRKY33 and 1-Aminocyclopropane-1-Carboxylate Oxidase by Modulating Messenger RNA Stability to Induce Ethylene Synthesis during Stress. Plant Physiol., 2015. 169(4): p. 2963-81 [PMID:26463088] Daumann M,Fischer M,Niopek-Witz S,Girke C,Möhlmann T Apoplastic Nucleoside Accumulation in Arabidopsis Leads to Reduced Photosynthetic Performance and Increased Susceptibility Against Botrytis cinerea. Front Plant Sci, 2015. 6: p. 1158 [PMID:26779190] Liu S,Bartnikas LM,Volko SM,Ausubel FM,Tang D Mutation of the Glucosinolate Biosynthesis Enzyme Cytochrome P450 83A1 Monooxygenase Increases Camalexin Accumulation and Powdery Mildew Resistance. Front Plant Sci, 2016. 7: p. 227 [PMID:26973671] Jiang Y,Yu D The WRKY57 Transcription Factor Affects the Expression of Jasmonate ZIM-Domain Genes Transcriptionally to Compromise Botrytis cinerea Resistance. Plant Physiol., 2016. 171(4): p. 2771-82 [PMID:27268959] Liao CJ,Lai Z,Lee S,Yun DJ,Mengiste T Arabidopsis HOOKLESS1 Regulates Responses to Pathogens and Abscisic Acid through Interaction with MED18 and Acetylation of WRKY33 and ABI5 Chromatin. Plant Cell, 2016. 28(7): p. 1662-81 [PMID:27317674] Birkenbihl RP,Kracher B,Roccaro M,Somssich IE Induced Genome-Wide Binding of Three Arabidopsis WRKY Transcription Factors during Early MAMP-Triggered Immunity. Plant Cell, 2017. 29(1): p. 20-38 [PMID:28011690] Nguyen CC, et al. Overexpression of oligouridylate binding protein 1b results in ABA hypersensitivity. Plant Signal Behav, 2017. 12(2): p. e1282591 [PMID:28112571] Liu S,Ziegler J,Zeier J,Birkenbihl RP,Somssich IE Botrytis cinerea B05.10 promotes disease development in Arabidopsis by suppressing WRKY33-mediated host immunity. Plant Cell Environ., 2017. 40(10): p. 2189-2206 [PMID:28708934] D'Ambrosio JM, et al. Phospholipase C2 Affects MAMP-Triggered Immunity by Modulating ROS Production. Plant Physiol., 2017. 175(2): p. 970-981 [PMID:28827453] Liu F, et al. Interactions of WRKY15 and WRKY33 transcription factors and their roles in the resistance of oilseed rape to Sclerotinia infection. Plant Biotechnol. J., 2018. 16(4): p. 911-925 [PMID:28929638] Crespo-Salvador Ó,Escamilla-Aguilar M,López-Cruz J,López-Rodas G,González-Bosch C Determination of histone epigenetic marks in Arabidopsis and tomato genes in the early response to Botrytis cinerea. Plant Cell Rep., 2018. 37(1): p. 153-166 [PMID:29119291]