PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID XP_013610444.1
Organism
Brassica oleracea
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica
Family WRKY
Protein Properties Length: 321aa    MW: 36436.9 Da    PI: 8.855
Description WRKY family protein
Gene Model
Gene Model ID Type Source Coding Sequence
XP_013610444.1genomeNCBIView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1WRKY102.82e-32179236259
                     --SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS- CS
            WRKY   2 dDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhe 59 
                     +Dgy+WrKYGqK vk+s++prsYYrCt+++C+vkk+vers +dp+vv++tYe++H+h+
  XP_013610444.1 179 EDGYRWRKYGQKAVKNSPYPRSYYRCTTQKCNVKKRVERSYQDPTVVITTYESQHDHP 236
                     8********************************************************7 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PIRSFPIRSF0381303.0E-1011321IPR017396PWRKY transcription factor, group IIc
Gene3DG3DSA:2.20.25.804.4E-34163238IPR003657WRKY domain
SuperFamilySSF1182901.31E-28170238IPR003657WRKY domain
PROSITE profilePS5081131.823173238IPR003657WRKY domain
SMARTSM007745.3E-37178237IPR003657WRKY domain
PfamPF031067.9E-25179236IPR003657WRKY domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009737Biological Processresponse to abscisic acid
GO:0042742Biological Processdefense response to bacterium
GO:0050832Biological Processdefense response to fungus
GO:0051607Biological Processdefense response to virus
GO:0070301Biological Processcellular response to hydrogen peroxide
GO:1901002Biological Processpositive regulation of response to salt stress
GO:0005634Cellular Componentnucleus
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0043565Molecular Functionsequence-specific DNA binding
Sequence ? help Back to Top
Protein Sequence    Length: 321 aa     Download sequence    Send to blast
MSNETKDLNK YHYTSSYNHY NNINSQNMSL PYVSGPCTYN ANMISSQIGS DLHSSSQGAF  60
GFGFELSPSS SEFFNPSIDQ ENGFYNAYNY NTSLKSHEVV GGGGAIAENE TRVSASPSSG  120
EAHHHHGEDS GKSLLKREAD DGGDKQRSQK VVKTKKNQEK KIREPRVSFM TKTEVDHLED  180
GYRWRKYGQK AVKNSPYPRS YYRCTTQKCN VKKRVERSYQ DPTVVITTYE SQHDHPIPTT  240
RRTAMFSGPA ASDYKSSSLS PGSNFINTPR SFSHDDLFRV PYSSMNANPN YQQQQNHEFQ  300
HGSDYELLKE MFPSVFFKQK P
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
1wj2_A2e-27169238877Probable WRKY transcription factor 4
2lex_A2e-27169238877Probable WRKY transcription factor 4
Search in ModeBase
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Highly expressed in roots and at lower levels in rosette leaves, cauline leaves, stems, flowers and siliques. {ECO:0000269|PubMed:23451802}.
Functional Description ? help Back to Top
Source Description
UniProtTranscription factor. Interacts specifically with the W box (5'-TTGAC[CT]-3'), a frequently occurring stress-responsive cis-acting element. Functions as positive regulator of salt stress response. Binds the W box of LTI78/RD29A stress-response gene and directly regulates its transcription under salt stress. Functions antagonistically with VQ9 to regulate sodium and potassium homeostasis under salt stress by regulating the expression of downstream SOS (SALT OVERLY SENSITIVE) stress-responsive genes. The DNA-binding activity of WRKY8 is decreased by VQ9 (PubMed:23451802). Functions as negative regulator of basal resistance to the bacterial pathogen P. syringae and as positive regulator of resistance to the fungal pathogen to B. cinerea (PubMed:20367464). Functions as positive regulator of defense response againt tobamovirus (TMV) by regulating both the abscisic acid and ethylene signaling pathways. Positively regulates ABI4 expression and negatively modulates ACS6 and ERF104 expression by directly binding to the W box consensus motifs within their promoters (PubMed:23650359). {ECO:0000269|PubMed:20367464, ECO:0000269|PubMed:23451802, ECO:0000269|PubMed:23650359}.
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00545DAPTransfer from AT5G46350Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapXP_013610444.1
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: By wounding, abscisic acid (ABA), salicylic acid (SA), H(2)O(2), and infection with P.syringae pv. tomato DC3000 and B.cinerea (PubMed:20367464). Induced by salt stress (PubMed:23451802). {ECO:0000269|PubMed:20367464, ECO:0000269|PubMed:23451802}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankEU9124180.0EU912418.1 Brassica napus WRKY8-1 transcription factor (WRKY8-1) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_013610444.10.0PREDICTED: probable WRKY transcription factor 8
SwissprotQ9FL261e-161WRKY8_ARATH; WRKY transcription factor 8
TrEMBLA0A3P6DUA50.0A0A3P6DUA5_BRAOL; Uncharacterized protein
STRINGBra017561.1-P0.0(Brassica rapa)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM15242693
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT5G46350.11e-148WRKY DNA-binding protein 8
Publications ? help Back to Top
  1. Duarte JM, et al.
    Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis.
    Mol. Biol. Evol., 2006. 23(2): p. 469-78
    [PMID:16280546]
  2. Ding Y, et al.
    Four distinct types of dehydration stress memory genes in Arabidopsis thaliana.
    BMC Plant Biol., 2013. 13: p. 229
    [PMID:24377444]
  3. Sukweenadhi J, et al.
    Paenibacillus yonginensis DCY84(T) induces changes in Arabidopsis thaliana gene expression against aluminum, drought, and salt stress.
    Microbiol. Res., 2015. 172: p. 7-15
    [PMID:25721473]