PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

XP_013596685.1

Organism

Brassica oleracea

Taxonomic ID

3712

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica

Family

WRKY

Protein Properties

Length: 323aa MW: 36675.2 Da PI: 7.0331

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
XP_013596685.1	genome	NCBI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	104.2	7e-33	180	237	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhe 59 +Dgy+WrKYGqK vk+s++prsYYrCt+++C+vkk+vers +dp+vv++tYe++Hnh+ XP_013596685.1 180 EDGYRWRKYGQKAVKNSPYPRSYYRCTTQKCNVKKRVERSYQDPTVVITTYESQHNHP 237 8********************************************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PIRSF	PIRSF038130	4.0E-98	1	323	IPR017396	PWRKY transcription factor, group IIc
Gene3D	G3DSA:2.20.25.80	4.8E-34	164	239	IPR003657	WRKY domain
SuperFamily	SSF118290	6.93E-29	171	239	IPR003657	WRKY domain
PROSITE profile	PS50811	32.294	174	239	IPR003657	WRKY domain
SMART	SM00774	6.1E-38	179	238	IPR003657	WRKY domain
Pfam	PF03106	2.0E-25	180	237	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 323 aa Download sequence Send to blast
MSNETKDLNN YHFTSSYDHY NSINSQSIMN LAYLSGPSTY NANMISSQIG SDLHSCPRGA 60 CGLGFELSPS STEFFNSSID QENDFYNAYN YNTSHKSHEV VGGGGAIVES ETKVSASPSS 120 SEHHHGEDSG KSLMKREADD GGKDIDQRSQ KVVKTKKKEE KKQRESRVSF MTKTDVDHLE 180 DGYRWRKYGQ KAVKNSPYPR SYYRCTTQKC NVKKRVERSY QDPTVVITTY ESQHNHPIPT 240 SRRTAMFSGP AACSYNSSSL SPVSDFIINT PRTFSHDDLF RAPYASMNVN ANYEQQQSQE 300 FHHESDYELL KEMFPSVFLK QEP

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1wj2_A	1e-27	169	239	7	77	Probable WRKY transcription factor 4
2lex_A	1e-27	169	239	7	77	Probable WRKY transcription factor 4
Search in ModeBase

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Highly expressed in roots and at lower levels in rosette leaves, cauline leaves, stems, flowers and siliques. {ECO:0000269\|PubMed:23451802}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Interacts specifically with the W box (5'-TTGAC[CT]-3'), a frequently occurring stress-responsive cis-acting element. Functions as positive regulator of salt stress response. Binds the W box of LTI78/RD29A stress-response gene and directly regulates its transcription under salt stress. Functions antagonistically with VQ9 to regulate sodium and potassium homeostasis under salt stress by regulating the expression of downstream SOS (SALT OVERLY SENSITIVE) stress-responsive genes. The DNA-binding activity of WRKY8 is decreased by VQ9 (PubMed:23451802). Functions as negative regulator of basal resistance to the bacterial pathogen P. syringae and as positive regulator of resistance to the fungal pathogen to B. cinerea (PubMed:20367464). Functions as positive regulator of defense response againt tobamovirus (TMV) by regulating both the abscisic acid and ethylene signaling pathways. Positively regulates ABI4 expression and negatively modulates ACS6 and ERF104 expression by directly binding to the W box consensus motifs within their promoters (PubMed:23650359). {ECO:0000269\|PubMed:20367464, ECO:0000269\|PubMed:23451802, ECO:0000269\|PubMed:23650359}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	XP_013596685.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By wounding, abscisic acid (ABA), salicylic acid (SA), H(2)O(2), and infection with P.syringae pv. tomato DC3000 and B.cinerea (PubMed:20367464). Induced by salt stress (PubMed:23451802). {ECO:0000269\|PubMed:20367464, ECO:0000269\|PubMed:23451802}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	EU912418	0.0	EU912418.1 Brassica napus WRKY8-1 transcription factor (WRKY8-1) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_013596685.1	0.0	PREDICTED: probable WRKY transcription factor 8
Refseq	XP_013704057.1	0.0	probable WRKY transcription factor 8
Swissprot	Q9FL26	1e-160	WRKY8_ARATH; WRKY transcription factor 8
TrEMBL	A0A0D3D9H0	0.0	A0A0D3D9H0_BRAOL; WRKY transcription factor
STRING	Bo7g075300.1	0.0	(Brassica oleracea)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM1524	26	93

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT5G46350.1	1e-140	WRKY DNA-binding protein 8

Publications ? help Back to Top
Duarte JM, et al. Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. Mol. Biol. Evol., 2006. 23(2): p. 469-78 [PMID:16280546] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Sukweenadhi J, et al. Paenibacillus yonginensis DCY84(T) induces changes in Arabidopsis thaliana gene expression against aluminum, drought, and salt stress. Microbiol. Res., 2015. 172: p. 7-15 [PMID:25721473]