PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID GSBRNA2T00099024001
Common NameGSBRNA2T00099024001
Organism
Brassica napus
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica
Family MYB
Protein Properties Length: 305aa    MW: 34825.1 Da    PI: 5.7838
Description MYB family protein
Gene Model
Gene Model ID Type Source Coding Sequence
GSBRNA2T00099024001genomeGenoscopeView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1Myb_DNA-binding42.21.9e-131156146
                         TSSS-HHHHHHHHHHHHHTTTT-HHHHHHHHTTTS-HHHHHHHHHH CS
      Myb_DNA-binding  1 rgrWTteEdellvdavkqlGggtWktIartmgkgRtlkqcksrwqk 46
                         +g+W +eEde l++ v+++G+++W++I ++  + Rt+k+c++rw +
  GSBRNA2T00099024001 11 KGPWKAEEDEVLINHVNRYGPRDWSSIRSKGLLQRTGKSCRLRWVN 56
                         79*************************8887799**********87 PP
2Myb_DNA-binding42.81.2e-1371110344
                          SS-HHHHHHHHHHHHHTTTT-HHHHHHHHTTTS-HHHHHHHH CS
      Myb_DNA-binding   3 rWTteEdellvdavkqlGggtWktIartmgkgRtlkqcksrw 44 
                          ++++eE+   +++ +++G++ W++Ia +++ gRt++++k++w
  GSBRNA2T00099024001  71 KFSAEEERTVIELQAEFGNK-WARIATYLP-GRTDNDVKNFW 110
                          79******************.*********.*********** PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
Gene3DG3DSA:1.10.10.601.1E-21464IPR009057Homeodomain-like
PROSITE profilePS5129414.547658IPR017930Myb domain
SuperFamilySSF466891.02E-279110IPR009057Homeodomain-like
SMARTSM007171.0E-101060IPR001005SANT/Myb domain
PfamPF002491.8E-111156IPR001005SANT/Myb domain
CDDcd001678.77E-111358No hitNo description
PROSITE profilePS5129419.90859118IPR017930Myb domain
SMARTSM007171.4E-1268116IPR001005SANT/Myb domain
Gene3DG3DSA:1.10.10.607.6E-1771113IPR009057Homeodomain-like
CDDcd001671.09E-1071110No hitNo description
PfamPF002491.1E-1271110IPR001005SANT/Myb domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0003677Molecular FunctionDNA binding
Sequence ? help Back to Top
Protein Sequence    Length: 305 aa     Download sequence    Send to blast
MEAVKKEEMK KGPWKAEEDE VLINHVNRYG PRDWSSIRSK GLLQRTGKSC RLRWVNKLRP  60
NLKRCFFSGC KFSAEEERTV IELQAEFGNK WARIATYLPG RTDNDVKNFW SSRVKRLARI  120
LHNSSDASTS PKPPSRRNKG KNVKPIQCFY PPQFQSFGLV EEEVTTASSS SSKILPYSSS  180
DQVDEALRLS ESGIKLEHQP FSFGTDPLMS EPHIANQQAT IPFYPESREL LARLDNPYYY  240
DILGPAGCSE PLFALPQPFF ELPPAERRCR HVSKDDEEEE EADVFLDDFP ADMFDQVDDP  300
NQSP*
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
1h8a_C9e-28811624126MYB TRANSFORMING PROTEIN
Search in ModeBase
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: Strict spatial and temporal transcriptional activation leading to a specific expression in the male germline cells; this specific expression pattern is dependent of the regulatory region of DUO1 (ROD1) 5'-YAACYGY-3' in its promoter (PubMed:15694308, PubMed:27624837). First observed in the germ cell during or soon after engulfment by the vegetative cell, just after asymmetric division. In maturating pollen, accumulates in germ cells before mitosis and remains high in mature sperm cells. Expression persists during pollen development (PubMed:19300502). {ECO:0000269|PubMed:15694308, ECO:0000269|PubMed:19300502, ECO:0000269|PubMed:27624837}.
UniprotTISSUE SPECIFICITY: Confined to inflorescences, especially in stamens and pollen. {ECO:0000269|PubMed:15694308}.
Functional Description ? help Back to Top
Source Description
UniProtTranscription activator that acts as a positive regulator of male germline development by promoting both gametic cell specification and cell cycle progression (PubMed:15694308, PubMed:15618418, PubMed:19300502, PubMed:21285328). Binds to canonical MYB sites 5'-AACCGTC-3', 5'-AAACCGC-3' and 5'-AACCGT-3' in promoters to trigger the expression of male germline-specific or enriched genes (e.g. MGH3, GEX2 and GCS1), including those required for fertilization (PubMed:21285328, PubMed:19300502). Required for sperm cell specification leading to pollen maturation by activating a germline-specific regulon (PubMed:21285328, PubMed:15694308, PubMed:15618418, PubMed:19300502). Involved in pollen mitosis entry at G2-M transition via the regulation of CYCB1-1, DAZ1 and DAZ2 expression (PubMed:15618418, PubMed:19300502, PubMed:24876252). {ECO:0000269|PubMed:15618418, ECO:0000269|PubMed:15694308, ECO:0000269|PubMed:19300502, ECO:0000269|PubMed:21285328, ECO:0000269|PubMed:24876252}.
Cis-element ? help Back to Top
SourceLink
PlantRegMapGSBRNA2T00099024001
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Repressed by the microRNA 159 (miR159); the production of miR159 is stimulated by the anaphase promoting complex/cyclosome (APC/C) (PubMed:21441434). Activated by ARID1 in male germline cells via specific histone acetylation regulation (e.g. H3K9Ac) (PubMed:25057814). {ECO:0000269|PubMed:21441434, ECO:0000269|PubMed:25057814}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_022572064.10.0transcription factor DUO1-like
SwissprotA0A178VEK71e-149DUO1_ARATH; Transcription factor DUO1
TrEMBLA0A3P6BBG00.0A0A3P6BBG0_BRACM; Uncharacterized protein
STRINGBo6g077030.10.0(Brassica oleracea)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM65512842
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT3G60460.11e-145MYB family protein
Publications ? help Back to Top
  1. Zheng B,He H,Zheng Y,Wu W,McCormick S
    An ARID domain-containing protein within nuclear bodies is required for sperm cell formation in Arabidopsis thaliana.
    PLoS Genet., 2014. 10(7): p. e1004421
    [PMID:25057814]
  2. Chalhoub B, et al.
    Plant genetics. Early allopolyploid evolution in the post-Neolithic Brassica napus oilseed genome.
    Science, 2014. 345(6199): p. 950-3
    [PMID:25146293]
  3. Peters B, et al.
    A Conserved cis-Regulatory Module Determines Germline Fate through Activation of the Transcription Factor DUO1 Promoter.
    Plant Physiol., 2017. 173(1): p. 280-293
    [PMID:27624837]
  4. Zheng Z, et al.
    Target RNA Secondary Structure Is a Major Determinant of miR159 Efficacy.
    Plant Physiol., 2017. 174(3): p. 1764-1778
    [PMID:28515145]