PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT5G67300.1
Common NameATMYB44, ATMYBR1, K8K14.2, MYB44, MYBR1
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family MYB
Protein Properties Length: 305aa    MW: 33268.2 Da    PI: 8.1123
Description myb domain protein r1
Gene Model
Gene Model ID Type Source Coding Sequence
AT5G67300.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
  Myb_DNA-binding  1 rgrWTteEdellvdavkqlGggtWktIartmgkgRtlkqcksrwqky 47
                     +g+W++eEde+l ++v ++G+++W+ I++ ++ gR++k+c++rw + 
                     79******************************.***********985 PP

  Myb_DNA-binding   3 rWTteEdellvdavkqlGggtWktIartmgkgRtlkqcksrwqky 47 
                      ++++eEde +++a++q+G++ W+tIar ++ gRt++ +k++w++ 
                      89******************.*********.***********986 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PROSITE profilePS5129429.606156IPR017930Myb domain
SMARTSM007171.6E-17554IPR001005SANT/Myb domain
PfamPF002498.8E-19651IPR001005SANT/Myb domain
CDDcd001677.72E-17850No hitNo description
SMARTSM007171.3E-1457105IPR001005SANT/Myb domain
PROSITE profilePS5129419.7758107IPR017930Myb domain
CDDcd001678.77E-1260103No hitNo description
PfamPF002497.0E-1560102IPR001005SANT/Myb domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006357Biological Processregulation of transcription from RNA polymerase II promoter
GO:0009414Biological Processresponse to water deprivation
GO:0009651Biological Processresponse to salt stress
GO:0009723Biological Processresponse to ethylene
GO:0009733Biological Processresponse to auxin
GO:0009737Biological Processresponse to abscisic acid
GO:0009738Biological Processabscisic acid-activated signaling pathway
GO:0009739Biological Processresponse to gibberellin
GO:0010200Biological Processresponse to chitin
GO:0030154Biological Processcell differentiation
GO:0042742Biological Processdefense response to bacterium
GO:0046686Biological Processresponse to cadmium ion
GO:0050832Biological Processdefense response to fungus
GO:2000022Biological Processregulation of jasmonic acid mediated signaling pathway
GO:2000031Biological Processregulation of salicylic acid mediated signaling pathway
GO:0005634Cellular Componentnucleus
GO:0000981Molecular FunctionRNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0001135Molecular Functiontranscription factor activity, RNA polymerase II transcription factor recruiting
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0043565Molecular Functionsequence-specific DNA binding
GO:0044212Molecular Functiontranscription regulatory region DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000034anatomyvascular system
PO:0000037anatomyshoot apex
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009025anatomyvascular leaf
PO:0009052anatomyflower pedicel
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 305 aa     Download sequence    Send to blast
3D Structure ? help Back to Top
PDB ID Evalue Query Start Query End Hit Start Hit End Description
1mse_C2e-3951073105C-Myb DNA-Binding Domain
1msf_C2e-3951073105C-Myb DNA-Binding Domain
Search in ModeBase
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.288160.0bud| flower| leaf| root| seed
Expression -- Microarray ? help Back to Top
Source ID E-value
Expression AtlasAT5G67300-
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: Expressed during very late stages of embryogenesis. Later, its expression follows a development dependent gradient in successive leaves. {ECO:0000269|PubMed:9678577}.
UniprotTISSUE SPECIFICITY: Expressed in roots, stems, leaves, inflorescence, and flowers (including stamen, floral nectar, carpel, petal and sepal), mostly in vasculatures and stomata. {ECO:0000269|PubMed:18162593, ECO:0000269|PubMed:9678577}.
Functional Description ? help Back to Top
Source Description
TAIRMember of the R2R3 factor MYB gene family involved in mediating plant responses to a variety of abiotic stimiuli.
UniProtTranscription factor (PubMed:23067202, PubMed:23603962). Represses the expression of protein phosphatases 2C in response to abscisic acid (ABA). Confers resistance to abiotic stresses dependent of ABA (PubMed:18162593, PubMed:9678577). In response to auxin, activates the transcription of the auxin-responsive gene IAA19. The IAA19 transcription activation by MYB44 is enhanced by direct interaction between MYB44 and PYL8 (PubMed:24894996). Transcriptional activator of WRKY70 by direct binding to its promoter region, especially at 5'-TAACNG-3' and 5'-CNGTTA-3' symmetric motifs (PubMed:23067202, PubMed:23603962). Activates salicylic acid (SA)- mediated defenses and subsequent resistance to biotrophic pathogen P.syringae pv. tomato DC3000, but represses jasmonic acid (JA)-mediated defenses responses against the necrotrophic pathogen A.brassicicola (PubMed:23067202). {ECO:0000269|PubMed:18162593, ECO:0000269|PubMed:23067202, ECO:0000269|PubMed:23603962, ECO:0000269|PubMed:24894996, ECO:0000269|PubMed:9678577}.
Function -- GeneRIF ? help Back to Top
  1. Enhanced abiotic stress tolerance of transgenic Arabidopsis overexpressing AtMYB44 was conferred by reduced expression of genes encoding a group of serine/threonine protein phosphatases 2C.
    [PMID: 18162593]
  2. AtMYB44 gene activation appears to not be induced by any specific hormone
    [PMID: 20016937]
  3. AtMYB44 regulates induced expression of EIN2 in HrpN(Ea)-treated Arabidopsis plants. AtMYB44 protein localizes to nuclei and binds the EIN2 promoter; HrpN(Ea) treatment promotes AtMYB44 production, binding activity, and transcription of AtMYB44 and EIN2.
    [PMID: 21117868]
  4. HrpN Ea-induced deterrent effect on phloem feeding of the green peach aphid Myzus persicae requires AtGSL5 and AtMYB44 genes in Arabidopsis thaliana.
    [PMID: 21451254]
  5. the transcriptional regulator MYB44 has a role in the control of seed germination in Arabidopsis thaliana.
    [PMID: 22704933]
  6. AtMYB44 modulates antagonistic interaction by activating SA-mediated defenses and repressing JA-mediated defenses through direct control of WRKY70
    [PMID: 23067202]
  7. the C-terminal side of the protein, but not the N-terminal side of the R2R3 domain, contributes to the functional activity and specificity of AtMYB44 through interactions with other regulators generated by each of a variety of stimuli.
    [PMID: 23161171]
  8. MYB44 is regulated by MPK3 via phosphorylation whereby initiating the adaptation response to numerous abiotic and biotic stresses.
    [PMID: 23437396]
  9. AtMYB44 is an integrator of cross-talk between salicylic acid and jasmonic acid in plant defense responses.
    [PMID: 23603962]
  10. Dominant repression by Arabidopsis transcription factor MYB44 causes oxidative damage and hypersensitivity to abiotic stress.
    [PMID: 24531138]
  11. The authors herein revealed that the MYB44-mediated prevention of Pseudomonas syringae penetration through the stomata is one of the components responsible for Penicillium simplicissimum GP17-2-elicited induced systemic resistance.
    [PMID: 27301421]
  12. AtMYB44 might participate in the 3OC6-HSL-mediated primary root growth via regulating the expression of cytokinin- and auxin-related genes. The data establish a genetic connection between the regulatory role of AtMYB44 in phytohormones-related gene expression and plant response to the bacterial QS signal.
    [PMID: 27604593]
  13. salt stress induces the eviction of H2A.Z-containing nucleosomes from the AtMYB44 promoter region.
    [PMID: 29649476]
  14. AtMYB44 forms a complex with TPR corepressors and recruits histone deacetylase(s) to suppress PP2C gene transcription in a signal-independent manner.
    [PMID: 30448055]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
Motif logo
Cis-element ? help Back to Top
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: By drought, cold, high salinity, cadmium (CdCl(2)), salicylic acid (SA), jasmonate (JA), ethylene, gibberellic acid (GA), and ABA (PubMed:16463103, PubMed:18162593, PubMed:23067202). The induction by JA is COI1-dependent (PubMed:23067202). {ECO:0000269|PubMed:16463103, ECO:0000269|PubMed:18162593, ECO:0000269|PubMed:23067202}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
Regulation -- ATRM (Manually Curated Upstream Regulators) ? help Back to Top
Source Upstream Regulator (A: Activate/R: Repress)
ATRM AT3G26790 (A)
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT1G17550(R), AT1G72770(R), AT3G11410(R), AT4G26080(R), AT5G03280(A), AT5G44420(R), AT5G57050(R)
Regulation -- Hormone ? help Back to Top
Source Hormone
AHDabscisic acid, auxin, ethylene, gibberellin, jasmonic acid, Jasmonic acid, salicylic acid
Interaction ? help Back to Top
Source Intact With
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT5G67300
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAB0076450.0AB007645.1 Arabidopsis thaliana genomic DNA, chromosome 5, TAC clone:K8K14.
GenBankAF3268770.0AF326877.1 Arabidopsis thaliana putative myb-related protein, 33.3K (At5g67300) mRNA, complete cds.
GenBankAF3396980.0AF339698.1 Arabidopsis thaliana putative myb-related protein, 33.3K (At5g67300) mRNA, complete cds.
GenBankAY5196480.0AY519648.1 Arabidopsis thaliana MYB transcription factor (At5g67300) mRNA, complete cds.
GenBankCP0026880.0CP002688.1 Arabidopsis thaliana chromosome 5 sequence.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_201531.10.0myb domain protein r1
SwissprotQ9FDW10.0MYB44_ARATH; Transcription factor MYB44
STRINGAT5G67300.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Representative plantOGRP5171784
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
  2. Stracke R,Werber M,Weisshaar B
    The R2R3-MYB gene family in Arabidopsis thaliana.
    Curr. Opin. Plant Biol., 2001. 4(5): p. 447-56
  3. Kersten B, et al.
    Generation of Arabidopsis protein chips for antibody and serum screening.
    Plant Mol. Biol., 2003. 52(5): p. 999-1010
  4. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
  5. Leonhardt N, et al.
    Microarray expression analyses of Arabidopsis guard cells and isolation of a recessive abscisic acid hypersensitive protein phosphatase 2C mutant.
    Plant Cell, 2004. 16(3): p. 596-615
  6. Ko JH,Han KH,Park S,Yang J
    Plant body weight-induced secondary growth in Arabidopsis and its transcription phenotype revealed by whole-transcriptome profiling.
    Plant Physiol., 2004. 135(2): p. 1069-83
  7. Reymond P, et al.
    A conserved transcript pattern in response to a specialist and a generalist herbivore.
    Plant Cell, 2004. 16(11): p. 3132-47
  8. Devoto A, et al.
    Expression profiling reveals COI1 to be a key regulator of genes involved in wound- and methyl jasmonate-induced secondary metabolism, defence, and hormone interactions.
    Plant Mol. Biol., 2005. 58(4): p. 497-513
  9. Lee BH,Henderson DA,Zhu JK
    The Arabidopsis cold-responsive transcriptome and its regulation by ICE1.
    Plant Cell, 2005. 17(11): p. 3155-75
  10. Yanhui C, et al.
    The MYB transcription factor superfamily of Arabidopsis: expression analysis and phylogenetic comparison with the rice MYB family.
    Plant Mol. Biol., 2006. 60(1): p. 107-24
  11. Ma S,Bohnert HJ
    Integration of Arabidopsis thaliana stress-related transcript profiles, promoter structures, and cell-specific expression.
    Genome Biol., 2007. 8(4): p. R49
  12. Shin R, et al.
    The Arabidopsis transcription factor MYB77 modulates auxin signal transduction.
    Plant Cell, 2007. 19(8): p. 2440-53
  13. Libault M,Wan J,Czechowski T,Udvardi M,Stacey G
    Identification of 118 Arabidopsis transcription factor and 30 ubiquitin-ligase genes responding to chitin, a plant-defense elicitor.
    Mol. Plant Microbe Interact., 2007. 20(8): p. 900-11
  14. Wang Z, et al.
    Identification and characterization of COI1-dependent transcription factor genes involved in JA-mediated response to wounding in Arabidopsis plants.
    Plant Cell Rep., 2008. 27(1): p. 125-35
  15. Jung C, et al.
    Overexpression of AtMYB44 enhances stomatal closure to confer abiotic stress tolerance in transgenic Arabidopsis.
    Plant Physiol., 2008. 146(2): p. 623-35
  16. Sreenivasulu N, et al.
    Barley grain maturation and germination: metabolic pathway and regulatory network commonalities and differences highlighted by new MapMan/PageMan profiling tools.
    Plant Physiol., 2008. 146(4): p. 1738-58
  17. Huang D,Wu W,Abrams SR,Cutler AJ
    The relationship of drought-related gene expression in Arabidopsis thaliana to hormonal and environmental factors.
    J. Exp. Bot., 2008. 59(11): p. 2991-3007
  18. Pitzschke A,Djamei A,Teige M,Hirt H
    VIP1 response elements mediate mitogen-activated protein kinase 3-induced stress gene expression.
    Proc. Natl. Acad. Sci. U.S.A., 2009. 106(43): p. 18414-9
  19. Jung C, et al.
    Non-specific phytohormonal induction of AtMYB44 and suppression of jasmonate-responsive gene activation in Arabidopsis thaliana.
    Mol. Cells, 2010. 29(1): p. 71-6
  20. Liu R, et al.
    Thirty-seven transcription factor genes differentially respond to a harpin protein and affect resistance to the green peach aphid in Arabidopsis.
    J. Biosci., 2010. 35(3): p. 435-50
  21. Liu R, et al.
    Transcription factor AtMYB44 regulates induced expression of the ETHYLENE INSENSITIVE2 gene in Arabidopsis responding to a harpin protein.
    Mol. Plant Microbe Interact., 2011. 24(3): p. 377-89
  22. L
    HrpN Ea-induced deterrent effect on phloem feeding of the green peach aphid Myzus persicae requires AtGSL5 and AtMYB44 genes in Arabidopsis thaliana.
    J. Biosci., 2011. 36(1): p. 123-37
  23. Causier B,Ashworth M,Guo W,Davies B
    The TOPLESS interactome: a framework for gene repression in Arabidopsis.
    Plant Physiol., 2012. 158(1): p. 423-38
  24. Nguyen XC, et al.
    Phosphorylation of the transcriptional regulator MYB44 by mitogen activated protein kinase regulates Arabidopsis seed germination.
    Biochem. Biophys. Res. Commun., 2012. 423(4): p. 703-8
  25. Shim JS, et al.
    AtMYB44 regulates WRKY70 expression and modulates antagonistic interaction between salicylic acid and jasmonic acid signaling.
    Plant J., 2013. 73(3): p. 483-95
  26. Jung C, et al.
    Quadruple 9-mer-based protein binding microarray analysis confirms AACnG as the consensus nucleotide sequence sufficient for the specific binding of AtMYB44.
    Mol. Cells, 2012. 34(6): p. 531-7
  27. Persak H,Pitzschke A
    Tight interconnection and multi-level control of Arabidopsis MYB44 in MAPK cascade signalling.
    PLoS ONE, 2013. 8(2): p. e57547
  28. Shim JS,Choi YD
    Direct regulation of WRKY70 by AtMYB44 in plant defense responses.
    Plant Signal Behav, 2013. 8(6): p. e20783
  29. L
    AtMYB44 regulates resistance to the green peach aphid and diamondback moth by activating EIN2-affected defences in Arabidopsis.
    Plant Biol (Stuttg), 2013. 15(5): p. 841-50
  30. Li C,Chang PP,Ghebremariam KM,Qin L,Liang Y
    Overexpression of tomato SpMPK3 gene in Arabidopsis enhances the osmotic tolerance.
    Biochem. Biophys. Res. Commun., 2014. 443(2): p. 357-62
  31. Jaradat MR,Feurtado JA,Huang D,Lu Y,Cutler AJ
    Multiple roles of the transcription factor AtMYBR1/AtMYB44 in ABA signaling, stress responses, and leaf senescence.
    BMC Plant Biol., 2013. 13: p. 192
  32. Ding Y, et al.
    Four distinct types of dehydration stress memory genes in Arabidopsis thaliana.
    BMC Plant Biol., 2013. 13: p. 229
  33. Persak H,Pitzschke A
    Dominant repression by Arabidopsis transcription factor MYB44 causes oxidative damage and hypersensitivity to abiotic stress.
    Int J Mol Sci, 2014. 15(2): p. 2517-37
  34. Li D, et al.
    Arabidopsis ABA receptor RCAR1/PYL9 interacts with an R2R3-type MYB transcription factor, AtMYB44.
    Int J Mol Sci, 2014. 15(5): p. 8473-90
  35. Zhao Y, et al.
    The ABA receptor PYL8 promotes lateral root growth by enhancing MYB77-dependent transcription of auxin-responsive genes.
    Sci Signal, 2014. 7(328): p. ra53
  36. Xu DB, et al.
    A G-protein β subunit, AGB1, negatively regulates the ABA response and drought tolerance by down-regulating AtMPK6-related pathway in Arabidopsis.
    PLoS ONE, 2015. 10(1): p. e0116385
  37. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
  38. Hieno A, et al.
    Possible Involvement of MYB44-Mediated Stomatal Regulation in Systemic Resistance Induced by Penicillium simplicissimum GP17-2 in Arabidopsis.
    Microbes Environ., 2016. 31(2): p. 154-9
  39. Zhao Q, et al.
    AtMYB44 Positively Regulates the Enhanced Elongation of Primary Roots Induced by N-3-Oxo-Hexanoyl-Homoserine Lactone in Arabidopsis thaliana.
    Mol. Plant Microbe Interact., 2016. 29(10): p. 774-785
  40. Song L, et al.
    A transcription factor hierarchy defines an environmental stress response network.
    Science, 2017.
  41. Nguyen NH,Cheong JJ
    H2A.Z-containing nucleosomes are evicted to activate AtMYB44 transcription in response to salt stress.
    Biochem. Biophys. Res. Commun., 2018. 499(4): p. 1039-1043
  42. Nguyen NH,Cheong JJ
    AtMYB44 interacts with TOPLESS-RELATED corepressors to suppress protein phosphatase 2C gene transcription.
    Biochem. Biophys. Res. Commun., 2018. 507(1-4): p. 437-442
  43. Kirik V,K
    Two novel MYB homologues with changed expression in late embryogenesis-defective Arabidopsis mutants.
    Plant Mol. Biol., 1998. 37(5): p. 819-27