PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT5G47220.1

Common Name

ATERF2, ATERF-2, ERF101, ERF2, ERF-2, MQL5.7

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

ERF

Protein Properties

Length: 243aa MW: 26797 Da PI: 5.4801

Description

ethylene responsive element binding factor 2

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT5G47220.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	AP2	62.4	1e-19	116	166	2	55
AP2 2 gykGVrwdkkrgrWvAeIrdpsengkrkrfslgkfgtaeeAakaaiaarkkleg 55 +y+GVr+++ +g+++AeIrdp +ng +r++lg+f tae+Aa a++ a+ +++g AT5G47220.1 116 HYRGVRQRP-WGKFAAEIRDPAKNG--ARVWLGTFETAEDAALAYDIAAFRMRG 166 8******.*******9987..***********************98 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
CDD	cd00018	1.50E-32	115	174	No hit	No description
Gene3D	G3DSA:3.30.730.10	2.8E-32	115	174	IPR001471	AP2/ERF domain
Pfam	PF00847	2.2E-13	116	166	IPR001471	AP2/ERF domain
PROSITE profile	PS51032	24.54	116	174	IPR001471	AP2/ERF domain
SMART	SM00380	5.3E-41	116	180	IPR001471	AP2/ERF domain
SuperFamily	SSF54171	2.81E-23	116	176	IPR016177	DNA-binding domain
PRINTS	PR00367	4.5E-11	117	128	IPR001471	AP2/ERF domain
PRINTS	PR00367	4.5E-11	140	156	IPR001471	AP2/ERF domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0001944	Biological Process	vasculature development
GO:0009864	Biological Process	induced systemic resistance, jasmonic acid mediated signaling pathway
GO:0009873	Biological Process	ethylene-activated signaling pathway
GO:0010200	Biological Process	response to chitin
GO:0045893	Biological Process	positive regulation of transcription, DNA-templated
GO:0051301	Biological Process	cell division
GO:0005634	Cellular Component	nucleus
GO:0003677	Molecular Function	DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000013	anatomy	cauline leaf
PO:0000037	anatomy	shoot apex
PO:0000230	anatomy	inflorescence meristem
PO:0000293	anatomy	guard cell
PO:0008019	anatomy	leaf lamina base
PO:0009005	anatomy	root
PO:0009006	anatomy	shoot system
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009025	anatomy	vascular leaf
PO:0009029	anatomy	stamen
PO:0009030	anatomy	carpel
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0009052	anatomy	flower pedicel
PO:0020030	anatomy	cotyledon
PO:0020038	anatomy	petiole
PO:0020100	anatomy	hypocotyl
PO:0020137	anatomy	leaf apex
PO:0025022	anatomy	collective leaf structure
PO:0025281	anatomy	pollen
PO:0001054	developmental stage	vascular leaf senescent stage
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0001081	developmental stage	mature plant embryo stage
PO:0001185	developmental stage	plant embryo globular stage
PO:0004507	developmental stage	plant embryo bilateral stage
PO:0007064	developmental stage	LP.12 twelve leaves visible stage
PO:0007095	developmental stage	LP.08 eight leaves visible stage
PO:0007098	developmental stage	LP.02 two leaves visible stage
PO:0007103	developmental stage	LP.10 ten leaves visible stage
PO:0007115	developmental stage	LP.04 four leaves visible stage
PO:0007123	developmental stage	LP.06 six leaves visible stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 243 aa Download sequence Send to blast
MYGQCNIESD YALLESITRH LLGGGGENEL RLNESTPSSC FTESWGGLPL KENDSEDMLV 60 YGLLKDAFHF DTSSSDLSCL FDFPAVKVEP TENFTAMEEK PKKAIPVTET AVKAKHYRGV 120 RQRPWGKFAA EIRDPAKNGA RVWLGTFETA EDAALAYDIA AFRMRGSRAL LNFPLRVNSG 180 EPDPVRITSK RSSSSSSSSS SSTSSSENGK LKRRRKAENL TSEVVQVKCE VGDETRVDEL 240 LVS

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
2gcc_A	5e-43	112	181	1	70	ATERF1
3gcc_A	5e-43	112	181	1	70	ATERF1
Search in ModeBase

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.19731	0.0	floral meristem\| root

Expression -- Microarray ? help Back to Top
Source	ID	E-value
Genevisible	248794_at	0.0
Expression Atlas	AT5G47220	-
AtGenExpress	AT5G47220	-
ATTED-II	AT5G47220	-

Functional Description ? help Back to Top
Source	Description
TAIR	Encodes a member of the ERF (ethylene response factor) subfamily B-3 of ERF/AP2 transcription factor family (ATERF-2). The protein contains one AP2 domain. Functions as activator of GCC boxdependent transcription. Positive regulator of JA-responsive defense genes and resistance to F. oxysporum and enhances JA inhibition of root elongation.
UniProt	Acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. Involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways. Involved in disease resistance pathways. {ECO:0000269\|PubMed:10715325, ECO:0000269\|PubMed:12805630, ECO:0000269\|PubMed:9756931}.

Function -- GeneRIF ? help Back to Top
Expression of several ethylene biosynthetic genes and ethylene-responsive factors, including ERF1 and ERF2, was induced during reoxygenation. [PMID: 24506560]

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00548	DAP	27203113	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT5G47220.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Induced by jasmonate (JA) and Alternaria brassicicola (locally and systemically). Ethylene induction is completely dependent on a functional ETHYLENE-INSENSITIVE2 (EIN2) while wounding induction does not require EIN2. Transcripts accumulate strongly in cycloheximide-treated plants, a protein synthesis inhibitor. Seems to not be influenced by exogenous abscisic acid (ABA), cold, heat, NaCl or drought stress. {ECO:0000269\|PubMed:10715325, ECO:0000269\|PubMed:12805630}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Regulation -- ATRM (Manually Curated Upstream Regulators) ? help Back to Top
Source	Upstream Regulator (A: Activate/R: Repress)
ATRM	AT1G32640 (R), AT3G20770 (A), AT5G15850 (A)

Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source	Target Gene (A: Activate/R: Repress)
ATRM	AT1G06160(A), AT1G72260(A), AT3G04720(A), AT3G55730(A), AT5G44420(A)

Regulation -- Hormone ? help Back to Top
Source	Hormone
AHD	ethylene

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT5G47220

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AB008104	0.0	AB008104.1 Arabidopsis thaliana AtERF-2 mRNA for ethylene responsive element binding factor 2, complete cds.
GenBank	AB018117	0.0	AB018117.1 Arabidopsis thaliana genomic DNA, chromosome 5, P1 clone:MQL5.
GenBank	AK175151	0.0	AK175151.1 Arabidopsis thaliana mRNA for ethylene responsive element binding factor 2 (ATERF2), complete cds, clone: RAFL21-57-E18.
GenBank	AK175229	0.0	AK175229.1 Arabidopsis thaliana mRNA for ethylene responsive element binding factor 2, complete cds, clone: RAFL21-65-J18.
GenBank	AK176532	0.0	AK176532.1 Arabidopsis thaliana mRNA for ethylene responsive element binding factor 2 (ATERF2), complete cds, clone: RAFL25-05-B19.
GenBank	AK176606	0.0	AK176606.1 Arabidopsis thaliana mRNA for ethylene responsive element binding factor 2 (ATERF2), complete cds, clone: RAFL25-13-E16.
GenBank	AK176825	0.0	AK176825.1 Arabidopsis thaliana mRNA for ethylene responsive element binding factor 2 (ATERF2), complete cds, clone: RAFL25-38-F08.
GenBank	BT024470	0.0	BT024470.1 Arabidopsis thaliana At5g47220 mRNA, complete cds.
GenBank	CP002688	0.0	CP002688.1 Arabidopsis thaliana chromosome 5 sequence.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_199533.1	1e-180	ethylene responsive element binding factor 2
Swissprot	O80338	0.0	EF101_ARATH; Ethylene-responsive transcription factor 2
TrEMBL	A0A178UCP3	1e-179	A0A178UCP3_ARATH; ERF2
STRING	AT5G47220.1	1e-179	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM10	28	1650
Representative plant	OGRP6	16	1718

Link Out ? help Back to Top
Phytozome	AT5G47220.1
Entrez Gene	834768
iHOP	AT5G47220
wikigenes	AT5G47220

Publications ? help Back to Top
Fujimoto SY,Ohta M,Usui A,Shinshi H,Ohme-Takagi M Arabidopsis ethylene-responsive element binding factors act as transcriptional activators or repressors of GCC box-mediated gene expression. Plant Cell, 2000. 12(3): p. 393-404 [PMID:10715325] Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] O Identification of Arabidopsis ethylene-responsive element binding factors with distinct induction kinetics after pathogen infection. Plant Physiol., 2002. 128(4): p. 1313-22 [PMID:11950980] Brown RL,Kazan K,McGrath KC,Maclean DJ,Manners JM A role for the GCC-box in jasmonate-mediated activation of the PDF1.2 gene of Arabidopsis. Plant Physiol., 2003. 132(2): p. 1020-32 [PMID:12805630] McGrath KC, et al. Repressor- and activator-type ethylene response factors functioning in jasmonate signaling and disease resistance identified via a genome-wide screen of Arabidopsis transcription factor gene expression. Plant Physiol., 2005. 139(2): p. 949-59 [PMID:16183832] Duarte JM, et al. Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. Mol. Biol. Evol., 2006. 23(2): p. 469-78 [PMID:16280546] Nakano T,Suzuki K,Fujimura T,Shinshi H Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiol., 2006. 140(2): p. 411-32 [PMID:16407444] Dombrecht B, et al. MYC2 differentially modulates diverse jasmonate-dependent functions in Arabidopsis. Plant Cell, 2007. 19(7): p. 2225-45 [PMID:17616737] Libault M,Wan J,Czechowski T,Udvardi M,Stacey G Identification of 118 Arabidopsis transcription factor and 30 ubiquitin-ligase genes responding to chitin, a plant-defense elicitor. Mol. Plant Microbe Interact., 2007. 20(8): p. 900-11 [PMID:17722694] Ascencio-Ib Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection. Plant Physiol., 2008. 148(1): p. 436-54 [PMID:18650403] Gong W, et al. The development of protein microarrays and their applications in DNA-protein and protein-protein interaction analyses of Arabidopsis transcription factors. Mol Plant, 2008. 1(1): p. 27-41 [PMID:19802365] Kouno T,Ezaki B Multiple regulation of Arabidopsis AtGST11 gene expression by four transcription factors under abiotic stresses. Physiol Plant, 2013. 148(1): p. 97-104 [PMID:23009097] Etchells JP,Provost CM,Turner SR Plant vascular cell division is maintained by an interaction between PXY and ethylene signalling. PLoS Genet., 2012. 8(11): p. e1002997 [PMID:23166504] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Tsai KJ,Chou SJ,Shih MC Ethylene plays an essential role in the recovery of Arabidopsis during post-anaerobiosis reoxygenation. Plant Cell Environ., 2014. 37(10): p. 2391-405 [PMID:24506560] Lumba S, et al. A mesoscale abscisic acid hormone interactome reveals a dynamic signaling landscape in Arabidopsis. Dev. Cell, 2014. 29(3): p. 360-72 [PMID:24823379] Jin J, et al. An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors. Mol. Biol. Evol., 2015. 32(7): p. 1767-73 [PMID:25750178] Riechmann JL,Meyerowitz EM The AP2/EREBP family of plant transcription factors. Biol. Chem., 1998. 379(6): p. 633-46 [PMID:9687012] Hao D,Ohme-Takagi M,Sarai A Unique mode of GCC box recognition by the DNA-binding domain of ethylene-responsive element-binding factor (ERF domain) in plant. J. Biol. Chem., 1998. 273(41): p. 26857-61 [PMID:9756931]