PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT4G34590.1

Common Name

ATB2, AtbZIP11, BZIP11, GBF6, T4L20.170

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

bZIP

Protein Properties

Length: 159aa MW: 18035.2 Da PI: 5.2486

Description

G-box binding factor 6

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT4G34590.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	bZIP_1	38.7	2.2e-12	27	68	5	46
CHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH CS bZIP_1 5 krerrkqkNReAArrsRqRKkaeieeLeekvkeLeaeNkaLk 46 ++ +r+ +NRe+ArrsR +K++ ++ L+ v L++eN++ AT4G34590.1 27 RKRKRMLSNRESARRSRMKKQKLLDDLTAQVNHLKKENTEIV 68 7889**********************************9765 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:1.20.5.170	3.5E-10	21	68	No hit	No description
SMART	SM00338	5.6E-18	23	87	IPR004827	Basic-leucine zipper domain
PROSITE profile	PS50217	10.105	25	88	IPR004827	Basic-leucine zipper domain
Pfam	PF00170	1.6E-9	26	67	IPR004827	Basic-leucine zipper domain
SuperFamily	SSF57959	2.67E-12	27	80	No hit	No description
CDD	cd14702	6.43E-17	28	78	No hit	No description
PROSITE pattern	PS00036	0	30	45	IPR004827	Basic-leucine zipper domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009744	Biological Process	response to sucrose
GO:0080149	Biological Process	sucrose induced translational repression
GO:0005634	Cellular Component	nucleus
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding
GO:0046982	Molecular Function	protein heterodimerization activity

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000263	anatomy	non-hair root epidermal cell
PO:0000293	anatomy	guard cell

Sequence ? help Back to Top
Protein Sequence Length: 159 aa Download sequence Send to blast
MESSSSGTTS STIQTSSGSE ESLMEQRKRK RMLSNRESAR RSRMKKQKLL DDLTAQVNHL 60 KKENTEIVTS VSITTQHYLT VEAENSVLRA QLDELNHRLQ SLNDIIEFLD SSNNNNNNNM 120 GMCSNPLVGL ECDDFFVNQM NMSYIMNQPL MASSDALMY

Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	26	47	RKRKRMLSNRESARRSRMKKQK

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.135	0.0	bud\| floral meristem\| flower\| root\| seed\| silique

Expression -- Microarray ? help Back to Top
Source	ID	E-value
GEO	42567393	0.0
Genevisible	253245_at	0.0
Expression Atlas	AT4G34590	-
AtGenExpress	AT4G34590	-
ATTED-II	AT4G34590	-

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Highly expressed in stems and flowers (PubMed:9620274). Expressed in root tips, cotyledons, leaf vasculature, embryos, apical parts of siliques and funiculi (PubMed:9721683). {ECO:0000269\|PubMed:9620274, ECO:0000269\|PubMed:9721683}.

Functional Description ? help Back to Top
Source	Description
TAIR	Encodes a basic domain leucine zipper (bZip) transcription factor bZIP11. Translation is repressed by sucrose. Directly regulates gene expression of ASN1 and ProDH2, which are enzyme-coding genes involved in amino acid metabolism.
UniProt	Transcription factor that binds to the DNA sequence 5'-ACTCAT-3' in target gene promoters. Promotes POX1/PRODH1 expression in response to hypoosmolarity stress (PubMed:15047879). Positively regulates the expression of ASN1 and POX2/PRODH2 genes, which are involved in amino acid metabolism (PubMed:18088315). Regulates several metabolic pathways such as myo-inositol, raffinose and trehalose. Regulates several trehalose metabolism genes, including TRE1, TPP5 and TPP6 (PubMed:21534971). Mediates recruitment of the histone acetylation machinery to activate auxin-induced transcription. Interacts with ADA2B adapter protein to promote ADA2B-mediated recruitment of SAGA-like histone acetyltransferase complexes to specific auxin-responsive genes (PubMed:24861440). {ECO:0000269\|PubMed:15047879, ECO:0000269\|PubMed:18088315, ECO:0000269\|PubMed:21534971, ECO:0000269\|PubMed:24861440}.

Function -- GeneRIF ? help Back to Top
bZIP11 target processes are identified using transiently increased nuclear bZIP11 levels and genome-wide expression analysis; the effect of bZIP11 on ASN1 and GBF6 are reported. [PMID: 18088315] The uORF2 element in the bZip11 mRNA encodes a sucrose control peptide that inhibits bZIP11 translation in response to high sucrose levels by stalling the ribosome on the mRNA. [PMID: 19403731] The bZIP11 induction leads to reduced contents of the prominent growth regulatory molecule trehalose 6-phosphate (T6P). The metabolic changes detected mimic in part those observed in carbon-starved plants. [PMID: 21534971] Arabidopsis bZIP11-related basic leucine zipper (bZIP) transcription factors interact via an amino-terminal activation domain with ADA2b adapter proteins to recruit the histone acetylation machinery to specific auxin-responsive genes. [PMID: 24861440] Based on ensuing molecular and biochemical analyses, we propose a mechanistic model, in which bZIP11-related TFs gain control over the root meristem by directly activating IAA3/SHY2 transcription. IAA3/SHY2 is a pivotal negative regulator of root growth, which has been demonstrated to efficiently repress transcription of major auxin transport facilitators of the PIN-FORMED (PIN) gene family, thereby restricting polar auxi [PMID: 28158182] Data show that bZIP11 uORF2 encodes a regulatory nascent peptide that functions to sense intracellular sucrose abundance. [PMID: 28369795]

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00470	DAP	27203113	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT4G34590.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By light (PubMed:9620274). Induced by hypoosmolarity (PubMed:15047879). Repressed by sucrose (at protein level) (PubMed:9721683, PubMed:15208401). {ECO:0000269\|PubMed:15047879, ECO:0000269\|PubMed:15208401, ECO:0000269\|PubMed:9620274, ECO:0000269\|PubMed:9721683}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source	Target Gene (A: Activate/R: Repress)
ATRM	AT3G30775(A), AT3G47340(A)

Interaction ? help Back to Top
Source	Intact With
BioGRID	AT4G34590, AT5G24800, AT5G28770, AT5G49450, AT1G13600
IntAct	Search O65683

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT4G34590

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AL023094	0.0	AL023094.2 Arabidopsis thaliana DNA chromosome 4, BAC clone T4L20 (ESSA project).
GenBank	AL161585	0.0	AL161585.2 Arabidopsis thaliana DNA chromosome 4, contig fragment No. 81.
GenBank	AY063979	0.0	AY063979.1 Arabidopsis thaliana putative bZIP transcription factor ATB2 (At4g34590) mRNA, complete cds.
GenBank	AY096396	0.0	AY096396.1 Arabidopsis thaliana putative bZIP transcription factor ATB2 (At4g34590) mRNA, complete cds.
GenBank	CP002687	0.0	CP002687.1 Arabidopsis thaliana chromosome 4 sequence.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_195185.1	1e-113	G-box binding factor 6
Swissprot	O65683	1e-114	BZP11_ARATH; bZIP transcription factor 11
TrEMBL	A0A178UVA0	1e-112	A0A178UVA0_ARATH; Uncharacterized protein
STRING	AT4G34590.1	1e-112	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM501	28	154
Representative plant	OGRP551	16	78

Link Out ? help Back to Top
Phytozome	AT4G34590.1
Entrez Gene	829611
iHOP	AT4G34590
wikigenes	AT4G34590

Publications ? help Back to Top
Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Jakoby M, et al. bZIP transcription factors in Arabidopsis. Trends Plant Sci., 2002. 7(3): p. 106-11 [PMID:11906833] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Satoh R,Fujita Y,Nakashima K,Shinozaki K,Yamaguchi-Shinozaki K A novel subgroup of bZIP proteins functions as transcriptional activators in hypoosmolarity-responsive expression of the ProDH gene in Arabidopsis. Plant Cell Physiol., 2004. 45(3): p. 309-17 [PMID:15047879] Wiese A,Elzinga N,Wobbes B,Smeekens S A conserved upstream open reading frame mediates sucrose-induced repression of translation. Plant Cell, 2004. 16(7): p. 1717-29 [PMID:15208401] Kim TH,Kim BH,Yahalom A,Chamovitz DA,von Arnim AG Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h. Plant Cell, 2004. 16(12): p. 3341-56 [PMID:15548739] Wiese A,Elzinga N,Wobbes B,Smeekens S Sucrose-induced translational repression of plant bZIP-type transcription factors. Biochem. Soc. Trans., 2005. 33(Pt 1): p. 272-5 [PMID:15667324] Ehlert A, et al. Two-hybrid protein-protein interaction analysis in Arabidopsis protoplasts: establishment of a heterodimerization map of group C and group S bZIP transcription factors. Plant J., 2006. 46(5): p. 890-900 [PMID:16709202] Weltmeier F, et al. Combinatorial control of Arabidopsis proline dehydrogenase transcription by specific heterodimerisation of bZIP transcription factors. EMBO J., 2006. 25(13): p. 3133-43 [PMID:16810321] Hanson J,Hanssen M,Wiese A,Hendriks MM,Smeekens S The sucrose regulated transcription factor bZIP11 affects amino acid metabolism by regulating the expression of ASPARAGINE SYNTHETASE1 and PROLINE DEHYDROGENASE2. Plant J., 2008. 53(6): p. 935-49 [PMID:18088315] Adamiec M,Drath M,Jackowski G Redox state of plastoquinone pool regulates expression of Arabidopsis thaliana genes in response to elevated irradiance. Acta Biochim. Pol., 2008. 55(1): p. 161-73 [PMID:18231654] Hummel M,Rahmani F,Smeekens S,Hanson J Sucrose-mediated translational control. Ann. Bot., 2009. 104(1): p. 1-7 [PMID:19376782] Rahmani F, et al. Sucrose control of translation mediated by an upstream open reading frame-encoded peptide. Plant Physiol., 2009. 150(3): p. 1356-67 [PMID:19403731] Roy B, et al. The h subunit of eIF3 promotes reinitiation competence during translation of mRNAs harboring upstream open reading frames. RNA, 2010. 16(4): p. 748-61 [PMID:20179149] Zhou F,Roy B,von Arnim AG Translation reinitiation and development are compromised in similar ways by mutations in translation initiation factor eIF3h and the ribosomal protein RPL24. BMC Plant Biol., 2010. 10: p. 193 [PMID:20799971] Brady SM, et al. A stele-enriched gene regulatory network in the Arabidopsis root. Mol. Syst. Biol., 2011. 7: p. 459 [PMID:21245844] Ma J, et al. The sucrose-regulated Arabidopsis transcription factor bZIP11 reprograms metabolism and regulates trehalose metabolism. New Phytol., 2011. 191(3): p. 733-45 [PMID:21534971] Delatte TL, et al. Growth arrest by trehalose-6-phosphate: an astonishing case of primary metabolite control over growth by way of the SnRK1 signaling pathway. Plant Physiol., 2011. 157(1): p. 160-74 [PMID:21753116] Adamiec M,Luciński R,Jackowski G The irradiance dependent transcriptional regulation of AtCLPB3 expression. Plant Sci., 2011. 181(4): p. 449-56 [PMID:21889051] Bruex A, et al. A gene regulatory network for root epidermis cell differentiation in Arabidopsis. PLoS Genet., 2012. 8(1): p. e1002446 [PMID:22253603] Thalor SK, et al. Deregulation of sucrose-controlled translation of a bZIP-type transcription factor results in sucrose accumulation in leaves. PLoS ONE, 2012. 7(3): p. e33111 [PMID:22457737] Iglesias-Fern Arabidopsis thaliana bZIP44: a transcription factor affecting seed germination and expression of the mannanase-encoding gene AtMAN7. Plant J., 2013. 74(5): p. 767-80 [PMID:23461773] Nunes C, et al. The trehalose 6-phosphate/SnRK1 signaling pathway primes growth recovery following relief of sink limitation. Plant Physiol., 2013. 162(3): p. 1720-32 [PMID:23735508] Weiste C,Dr The Arabidopsis transcription factor bZIP11 activates auxin-mediated transcription by recruiting the histone acetylation machinery. Nat Commun, 2014. 5: p. 3883 [PMID:24861440] Jin J, et al. An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors. Mol. Biol. Evol., 2015. 32(7): p. 1767-73 [PMID:25750178] Mair A, et al. SnRK1-triggered switch of bZIP63 dimerization mediates the low-energy response in plants. Elife, 2016. [PMID:26263501] Sagor GH, et al. A novel strategy to produce sweeter tomato fruits with high sugar contents by fruit-specific expression of a single bZIP transcription factor gene. Plant Biotechnol. J., 2016. 14(4): p. 1116-26 [PMID:26402509] Walper E,Weiste C,Mueller MJ,Hamberg M,Dröge-Laser W Screen Identifying Arabidopsis Transcription Factors Involved in the Response to 9-Lipoxygenase-Derived Oxylipins. PLoS ONE, 2016. 11(4): p. e0153216 [PMID:27073862] Wang XY, et al. Metabolomic analysis reveals the relationship between AZI1 and sugar signaling in systemic acquired resistance of Arabidopsis. Plant Physiol. Biochem., 2016. 107: p. 273-287 [PMID:27337039] Weiste C, et al. The Arabidopsis bZIP11 transcription factor links low-energy signalling to auxin-mediated control of primary root growth. PLoS Genet., 2017. 13(2): p. e1006607 [PMID:28158182] Yamashita Y, et al. Sucrose sensing through nascent peptide-meditated ribosome stalling at the stop codon of Arabidopsis bZIP11 uORF2. FEBS Lett., 2017. 591(9): p. 1266-1277 [PMID:28369795] Ezer D, et al. The G-Box Transcriptional Regulatory Code in Arabidopsis. Plant Physiol., 2017. 175(2): p. 628-640 [PMID:28864470] Lee DH,Park SJ,Ahn CS,Pai HS MRF Family Genes Are Involved in Translation Control, Especially under Energy-Deficient Conditions, and Their Expression and Functions Are Modulated by the TOR Signaling Pathway. Plant Cell, 2017. 29(11): p. 2895-2920 [PMID:29084871] Pedrotti L, et al. Snf1-RELATED KINASE1-Controlled C/S1-bZIP Signaling Activates Alternative Mitochondrial Metabolic Pathways to Ensure Plant Survival in Extended Darkness. Plant Cell, 2018. 30(2): p. 495-509 [PMID:29348240] van Drunen CM, et al. Analysis of the chromatin domain organisation around the plastocyanin gene reveals an MAR-specific sequence element in Arabidopsis thaliana. Nucleic Acids Res., 1997. 25(19): p. 3904-11 [PMID:9380515] Rook F,Weisbeek P,Smeekens S The light-regulated Arabidopsis bZIP transcription factor gene ATB2 encodes a protein with an unusually long leucine zipper domain. Plant Mol. Biol., 1998. 37(1): p. 171-8 [PMID:9620274] Rook F, et al. Sucrose-specific signalling represses translation of the Arabidopsis ATB2 bZIP transcription factor gene. Plant J., 1998. 15(2): p. 253-63 [PMID:9721683]