PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT4G24020.1

Common Name

NLP7, T19F6.16

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

Nin-like

Protein Properties

Length: 959aa MW: 105742 Da PI: 5.6877

Description

NIN like protein 7

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT4G24020.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	RWP-RK	91.9	4.9e-29	589	639	2	52
RWP-RK 2 ekeisledlskyFslpikdAAkeLgvclTvLKriCRqyGIkRWPhRkiksl 52 ek+isl++l++yF +++kdAAk+Lgvc+T++KriCRq+GI+RWP+Rkik++ AT4G24020.1 589 EKTISLDVLQQYFTGSLKDAAKSLGVCPTTMKRICRQHGISRWPSRKIKKV 639 799**********************************************97 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SuperFamily	SSF55781	4.81E-5	214	283	IPR029016	GAF domain-like
PROSITE profile	PS51519	17.757	578	659	IPR003035	RWP-RK domain
Pfam	PF02042	7.3E-26	591	639	IPR003035	RWP-RK domain
SuperFamily	SSF54277	5.23E-23	860	948	No hit	No description
SMART	SM00666	8.9E-28	863	945	IPR000270	PB1 domain
Gene3D	G3DSA:3.10.20.240	1.3E-26	863	945	No hit	No description
PROSITE profile	PS51745	27.429	863	945	IPR000270	PB1 domain
Pfam	PF00564	8.5E-20	863	944	IPR000270	PB1 domain
CDD	cd06407	6.30E-34	864	944	No hit	No description

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009414	Biological Process	response to water deprivation
GO:0010118	Biological Process	stomatal movement
GO:0010167	Biological Process	response to nitrate
GO:0042128	Biological Process	nitrate assimilation
GO:0005634	Cellular Component	nucleus
GO:0003677	Molecular Function	DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0005515	Molecular Function	protein binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000013	anatomy	cauline leaf
PO:0000037	anatomy	shoot apex
PO:0000039	anatomy	shoot axis vascular system
PO:0000046	anatomy	seminal root
PO:0000074	anatomy	parenchyma cell
PO:0000230	anatomy	inflorescence meristem
PO:0000256	anatomy	root hair cell
PO:0000293	anatomy	guard cell
PO:0002000	anatomy	stomatal complex
PO:0008019	anatomy	leaf lamina base
PO:0009005	anatomy	root
PO:0009006	anatomy	shoot system
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009025	anatomy	vascular leaf
PO:0009029	anatomy	stamen
PO:0009030	anatomy	carpel
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0009052	anatomy	flower pedicel
PO:0020030	anatomy	cotyledon
PO:0020038	anatomy	petiole
PO:0020100	anatomy	hypocotyl
PO:0020137	anatomy	leaf apex
PO:0025022	anatomy	collective leaf structure
PO:0025281	anatomy	pollen
PO:0001054	developmental stage	vascular leaf senescent stage
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0001081	developmental stage	mature plant embryo stage
PO:0001185	developmental stage	plant embryo globular stage
PO:0004507	developmental stage	plant embryo bilateral stage
PO:0007064	developmental stage	LP.12 twelve leaves visible stage
PO:0007095	developmental stage	LP.08 eight leaves visible stage
PO:0007098	developmental stage	LP.02 two leaves visible stage
PO:0007103	developmental stage	LP.10 ten leaves visible stage
PO:0007115	developmental stage	LP.04 four leaves visible stage
PO:0007123	developmental stage	LP.06 six leaves visible stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 959 aa Download sequence Send to blast
MCEPDDNSAR NGVTTQPSRS RELLMDVDDL DLDGSWPLDQ IPYLSSSNRM ISPIFVSSSS 60 EQPCSPLWAF SDGGGNGFHH ATSGGDDEKI SSVSGVPSFR LAEYPLFLPY SSPSAAENTT 120 EKHNSFQFPS PLMSLVPPEN TDNYCVIKER MTQALRYFKE STEQHVLAQV WAPVRKNGRD 180 LLTTLGQPFV LNPNGNGLNQ YRMISLTYMF SVDSESDVEL GLPGRVFRQK LPEWTPNVQY 240 YSSKEFSRLD HALHYNVRGT LALPVFNPSG QSCIGVVELI MTSEKIHYAP EVDKVCKALE 300 AVNLKSSEIL DHQTTQICNE SRQNALAEIL EVLTVVCETH NLPLAQTWVP CQHGSVLANG 360 GGLKKNCTSF DGSCMGQICM STTDMACYVV DAHVWGFRDA CLEHHLQKGQ GVAGRAFLNG 420 GSCFCRDITK FCKTQYPLVH YALMFKLTTC FAISLQSSYT GDDSYILEFF LPSSITDDQE 480 QDLLLGSILV TMKEHFQSLR VASGVDFGED DDKLSFEIIQ ALPDKKVHSK IESIRVPFSG 540 FKSNATETML IPQPVVQSSD PVNEKINVAT VNGVVKEKKK TEKKRGKTEK TISLDVLQQY 600 FTGSLKDAAK SLGVCPTTMK RICRQHGISR WPSRKIKKVN RSITKLKRVI ESVQGTDGGL 660 DLTSMAVSSI PWTHGQTSAQ PLNSPNGSKP PELPNTNNSP NHWSSDHSPN EPNGSPELPP 720 SNGHKRSRTV DESAGTPTSH GSCDGNQLDE PKVPNQDPLF TVGGSPGLLF PPYSRDHDVS 780 AASFAMPNRL LGSIDHFRGM LIEDAGSSKD LRNLCPTAAF DDKFQDTNWM NNDNNSNNNL 840 YAPPKEEAIA NVACEPSGSE MRTVTIKASY KDDIIRFRIS SGSGIMELKD EVAKRLKVDA 900 GTFDIKYLDD DNEWVLIACD ADLQECLEIP RSSRTKIVRL LVHDVTTNLG SSCESTGEL

Sequence ? help Back to Top

Protein Sequence Length: 959 aa Download sequence Send to blast

MCEPDDNSAR NGVTTQPSRS RELLMDVDDL DLDGSWPLDQ IPYLSSSNRM ISPIFVSSSS  60
EQPCSPLWAF SDGGGNGFHH ATSGGDDEKI SSVSGVPSFR LAEYPLFLPY SSPSAAENTT  120
EKHNSFQFPS PLMSLVPPEN TDNYCVIKER MTQALRYFKE STEQHVLAQV WAPVRKNGRD  180
LLTTLGQPFV LNPNGNGLNQ YRMISLTYMF SVDSESDVEL GLPGRVFRQK LPEWTPNVQY  240
YSSKEFSRLD HALHYNVRGT LALPVFNPSG QSCIGVVELI MTSEKIHYAP EVDKVCKALE  300
AVNLKSSEIL DHQTTQICNE SRQNALAEIL EVLTVVCETH NLPLAQTWVP CQHGSVLANG  360
GGLKKNCTSF DGSCMGQICM STTDMACYVV DAHVWGFRDA CLEHHLQKGQ GVAGRAFLNG  420
GSCFCRDITK FCKTQYPLVH YALMFKLTTC FAISLQSSYT GDDSYILEFF LPSSITDDQE  480
QDLLLGSILV TMKEHFQSLR VASGVDFGED DDKLSFEIIQ ALPDKKVHSK IESIRVPFSG  540
FKSNATETML IPQPVVQSSD PVNEKINVAT VNGVVKEKKK TEKKRGKTEK TISLDVLQQY  600
FTGSLKDAAK SLGVCPTTMK RICRQHGISR WPSRKIKKVN RSITKLKRVI ESVQGTDGGL  660
DLTSMAVSSI PWTHGQTSAQ PLNSPNGSKP PELPNTNNSP NHWSSDHSPN EPNGSPELPP  720
SNGHKRSRTV DESAGTPTSH GSCDGNQLDE PKVPNQDPLF TVGGSPGLLF PPYSRDHDVS  780
AASFAMPNRL LGSIDHFRGM LIEDAGSSKD LRNLCPTAAF DDKFQDTNWM NNDNNSNNNL  840
YAPPKEEAIA NVACEPSGSE MRTVTIKASY KDDIIRFRIS SGSGIMELKD EVAKRLKVDA  900
GTFDIKYLDD DNEWVLIACD ADLQECLEIP RSSRTKIVRL LVHDVTTNLG SSCESTGEL

Expression -- Microarray ? help Back to Top
Source	ID	E-value
GEO	186512980	0.0
Genevisible	254195_at	0.0
Expression Atlas	AT4G24020	-
AtGenExpress	AT4G24020	-
ATTED-II	AT4G24020	-

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Expressed in roots, stems, leaves, flowers and siliques. Detected in root hairs, emerging secondary roots, vascular tissues, leaf parenchyma cells and stomata. {ECO:0000269\|PubMed:18826430}.

Functional Description ? help Back to Top
Source	Description
TAIR	Encodes NIN Like Protein 7 (NLP7). Modulates nitrate sensing and metabolism. Mutants of NLP7 show features of nitrogen-starved plants and are tolerant to drought stress. Localized in the nucleus and functions as a putative transcription factor.
UniProt	Transcription factor involved in regulation of nitrate assimilation and in transduction of the nitrate signal. {ECO:0000269\|PubMed:18826430}.

Function -- GeneRIF ? help Back to Top
NLP7 is an important element of the nitrate signal transduction pathway and is proposed as a new regulatory protein specific for nitrogen assimilation in non-nodulating plants. [NLP7] [PMID: 18826430] NLP7 expression maintains pectin and cellulose levels in the root and represses the expression of CEL5, XTH5, PL, SMB, and BRN1/2, thereby preventing single cell border-like cell release. [PMID: 27221617] Results demonstrate that NLP7 significantly improves plant growth under both nitrogen-poor and -rich conditions by coordinately enhancing nitrogen and carbon assimilation and sheds light on crop improvement. [PMID: 27293103] TCP20 and NLP6/NLP7 transcription factors control plant responses to nitrate availability. [PMID: 28202720] nitrate-coupled CPK signalling phosphorylates conserved NIN-LIKE PROTEIN (NLP) transcription factors to specify the reprogramming of gene sets for downstream transcription factors, transporters, nitrogen assimilation, carbon/nitrogen metabolism, redox, signalling, hormones and proliferation [PMID: 28489820] NLP7 control of root cap cell release is largely independent of gravity sensing and root cap cell identity. [PMID: 29215953]

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00449	DAP	27203113	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT4G24020.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Not regulated by the N source or by nitrate. {ECO:0000269\|PubMed:18826430}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source	Target Gene (A: Activate/R: Repress)
ATRM	AT1G08100(A)

Phenotype -- Disruption Phenotype ? help Back to Top
Source	Description
UniProt	DISRUPTION PHENOTYPE: N-starvation phenotype. Smaller rosette, but no effects on roots. Delayed growth and flowering when grown in greenhouse. Increased drought resistance. {ECO:0000269\|PubMed:18826430}.

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT4G24020

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AK228452	0.0	AK228452.1 Arabidopsis thaliana mRNA for hypothetical protein, complete cds, clone: RAFL15-03-P13.
GenBank	BT005776	0.0	BT005776.1 Arabidopsis thaliana clone RAFL15-03-P13 (R20174) unknown protein (At4g24020) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_194133.1	0.0	NIN like protein 7
Swissprot	Q84TH9	0.0	NLP7_ARATH; Protein NLP7
TrEMBL	A0A178V1Z0	0.0	A0A178V1Z0_ARATH; NLP7
STRING	AT4G24020.1	0.0	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM2507	26	66
Representative plant	OGRP299	16	97

Link Out ? help Back to Top
Phytozome	AT4G24020.1
Entrez Gene	828502
iHOP	AT4G24020
wikigenes	AT4G24020

Publications ? help Back to Top
Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Menges M,Hennig L,Gruissem W,Murray JA Cell cycle-regulated gene expression in Arabidopsis. J. Biol. Chem., 2002. 277(44): p. 41987-2002 [PMID:12169696] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Scheible WR, et al. Genome-wide reprogramming of primary and secondary metabolism, protein synthesis, cellular growth processes, and the regulatory infrastructure of Arabidopsis in response to nitrogen. Plant Physiol., 2004. 136(1): p. 2483-99 [PMID:15375205] Schauser L,Wieloch W,Stougaard J Evolution of NIN-like proteins in Arabidopsis, rice, and Lotus japonicus. J. Mol. Evol., 2005. 60(2): p. 229-37 [PMID:15785851] Castaings L, et al. The nodule inception-like protein 7 modulates nitrate sensing and metabolism in Arabidopsis. Plant J., 2009. 57(3): p. 426-35 [PMID:18826430] Wang R,Xing X,Wang Y,Tran A,Crawford NM A genetic screen for nitrate regulatory mutants captures the nitrate transporter gene NRT1.1. Plant Physiol., 2009. 151(1): p. 472-8 [PMID:19633234] Hachiya T, et al. Evidence for a nitrate-independent function of the nitrate sensor NRT1.1 in Arabidopsis thaliana. J. Plant Res., 2011. 124(3): p. 425-30 [PMID:21052766] Konishi M,Yanagisawa S Arabidopsis NIN-like transcription factors have a central role in nitrate signalling. Nat Commun, 2013. 4: p. 1617 [PMID:23511481] Jin J, et al. An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors. Mol. Biol. Evol., 2015. 32(7): p. 1767-73 [PMID:25750178] Xu N, et al. The Arabidopsis NRG2 Protein Mediates Nitrate Signaling and Interacts with and Regulates Key Nitrate Regulators. Plant Cell, 2016. 28(2): p. 485-504 [PMID:26744214] Karve R,Suárez-Román F,Iyer-Pascuzzi AS The Transcription Factor NIN-LIKE PROTEIN7 Controls Border-Like Cell Release. Plant Physiol., 2016. 171(3): p. 2101-11 [PMID:27221617] Yu LH, et al. Overexpression of Arabidopsis NLP7 improves plant growth under both nitrogen-limiting and -sufficient conditions by enhancing nitrogen and carbon assimilation. Sci Rep, 2016. 6: p. 27795 [PMID:27293103] Menz J,Li Z,Schulze WX,Ludewig U Early nitrogen-deprivation responses in Arabidopsis roots reveal distinct differences on transcriptome and (phospho-) proteome levels between nitrate and ammonium nutrition. Plant J., 2016. 88(5): p. 717-734 [PMID:27419465] Yan D, et al. NIN-like protein 8 is a master regulator of nitrate-promoted seed germination in Arabidopsis. Nat Commun, 2016. 7: p. 13179 [PMID:27731416] Guan P, et al. Interacting TCP and NLP transcription factors control plant responses to nitrate availability. Proc. Natl. Acad. Sci. U.S.A., 2017. 114(9): p. 2419-2424 [PMID:28202720] Liu KH, et al. Discovery of nitrate-CPK-NLP signalling in central nutrient-growth networks. Nature, 2017. 545(7654): p. 311-316 [PMID:28489820] Li Z, et al. The Arabidopsis CPSF30-L gene plays an essential role in nitrate signaling and regulates the nitrate transceptor gene NRT1.1. New Phytol., 2017. 216(4): p. 1205-1222 [PMID:28850721] Cao H, et al. Overexpression of the Maize ZmNLP6 and ZmNLP8 Can Complement the Arabidopsis Nitrate Regulatory Mutant nlp7 by Restoring Nitrate Signaling and Assimilation. Front Plant Sci, 2017. 8: p. 1703 [PMID:29051766] Konishi N,Okubo T,Yamaya T,Hayakawa T,Minamisawa K Nitrate Supply-Dependent Shifts in Communities of Root-Associated Bacteria in Arabidopsis. Microbes Environ., 2017. 32(4): p. 314-323 [PMID:29187692] Karve RA,Iyer-Pascuzzi AS Further insights into the role of NIN-LIKE PROTEIN 7 (NLP7) in root cap cell release. Plant Signal Behav, 2018. 13(1): p. e1414122 [PMID:29215953] Zhao L, et al. The Arabidopsis NLP7 gene regulates nitrate signaling via NRT1.1-dependent pathway in the presence of ammonium. Sci Rep, 2018. 8(1): p. 1487 [PMID:29367694]