PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT4G00220.1
Common NameASL19, F6N15.4, JLO, LBD30
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family LBD
Protein Properties Length: 228aa    MW: 23968.1 Da    PI: 7.2496
Description LBD family protein
Gene Model
Gene Model ID Type Source Coding Sequence
AT4G00220.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
       DUF260   1 aCaaCkvlrrkCakdCvlapyfpaeq.pkkfanvhklFGasnvlkllkalpeeeredamsslvyeAearardPvyGavgvilklqqqleqlkaelall 97 
                  +C aCk+lrrkC+++C++apyf +eq +++fa+vhk+FGasnv+kll+++pe++r+da+ s+++eA+ar+rdP+yG+v++i +lqqq+ +l++el++l
                  7***********************9989********************************************************************99 PP

       DUF260  98 kee 100
  AT4G00220.1 115 QAH 117
                  986 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PROSITE profilePS5089123.68816118IPR004883Lateral organ boundaries, LOB
PfamPF031958.8E-4017115IPR004883Lateral organ boundaries, LOB
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0010089Biological Processxylem development
GO:0010199Biological Processorgan boundary specification between lateral organs and the meristem
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000019anatomygynoecium primordium
PO:0000229anatomyflower meristem
PO:0000230anatomyinflorescence meristem
PO:0000290anatomytracheary element
PO:0004704anatomysepal primordium
PO:0004705anatomystamen primordium
PO:0020003anatomyplant ovule
PO:0007611developmental stagepetal differentiation and expansion stage
Sequence ? help Back to Top
Protein Sequence    Length: 228 aa     Download sequence    Send to blast
3D Structure ? help Back to Top
PDB ID Evalue Query Start Query End Hit Start Hit End Description
5ly0_A1e-38101184111LOB family transfactor Ramosa2.1
5ly0_B1e-38101184111LOB family transfactor Ramosa2.1
Search in ModeBase
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.211820.0flower| silique
Expression -- Microarray ? help Back to Top
Source ID E-value
Expression AtlasAT4G00220-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Expressed in roots, stems, leaves and flowers (PubMed:12068116). Expressed in vascular tissues of hypocotyls, leaves, roots, developing floral organs and siliques (PubMed:19088331). {ECO:0000269|PubMed:12068116, ECO:0000269|PubMed:19088331}.
Functional Description ? help Back to Top
Source Description
TAIREncodes a protein containing a LOB domain that is expressed in embryos, flower primordium and lateral floral organ boundaries. Overexpression is correlated with activation of STM and KNAT1 and down regulation of PIN1 and reduced auxin transport levels. Ectopic expression in plants results in premature termination of the shoot apical meristem and small, lobed leaves.
UniProtInvolved in the positive regulation of tracheary element (TE) differentiation. Involved in a positive feedback loop that maintains or promotes NAC030/VND7 expression that regulates TE differentiation-related genes. {ECO:0000269|PubMed:19088331}.
Function -- GeneRIF ? help Back to Top
  1. Jagged Lateral Organs (JLO) acts from the boundary to orchestrate the drastic changes in gene expression that entail the initiation of plant lateral organs. [JLO]
    [PMID: 17557810]
  2. ASL19 were expressed in immature tracheary elements (TEs), and the expression was dependent on VASCULAR-RELATED NAC-DOMAIN PROTEIN6 (VND6) and VND7, which are transcription factors required for TE differentiation.
    [PMID: 19088331]
  3. JLO is a central regulator of auxin distribution and signaling throughout plant development.
    [PMID: 20852917]
  4. The JLO and AS2 proteins interact molecularly and form multimeric complexes with AS1 to suppress KNOX expression. AS2 together with JLO regulate auxin transport in seedling roots.
    [PMID: 22822207]
  5. Data indicate that the transcript levels of HAK5 and four transcriptin factor genes DDF2, JLO, bHLH121 and TFII_A were highly up-regulated in response to both -K and salt stress.
    [PMID: 23825216]
  6. JLO can regulate auxin perception and consequently auxin transport and auxin-responsive gene expression
    [PMID: 28472464]
  7. Genes controlling SCW formation were activated by the overexpression of LBD30, but not in the LBD30(K226R) mutant. LBD30 enhancement of SCW formation resulted from upregulation of SND1/NST1-mediated transcriptional networks. This study presents a mechanism by which sumoylation of LBD30, mediated by SIZ1, regulates SCW formation in A. thaliana.
    [PMID: 30657769]
Cis-element ? help Back to Top
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT1G23080(R), AT1G62360(A), AT1G70940(R), AT1G73590(R), AT2G01420(R), AT2G37630(R), AT4G08150(A), AT4G34160(A), AT5G67260(A)
Interaction ? help Back to Top
Source Intact With
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT4G00220
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAB4738520.0AB473852.1 Arabidopsis thaliana ASL19 mRNA for ASYMMETRIC LEAVES2-like 19 protein, complete cds.
GenBankAF4322320.0AF432232.1 Arabidopsis thaliana LOB DOMAIN 30 (LBD30) mRNA, complete cds.
GenBankBT0264070.0BT026407.1 Arabidopsis thaliana At4g00220 mRNA, complete cds.
GenBankDQ4467870.0DQ446787.1 Arabidopsis thaliana clone pENTR221-At4g00220 LOB domain protein 30/lateral organ boundaries domain protein 30 (At4g00220) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_191933.11e-165Lateral organ boundaries (LOB) domain family protein
SwissprotO813231e-166LBD30_ARATH; LOB domain-containing protein 30
STRINGAT4G00220.11e-164(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Representative plantOGRP40515100
Publications ? help Back to Top
  1. Iwakawa H, et al.
    The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana, required for formation of a symmetric flat leaf lamina, encodes a member of a novel family of proteins characterized by cysteine repeats and a leucine zipper.
    Plant Cell Physiol., 2002. 43(5): p. 467-78
  2. Shuai B,Reynaga-Peña CG,Springer PS
    The lateral organ boundaries gene defines a novel, plant-specific gene family.
    Plant Physiol., 2002. 129(2): p. 747-61
  3. Underwood BA,Vanderhaeghen R,Whitford R,Town CD,Hilson P
    Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations.
    Plant Biotechnol. J., 2006. 4(3): p. 317-24
  4. Borghi L,Bureau M,Simon R
    Arabidopsis JAGGED LATERAL ORGANS is expressed in boundaries and coordinates KNOX and PIN activity.
    Plant Cell, 2007. 19(6): p. 1795-808
  5. Soyano T,Thitamadee S,Machida Y,Chua NH
    ASYMMETRIC LEAVES2-LIKE19/LATERAL ORGAN BOUNDARIES DOMAIN30 and ASL20/LBD18 regulate tracheary element differentiation in Arabidopsis.
    Plant Cell, 2008. 20(12): p. 3359-73
  6. Matsumura Y,Iwakawa H,Machida Y,Machida C
    Characterization of genes in the ASYMMETRIC LEAVES2/LATERAL ORGAN BOUNDARIES (AS2/LOB) family in Arabidopsis thaliana, and functional and molecular comparisons between AS2 and other family members.
    Plant J., 2009. 58(3): p. 525-37
  7. Bureau M,Simon R
    JLO regulates embryo patterning and organ initiation by controlling auxin transport.
    Plant Signal Behav, 2008. 3(2): p. 145-7
  8. Bureau M,Rast MI,Illmer J,Simon R
    JAGGED LATERAL ORGAN (JLO) controls auxin dependent patterning during development of the Arabidopsis embryo and root.
    Plant Mol. Biol., 2010. 74(4-5): p. 479-91
  9. Hsieh TF, et al.
    Regulation of imprinted gene expression in Arabidopsis endosperm.
    Proc. Natl. Acad. Sci. U.S.A., 2011. 108(5): p. 1755-62
  10. Rast MI,Simon R
    Arabidopsis JAGGED LATERAL ORGANS acts with ASYMMETRIC LEAVES2 to coordinate KNOX and PIN expression in shoot and root meristems.
    Plant Cell, 2012. 24(7): p. 2917-33
  11. Meinke DW
    A survey of dominant mutations in Arabidopsis thaliana.
    Trends Plant Sci., 2013. 18(2): p. 84-91
  12. Hong JP, et al.
    Identification and characterization of transcription factors regulating Arabidopsis HAK5.
    Plant Cell Physiol., 2013. 54(9): p. 1478-90
  13. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
  14. Rast-Somssich MI, et al.
    The Arabidopsis JAGGED LATERAL ORGANS (JLO) gene sensitizes plants to auxin.
    J. Exp. Bot., 2017. 68(11): p. 2741-2755
  15. Liu C,Yu H,Li L
    SUMO modification of LBD30 by SIZ1 regulates secondary cell wall formation in Arabidopsis thaliana.
    PLoS Genet., 2019. 15(1): p. e1007928