PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT3G27010.1
Common NameATTCP20, AT-TCP20, MOJ10.8, PCF1, TCP20
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family TCP
Protein Properties Length: 314aa    MW: 33390.9 Da    PI: 7.2565
Description TCP family protein
Gene Model
Gene Model ID Type Source Coding Sequence
AT3G27010.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
          TCP   3 gkkdrhskihTkvggRdRRvRlsaecaarfFdLqdeLGfdkdsktieWLlqqakpaikeltgtssssaseceaesssssasns 85 
                  +k+++++++hTkv+gR+RR+R++a+caar+F+L++eLG+++d++ti+WLlqqa+p+i+++tg+++++as+  a+s   s++++
                  6999****************************************************************776.23333333322 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PfamPF036342.4E-3277258IPR005333Transcription factor, TCP
PROSITE profilePS5136928.01678132IPR017887Transcription factor TCP subgroup
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0008361Biological Processregulation of cell size
GO:0009653Biological Processanatomical structure morphogenesis
GO:1900056Biological Processnegative regulation of leaf senescence
GO:0005634Cellular Componentnucleus
GO:0000987Molecular Functioncore promoter proximal region sequence-specific DNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005515Molecular Functionprotein binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0009009anatomyplant embryo
PO:0009025anatomyvascular leaf
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
Sequence ? help Back to Top
Protein Sequence    Length: 314 aa     Download sequence    Send to blast
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
Expression -- Microarray ? help Back to Top
Source ID E-value
Expression AtlasAT3G27010-
Functional Description ? help Back to Top
Source Description
TAIRBelongs to a TCP protein transcription factor family. Members of this family contain a predicted basic-helix-loop-helix domain involved in DNA binding. Related to rice PCF1 and PCF2 genes. Binds to the GCCCR element of CYCB1;1. Involved in regulation of expression of cell cycle control and ribosomal protein genes.
UniProtTranscription factor that binds to the site II motif (3'-TGGGCC/T-5') in the promoter of PCNA-2 and to 3'-GCCCG/A-5' elements in the promoters of cyclin CYCB1-1 and ribosomal protein genes. {ECO:0000269|PubMed:12631321, ECO:0000269|PubMed:16123132}.
Function -- GeneRIF ? help Back to Top
  1. p33(TCP20), encoded by the Arabidopsis TCP20 gene, binds to the GCCCR element in the promoters of cyclin CYCB1;1 [TCP20]
    [PMID: 16123132]
  2. AtTCP20 is involved in cell division and growth coordination.
    [PMID: 19091878]
  3. This study provides evidence for a mechanism whereby the transcriptional regulators SAC51 and TCP20 antagonistically regulate the expression of four subgroup Ib bHLH genes.
    [PMID: 24549883]
  4. TCP20 plays a key role in the systemic signaling pathway that directs nitrate foraging by Arabidopsis roots
    [PMID: 25288754]
  5. Data show that binding of LWD1 and TEOSINTE BRANCHED 1-CYCLOIDEA-PCF20 (TCP20) and TCP22 to the TCP-binding site in the CIRCADIAN CLOCK ASSOCIATED1 (CCA1) promoter activates CCA1.
    [PMID: 27734958]
  6. TCP20 and NLP6/NLP7 transcription factors control plant responses to nitrate availability.
    [PMID: 28202720]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
Motif logo
Cis-element ? help Back to Top
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
Interaction ? help Back to Top
Source Intact With
BioGRIDAT3G47620, AT4G18170, AT4G18390, AT4G27330, AT5G08070, AT5G08330, AT5G51910, AT5G60970, AT1G30210, AT1G35560, AT1G52890, AT1G53230, AT1G58100, AT1G69690
IntActSearch Q9LSD5
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT3G27010
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAB0266490.0AB026649.1 Arabidopsis thaliana genomic DNA, chromosome 3, P1 clone: MOJ10.
GenBankAJ2917490.0AJ291749.1 Arabidopsis thaliana mRNA for putative transcription factor (PCF1 gene).
GenBankAK1181780.0AK118178.1 Arabidopsis thaliana At3g27010 mRNA for unknown protein, complete cds, clone: RAFL19-49-P20.
GenBankBT0060430.0BT006043.1 Arabidopsis thaliana clone U50934 unknown protein (At3g27010) mRNA, complete cds.
GenBankCP0026860.0CP002686.1 Arabidopsis thaliana chromosome 3, complete sequence.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_001327814.10.0TEOSINTE BRANCHED 1, cycloidea, PCF (TCP)-domain family protein 20
RefseqNP_189337.10.0TEOSINTE BRANCHED 1, cycloidea, PCF (TCP)-domain family protein 20
SwissprotQ9LSD50.0TCP20_ARATH; Transcription factor TCP20
STRINGAT3G27010.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Representative plantOGRP9221459
Publications ? help Back to Top
  1. Cubas P,Lauter N,Doebley J,Coen E
    The TCP domain: a motif found in proteins regulating plant growth and development.
    Plant J., 1999. 18(2): p. 215-22
  2. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
  3. Seki M, et al.
    Functional annotation of a full-length Arabidopsis cDNA collection.
    Science, 2002. 296(5565): p. 141-5
  4. Kosugi S,Ohashi Y
    DNA binding and dimerization specificity and potential targets for the TCP protein family.
    Plant J., 2002. 30(3): p. 337-48
  5. Trémousaygue D, et al.
    Internal telomeric repeats and 'TCP domain' protein-binding sites co-operate to regulate gene expression in Arabidopsis thaliana cycling cells.
    Plant J., 2003. 33(6): p. 957-66
  6. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
  7. Li C,Potuschak T,Colón-Carmona A,Gutiérrez RA,Doerner P
    Arabidopsis TCP20 links regulation of growth and cell division control pathways.
    Proc. Natl. Acad. Sci. U.S.A., 2005. 102(36): p. 12978-83
  8. Vergnolle C, et al.
    The cold-induced early activation of phospholipase C and D pathways determines the response of two distinct clusters of genes in Arabidopsis cell suspensions.
    Plant Physiol., 2005. 139(3): p. 1217-33
  9. Andersson-Gunner
    Biosynthesis of cellulose-enriched tension wood in Populus: global analysis of transcripts and metabolites identifies biochemical and developmental regulators in secondary wall biosynthesis.
    Plant J., 2006. 45(2): p. 144-65
  10. Aguilar-Martínez JA,Poza-Carrión C,Cubas P
    Arabidopsis BRANCHED1 acts as an integrator of branching signals within axillary buds.
    Plant Cell, 2007. 19(2): p. 458-72
  11. Ascencio-Ib
    Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection.
    Plant Physiol., 2008. 148(1): p. 436-54
  12. Hervé C, et al.
    In vivo interference with AtTCP20 function induces severe plant growth alterations and deregulates the expression of many genes important for development.
    Plant Physiol., 2009. 149(3): p. 1462-77
  13. Viola IL,Uberti Manassero NG,Ripoll R,Gonzalez DH
    The Arabidopsis class I TCP transcription factor AtTCP11 is a developmental regulator with distinct DNA-binding properties due to the presence of a threonine residue at position 15 of the TCP domain.
    Biochem. J., 2011. 435(1): p. 143-55
  14. Viola IL,Reinheimer R,Ripoll R,Manassero NG,Gonzalez DH
    Determinants of the DNA binding specificity of class I and class II TCP transcription factors.
    J. Biol. Chem., 2012. 287(1): p. 347-56
  15. Danisman S, et al.
    Arabidopsis class I and class II TCP transcription factors regulate jasmonic acid metabolism and leaf development antagonistically.
    Plant Physiol., 2012. 159(4): p. 1511-23
  16. Efroni I, et al.
    Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses.
    Dev. Cell, 2013. 24(4): p. 438-45
  17. Danisman S, et al.
    Analysis of functional redundancies within the Arabidopsis TCP transcription factor family.
    J. Exp. Bot., 2013. 64(18): p. 5673-85
  18. Andriankaja ME, et al.
    Transcriptional coordination between leaf cell differentiation and chloroplast development established by TCP20 and the subgroup Ib bHLH transcription factors.
    Plant Mol. Biol., 2014. 85(3): p. 233-45
  19. Kim SH, et al.
    The Arabidopsis immune adaptor SRFR1 interacts with TCP transcription factors that redundantly contribute to effector-triggered immunity.
    Plant J., 2014. 78(6): p. 978-89
  20. Davi
    Class I TCP-DELLA interactions in inflorescence shoot apex determine plant height.
    Curr. Biol., 2014. 24(16): p. 1923-8
  21. We
    Convergent targeting of a common host protein-network by pathogen effectors from three kingdoms of life.
    Cell Host Microbe, 2014. 16(3): p. 364-75
  22. Guan P, et al.
    Nitrate foraging by Arabidopsis roots is mediated by the transcription factor TCP20 through the systemic signaling pathway.
    Proc. Natl. Acad. Sci. U.S.A., 2014. 111(42): p. 15267-72
  23. Chen GH,Sun JY,Liu M,Liu J,Yang WC
    SPOROCYTELESS is a novel embryophyte-specific transcription repressor that interacts with TPL and TCP proteins in Arabidopsis.
    J Genet Genomics, 2014. 41(12): p. 617-25
  24. Wang X, et al.
    TCP transcription factors are critical for the coordinated regulation of isochorismate synthase 1 expression in Arabidopsis thaliana.
    Plant J., 2015. 82(1): p. 151-62
  25. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
  26. Zhu P, et al.
    Arabidopsis small nucleolar RNA monitors the efficient pre-rRNA processing during ribosome biogenesis.
    Proc. Natl. Acad. Sci. U.S.A., 2016. 113(42): p. 11967-11972
  27. Wu JF, et al.
    LWD-TCP complex activates the morning gene CCA1 in Arabidopsis.
    Nat Commun, 2016. 7: p. 13181
  28. Guan P, et al.
    Interacting TCP and NLP transcription factors control plant responses to nitrate availability.
    Proc. Natl. Acad. Sci. U.S.A., 2017. 114(9): p. 2419-2424
  29. Guan P
    Dancing with Hormones: A Current Perspective of Nitrate Signaling and Regulation in Arabidopsis.
    Front Plant Sci, 2017. 8: p. 1697
  30. Zhang N, et al.
    MOS1 functions closely with TCP transcription factors to modulate immunity and cell cycle in Arabidopsis.
    Plant J., 2018. 93(1): p. 66-78
  31. Bresso EG,Chorostecki U,Rodriguez RE,Palatnik JF,Schommer C
    Spatial Control of Gene Expression by miR319-Regulated TCP Transcription Factors in Leaf Development.
    Plant Physiol., 2018. 176(2): p. 1694-1708
  32. Konishi N,Okubo T,Yamaya T,Hayakawa T,Minamisawa K
    Nitrate Supply-Dependent Shifts in Communities of Root-Associated Bacteria in Arabidopsis.
    Microbes Environ., 2017. 32(4): p. 314-323
  33. Kosugi S,Ohashi Y
    PCF1 and PCF2 specifically bind to cis elements in the rice proliferating cell nuclear antigen gene.
    Plant Cell, 1997. 9(9): p. 1607-19