PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT3G16770.1
Common NameATEBP, EBP, ERF072, ERF72, MGL6.1, MGL6.24, RAP2-3, RAP2.3
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family ERF
Protein Properties Length: 248aa    MW: 27758 Da    PI: 4.9441
Description ethylene-responsive element binding protein
Gene Model
Gene Model ID Type Source Coding Sequence
AT3G16770.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
          AP2   2 gykGVrwdkkrgrWvAeIrdpsengkrkrfslgkfgtaeeAakaaiaarkkleg 55 
                   y+G+r ++ +g+W+AeIrdp++     r++lg+f taeeAa a++ a+k+++g
                  69*******.**********954...4************************997 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SMARTSM003801.1E-3678141IPR001471AP2/ERF domain
PROSITE profilePS5103224.17178135IPR001471AP2/ERF domain
SuperFamilySSF541715.23E-2378136IPR016177DNA-binding domain
Gene3DG3DSA:3.30.730.102.0E-3278136IPR001471AP2/ERF domain
PfamPF008471.3E-1378127IPR001471AP2/ERF domain
PRINTSPR003671.6E-117990IPR001471AP2/ERF domain
CDDcd000183.09E-3279137No hitNo description
PRINTSPR003671.6E-11101117IPR001471AP2/ERF domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0008219Biological Processcell death
GO:0009723Biological Processresponse to ethylene
GO:0009735Biological Processresponse to cytokinin
GO:0009753Biological Processresponse to jasmonic acid
GO:0009873Biological Processethylene-activated signaling pathway
GO:0010286Biological Processheat acclimation
GO:0045893Biological Processpositive regulation of transcription, DNA-templated
GO:0051707Biological Processresponse to other organism
GO:0005634Cellular Componentnucleus
GO:0005737Cellular Componentcytoplasm
GO:0005886Cellular Componentplasma membrane
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005515Molecular Functionprotein binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009025anatomyvascular leaf
PO:0009052anatomyflower pedicel
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 248 aa     Download sequence    Send to blast
3D Structure ? help Back to Top
PDB ID Evalue Query Start Query End Hit Start Hit End Description
Search in ModeBase
Nucleic Localization Signal ? help Back to Top
No. Start End Sequence
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.247730.0flower| leaf| root| seed
Expression -- Microarray ? help Back to Top
Source ID E-value
Expression AtlasAT3G16770-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Mostly expressed in stems and leaves, and, to a lower extent, in roots, siliques and flowers. {ECO:0000269|PubMed:18836139, ECO:0000269|PubMed:9192694}.
Functional Description ? help Back to Top
Source Description
TAIREncodes a member of the ERF (ethylene response factor) subfamily B-2 of the plant specific ERF/AP2 transcription factor family (RAP2.3). The protein contains one AP2 domain. There are 5 members in this subfamily including RAP2.2 AND RAP2.12.It is localized to the nucleus and acts as a transcriptional activator through the GCC-box. It has been identified as a suppressor of Bax-induced cell death by functional screening in yeast and can also suppress Bax-induced cell death in tobacco plants. Overexpression of this gene in tobacco BY-2 cells confers resistance to H2O2 and heat stresses. Overexpression in Arabidopsis causes upregulation of PDF1.2 and GST6. It is part of the ethylene signaling pathway and is predicted to act downstream of EIN2 and CTR1, but not under EIN3.
UniProtProbably acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways (By similarity). {ECO:0000250, ECO:0000269|PubMed:9159183}.
Function -- GeneRIF ? help Back to Top
  1. Interaction of acyl-CoA-binding protein 4 with the ethylene-responsive element binding protein is induced by ethylene.
    [PMID: 18836139]
  2. AtEB domains bind to a target DNA motif with a universal CG step core recognition and different flanking bases preference
    [PMID: 19878300]
  3. RAP2.2, RAP2.3 and RAP2.12 play a role in modulating oxidative and osmotic stress tolerance during submergence acclimation.
    [PMID: 25847219]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
Motif logo
Cis-element ? help Back to Top
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: By 1-aminocyclopropane-1-carboxylic acid (ACC, ethylene precursor), methyl jasmonate (MeJA), and Botrytis cinerea. Also induced by cadmium. {ECO:0000269|PubMed:18836139, ECO:0000269|PubMed:9159183}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
Regulation -- ATRM (Manually Curated Upstream Regulators) ? help Back to Top
Source Upstream Regulator (A: Activate/R: Repress)
ATRM AT4G36920 (R)
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT2G47730(A), AT3G23240(A), AT4G36920(A), AT5G44420(A)
Regulation -- Hormone ? help Back to Top
Source Hormone
AHDcytokinin, ethylene
Interaction ? help Back to Top
Source Intact With
BioGRIDAT5G10030, AT1G14920
IntActSearch P42736
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT3G16770
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAF0030960.0AF003096.1 Arabidopsis thaliana AP2 domain containing protein RAP2.3 mRNA, complete cds.
GenBankAY0351000.0AY035100.1 Arabidopsis thaliana putative AP2 domain containing protein RAP2.3 (At3g16770) mRNA, complete cds.
GenBankAY0599170.0AY059917.1 Arabidopsis thaliana AP2 domain transcription factor RAP2.3 (MGL6.24) mRNA, complete cds.
GenBankAY0874880.0AY087488.1 Arabidopsis thaliana clone 35981 mRNA, complete sequence.
GenBankAY1425620.0AY142562.1 Arabidopsis thaliana putative AP2 domain containing protein RAP2.3 (At3g16770) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_188299.10.0ethylene-responsive element binding protein
SwissprotP427360.0RAP23_ARATH; Ethylene-responsive transcription factor RAP2-3
TrEMBLA0A178VEQ31e-156A0A178VEQ3_ARATH; RAP2.3
TrEMBLO231431e-156O23143_ARATH; Putative Ckc2
STRINGAT3G16770.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Representative plantOGRP6161718
Publications ? help Back to Top
  1. Kang HG,Fang Y,Singh KB
    A glucocorticoid-inducible transcription system causes severe growth defects in Arabidopsis and induces defense-related genes.
    Plant J., 1999. 20(1): p. 127-33
  2. Belles-Boix E,Babiychuk E,Van Montagu M,Inzé D,Kushnir S
    CEO1, a new protein from Arabidopsis thaliana, protects yeast against oxidative damage.
    FEBS Lett., 2000. 482(1-2): p. 19-24
  3. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
  4. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
  5. Li HY,Chye ML
    Arabidopsis Acyl-CoA-binding protein ACBP2 interacts with an ethylene-responsive element-binding protein, AtEBP, via its ankyrin repeats.
    Plant Mol. Biol., 2004. 54(2): p. 233-43
  6. Ogawa T, et al.
    Functional analysis of Arabidopsis ethylene-responsive element binding protein conferring resistance to Bax and abiotic stress-induced plant cell death.
    Plant Physiol., 2005. 138(3): p. 1436-45
  7. Brenner WG,Romanov GA,K
    Immediate-early and delayed cytokinin response genes of Arabidopsis thaliana identified by genome-wide expression profiling reveal novel cytokinin-sensitive processes and suggest cytokinin action through transcriptional cascades.
    Plant J., 2005. 44(2): p. 314-33
  8. Nakano T,Suzuki K,Fujimura T,Shinshi H
    Genome-wide analysis of the ERF gene family in Arabidopsis and rice.
    Plant Physiol., 2006. 140(2): p. 411-32
  9. Ogawa T,Uchimiya H,Kawai-Yamada M
    Mutual regulation of Arabidopsis thaliana ethylene-responsive element binding protein and a plant floral homeotic gene, APETALA2.
    Ann. Bot., 2007. 99(2): p. 239-44
  10. Bueso E, et al.
    The lithium tolerance of the Arabidopsis cat2 mutant reveals a cross-talk between oxidative stress and ethylene.
    Plant J., 2007. 52(6): p. 1052-65
  11. Ascencio-Ib
    Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection.
    Plant Physiol., 2008. 148(1): p. 436-54
  12. Li HY,Xiao S,Chye ML
    Ethylene- and pathogen-inducible Arabidopsis acyl-CoA-binding protein 4 interacts with an ethylene-responsive element binding protein.
    J. Exp. Bot., 2008. 59(14): p. 3997-4006
  13. Govrin EM,Rachmilevitch S,Tiwari BS,Solomon M,Levine A
    An Elicitor from Botrytis cinerea Induces the Hypersensitive Response in Arabidopsis thaliana and Other Plants and Promotes the Gray Mold Disease.
    Phytopathology, 2006. 96(3): p. 299-307
  14. Ceserani T,Trofka A,Gandotra N,Nelson T
    VH1/BRL2 receptor-like kinase interacts with vascular-specific adaptor proteins VIT and VIK to influence leaf venation.
    Plant J., 2009. 57(6): p. 1000-14
  15. Wang S, et al.
    Molecular dynamics simulations reveal the disparity in specific recognition of GCC-box by AtERFs transcription factors super family in Arabidopsis.
    J. Mol. Recognit., 2009 Nov-Dec. 22(6): p. 474-9
  16. Yang S, et al.
    Four divergent Arabidopsis ethylene-responsive element-binding factor domains bind to a target DNA motif with a universal CG step core recognition and different flanking bases preference.
    FEBS J., 2009. 276(23): p. 7177-86
  17. Ahn IP
    Disturbance of the Ca(2+)/calmodulin-dependent signalling pathway is responsible for the resistance of Arabidopsis dnd1 against Pectobacterium carotovorum infection.
    Mol. Plant Pathol., 2007. 8(6): p. 747-59
  18. Gao W,Li HY,Xiao S,Chye ML
    Protein interactors of acyl-CoA-binding protein ACBP2 mediate cadmium tolerance in Arabidopsis.
    Plant Signal Behav, 2010. 5(8): p. 1025-7
  19. S
    Determination of primary sequence specificity of Arabidopsis MAPKs MPK3 and MPK6 leads to identification of new substrates.
    Biochem. J., 2012. 446(2): p. 271-8
  20. Ding Y, et al.
    Four distinct types of dehydration stress memory genes in Arabidopsis thaliana.
    BMC Plant Biol., 2013. 13: p. 229
  21. Chen YC, et al.
    Root defense analysis against Fusarium oxysporum reveals new regulators to confer resistance.
    Sci Rep, 2014. 4: p. 5584
  22. Mar
    Large-scale identification of gibberellin-related transcription factors defines group VII ETHYLENE RESPONSE FACTORS as functional DELLA partners.
    Plant Physiol., 2014. 166(2): p. 1022-32
  23. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
  24. Papdi C, et al.
    The low oxygen, oxidative and osmotic stress responses synergistically act through the ethylene response factor VII genes RAP2.12, RAP2.2 and RAP2.3.
    Plant J., 2015. 82(5): p. 772-84
  25. Deslauriers SD,Alvarez AA,Lacey RF,Binder BM,Larsen PB
    Dominant gain-of-function mutations in transmembrane domain III of ERS1 and ETR1 suggest a novel role for this domain in regulating the magnitude of ethylene response in Arabidopsis.
    New Phytol., 2015. 208(2): p. 442-55
  26. Bui LT,Giuntoli B,Kosmacz M,Parlanti S,Licausi F
    Constitutively expressed ERF-VII transcription factors redundantly activate the core anaerobic response in Arabidopsis thaliana.
    Plant Sci., 2015. 236: p. 37-43
  27. Gasch P, et al.
    Redundant ERF-VII Transcription Factors Bind to an Evolutionarily Conserved cis-Motif to Regulate Hypoxia-Responsive Gene Expression in Arabidopsis.
    Plant Cell, 2016. 28(1): p. 160-80
  28. Liu W, et al.
    Ethylene response factor AtERF72 negatively regulates Arabidopsis thaliana response to iron deficiency.
    Biochem. Biophys. Res. Commun., 2017. 491(3): p. 862-868
  29. Yao Y,Chen X,Wu AM
    ERF-VII members exhibit synergistic and separate roles in Arabidopsis.
    Plant Signal Behav, 2017. 12(6): p. e1329073
  30. Büttner M,Singh KB
    Arabidopsis thaliana ethylene-responsive element binding protein (AtEBP), an ethylene-inducible, GCC box DNA-binding protein interacts with an ocs element binding protein.
    Proc. Natl. Acad. Sci. U.S.A., 1997. 94(11): p. 5961-6
  31. Okamuro JK,Caster B,Villarroel R,Van Montagu M,Jofuku KD
    The AP2 domain of APETALA2 defines a large new family of DNA binding proteins in Arabidopsis.
    Proc. Natl. Acad. Sci. U.S.A., 1997. 94(13): p. 7076-81