PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT3G14230.3

Common Name

ERF075, MLN21.1, RAP2-2, RAP2.2

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

ERF

Protein Properties

Length: 374aa MW: 41980.3 Da PI: 4.6519

Description

related to AP2 2

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT3G14230.3	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	AP2	62.7	8e-20	122	171	2	55
AP2 2 gykGVrwdkkrgrWvAeIrdpsengkrkrfslgkfgtaeeAakaaiaarkkleg 55 +y+G+r+++ +g+W+AeIrdp++ r +lg+f+taeeAa+a++aa+++++g AT3G14230.3 122 QYRGIRQRP-WGKWAAEIRDPRK---GSREWLGTFDTAEEAARAYDAAARRIRG 171 69*****.******954...39**********************98 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS51032	24.54	122	179	IPR001471	AP2/ERF domain
CDD	cd00018	2.43E-17	122	180	No hit	No description
Gene3D	G3DSA:3.30.730.10	2.4E-32	122	180	IPR001471	AP2/ERF domain
SuperFamily	SSF54171	2.42E-22	122	180	IPR016177	DNA-binding domain
SMART	SM00380	9.1E-40	122	185	IPR001471	AP2/ERF domain
PRINTS	PR00367	3.2E-11	123	134	IPR001471	AP2/ERF domain
Pfam	PF00847	9.7E-13	123	171	IPR001471	AP2/ERF domain
PRINTS	PR00367	3.2E-11	145	161	IPR001471	AP2/ERF domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0001666	Biological Process	response to hypoxia
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009873	Biological Process	ethylene-activated signaling pathway
GO:0010468	Biological Process	regulation of gene expression
GO:0005634	Cellular Component	nucleus
GO:0003677	Molecular Function	DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000013	anatomy	cauline leaf
PO:0000037	anatomy	shoot apex
PO:0000230	anatomy	inflorescence meristem
PO:0000293	anatomy	guard cell
PO:0008019	anatomy	leaf lamina base
PO:0009005	anatomy	root
PO:0009006	anatomy	shoot system
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009025	anatomy	vascular leaf
PO:0009029	anatomy	stamen
PO:0009030	anatomy	carpel
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0009052	anatomy	flower pedicel
PO:0020030	anatomy	cotyledon
PO:0020038	anatomy	petiole
PO:0020100	anatomy	hypocotyl
PO:0020137	anatomy	leaf apex
PO:0025022	anatomy	collective leaf structure
PO:0025281	anatomy	pollen
PO:0001054	developmental stage	vascular leaf senescent stage
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0001081	developmental stage	mature plant embryo stage
PO:0001185	developmental stage	plant embryo globular stage
PO:0004507	developmental stage	plant embryo bilateral stage
PO:0007064	developmental stage	LP.12 twelve leaves visible stage
PO:0007095	developmental stage	LP.08 eight leaves visible stage
PO:0007098	developmental stage	LP.02 two leaves visible stage
PO:0007103	developmental stage	LP.10 ten leaves visible stage
PO:0007115	developmental stage	LP.04 four leaves visible stage
PO:0007123	developmental stage	LP.06 six leaves visible stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 374 aa Download sequence Send to blast
MCGGAIISDF IPPPRSLRVT NEFIWPDLKN KVKASKKRSN KRSDFFDLDD DFEADFQGFK 60 DDSAFDCEDD DDVFVNVKPF VFTATTKPVA SAFVSTGSAY AKKTVESAEQ AEKSSKRKRK 120 NQYRGIRQRP WGKWAAEIRD PRKGSREWLG TFDTAEEAAR AYDAAARRIR GTKAKVNFPE 180 EKNPSVVSQK RPSAKTNNLQ KSVAKPNKSV TLVQQPTHLS QQYCNNSFDN SFGDMSFMEE 240 KPQMYNNQFG LTNSFDAGGN NGYQYFSSDQ GSNSFDCSEF GWSDHGPKTP EISSMLVNNN 300 EASFVEETNA AKKLKPNSDE SDDLMAYLDN ALWDTPLEVE AMLGADAGAV TQEEENPVEL 360 WSLDEINFML EGDF

Sequence ? help Back to Top

Protein Sequence Length: 374 aa Download sequence Send to blast

MCGGAIISDF IPPPRSLRVT NEFIWPDLKN KVKASKKRSN KRSDFFDLDD DFEADFQGFK  60
DDSAFDCEDD DDVFVNVKPF VFTATTKPVA SAFVSTGSAY AKKTVESAEQ AEKSSKRKRK  120
NQYRGIRQRP WGKWAAEIRD PRKGSREWLG TFDTAEEAAR AYDAAARRIR GTKAKVNFPE  180
EKNPSVVSQK RPSAKTNNLQ KSVAKPNKSV TLVQQPTHLS QQYCNNSFDN SFGDMSFMEE  240
KPQMYNNQFG LTNSFDAGGN NGYQYFSSDQ GSNSFDCSEF GWSDHGPKTP EISSMLVNNN  300
EASFVEETNA AKKLKPNSDE SDDLMAYLDN ALWDTPLEVE AMLGADAGAV TQEEENPVEL  360
WSLDEINFML EGDF

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
5wx9_A	5e-22	121	183	13	76	Ethylene-responsive transcription factor ERF096
Search in ModeBase

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.25146	0.0	bud\| flower\| leaf\| root\| seed\| silique\| vegetative tissue

Expression -- Microarray ? help Back to Top
Source	ID	E-value
GEO	30683054	0.0
Genevisible	258366_at	0.0
Expression Atlas	AT3G14230	-
AtGenExpress	AT3G14230	-
ATTED-II	AT3G14230	-

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Constitutive in flowers, leaves, stems, and roots. {ECO:0000269\|PubMed:20357136, ECO:0000269\|PubMed:9192694}.

Functional Description ? help Back to Top
Source	Description
TAIR	encodes a member of the ERF (ethylene response factor) subfamily B-2 of ERF/AP2 transcription factor family (RAP2.2). The protein contains one AP2 domain. There are 5 members in this subfamily including RAP2.2 AND RAP2.12.
UniProt	Transcription factor involved in carotenoid biosynthesis regulation. Binds to the 5'-ATCTA-3' element present in the promoter of phytoene synthase (PSY) and phytoene desaturase (PDS). Involved in ethylene response and resistance to necrotrophic pathogens. Acts as a downstream regulator in the ethylene signaling pathway. Partially redundant with RAP2-12. {ECO:0000269\|PubMed:17873090, ECO:0000269\|PubMed:20357136, ECO:0000269\|PubMed:22530619}.

Function -- GeneRIF ? help Back to Top
The action of SINAT2 may explain the recalcitrance of AtRAP2.2 protein levels to change by altering AtRAP2.2 transcription. [PMID: 17873090] Ethylene and RAP2.2, together with oxygen-dependent signal transduction, play an important role in the response to hypoxia. [PMID: 20357136] RAP2.2 functions as an important regulator in Botrytis resistance and ethylene responses. [PMID: 22530619] RAP2.2, RAP2.3 and RAP2.12 play a role in modulating oxidative and osmotic stress tolerance during submergence acclimation. [PMID: 25847219] An evolutionarily conserved 12-bp cis-regulatory motif that binds to and is sufficient for activation by RAP2.2 and RAP2.12 is identified. [PMID: 26668304] ERF74 and ERF75 play a redundant role in the up-regulation of RbohD transcription and enhance the ROS burst during the early stages of the stress response. [ERF75] [PMID: 28164334]

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT3G14230.3

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Up-regulated by darkness, ethylene and Botrytis cinerea. {ECO:0000269\|PubMed:20357136, ECO:0000269\|PubMed:22530619}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Interaction ? help Back to Top
Source	Intact With
BioGRID	AT1G10585, AT1G53170

Phenotype -- Disruption Phenotype ? help Back to Top
Source	Description
UniProt	DISRUPTION PHENOTYPE: Lethal when homozygous. {ECO:0000269\|PubMed:17873090}.

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT3G14230

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AY054539	0.0	AY054539.1 Arabidopsis thaliana transcription factor EREBP-like protein (At3g14230; MLN21.1) mRNA, complete cds.
GenBank	BT000374	0.0	BT000374.1 Arabidopsis thaliana transcription factor EREBP-like protein (At3g14230) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_850583.1	0.0	related to AP2 2
Swissprot	Q9LUM4	0.0	RAP22_ARATH; Ethylene-responsive transcription factor RAP2-2
TrEMBL	A0A178VN40	0.0	A0A178VN40_ARATH; RAP2.2
STRING	AT3G14230.1	0.0	(Arabidopsis thaliana)

Link Out ? help Back to Top
Phytozome	AT3G14230.3
Entrez Gene	820643
iHOP	AT3G14230
wikigenes	AT3G14230

Publications ? help Back to Top
Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Haas BJ, et al. Full-length messenger RNA sequences greatly improve genome annotation. Genome Biol., 2002. 3(6): p. RESEARCH0029 [PMID:12093376] Zik M,Irish VF Global identification of target genes regulated by APETALA3 and PISTILLATA floral homeotic gene action. Plant Cell, 2003. 15(1): p. 207-22 [PMID:12509532] Hirai MY, et al. Global expression profiling of sulfur-starved Arabidopsis by DNA macroarray reveals the role of O-acetyl-l-serine as a general regulator of gene expression in response to sulfur nutrition. Plant J., 2003. 33(4): p. 651-63 [PMID:12609039] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Hu W,Wang Y,Bowers C,Ma H Isolation, sequence analysis, and expression studies of florally expressed cDNAs in Arabidopsis. Plant Mol. Biol., 2003. 53(4): p. 545-63 [PMID:15010618] Nakayama N, et al. Gene trap lines define domains of gene regulation in Arabidopsis petals and stamens. Plant Cell, 2005. 17(9): p. 2486-506 [PMID:16055634] Nakano T,Suzuki K,Fujimura T,Shinshi H Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiol., 2006. 140(2): p. 411-32 [PMID:16407444] Mandaokar A, et al. Transcriptional regulators of stamen development in Arabidopsis identified by transcriptional profiling. Plant J., 2006. 46(6): p. 984-1008 [PMID:16805732] Brodersen P, et al. Arabidopsis MAP kinase 4 regulates salicylic acid- and jasmonic acid/ethylene-dependent responses via EDS1 and PAD4. Plant J., 2006. 47(4): p. 532-46 [PMID:16813576] Welsch R,Maass D,Voegel T,Dellapenna D,Beyer P Transcription factor RAP2.2 and its interacting partner SINAT2: stable elements in the carotenogenesis of Arabidopsis leaves. Plant Physiol., 2007. 145(3): p. 1073-85 [PMID:17873090] Ascencio-Ib Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection. Plant Physiol., 2008. 148(1): p. 436-54 [PMID:18650403] Hinz M, et al. Arabidopsis RAP2.2: an ethylene response transcription factor that is important for hypoxia survival. Plant Physiol., 2010. 153(2): p. 757-72 [PMID:20357136] Hanada K, et al. Functional compensation of primary and secondary metabolites by duplicate genes in Arabidopsis thaliana. Mol. Biol. Evol., 2011. 28(1): p. 377-82 [PMID:20736450] Ou B, et al. A high-throughput screening system for Arabidopsis transcription factors and its application to Med25-dependent transcriptional regulation. Mol Plant, 2011. 4(3): p. 546-55 [PMID:21343311] Zhao Y, et al. Arabidopsis RAP2.2 plays an important role in plant resistance to Botrytis cinerea and ethylene responses. New Phytol., 2012. 195(2): p. 450-60 [PMID:22530619] Efroni I, et al. Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses. Dev. Cell, 2013. 24(4): p. 438-45 [PMID:23449474] Lumba S, et al. A mesoscale abscisic acid hormone interactome reveals a dynamic signaling landscape in Arabidopsis. Dev. Cell, 2014. 29(3): p. 360-72 [PMID:24823379] Papdi C, et al. The low oxygen, oxidative and osmotic stress responses synergistically act through the ethylene response factor VII genes RAP2.12, RAP2.2 and RAP2.3. Plant J., 2015. 82(5): p. 772-84 [PMID:25847219] Bui LT,Giuntoli B,Kosmacz M,Parlanti S,Licausi F Constitutively expressed ERF-VII transcription factors redundantly activate the core anaerobic response in Arabidopsis thaliana. Plant Sci., 2015. 236: p. 37-43 [PMID:26025519] Gasch P, et al. Redundant ERF-VII Transcription Factors Bind to an Evolutionarily Conserved cis-Motif to Regulate Hypoxia-Responsive Gene Expression in Arabidopsis. Plant Cell, 2016. 28(1): p. 160-80 [PMID:26668304] Yao Y, et al. ETHYLENE RESPONSE FACTOR 74 (ERF74) plays an essential role in controlling a respiratory burst oxidase homolog D (RbohD)-dependent mechanism in response to different stresses in Arabidopsis. New Phytol., 2017. 213(4): p. 1667-1681 [PMID:28164334] Giuntoli B, et al. Age-dependent regulation of ERF-VII transcription factor activity in Arabidopsis thaliana. Plant Cell Environ., 2017. 40(10): p. 2333-2346 [PMID:28741696] Okamuro JK,Caster B,Villarroel R,Van Montagu M,Jofuku KD The AP2 domain of APETALA2 defines a large new family of DNA binding proteins in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A., 1997. 94(13): p. 7076-81 [PMID:9192694]