PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT3G14230.2
Common NameERF075, MLN21.1, RAP2-2, RAP2.2
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family ERF
Protein Properties Length: 375aa    MW: 42079.4 Da    PI: 4.6519
Description related to AP2 2
Gene Model
Gene Model ID Type Source Coding Sequence
AT3G14230.2genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
          AP2   2 gykGVrwdkkrgrWvAeIrdpsengkrkrfslgkfgtaeeAakaaiaarkkleg 55 
                  +y+G+r+++ +g+W+AeIrdp++     r +lg+f+taeeAa+a++aa+++++g
                  69*******.**********954...39************************98 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
CDDcd000182.46E-17123181No hitNo description
Gene3DG3DSA:3.30.730.102.4E-32123181IPR001471AP2/ERF domain
SuperFamilySSF541712.42E-22123181IPR016177DNA-binding domain
PROSITE profilePS5103224.54123180IPR001471AP2/ERF domain
SMARTSM003809.1E-40123186IPR001471AP2/ERF domain
PRINTSPR003673.2E-11124135IPR001471AP2/ERF domain
PfamPF008479.7E-13124172IPR001471AP2/ERF domain
PRINTSPR003673.2E-11146162IPR001471AP2/ERF domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0001666Biological Processresponse to hypoxia
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009873Biological Processethylene-activated signaling pathway
GO:0010468Biological Processregulation of gene expression
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009025anatomyvascular leaf
PO:0009052anatomyflower pedicel
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 375 aa     Download sequence    Send to blast
3D Structure ? help Back to Top
PDB ID Evalue Query Start Query End Hit Start Hit End Description
5wx9_A4e-221221841376Ethylene-responsive transcription factor ERF096
Search in ModeBase
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.251460.0bud| flower| leaf| root| seed| silique| vegetative tissue
Expression -- Microarray ? help Back to Top
Source ID E-value
Expression AtlasAT3G14230-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Constitutive in flowers, leaves, stems, and roots. {ECO:0000269|PubMed:20357136, ECO:0000269|PubMed:9192694}.
Functional Description ? help Back to Top
Source Description
TAIRencodes a member of the ERF (ethylene response factor) subfamily B-2 of ERF/AP2 transcription factor family (RAP2.2). The protein contains one AP2 domain. There are 5 members in this subfamily including RAP2.2 AND RAP2.12.
UniProtTranscription factor involved in carotenoid biosynthesis regulation. Binds to the 5'-ATCTA-3' element present in the promoter of phytoene synthase (PSY) and phytoene desaturase (PDS). Involved in ethylene response and resistance to necrotrophic pathogens. Acts as a downstream regulator in the ethylene signaling pathway. Partially redundant with RAP2-12. {ECO:0000269|PubMed:17873090, ECO:0000269|PubMed:20357136, ECO:0000269|PubMed:22530619}.
Function -- GeneRIF ? help Back to Top
  1. The action of SINAT2 may explain the recalcitrance of AtRAP2.2 protein levels to change by altering AtRAP2.2 transcription.
    [PMID: 17873090]
  2. Ethylene and RAP2.2, together with oxygen-dependent signal transduction, play an important role in the response to hypoxia.
    [PMID: 20357136]
  3. RAP2.2 functions as an important regulator in Botrytis resistance and ethylene responses.
    [PMID: 22530619]
  4. RAP2.2, RAP2.3 and RAP2.12 play a role in modulating oxidative and osmotic stress tolerance during submergence acclimation.
    [PMID: 25847219]
  5. An evolutionarily conserved 12-bp cis-regulatory motif that binds to and is sufficient for activation by RAP2.2 and RAP2.12 is identified.
    [PMID: 26668304]
  6. ERF74 and ERF75 play a redundant role in the up-regulation of RbohD transcription and enhance the ROS burst during the early stages of the stress response. [ERF75]
    [PMID: 28164334]
Cis-element ? help Back to Top
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Up-regulated by darkness, ethylene and Botrytis cinerea. {ECO:0000269|PubMed:20357136, ECO:0000269|PubMed:22530619}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
Interaction ? help Back to Top
Source Intact With
BioGRIDAT1G10585, AT1G53170
Phenotype -- Disruption Phenotype ? help Back to Top
Source Description
UniProtDISRUPTION PHENOTYPE: Lethal when homozygous. {ECO:0000269|PubMed:17873090}.
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT3G14230
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAY0545390.0AY054539.1 Arabidopsis thaliana transcription factor EREBP-like protein (At3g14230; MLN21.1) mRNA, complete cds.
GenBankBT0003740.0BT000374.1 Arabidopsis thaliana transcription factor EREBP-like protein (At3g14230) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_850582.10.0related to AP2 2
SwissprotQ9LUM40.0RAP22_ARATH; Ethylene-responsive transcription factor RAP2-2
TrEMBLA0A178VM980.0A0A178VM98_ARATH; RAP2.2
STRINGAT3G14230.10.0(Arabidopsis thaliana)
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
  2. Haas BJ, et al.
    Full-length messenger RNA sequences greatly improve genome annotation.
    Genome Biol., 2002. 3(6): p. RESEARCH0029
  3. Zik M,Irish VF
    Global identification of target genes regulated by APETALA3 and PISTILLATA floral homeotic gene action.
    Plant Cell, 2003. 15(1): p. 207-22
  4. Hirai MY, et al.
    Global expression profiling of sulfur-starved Arabidopsis by DNA macroarray reveals the role of O-acetyl-l-serine as a general regulator of gene expression in response to sulfur nutrition.
    Plant J., 2003. 33(4): p. 651-63
  5. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
  6. Hu W,Wang Y,Bowers C,Ma H
    Isolation, sequence analysis, and expression studies of florally expressed cDNAs in Arabidopsis.
    Plant Mol. Biol., 2003. 53(4): p. 545-63
  7. Nakayama N, et al.
    Gene trap lines define domains of gene regulation in Arabidopsis petals and stamens.
    Plant Cell, 2005. 17(9): p. 2486-506
  8. Nakano T,Suzuki K,Fujimura T,Shinshi H
    Genome-wide analysis of the ERF gene family in Arabidopsis and rice.
    Plant Physiol., 2006. 140(2): p. 411-32
  9. Mandaokar A, et al.
    Transcriptional regulators of stamen development in Arabidopsis identified by transcriptional profiling.
    Plant J., 2006. 46(6): p. 984-1008
  10. Brodersen P, et al.
    Arabidopsis MAP kinase 4 regulates salicylic acid- and jasmonic acid/ethylene-dependent responses via EDS1 and PAD4.
    Plant J., 2006. 47(4): p. 532-46
  11. Welsch R,Maass D,Voegel T,Dellapenna D,Beyer P
    Transcription factor RAP2.2 and its interacting partner SINAT2: stable elements in the carotenogenesis of Arabidopsis leaves.
    Plant Physiol., 2007. 145(3): p. 1073-85
  12. Ascencio-Ib
    Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection.
    Plant Physiol., 2008. 148(1): p. 436-54
  13. Hinz M, et al.
    Arabidopsis RAP2.2: an ethylene response transcription factor that is important for hypoxia survival.
    Plant Physiol., 2010. 153(2): p. 757-72
  14. Hanada K, et al.
    Functional compensation of primary and secondary metabolites by duplicate genes in Arabidopsis thaliana.
    Mol. Biol. Evol., 2011. 28(1): p. 377-82
  15. Ou B, et al.
    A high-throughput screening system for Arabidopsis transcription factors and its application to Med25-dependent transcriptional regulation.
    Mol Plant, 2011. 4(3): p. 546-55
  16. Zhao Y, et al.
    Arabidopsis RAP2.2 plays an important role in plant resistance to Botrytis cinerea and ethylene responses.
    New Phytol., 2012. 195(2): p. 450-60
  17. Efroni I, et al.
    Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses.
    Dev. Cell, 2013. 24(4): p. 438-45
  18. Lumba S, et al.
    A mesoscale abscisic acid hormone interactome reveals a dynamic signaling landscape in Arabidopsis.
    Dev. Cell, 2014. 29(3): p. 360-72
  19. Papdi C, et al.
    The low oxygen, oxidative and osmotic stress responses synergistically act through the ethylene response factor VII genes RAP2.12, RAP2.2 and RAP2.3.
    Plant J., 2015. 82(5): p. 772-84
  20. Bui LT,Giuntoli B,Kosmacz M,Parlanti S,Licausi F
    Constitutively expressed ERF-VII transcription factors redundantly activate the core anaerobic response in Arabidopsis thaliana.
    Plant Sci., 2015. 236: p. 37-43
  21. Gasch P, et al.
    Redundant ERF-VII Transcription Factors Bind to an Evolutionarily Conserved cis-Motif to Regulate Hypoxia-Responsive Gene Expression in Arabidopsis.
    Plant Cell, 2016. 28(1): p. 160-80
  22. Yao Y, et al.
    ETHYLENE RESPONSE FACTOR 74 (ERF74) plays an essential role in controlling a respiratory burst oxidase homolog D (RbohD)-dependent mechanism in response to different stresses in Arabidopsis.
    New Phytol., 2017. 213(4): p. 1667-1681
  23. Giuntoli B, et al.
    Age-dependent regulation of ERF-VII transcription factor activity in Arabidopsis thaliana.
    Plant Cell Environ., 2017. 40(10): p. 2333-2346
  24. Okamuro JK,Caster B,Villarroel R,Van Montagu M,Jofuku KD
    The AP2 domain of APETALA2 defines a large new family of DNA binding proteins in Arabidopsis.
    Proc. Natl. Acad. Sci. U.S.A., 1997. 94(13): p. 7076-81