PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT2G46270.1
Common NameBZIP55, GBF3, T3F17.8
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family bZIP
Protein Properties Length: 382aa    MW: 41113.8 Da    PI: 9.7129
Description G-box binding factor 3
Gene Model
Gene Model ID Type Source Coding Sequence
AT2G46270.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
       bZIP_1   1 ekelkrerrkqkNReAArrsRqRKkaeieeLeekvkeLeaeNkaLkkeleelkkevaklkse 62 
                  89*******************************************************99865 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PfamPF077771.7E-30196IPR012900G-box binding protein, multifunctional mosaic region
PfamPF165967.3E-16129236No hitNo description
Gene3DG3DSA: hitNo description
PfamPF001702.4E-21257319IPR004827Basic-leucine zipper domain
SMARTSM003381.2E-21257321IPR004827Basic-leucine zipper domain
PROSITE profilePS5021713.403259322IPR004827Basic-leucine zipper domain
SuperFamilySSF579595.88E-11260316No hitNo description
CDDcd147028.06E-23262313No hitNo description
PROSITE patternPS000360264279IPR004827Basic-leucine zipper domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009737Biological Processresponse to abscisic acid
GO:0005634Cellular Componentnucleus
GO:0005829Cellular Componentcytosol
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0043565Molecular Functionsequence-specific DNA binding
GO:0044212Molecular Functiontranscription regulatory region DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000084anatomyplant sperm cell
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009009anatomyplant embryo
PO:0009025anatomyvascular leaf
PO:0009052anatomyflower pedicel
PO:0025022anatomycollective leaf structure
PO:0025195anatomypollen tube cell
PO:0001016developmental stageL mature pollen stage
PO:0001017developmental stageM germinated pollen stage
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 382 aa     Download sequence    Send to blast
Nucleic Localization Signal ? help Back to Top
No. Start End Sequence
Expression -- Microarray ? help Back to Top
Source ID E-value
Expression AtlasAT2G46270-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Present only in dark grown leaves and roots. {ECO:0000269|PubMed:8672884}.
Functional Description ? help Back to Top
Source Description
TAIRencodes a bZIP G-box binding protein whose expression is induced by ABA. It has been shown to bind to Adh that contains the G-box and is induced by cold and water deprivation. GBF3 has been shown to be expressed mostly in the root and dark-grown leaves. GBF3 can act as homodimers and as heterodimers with GFB1, GBF2 and GBF4. In addition, GBF3s DNA binding activity is enhanced by GIP1, GPRI1 and GPRI2.
UniProtBinds to the G-box motif (5'-CCACGTGG-3') of the rbcS-1A gene promoter. G-box and G-box-like motifs are cis-acting elements defined in promoters of certain plant genes which are regulated by such diverse stimuli as light-induction or hormone control. {ECO:0000269|PubMed:8672884}.
Function -- GeneRIF ? help Back to Top
  1. Results demonstrate the role of GBF3 in imparting drought tolerance in Arabidopsis thaliana and indicate the conserved role of this gene in drought and other abiotic stress tolerance in several plant species.
    [PMID: 28831141]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
Motif logo
Cis-element ? help Back to Top
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT4G10040(A)
Regulation -- Hormone ? help Back to Top
Source Hormone
AHDabscisic acid
Interaction ? help Back to Top
Source Intact With
IntActSearch P42776
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT2G46270
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAF3703070.0AF370307.1 Arabidopsis thaliana putative G-box binding bZIP transcription factor (At2g46270) mRNA, complete cds.
GenBankAY0630930.0AY063093.1 Arabidopsis thaliana putative G-box binding bZIP transcription factor (At2g46270) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_001323457.10.0G-box binding factor 3
RefseqNP_182150.10.0G-box binding factor 3
SwissprotP427760.0GBF3_ARATH; G-box-binding factor 3
STRINGAT2G46270.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Representative plantOGRP43461123
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
  2. Tamai H,Iwabuchi M,Meshi T
    Arabidopsis GARP transcriptional activators interact with the Pro-rich activation domain shared by G-box-binding bZIP factors.
    Plant Cell Physiol., 2002. 43(1): p. 99-107
  3. Jakoby M, et al.
    bZIP transcription factors in Arabidopsis.
    Trends Plant Sci., 2002. 7(3): p. 106-11
  4. Suzuki M,Ketterling MG,Li QB,McCarty DR
    Viviparous1 alters global gene expression patterns through regulation of abscisic acid signaling.
    Plant Physiol., 2003. 132(3): p. 1664-77
  5. Schindler U,Menkens AE,Beckmann H,Ecker JR,Cashmore AR
    Heterodimerization between light-regulated and ubiquitously expressed Arabidopsis GBF bZIP proteins.
    EMBO J., 1992. 11(4): p. 1261-73
  6. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
  7. Leonhardt N, et al.
    Microarray expression analyses of Arabidopsis guard cells and isolation of a recessive abscisic acid hypersensitive protein phosphatase 2C mutant.
    Plant Cell, 2004. 16(3): p. 596-615
  8. Rizhsky L, et al.
    When defense pathways collide. The response of Arabidopsis to a combination of drought and heat stress.
    Plant Physiol., 2004. 134(4): p. 1683-96
  9. Deppmann CD, et al.
    Dimerization specificity of all 67 B-ZIP motifs in Arabidopsis thaliana: a comparison to Homo sapiens B-ZIP motifs.
    Nucleic Acids Res., 2004. 32(11): p. 3435-45
  10. Guan Y,Nothnagel EA
    Binding of arabinogalactan proteins by Yariv phenylglycoside triggers wound-like responses in Arabidopsis cell cultures.
    Plant Physiol., 2004. 135(3): p. 1346-66
  11. Miao Y,Laun T,Zimmermann P,Zentgraf U
    Targets of the WRKY53 transcription factor and its role during leaf senescence in Arabidopsis.
    Plant Mol. Biol., 2004. 55(6): p. 853-67
  12. Sehnke PC,Laughner BJ,Lyerly Linebarger CR,Gurley WB,Ferl RJ
    Identification and characterization of GIP1, an Arabidopsis thaliana protein that enhances the DNA binding affinity and reduces the oligomeric state of G-box binding factors.
    Cell Res., 2005. 15(8): p. 567-75
  13. Fujita Y, et al.
    AREB1 Is a transcription activator of novel ABRE-dependent ABA signaling that enhances drought stress tolerance in Arabidopsis.
    Plant Cell, 2005. 17(12): p. 3470-88
  14. Deppmann CD,Alvania RS,Taparowsky EJ
    Cross-species annotation of basic leucine zipper factor interactions: Insight into the evolution of closed interaction networks.
    Mol. Biol. Evol., 2006. 23(8): p. 1480-92
  15. Thibaud-Nissen F, et al.
    Development of Arabidopsis whole-genome microarrays and their application to the discovery of binding sites for the TGA2 transcription factor in salicylic acid-treated plants.
    Plant J., 2006. 47(1): p. 152-62
  16. Oravecz A, et al.
    CONSTITUTIVELY PHOTOMORPHOGENIC1 is required for the UV-B response in Arabidopsis.
    Plant Cell, 2006. 18(8): p. 1975-90
  17. Obayashi T, et al.
    ATTED-II: a database of co-expressed genes and cis elements for identifying co-regulated gene groups in Arabidopsis.
    Nucleic Acids Res., 2007. 35(Database issue): p. D863-9
  18. Xin Z,Mandaokar A,Chen J,Last RL,Browse J
    Arabidopsis ESK1 encodes a novel regulator of freezing tolerance.
    Plant J., 2007. 49(5): p. 786-99
  19. Chawade A,Br
    Putative cold acclimation pathways in Arabidopsis thaliana identified by a combined analysis of mRNA co-expression patterns, promoter motifs and transcription factors.
    BMC Genomics, 2007. 8: p. 304
  20. Catala R, et al.
    The Arabidopsis E3 SUMO ligase SIZ1 regulates plant growth and drought responses.
    Plant Cell, 2007. 19(9): p. 2952-66
  21. Shen H,Cao K,Wang X
    AtbZIP16 and AtbZIP68, two new members of GBFs, can interact with other G group bZIPs in Arabidopsis thaliana.
    BMB Rep, 2008. 41(2): p. 132-8
  22. Wohlbach DJ,Quirino BF,Sussman MR
    Analysis of the Arabidopsis histidine kinase ATHK1 reveals a connection between vegetative osmotic stress sensing and seed maturation.
    Plant Cell, 2008. 20(4): p. 1101-17
  23. Wang Y, et al.
    Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis.
    Plant Physiol., 2008. 148(3): p. 1201-11
  24. Welchen E, et al.
    A segment containing a G-box and an ACGT motif confers differential expression characteristics and responses to the Arabidopsis Cytc-2 gene, encoding an isoform of cytochrome c.
    J. Exp. Bot., 2009. 60(3): p. 829-45
  25. Gaudinier A, et al.
    Enhanced Y1H assays for Arabidopsis.
    Nat. Methods, 2011. 8(12): p. 1053-5
  26. Efroni I, et al.
    Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses.
    Dev. Cell, 2013. 24(4): p. 438-45
  27. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
  28. Song L, et al.
    A transcription factor hierarchy defines an environmental stress response network.
    Science, 2017.
  29. Ramegowda V, et al.
    GBF3 transcription factor imparts drought tolerance in Arabidopsis thaliana.
    Sci Rep, 2017. 7(1): p. 9148
  30. Menkens AE,Cashmore AR
    Isolation and characterization of a fourth Arabidopsis thaliana G-box-binding factor, which has similarities to Fos oncoprotein.
    Proc. Natl. Acad. Sci. U.S.A., 1994. 91(7): p. 2522-6
  31. Lu G,Paul AL,McCarty DR,Ferl RJ
    Transcription factor veracity: is GBF3 responsible for ABA-regulated expression of Arabidopsis Adh?
    Plant Cell, 1996. 8(5): p. 847-57
  32. Terzaghi WB,Bertekap RL,Cashmore AR
    Intracellular localization of GBF proteins and blue light-induced import of GBF2 fusion proteins into the nucleus of cultured Arabidopsis and soybean cells.
    Plant J., 1997. 11(5): p. 967-82