PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID XP_016493409.1
Organism
Nicotiana tabacum
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae; Nicotiana
Family ARF
Protein Properties Length: 841aa    MW: 94125.8 Da    PI: 6.3182
Description ARF family protein
Gene Model
Gene Model ID Type Source Coding Sequence
XP_016493409.1genomeNCBIView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1B372.64.7e-23129230199
                     EEEE-..-HHHHTT-EE--HHH.HTT.......---..--SEEEEEETTS-EEEEEE..EEETTEEEE-TTHHHHHHHHT--TT-EEEEEE-SSS CS
              B3   1 ffkvltpsdvlksgrlvlpkkfaeeh.......ggkkeesktltledesgrsWevkliyrkksgryvltkGWkeFvkangLkegDfvvFkldgrs 88 
                     f+k+lt sd++++g +++p++ ae+        +   +  ++l+ +d++  +W++++i+r++++r++lt+GW+ Fv+a++L +gD+v+F   +++
  XP_016493409.1 129 FCKTLTASDTSTHGGFSVPRRAAEKVfppldfsQT--PPAQELIARDLHDVEWKFRHIFRGQPKRHLLTTGWSVFVSAKRLVAGDSVLFI--WNE 219
                     99*****************************7444..44459************************************************..888 PP

                     EE..EEEEE-S CS
              B3  89 efelvvkvfrk 99 
                     + +l+++++r+
  XP_016493409.1 220 KNQLLLGIRRA 230
                     9999***9986 PP
2Auxin_resp117.51.1e-38255338183
      Auxin_resp   1 aahaastksvFevvYnPrastseFvvkvekvekalk.vkvsvGmRfkmafetedsserrlsGtvvgvsdldpvrWpnSkWrsLk 83 
                     aahaa+t+s F+v++nPras+seFv++++k+ ka++ ++vsvGmRf+m fete+ss rr++Gt++g+ dldpvrWpnS+Wrs+k
  XP_016493409.1 255 AAHAAATNSCFTVFFNPRASPSEFVIPLSKYIKAVYhTRVSVGMRFRMLFETEESSVRRYMGTITGIGDLDPVRWPNSHWRSVK 338
                     79*********************************9789******************************************985 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF1019364.19E-49113258IPR015300DNA-binding pseudobarrel domain
Gene3DG3DSA:2.40.330.101.1E-40122244IPR015300DNA-binding pseudobarrel domain
CDDcd100172.58E-21128229No hitNo description
PfamPF023622.9E-21129230IPR003340B3 DNA binding domain
PROSITE profilePS5086311.998129231IPR003340B3 DNA binding domain
SMARTSM010191.0E-23129231IPR003340B3 DNA binding domain
PfamPF065075.2E-34255338IPR010525Auxin response factor
PfamPF023098.9E-8706800IPR033389AUX/IAA domain
PROSITE profilePS5174520.753714798IPR000270PB1 domain
SuperFamilySSF542771.57E-6715788No hitNo description
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009725Biological Processresponse to hormone
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0005515Molecular Functionprotein binding
Sequence ? help Back to Top
Protein Sequence    Length: 841 aa     Download sequence    Send to blast
MKLSTSGMGQ QAHEGEKKCL NSELWHACAG PLVCLPTVGS RVVYFPQGHS EQCQVAATTN  60
KEVDAHIPNY PNLPPQLICQ LHNVTMHADV ETDEVYAQMT LQPLTPEEQK DTYLPVEFGI  120
PSKQPTNYFC KTLTASDTST HGGFSVPRRA AEKVFPPLDF SQTPPAQELI ARDLHDVEWK  180
FRHIFRGQPK RHLLTTGWSV FVSAKRLVAG DSVLFIWNEK NQLLLGIRRA IRPQTVMPSS  240
VLSSDSMHIG LLAAAAHAAA TNSCFTVFFN PRASPSEFVI PLSKYIKAVY HTRVSVGMRF  300
RMLFETEESS VRRYMGTITG IGDLDPVRWP NSHWRSVKVG WDESTAGERQ PRVSLWEIEP  360
LTTFPMYPSL FPLRLKRPLY PGTSSYQDSN NEAINRMAWL RGNSGELGPH SVNLQSFGML  420
PWMQQRVDST ILPNDLNQHY QAMLTTGLQS YGSGDLLKQQ LMQFQQPVQY LQHASSDNSI  480
LQQQQVMQQA VHQHMLPAQT QMLSENLQRQ QQQQPNNQSE EQAHQHAYQE AFQIPHDQLQ  540
QRQQPPNIPS SFSKEDFADL NSKFSASVAP SGVQNMLGSF CSEGSSNSLN INRTGQSVVI  600
EQPSQQSWIS KFMQSQLNTC SNSSPLPTYG KDTLNSRENC SLDTQNQALF GASIDSSGLL  660
LPITVSNVTT TCADISSMPL GMNSGYQNSL YGYVQDSSEL LHNAGQNDPP NASHSFVKVY  720
KSGCVGRSLD ITQFHSYHEL RRELGQMFGI EGFLEDPQRS GWQLVFVDRE NDILLLGDDP  780
WEAFVNNVWY IKILSPEDVQ KLGKEEAKSL NRGAVERMSS STSADDRDLV SGMPSLGSLE  840
Y
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
4ldu_A1e-1591635947388Auxin response factor 5
Search in ModeBase
Functional Description ? help Back to Top
Source Description
UniProtAuxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Seems to act as transcriptional activator. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Regulates both stamen and gynoecium maturation. Promotes jasmonic acid production. Partially redundant with ARF6. Involved in fruit initiation. Acts as an inhibitor to stop further carpel development in the absence of fertilization and the generation of signals required to initiate fruit and seed development. {ECO:0000269|PubMed:12036261, ECO:0000269|PubMed:16107481, ECO:0000269|PubMed:16829592}.
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Repressed by miR167. {ECO:0000269|PubMed:17021043}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_016493409.10.0PREDICTED: auxin response factor 8-like isoform X1
SwissprotQ9FGV10.0ARFH_ARATH; Auxin response factor 8
TrEMBLA0A1S4BXA90.0A0A1S4BXA9_TOBAC; Auxin response factor
STRINGXP_009631563.10.0(Nicotiana tomentosiformis)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
AsteridsOGEA38572339
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT5G37020.10.0auxin response factor 8
Publications ? help Back to Top
  1. Liu N, et al.
    Down-regulation of AUXIN RESPONSE FACTORS 6 and 8 by microRNA 167 leads to floral development defects and female sterility in tomato.
    J. Exp. Bot., 2014. 65(9): p. 2507-20
    [PMID:24723401]
  2. Minato N, et al.
    The phytoplasmal virulence factor TENGU causes plant sterility by downregulating of the jasmonic acid and auxin pathways.
    Sci Rep, 2014. 4: p. 7399
    [PMID:25492247]
  3. Ortega-Amaro MA, et al.
    Overexpression of AtGRDP2, a novel glycine-rich domain protein, accelerates plant growth and improves stress tolerance.
    Front Plant Sci, 2014. 5: p. 782
    [PMID:25653657]
  4. Wang Y, et al.
    MicroRNA167-Directed Regulation of the Auxin Response Factors GmARF8a and GmARF8b Is Required for Soybean Nodulation and Lateral Root Development.
    Plant Physiol., 2015. 168(3): p. 984-99
    [PMID:25941314]
  5. Barik S, et al.
    Coevolution Pattern and Functional Conservation or Divergence of miR167s and their targets across Diverse Plant Species.
    Sci Rep, 2015. 5: p. 14611
    [PMID:26459056]
  6. Pashkovskiy PP,Kartashov AV,Zlobin IE,Pogosyan SI,Kuznetsov VV
    Blue light alters miR167 expression and microRNA-targeted auxin response factor genes in Arabidopsis thaliana plants.
    Plant Physiol. Biochem., 2016. 104: p. 146-54
    [PMID:27031426]
  7. Mlotshwa S,Pruss GJ,MacArthur JL,Reed JW,Vance V
    Developmental Defects Mediated by the P1/HC-Pro Potyviral Silencing Suppressor Are Not Due to Misregulation of AUXIN RESPONSE FACTOR 8.
    Plant Physiol., 2016. 172(3): p. 1853-1861
    [PMID:27688620]
  8. Wójcikowska B,Gaj MD
    Expression profiling of AUXIN RESPONSE FACTOR genes during somatic embryogenesis induction in Arabidopsis.
    Plant Cell Rep., 2017. 36(6): p. 843-858
    [PMID:28255787]
  9. Zhang GZ, et al.
    Ectopic expression of UGT84A2 delayed flowering by indole-3-butyric acid-mediated transcriptional repression of ARF6 and ARF8 genes in Arabidopsis.
    Plant Cell Rep., 2017. 36(12): p. 1995-2006
    [PMID:29027578]
  10. Zheng K, et al.
    Involvement of PACLOBUTRAZOL RESISTANCE6/KIDARI, an Atypical bHLH Transcription Factor, in Auxin Responses in Arabidopsis.
    Front Plant Sci, 2017. 8: p. 1813
    [PMID:29114256]
  11. Liu Z, et al.
    ARF2-ARF4 and ARF5 are Essential for Female and Male Gametophyte Development in Arabidopsis.
    Plant Cell Physiol., 2018. 59(1): p. 179-189
    [PMID:29145642]
  12. Ghelli R, et al.
    A Newly Identified Flower-Specific Splice Variant of AUXIN RESPONSE FACTOR8 Regulates Stamen Elongation and Endothecium Lignification in Arabidopsis.
    Plant Cell, 2018. 30(3): p. 620-637
    [PMID:29514943]