PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica

Family

WRKY

Protein Properties

Length: 292aa MW: 33016.9 Da PI: 5.514

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
XP_013637136.1	genome	NCBI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	87.5	1.2e-27	100	160	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-ST...T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsa...gCpvkkkversaedpkvveitYegeHnhe 59 dDgy+WrKYGqKe++gs +pr+Y+rCt++ +C + k+v++s++dp+++e++Y g+H+++ XP_013637136.1 100 DDGYCWRKYGQKEIHGSINPRGYFRCTHRftqNCLAVKQVQKSDRDPSIFEVKYVGSHTCN 160 8*************************9999*************************97 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	1.2E-25	92	161	IPR003657	WRKY domain
PROSITE profile	PS50811	21.138	94	162	IPR003657	WRKY domain
SuperFamily	SSF118290	1.12E-23	97	160	IPR003657	WRKY domain
SMART	SM00774	9.5E-35	99	161	IPR003657	WRKY domain
Pfam	PF03106	8.6E-23	100	159	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0010200	Biological Process	response to chitin
GO:0048527	Biological Process	lateral root development
GO:0005730	Cellular Component	nucleolus
GO:0000987	Molecular Function	core promoter proximal region sequence-specific DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 292 aa Download sequence Send to blast
MVMEEKLVIN ELEQGRELAK RLMSNLKDTS SIESSKSLIS EILSIYQNAI SMLDDKKVLK 60 RSREIDDKYS KNVIKKRKVF EKTEKVSFFV GAGQEKGSID DGYCWRKYGQ KEIHGSINPR 120 GYFRCTHRFT QNCLAVKQVQ KSDRDPSIFE VKYVGSHTCN NTTTSPKTPN FSVSTFQEGN 180 TADEQREDIK PTKTEEAMMS FEDLESKKNI FRTFSFSNYE FENGGGGWKG NLFHENQLSP 240 AATTSGSGIT SEIATAPASV ENSETADSYF SSLDNIIDFG PDWLLSCDVL NW

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
6ir8_A	2e-21	98	159	7	68	OsWRKY45
Search in ModeBase

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Expressed in guard cells, hypocotyls, and in the vascular tissues of cotyledon and root (PubMed:24773321). Mostly expressed in roots, at lower levels in leaves and petioles, and, to a lower extent, in stems, flowers and siliques (PubMed:22325892). {ECO:0000269\|PubMed:22325892, ECO:0000269\|PubMed:24773321}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor involved in the regulation of osmotic stress responses and stomatal movement (PubMed:24773321). Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). Positive regulator of EDS1-dependent defense against E.amylovora (PubMed:22316300). Together with WRKY70 and WRKY53, promotes resistance to P.syringae, probably by enhancing salicylic acid (SA)- dependent genes. Contributes to the suppression of jasmonic acid (MeJA)-induced expression of PDF1.2 (PubMed:22325892). Together with WRKY54 and WRKY70, promotes brassinosteroid (BR)-regulated plant growth but prevent drought response by modulating gene expression (PubMed:28576847). {ECO:0000250, ECO:0000269\|PubMed:22316300, ECO:0000269\|PubMed:22325892, ECO:0000269\|PubMed:24773321, ECO:0000269\|PubMed:28576847}.

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00112	ampDAP	Transfer from AT2G46400	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	XP_013637136.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Up-regulated by drought, salt and hydrogen peroxide treatments (PubMed:24773321). Induced by salicylic acid (SA) (PubMed:22268143, PubMed:22325892). Up-regulated by E.amylovora (PubMed:22316300). Triggered by P.syringae (PubMed:22325892). {ECO:0000269\|PubMed:22268143, ECO:0000269\|PubMed:22316300, ECO:0000269\|PubMed:22325892, ECO:0000269\|PubMed:24773321}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	HM585284	0.0	HM585284.1 Brassica rapa subsp. chinensis WRKY46 mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_013637136.1	0.0	PREDICTED: probable WRKY transcription factor 46
Swissprot	Q9SKD9	1e-141	WRK46_ARATH; Probable WRKY transcription factor 46
TrEMBL	A0A0D3BNT8	0.0	A0A0D3BNT8_BRAOL; Uncharacterized protein
STRING	Bo4g009270.1	0.0	(Brassica oleracea)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM4888	23	52

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT2G46400.1	1e-134	WRKY DNA-binding protein 46

Publications ? help Back to Top
Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Sheikh AH, et al. Regulation of WRKY46 Transcription Factor Function by Mitogen-Activated Protein Kinases in Arabidopsis thaliana. Front Plant Sci, 2016. 7: p. 61 [PMID:26870073] Yan JY, et al. A WRKY Transcription Factor Regulates Fe Translocation under Fe Deficiency. Plant Physiol., 2016. 171(3): p. 2017-27 [PMID:27208259] Chen J, et al. Arabidopsis WRKY46, WRKY54, and WRKY70 Transcription Factors Are Involved in Brassinosteroid-Regulated Plant Growth and Drought Responses. Plant Cell, 2017. 29(6): p. 1425-1439 [PMID:28576847] Ma J, et al. Overexpression of TaWRKY146 Increases Drought Tolerance through Inducing Stomatal Closure in Arabidopsis thaliana. Front Plant Sci, 2017. 8: p. 2036 [PMID:29225611]