PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae; Nicotiana

Family

WRKY

Protein Properties

Length: 303aa MW: 34203.9 Da PI: 6.0683

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
XP_009595576.1	genome	NCBI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	96.9	1.4e-30	78	136	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 +D ++WrKYGqK++kgs++pr+YYrC+s+ gCp++k+vers+ dp+++++tY+ eHnh+ XP_009595576.1 78 SDSWAWRKYGQKPIKGSPYPRGYYRCSSSkGCPARKQVERSRADPNMLVVTYSCEHNHP 136 69***********************998**************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	1.8E-31	74	138	IPR003657	WRKY domain
SuperFamily	SSF118290	2.35E-26	76	138	IPR003657	WRKY domain
SMART	SM00774	4.2E-36	77	137	IPR003657	WRKY domain
PROSITE profile	PS50811	32.409	78	138	IPR003657	WRKY domain
Pfam	PF03106	3.8E-26	79	136	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 303 aa Download sequence Send to blast
MDGRFNNIFV SHEQEDSENS PENSLDSPRS DMFNDNKMMT STSSPKRSRR SIQKRVVSVP 60 IKEVEGTKMK GEISMPPSDS WAWRKYGQKP IKGSPYPRGY YRCSSSKGCP ARKQVERSRA 120 DPNMLVVTYS CEHNHPWPAS RNNQHNQRTT TTSCINNAKT KTKTLASLTA TTTIITTTNI 180 AVSDFERKKD TSNFASQSEP NSDENFTNLG ESSLISCNEF GWFSDFECTS TTMLESPILT 240 EVEVTDIDMS STLTTMREED DSLFADLGEL PECSRVFGRG MMERDEERRR HSLSLTSWCG 300 TLG

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
5w3x_B	8e-22	67	138	3	79	Disease resistance protein RRS1
5w3x_D	8e-22	67	138	3	79	Disease resistance protein RRS1
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). {ECO:0000250}.

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00167	DAP	Transfer from AT1G29280	Download

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	DQ102364	0.0	DQ102364.1 Capsicum annuum WRKY-A1244 mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_009595576.1	0.0	PREDICTED: probable WRKY transcription factor 65 isoform X1
Swissprot	Q9LP56	7e-76	WRK65_ARATH; Probable WRKY transcription factor 65
TrEMBL	A0A1S4DEE6	0.0	A0A1S4DEE6_TOBAC; probable WRKY transcription factor 65 isoform X1
STRING	XP_009595576.1	0.0	(Nicotiana tomentosiformis)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Asterids	OGEA6475	23	33

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT1G29280.1	9e-68	WRKY DNA-binding protein 65

Publications ? help Back to Top
Duarte JM, et al. Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. Mol. Biol. Evol., 2006. 23(2): p. 469-78 [PMID:16280546] Singh V,Roy S,Singh D,Nandi AK Arabidopsis flowering locus D influences systemic-acquired-resistance- induced expression and histone modifications of WRKY genes. J. Biosci., 2014. 39(1): p. 119-26 [PMID:24499796]