PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID XP_009587041.1
Organism
Nicotiana tomentosiformis
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae; Nicotiana
Family bHLH
Protein Properties Length: 444aa    MW: 49719.6 Da    PI: 5.7858
Description bHLH family protein
Gene Model
Gene Model ID Type Source Coding Sequence
XP_009587041.1genomeNCBIView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HLH28.43e-09249296455
                     HHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS
             HLH   4 ahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 
                     +h+ +Er RR++i +++  L++l+P +    +k   Ka +L + ++Y++sLq
  XP_009587041.1 249 SHSLAERVRREKISERMRFLQDLVPGC----NKITGKAGMLDEIINYVQSLQ 296
                     8**************************....999*****************9 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF474591.57E-16243313IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
CDDcd000832.48E-10243300No hitNo description
Gene3DG3DSA:4.10.280.101.6E-16245312IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PROSITE profilePS5088815.485245295IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PfamPF000101.5E-6249296IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SMARTSM003531.4E-8251301IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006351Biological Processtranscription, DNA-templated
GO:0009637Biological Processresponse to blue light
GO:0009911Biological Processpositive regulation of flower development
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0046983Molecular Functionprotein dimerization activity
Sequence ? help Back to Top
Protein Sequence    Length: 444 aa     Download sequence    Send to blast
MNIALPEMLH SITGNGGNGS SEMSVLERQR ARMKWQQEQL QQQEMMSYFD GQNDQQLMNF  60
YSQTAGAQQF HGQINNVNDE SLNELVTRAI KPDPCLENNW TIGTDGLGYV PTGVGYGGTT  120
PQLGLNYAIS RTTSCPPNMA DNAVSDGKAK ESMMSSNRGR ESFKKRKADK NQHLKELAEE  180
ETKDKKMKEC IEEGDSKVTT EKYNNRRSTN NSNNSKESSE SSKEKSKITE DKKPDYIHVR  240
ARRGQATDSH SLAERVRREK ISERMRFLQD LVPGCNKITG KAGMLDEIIN YVQSLQRQVE  300
FLSMKLAAVN PRLDIEIDNF FNKDIFATST ANFPTVGAGT SSEMLSMAQR QFNSLQQIVS  360
SSGFEMGILN LSEMALRRTT SAPVSIPEIF LDSSNINQVQ SFPTWDTDLD NMYAMDLQQG  420
RSAQFLPHPS TGFVEAGNDL KMGM
Functional Description ? help Back to Top
Source Description
UniProtTranscription factor that binds DNA to G box 5'-CACGTG-3' and, to a lower extent, to E-box 5'-CANNTG-3' in vitro. Binds to chromatin DNA of the FT gene and promotes its expression, and thus triggers flowering in response to blue light. {ECO:0000269|PubMed:18988809, ECO:0000269|PubMed:24130508}.
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Accumulates strongly in response to blue light due to reduced preventing 26S proteasome-mediated degradation in an ADO1/ZTL and ADO2/LKP2 dependent manner, but levels decrease in the absence of blue light via 26S proteasome degradation (at protein level). {ECO:0000269|PubMed:24101505}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankBT0143000.0BT014300.1 Lycopersicon esculentum clone 133550R, mRNA sequence.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_009587041.10.0PREDICTED: transcription factor bHLH63-like
RefseqXP_016441121.10.0PREDICTED: transcription factor bHLH63-like
SwissprotQ8GY612e-58BH063_ARATH; Transcription factor bHLH63
TrEMBLA0A1S3XMF20.0A0A1S3XMF2_TOBAC; transcription factor bHLH63-like
STRINGXP_009587041.10.0(Nicotiana tomentosiformis)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
AsteridsOGEA65382330
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT4G34530.12e-55cryptochrome-interacting basic-helix-loop-helix 1
Publications ? help Back to Top
  1. Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH
    Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature.
    Differentiation, 2010. 80(1): p. 1-8
    [PMID:20219281]
  2. Yang D,Zhao W,Meng Y,Li H,Liu B
    A CIB1-LIKE transcription factor GmCIL10 from soybean positively regulates plant flowering.
    Sci China Life Sci, 2015. 58(3): p. 261-9
    [PMID:25651969]
  3. Katsura Y, et al.
    An optogenetic system for interrogating the temporal dynamics of Akt.
    Sci Rep, 2015. 5: p. 14589
    [PMID:26423353]
  4. Taslimi A, et al.
    Optimized second-generation CRY2-CIB dimerizers and photoactivatable Cre recombinase.
    Nat. Chem. Biol., 2016. 12(6): p. 425-30
    [PMID:27065233]
  5. Liu Q, et al.
    The Blue Light-Dependent Polyubiquitination and Degradation of Arabidopsis Cryptochrome2 Requires Multiple E3 Ubiquitin Ligases.
    Plant Cell Physiol., 2016. 57(10): p. 2175-2186
    [PMID:27516416]
  6. Pathak GP, et al.
    Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2.
    Nucleic Acids Res., 2017. 45(20): p. e167
    [PMID:28431041]
  7. Duan L, et al.
    Understanding CRY2 interactions for optical control of intracellular signaling.
    Nat Commun, 2017. 8(1): p. 547
    [PMID:28916751]
  8. Quejada JR, et al.
    Optimized light-inducible transcription in mammalian cells using Flavin Kelch-repeat F-box1/GIGANTEA and CRY2/CIB1.
    Nucleic Acids Res., 2017. 45(20): p. e172
    [PMID:29040770]
  9. Liu Y, et al.
    CIB1 and CO interact to mediate CRY2-dependent regulation of flowering.
    EMBO Rep., 2019.
    [PMID:30126927]