PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID XP_009113574.1
Common NameTT8
Organism
Brassica rapa
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica
Family bHLH
Protein Properties Length: 521aa    MW: 60014.1 Da    PI: 5.6146
Description bHLH family protein
Gene Model
Gene Model ID Type Source Coding Sequence
XP_009113574.1genomeNCBIView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HLH43.46.2e-14367412454
                     HHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHH CS
             HLH   4 ahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksL 54 
                     +h  +ErrRR+++N++f +Lr+l+P       +K++K++iL  ++eY+++L
  XP_009113574.1 367 NHVVAERRRREKLNERFITLRSLVPFV-----TKMDKVSILGDTIEYVNHL 412
                     7999**********************8.....8*****************9 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PfamPF142152.1E-5121201IPR025610Transcription factor MYC/MYB N-terminal
PROSITE profilePS5088817.295363412IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamilySSF474592.36E-18366429IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
CDDcd000831.04E-13366417No hitNo description
PfamPF000102.8E-11367412IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene3DG3DSA:4.10.280.103.4E-19367431IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SMARTSM003536.1E-17369418IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0010026Biological Processtrichome differentiation
GO:0010214Biological Processseed coat development
GO:2000029Biological Processregulation of proanthocyanidin biosynthetic process
GO:0000976Molecular Functiontranscription regulatory region sequence-specific DNA binding
GO:0046983Molecular Functionprotein dimerization activity
Sequence ? help Back to Top
Protein Sequence    Length: 521 aa     Download sequence    Send to blast
MDELSIIPLW KVIGAEKEEI QGLLKAVVQS VGWTYSVFWQ LCPQRRKLMW SSGYYNGAIK  60
TRKTTQPAEV TAEEAASERS QQLMELYETL FAGESSMEAR ACTALSPEDL TDPEWFYVLC  120
FTYSFEPPSG MPGKAYARRK HIWLRGANEV DNKIFSRAIS AKSAKIQTVV CIPVLDGVLE  180
LGTTNKVKES EEFVEHIKSF FHNHPKSNTK PTLSEHFINE EHEEDEEEVE EEEMTMSEEI  240
RLGSPDDDDV SNQNLLSDFH IEATNSLDTH MDMMNLMEEG GNYSQTVSTL LMSQPTSLLS  300
DSVSTSSYVQ SSFVSWRVEN VKEHQQYQRV EKAAWSSSQW MLKHIILKVP FLHDNTKNKR  360
LPREELNHVV AERRRREKLN ERFITLRSLV PFVTKMDKVS ILGDTIEYVN HLSKRIHELE  420
STHHEPNQKR MRIGKGRTWE EVEVSIIESD VLLEMRCEYR DGLLLNILQV LKELGIETTA  480
VHTALNDNHF EAEIRAKVRG KKPTIAEVKI AIHQIIYNNK L
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
4rqw_A2e-19172026193Transcription factor MYC3
4rqw_B2e-19172026193Transcription factor MYC3
4rs9_A2e-19172026193Transcription factor MYC3
4yz6_A2e-19172026193Transcription factor MYC3
Search in ModeBase
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
1371376ERRRRE
2372377RRRREK
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: Faint expression in buds and flowers. Increases during the very early stages of seed development, reaches a maximum at the globular embryo stage and stays high throughout seed formation.
UniprotTISSUE SPECIFICITY: Buds, flowers and developing siliques, but not in leaves, stems and roots. {ECO:0000269|PubMed:12679534}.
Functional Description ? help Back to Top
Source Description
UniProtTranscription activator, when associated with MYB75/PAP1 or MYB90/PAP2. Involved in the control of flavonoid pigmentation. Plays a key role in regulating leucoanthocyanidin reductase (BANYULS) and dihydroflavonol-4-reductase (DFR). Not required for leucoanthocyanidin dioxygenase (LDOX) expression. {ECO:0000269|PubMed:15361138}.
Cis-element ? help Back to Top
SourceLink
PlantRegMapXP_009113574.1
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankEU1920270.0EU192027.1 Brassica napus bHLH DNA-binding domain transcription factor (TT8.1) mRNA, complete cds.
GenBankHQ3377910.0HQ337791.1 Brassica rapa subsp. rapa cultivar Tsuda transparent testa 8 (TT8) mRNA, complete cds.
GenBankKM4034520.0KM403452.1 Brassica rapa cultivar Zi He bHLH transcription factor (TT8) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_009113574.10.0PREDICTED: transcription factor TT8
SwissprotQ9FT810.0TT8_ARATH; Transcription factor TT8
TrEMBLG8Z8Z30.0G8Z8Z3_BRACM; BHLH DNA binding domain transcription factor BraA.TT8
STRINGBo9g086910.10.0(Brassica oleracea)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM94222836
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT4G09820.10.0bHLH family protein
Publications ? help Back to Top
  1. Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH
    Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature.
    Differentiation, 2010. 80(1): p. 1-8
    [PMID:20219281]
  2. Kim K,Jiang K,Teng SL,Feldman LJ,Huang H
    Using biologically interrelated experiments to identify pathway genes in Arabidopsis.
    Bioinformatics, 2012. 28(6): p. 815-22
    [PMID:22271267]
  3. Wang Y,Zhou B,Sun M,Li Y,Kawabata S
    UV-A light induces anthocyanin biosynthesis in a manner distinct from synergistic blue + UV-B light and UV-A/blue light responses in different parts of the hypocotyls in turnip seedlings.
    Plant Cell Physiol., 2012. 53(8): p. 1470-80
    [PMID:22706033]
  4. Liu Z,Shi MZ,Xie DY
    Regulation of anthocyanin biosynthesis in Arabidopsis thaliana red pap1-D cells metabolically programmed by auxins.
    Planta, 2014. 239(4): p. 765-81
    [PMID:24370633]
  5. Ranocha P,Francoz E,Burlat V,Dunand C
    Expression of PRX36, PMEI6 and SBT1.7 is controlled by complex transcription factor regulatory networks for proper seed coat mucilage extrusion.
    Plant Signal Behav, 2014. 9(11): p. e977734
    [PMID:25531128]
  6. Zhu Z, et al.
    Characterization of the cis elements in the proximal promoter regions of the anthocyanin pathway genes reveals a common regulatory logic that governs pathway regulation.
    J. Exp. Bot., 2015. 66(13): p. 3775-89
    [PMID:25911741]
  7. Zhu Q, et al.
    Ectopic Expression of the Coleus R2R3 MYB-Type Proanthocyanidin Regulator Gene SsMYB3 Alters the Flower Color in Transgenic Tobacco.
    PLoS ONE, 2015. 10(10): p. e0139392
    [PMID:26448466]
  8. Li P, et al.
    Regulation of anthocyanin and proanthocyanidin biosynthesis by Medicago truncatula bHLH transcription factor MtTT8.
    New Phytol., 2016. 210(3): p. 905-21
    [PMID:26725247]
  9. Bulgakov VP,Veremeichik GN,Grigorchuk VP,Rybin VG,Shkryl YN
    The rolB gene activates secondary metabolism in Arabidopsis calli via selective activation of genes encoding MYB and bHLH transcription factors.
    Plant Physiol. Biochem., 2016. 102: p. 70-9
    [PMID:26913794]
  10. Petridis A,Döll S,Nichelmann L,Bilger W,Mock HP
    Arabidopsis thaliana G2-LIKE FLAVONOID REGULATOR and BRASSINOSTEROID ENHANCED EXPRESSION1 are low-temperature regulators of flavonoid accumulation.
    New Phytol., 2016. 211(3): p. 912-25
    [PMID:27125220]
  11. Rai A, et al.
    Coordinate Regulation of Metabolite Glycosylation and Stress Hormone Biosynthesis by TT8 in Arabidopsis.
    Plant Physiol., 2016. 171(4): p. 2499-515
    [PMID:27432888]
  12. Li Y, et al.
    Two IIIf Clade-bHLHs from Freesia hybrida Play Divergent Roles in Flavonoid Biosynthesis and Trichome Formation when Ectopically Expressed in Arabidopsis.
    Sci Rep, 2016. 6: p. 30514
    [PMID:27465838]
  13. Mahmood K,Xu Z,El-Kereamy A,Casaretto JA,Rothstein SJ
    The Arabidopsis Transcription Factor ANAC032 Represses Anthocyanin Biosynthesis in Response to High Sucrose and Oxidative and Abiotic Stresses.
    Front Plant Sci, 2016. 7: p. 1548
    [PMID:27790239]
  14. Zou B, et al.
    Calmodulin-binding protein CBP60g functions as a negative regulator in Arabidopsis anthocyanin accumulation.
    PLoS ONE, 2017. 12(3): p. e0173129
    [PMID:28253311]
  15. Chen K,Liu H,Lou Q,Liu Y
    Ectopic Expression of the Grape Hyacinth (Muscari armeniacum) R2R3-MYB Transcription Factor Gene, MaAN2, Induces Anthocyanin Accumulation in Tobacco.
    Front Plant Sci, 2017. 8: p. 965
    [PMID:28642775]
  16. Kataya ARA, et al.
    PLATINUM SENSITIVE 2 LIKE impacts growth, root morphology, seed set, and stress responses.
    PLoS ONE, 2017. 12(7): p. e0180478
    [PMID:28678890]
  17. Ezer D, et al.
    The G-Box Transcriptional Regulatory Code in Arabidopsis.
    Plant Physiol., 2017. 175(2): p. 628-640
    [PMID:28864470]
  18. Feng K, et al.
    An R2R3-MYB transcription factor, OjMYB1, functions in anthocyanin biosynthesis in Oenanthe javanica.
    Planta, 2018. 247(2): p. 301-315
    [PMID:28965159]
  19. Wen J, et al.
    The C-terminal domains of Arabidopsis GL3/EGL3/TT8 interact with JAZ proteins and mediate dimeric interactions.
    Plant Signal Behav, 2018. 13(1): p. e1422460
    [PMID:29293407]