PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; Cicereae; Cicer

Family

WRKY

Protein Properties

Length: 598aa MW: 65465 Da PI: 6.6245

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
XP_004508768.1	genome	NCBI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	94.3	8.7e-30	250	309	1	59
---SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 1 ldDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 ++Dg++WrKYGqK kg+++pr+YYrCt a +Cpv+k+v+r+ +d +++++tYeg+Hnh+ XP_004508768.1 250 MNDGCQWRKYGQKIAKGNPCPRAYYRCTVApSCPVRKQVQRCVDDISILITTYEGTHNHN 309 58*************************99**************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	3.7E-31	236	311	IPR003657	WRKY domain
SuperFamily	SSF118290	4.18E-27	243	311	IPR003657	WRKY domain
PROSITE profile	PS50811	28.139	245	311	IPR003657	WRKY domain
SMART	SM00774	1.6E-35	250	310	IPR003657	WRKY domain
Pfam	PF03106	5.0E-26	251	308	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 598 aa Download sequence Send to blast
MDCNSGRSGD GDSGLKEEKR SEHSNIGDDK SHHHKEIIVE EPPVVNTQRS MVEAGPSASS 60 AKNDKADDEL KTTKAQMSEV REENERLKMC LNKIMNEYRT LEMQFHNMVK QETKKNSVDD 120 NHNKEIIVES DLVSLSLGRV PSSNPRNEVN KVSKLALNNN DEEFKEELSL GLDCKFETSK 180 SGSTNEGLPN ISSPINSSEV PTKEEEGGET WPPSKTLNKM VRDTEDEVVQ QAPPKKARVC 240 VRARCDTPTM NDGCQWRKYG QKIAKGNPCP RAYYRCTVAP SCPVRKQVQR CVDDISILIT 300 TYEGTHNHNL PLSATAMAST TSAAASMLLS GSSTSNSASY SSTPSNNNLH GLNFYLPDGS 360 KSNQLYISNP ALSSQHSHPT ITLDLTSNPS NSSISSPFVR FNSNYNNNNQ ARYPSSTSLN 420 FSSPETNTMH WNNSFLNYAT QNQSYNNRNN NLSTLNFGRP NTMESIYQSY MQKNNNSNIS 480 QHGLQDSTIS AATKAITADP NFQSALAAAL SSLIGNTHQG NQNQSVGENL SQKMKWAEMF 540 PFPMSSSTSL ASSSSKVNGC GSSLLNKTQV DAQMGSFMSL STTKSASASP ADNSDNTD

Sequence ? help Back to Top

Protein Sequence Length: 598 aa Download sequence Send to blast

MDCNSGRSGD GDSGLKEEKR SEHSNIGDDK SHHHKEIIVE EPPVVNTQRS MVEAGPSASS  60
AKNDKADDEL KTTKAQMSEV REENERLKMC LNKIMNEYRT LEMQFHNMVK QETKKNSVDD  120
NHNKEIIVES DLVSLSLGRV PSSNPRNEVN KVSKLALNNN DEEFKEELSL GLDCKFETSK  180
SGSTNEGLPN ISSPINSSEV PTKEEEGGET WPPSKTLNKM VRDTEDEVVQ QAPPKKARVC  240
VRARCDTPTM NDGCQWRKYG QKIAKGNPCP RAYYRCTVAP SCPVRKQVQR CVDDISILIT  300
TYEGTHNHNL PLSATAMAST TSAAASMLLS GSSTSNSASY SSTPSNNNLH GLNFYLPDGS  360
KSNQLYISNP ALSSQHSHPT ITLDLTSNPS NSSISSPFVR FNSNYNNNNQ ARYPSSTSLN  420
FSSPETNTMH WNNSFLNYAT QNQSYNNRNN NLSTLNFGRP NTMESIYQSY MQKNNNSNIS  480
QHGLQDSTIS AATKAITADP NFQSALAAAL SSLIGNTHQG NQNQSVGENL SQKMKWAEMF  540
PFPMSSSTSL ASSSSKVNGC GSSLLNKTQV DAQMGSFMSL STTKSASASP ADNSDNTD

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
2ayd_A	7e-21	237	311	1	74	WRKY transcription factor 1
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription activator involved in the transcriptional regulation of terpene biosynthesis in glandular trichomes (PubMed:24884371). Binds to the promoter of the linalool synthase TPS5 and promotes TPS5 gene transactivation (PubMed:24884371). In association with WRKY72B, contributes to basal defense against root-knot nematodes (RKNs) and potato aphids, as well as Mi-1-mediated gene-for-gene resistance to these pests (PubMed:20409007). Both WRKY72A and WRKY72B are not required for gene-for-gene resistance mediated by Pto, another tomato R gene (PubMed:20409007). {ECO:0000269\|PubMed:20409007, ECO:0000269\|PubMed:24884371}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	XP_004508768.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Induced by infection with the root-knot nematode (RKN) Meloidogyne incognita and potato aphids. {ECO:0000269\|PubMed:20409007}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_004508768.1	0.0	probable WRKY transcription factor 72 isoform X2
Swissprot	A0A3Q7GFB5	1e-128	WK72A_SOLLC; WRKY transcription factor 72A
TrEMBL	A0A1S2YRH2	0.0	A0A1S2YRH2_CICAR; probable WRKY transcription factor 72 isoform X2
STRING	XP_004508768.1	0.0	(Cicer arietinum)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Fabids	OGEF650	34	134

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT5G15130.1	3e-64	WRKY DNA-binding protein 72

Publications ? help Back to Top
Tomato Genome Consortium The tomato genome sequence provides insights into fleshy fruit evolution. Nature, 2012. 485(7400): p. 635-41 [PMID:22660326] Spyropoulou EA,Haring MA,Schuurink RC RNA sequencing on Solanum lycopersicum trichomes identifies transcription factors that activate terpene synthase promoters. BMC Genomics, 2014. 15: p. 402 [PMID:24884371]