PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassicaceae incertae sedis; Schrenkiella

Family

WRKY

Protein Properties

Length: 513aa MW: 56355.9 Da PI: 8.1534

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Tp4g20800	genome	thellungiella	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	104	7.9e-33	183	240	2	60
--SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS-- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhek 60 +DgynWrKYGqK+vkgse+prsYY+Ct+++Cp+kkkvers +++++ei+Y+g+Hnh+k Tp4g20800 183 EDGYNWRKYGQKQVKGSENPRSYYKCTFPSCPTKKKVERSL-EGQITEIVYKGSHNHPK 240 7**************************************.*************85 PP
2	WRKY	105	3.9e-33	357	415	1	59
---SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 1 ldDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhe 59 ldDgy+WrKYGqK+vkg+++prsYY+Ct+ gCpv+k+ver+++d ++v++tYeg+Hnh+ Tp4g20800 357 LDDGYRWRKYGQKVVKGNPNPRSYYKCTTIGCPVRKHVERASHDMRAVITTYEGKHNHD 415 59********************************************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	2.1E-27	174	241	IPR003657	WRKY domain
SuperFamily	SSF118290	2.88E-24	182	241	IPR003657	WRKY domain
SMART	SM00774	1.0E-33	182	240	IPR003657	WRKY domain
PROSITE profile	PS50811	22.905	183	241	IPR003657	WRKY domain
Pfam	PF03106	7.7E-25	183	239	IPR003657	WRKY domain
Gene3D	G3DSA:2.20.25.80	6.3E-37	342	417	IPR003657	WRKY domain
SuperFamily	SSF118290	3.14E-29	349	417	IPR003657	WRKY domain
PROSITE profile	PS50811	38.434	352	417	IPR003657	WRKY domain
SMART	SM00774	1.8E-38	357	416	IPR003657	WRKY domain
Pfam	PF03106	2.0E-25	358	415	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0009409	Biological Process	response to cold
GO:0009414	Biological Process	response to water deprivation
GO:0009651	Biological Process	response to salt stress
GO:0009753	Biological Process	response to jasmonic acid
GO:0009788	Biological Process	negative regulation of abscisic acid-activated signaling pathway
GO:0009938	Biological Process	negative regulation of gibberellic acid mediated signaling pathway
GO:0010120	Biological Process	camalexin biosynthetic process
GO:0010200	Biological Process	response to chitin
GO:0010508	Biological Process	positive regulation of autophagy
GO:0042742	Biological Process	defense response to bacterium
GO:0045893	Biological Process	positive regulation of transcription, DNA-templated
GO:0050832	Biological Process	defense response to fungus
GO:0070370	Biological Process	cellular heat acclimation
GO:0005634	Cellular Component	nucleus
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding
GO:0044212	Molecular Function	transcription regulatory region DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 513 aa Download sequence Send to blast
MAASSLLTMD NTRTRQNMGG SANWSQQTGR SSASLEDLEI PKFRSFAPSS ISTSPSLVSP 60 STCFSPSLFL DSPAFVASSA NVLASPTTGA LITNGSSQKS INEEEKNKNN NNINFFDFSF 120 HTQSSGVSAP TTTTTTTANS SVFQSQEQQR KNQSDQWSQT ETRPSNNTHQ AVSYNGREQR 180 KGEDGYNWRK YGQKQVKGSE NPRSYYKCTF PSCPTKKKVE RSLEGQITEI VYKGSHNHPK 240 PQSTRRSSSS STFHSAVFNA GLDHHGSSDQ PNSNNSFHHS DSFAIQHEDN TTSGSVGDDE 300 FERGSSVISR DEEDCGSEPE AKRWKGENET NGGNGNGSKT VREPRIVVQT TSDIDILDDG 360 YRWRKYGQKV VKGNPNPRSY YKCTTIGCPV RKHVERASHD MRAVITTYEG KHNHDVPAAR 420 GSGYATNRPS QDPSSAPIRP AAIAGHTNYT TSSQAPYTLQ MLHNNTNAGP FGYAMNNNNS 480 NLQTQQSNFV GSGFSRAKEE PNEESSFFDS FLS

Sequence ? help Back to Top

Protein Sequence Length: 513 aa Download sequence Send to blast

MAASSLLTMD NTRTRQNMGG SANWSQQTGR SSASLEDLEI PKFRSFAPSS ISTSPSLVSP  60
STCFSPSLFL DSPAFVASSA NVLASPTTGA LITNGSSQKS INEEEKNKNN NNINFFDFSF  120
HTQSSGVSAP TTTTTTTANS SVFQSQEQQR KNQSDQWSQT ETRPSNNTHQ AVSYNGREQR  180
KGEDGYNWRK YGQKQVKGSE NPRSYYKCTF PSCPTKKKVE RSLEGQITEI VYKGSHNHPK  240
PQSTRRSSSS STFHSAVFNA GLDHHGSSDQ PNSNNSFHHS DSFAIQHEDN TTSGSVGDDE  300
FERGSSVISR DEEDCGSEPE AKRWKGENET NGGNGNGSKT VREPRIVVQT TSDIDILDDG  360
YRWRKYGQKV VKGNPNPRSY YKCTTIGCPV RKHVERASHD MRAVITTYEG KHNHDVPAAR  420
GSGYATNRPS QDPSSAPIRP AAIAGHTNYT TSSQAPYTLQ MLHNNTNAGP FGYAMNNNNS  480
NLQTQQSNFV GSGFSRAKEE PNEESSFFDS FLS

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1wj2_A	2e-37	183	418	8	78	Probable WRKY transcription factor 4
2lex_A	2e-37	183	418	8	78	Probable WRKY transcription factor 4
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Interacts specifically with the W box (5'-TTGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Involved in defense responses. Required for resistance to the necrotrophic fungal pathogen B.cinerea (PubMed:17059405, PubMed:21990940). Regulates the antagonistic relationship between defense pathways mediating responses to the bacterial pathogen P. syringae and the necrotrophic pathogen B.cinerea (PubMed:17059405). Required for the phytoalexin camalexin synthesis following infection with B.cinerea. Acts as positive regulator of the camalexin biosynthetic genes PAD3 (CYP71B15) and CYP71A13 by binding to their promoters (PubMed:21498677, PubMed:22392279). Acts downstream of MPK3 and MPK6 in reprogramming the expression of camalexin biosynthetic genes, which drives the metabolic flow to camalexin production (PubMed:21498677). Functions with WRKY25 as positive regulator of salt stress response and abscisic acid (ABA) signaling (PubMed:18839316). Functions with WRKY25 and WRKY26 as positive regulator of plant thermotolerance by partially participating in ethylene-response signal transduction pathway (PubMed:21336597). The DNA-binding activity of WRKY33 is increased by SIB1 and SIB2 (PubMed:21990940). {ECO:0000269\|PubMed:18839316, ECO:0000269\|PubMed:21336597, ECO:0000269\|PubMed:21498677, ECO:0000269\|PubMed:21990940, ECO:0000269\|PubMed:22392279}.

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00299	DAP	Transfer from AT2G38470	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Tp4g20800

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By salt stress (PubMed:18839316). Induced by infection with the necrotrophic fungal pathogen B.cinerea (PubMed:17059405, PubMed:21498677, PubMed:21990940). Induced by infection with the bacterial pathogen P.syringae pv. tomato DC3000 (PubMed:17059405). {ECO:0000269\|PubMed:17059405, ECO:0000269\|PubMed:18839316, ECO:0000269\|PubMed:21498677, ECO:0000269\|PubMed:21990940}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	-	Retrieve

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AK352683	0.0	AK352683.1 Thellungiella halophila mRNA, complete cds, clone: RTFL01-03-H12.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_006411061.1	0.0	probable WRKY transcription factor 33
Swissprot	Q8S8P5	0.0	WRK33_ARATH; Probable WRKY transcription factor 33
TrEMBL	E4MVY6	0.0	E4MVY6_EUTHA; mRNA, clone: RTFL01-03-H12
TrEMBL	V4M890	0.0	V4M890_EUTSA; Uncharacterized protein
STRING	XP_006411061.1	0.0	(Eutrema salsugineum)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM4583	27	55

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT2G38470.1	0.0	WRKY DNA-binding protein 33

Publications ? help Back to Top
Brand LH,Kirchler T,Hummel S,Chaban C,Wanke D DPI-ELISA: a fast and versatile method to specify the binding of plant transcription factors to DNA in vitro. Plant Methods, 2010. 6: p. 25 [PMID:21108821] Brand LH, et al. Screening for protein-DNA interactions by automatable DNA-protein interaction ELISA. PLoS ONE, 2013. 8(10): p. e75177 [PMID:24146751] Ali MA,Wieczorek K,Kreil DP,Bohlmann H The beet cyst nematode Heterodera schachtii modulates the expression of WRKY transcription factors in syncytia to favour its development in Arabidopsis roots. PLoS ONE, 2014. 9(7): p. e102360 [PMID:25033038] Divi UK,Rahman T,Krishna P Gene expression and functional analyses in brassinosteroid-mediated stress tolerance. Plant Biotechnol. J., 2016. 14(1): p. 419-32 [PMID:25973891] Peskan-Berghöfer T, et al. Sustained exposure to abscisic acid enhances the colonization potential of the mutualist fungus Piriformospora indica on Arabidopsis thaliana roots. New Phytol., 2015. 208(3): p. 873-86 [PMID:26075497] Wang C, et al. The Arabidopsis Mediator Complex Subunit16 Is a Key Component of Basal Resistance against the Necrotrophic Fungal Pathogen Sclerotinia sclerotiorum. Plant Physiol., 2015. 169(1): p. 856-72 [PMID:26143252] Wang C, et al. Arabidopsis Elongator subunit 2 positively contributes to resistance to the necrotrophic fungal pathogens Botrytis cinerea and Alternaria brassicicola. Plant J., 2015. 83(6): p. 1019-33 [PMID:26216741] Datta R, et al. Glutathione Regulates 1-Aminocyclopropane-1-Carboxylate Synthase Transcription via WRKY33 and 1-Aminocyclopropane-1-Carboxylate Oxidase by Modulating Messenger RNA Stability to Induce Ethylene Synthesis during Stress. Plant Physiol., 2015. 169(4): p. 2963-81 [PMID:26463088] Daumann M,Fischer M,Niopek-Witz S,Girke C,Möhlmann T Apoplastic Nucleoside Accumulation in Arabidopsis Leads to Reduced Photosynthetic Performance and Increased Susceptibility Against Botrytis cinerea. Front Plant Sci, 2015. 6: p. 1158 [PMID:26779190] Liu S,Bartnikas LM,Volko SM,Ausubel FM,Tang D Mutation of the Glucosinolate Biosynthesis Enzyme Cytochrome P450 83A1 Monooxygenase Increases Camalexin Accumulation and Powdery Mildew Resistance. Front Plant Sci, 2016. 7: p. 227 [PMID:26973671] Jiang Y,Yu D The WRKY57 Transcription Factor Affects the Expression of Jasmonate ZIM-Domain Genes Transcriptionally to Compromise Botrytis cinerea Resistance. Plant Physiol., 2016. 171(4): p. 2771-82 [PMID:27268959] Liao CJ,Lai Z,Lee S,Yun DJ,Mengiste T Arabidopsis HOOKLESS1 Regulates Responses to Pathogens and Abscisic Acid through Interaction with MED18 and Acetylation of WRKY33 and ABI5 Chromatin. Plant Cell, 2016. 28(7): p. 1662-81 [PMID:27317674] Birkenbihl RP,Kracher B,Roccaro M,Somssich IE Induced Genome-Wide Binding of Three Arabidopsis WRKY Transcription Factors during Early MAMP-Triggered Immunity. Plant Cell, 2017. 29(1): p. 20-38 [PMID:28011690] Nguyen CC, et al. Overexpression of oligouridylate binding protein 1b results in ABA hypersensitivity. Plant Signal Behav, 2017. 12(2): p. e1282591 [PMID:28112571] Liu S,Ziegler J,Zeier J,Birkenbihl RP,Somssich IE Botrytis cinerea B05.10 promotes disease development in Arabidopsis by suppressing WRKY33-mediated host immunity. Plant Cell Environ., 2017. 40(10): p. 2189-2206 [PMID:28708934] D'Ambrosio JM, et al. Phospholipase C2 Affects MAMP-Triggered Immunity by Modulating ROS Production. Plant Physiol., 2017. 175(2): p. 970-981 [PMID:28827453] Liu F, et al. Interactions of WRKY15 and WRKY33 transcription factors and their roles in the resistance of oilseed rape to Sclerotinia infection. Plant Biotechnol. J., 2018. 16(4): p. 911-925 [PMID:28929638] Crespo-Salvador Ó,Escamilla-Aguilar M,López-Cruz J,López-Rodas G,González-Bosch C Determination of histone epigenetic marks in Arabidopsis and tomato genes in the early response to Botrytis cinerea. Plant Cell Rep., 2018. 37(1): p. 153-166 [PMID:29119291]