PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Rsa1.0_00311.1_g00013.1

Organism

Raphanus sativus

Taxonomic ID

3726

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Raphanus

Family

WRKY

Protein Properties

Length: 576aa MW: 64139.3 Da PI: 8.8995

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Rsa1.0_00311.1_g00013.1	genome	RGD	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	25.5	2.7e-08	181	202	2	23
--SS-EEEEEEE--TT-SS-EE CS WRKY 2 dDgynWrKYGqKevkgsefprs 23 +Dgy+WrKYGqK vk+++ s Rsa1.0_00311.1_g00013.1 181 EDGYRWRKYGQKAVKNKTKRSS 202 8**************9866544 PP
2	WRKY	53.5	4.7e-17	456	492	23	59
EEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 23 sYYrCtsagCpvkkkversaedpkvveitYegeHnhe 59 sYYrCt+++C+vkk+vers +dp+vv++tYe++Hnh+ Rsa1.0_00311.1_g00013.1 456 SYYRCTTQKCNVKKRVERSYQDPTVVITTYESQHNHP 492 9***********************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	9.8E-10	165	204	IPR003657	WRKY domain
SuperFamily	SSF118290	5.62E-7	173	204	IPR003657	WRKY domain
PROSITE profile	PS50811	10.085	175	204	IPR003657	WRKY domain
SMART	SM00774	0.0074	180	224	IPR003657	WRKY domain
Pfam	PF03106	3.2E-5	181	204	IPR003657	WRKY domain
SMART	SM00774	3.7E-9	445	493	IPR003657	WRKY domain
SuperFamily	SSF118290	2.35E-13	455	494	IPR003657	WRKY domain
Pfam	PF03106	3.6E-11	455	492	IPR003657	WRKY domain
Gene3D	G3DSA:2.20.25.80	1.0E-15	456	494	IPR003657	WRKY domain
PROSITE profile	PS50811	17.959	456	494	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 576 aa Download sequence Send to blast
MSNETKDLNN HHHTSSYNHY NDISNQNILN LPYVSGPSSY NANMISSQIG YDLQSSPQGV 60 YESGFELSPS FTEFFSSSFD QENGFYNAFN YNTSNKSQEV VGSGGAIIES KTRGSASPSS 120 SEADHHHGED SGNSLRKREA DDGGKDDQRA QKEVKTKRKE EKKQREPRVS FVTKTEVEHL 180 EDGYRWRKYG QKAVKNKTKR SSKYHQHILS LLSLHVVAAV NHHRLLFISF LTFFTFLFCL 240 TLFSSSLHSA ATTSHSLSSS PPSSSLSPPI LAALLHYTSS SPPNTSMSLP ELSAVSTVIN 300 SLRDAQLRPQ TPLPHRDLSV LPPLLRLPYP PHEPPINLCD SFSSAAVSIA ASAPSGTPSS 360 APGLRSHTEI SLSQPLAPSS STYASSATPS LPPPLHSLLL HHIRRHHRLL PRHLPSLLLL 420 RSQPRRLPLS PPLPFGDDGG DVAHTLLHNG LARDWSYYRC TTQKCNVKKR VERSYQDPTV 480 VITTYESQHN HPIPTSRRSA MFAGPASSNY NSTSLSSVSD FIINTPRSFS RDDLFRAPYA 540 SVNENPNYQQ QQNQELQHDY ELLKDMFPSV FFKHEP

Sequence ? help Back to Top

Protein Sequence Length: 576 aa Download sequence Send to blast

MSNETKDLNN HHHTSSYNHY NDISNQNILN LPYVSGPSSY NANMISSQIG YDLQSSPQGV  60
YESGFELSPS FTEFFSSSFD QENGFYNAFN YNTSNKSQEV VGSGGAIIES KTRGSASPSS  120
SEADHHHGED SGNSLRKREA DDGGKDDQRA QKEVKTKRKE EKKQREPRVS FVTKTEVEHL  180
EDGYRWRKYG QKAVKNKTKR SSKYHQHILS LLSLHVVAAV NHHRLLFISF LTFFTFLFCL  240
TLFSSSLHSA ATTSHSLSSS PPSSSLSPPI LAALLHYTSS SPPNTSMSLP ELSAVSTVIN  300
SLRDAQLRPQ TPLPHRDLSV LPPLLRLPYP PHEPPINLCD SFSSAAVSIA ASAPSGTPSS  360
APGLRSHTEI SLSQPLAPSS STYASSATPS LPPPLHSLLL HHIRRHHRLL PRHLPSLLLL  420
RSQPRRLPLS PPLPFGDDGG DVAHTLLHNG LARDWSYYRC TTQKCNVKKR VERSYQDPTV  480
VITTYESQHN HPIPTSRRSA MFAGPASSNY NSTSLSSVSD FIINTPRSFS RDDLFRAPYA  540
SVNENPNYQQ QQNQELQHDY ELLKDMFPSV FFKHEP

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Interacts specifically with the W box (5'-TTGAC[CT]-3'), a frequently occurring stress-responsive cis-acting element. Functions as positive regulator of salt stress response. Binds the W box of LTI78/RD29A stress-response gene and directly regulates its transcription under salt stress. Functions antagonistically with VQ9 to regulate sodium and potassium homeostasis under salt stress by regulating the expression of downstream SOS (SALT OVERLY SENSITIVE) stress-responsive genes. The DNA-binding activity of WRKY8 is decreased by VQ9 (PubMed:23451802). Functions as negative regulator of basal resistance to the bacterial pathogen P. syringae and as positive regulator of resistance to the fungal pathogen to B. cinerea (PubMed:20367464). Functions as positive regulator of defense response againt tobamovirus (TMV) by regulating both the abscisic acid and ethylene signaling pathways. Positively regulates ABI4 expression and negatively modulates ACS6 and ERF104 expression by directly binding to the W box consensus motifs within their promoters (PubMed:23650359). {ECO:0000269\|PubMed:20367464, ECO:0000269\|PubMed:23451802, ECO:0000269\|PubMed:23650359}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Rsa1.0_00311.1_g00013.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By wounding, abscisic acid (ABA), salicylic acid (SA), H(2)O(2), and infection with P.syringae pv. tomato DC3000 and B.cinerea (PubMed:20367464). Induced by salt stress (PubMed:23451802). {ECO:0000269\|PubMed:20367464, ECO:0000269\|PubMed:23451802}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	KC246569	0.0	KC246569.1 Brassica napus WRKY transcription factor 8.1 (WRKY8.1) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_018481874.1	1e-122	PREDICTED: probable WRKY transcription factor 8
Swissprot	Q9FL26	1e-83	WRKY8_ARATH; WRKY transcription factor 8

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM1524	26	93

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT5G46350.1	4e-64	WRKY DNA-binding protein 8

Publications ? help Back to Top
Duarte JM, et al. Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. Mol. Biol. Evol., 2006. 23(2): p. 469-78 [PMID:16280546] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Sukweenadhi J, et al. Paenibacillus yonginensis DCY84(T) induces changes in Arabidopsis thaliana gene expression against aluminum, drought, and salt stress. Microbiol. Res., 2015. 172: p. 7-15 [PMID:25721473]