PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Rsa1.0_00090.1_g00028.1

Organism

Raphanus sativus

Taxonomic ID

3726

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Raphanus

Family

WRKY

Protein Properties

Length: 1016aa MW: 114506 Da PI: 8.1065

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Rsa1.0_00090.1_g00028.1	genome	RGD	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	99.7	1.8e-31	256	311	2	58
--SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnh 58 +DgynWrKYGqK vkgse+prsYY+Ct ++Cpvkkkvers+ +++++ei+Y+g Hnh Rsa1.0_00090.1_g00028.1 256 EDGYNWRKYGQKLVKGSEYPRSYYKCTNPSCPVKKKVERSR-EGHITEIIYKGAHNH 311 8**************************************.************* PP
2	WRKY	101.7	4.3e-32	456	514	1	59
---SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 1 ldDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhe 59 ldDgy+WrKYGqK+vkg+++prsYY+Ct +gC+v+k+ver+++d k v++tYeg+H+h+ Rsa1.0_00090.1_g00028.1 456 LDDGYRWRKYGQKVVKGNPNPRSYYKCTAPGCTVRKHVERASHDLKSVITTYEGKHDHD 514 59********************************************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS50811	23.571	250	314	IPR003657	WRKY domain
Gene3D	G3DSA:2.20.25.80	9.5E-28	250	314	IPR003657	WRKY domain
SuperFamily	SSF118290	2.22E-24	253	314	IPR003657	WRKY domain
SMART	SM00774	1.5E-33	255	313	IPR003657	WRKY domain
Pfam	PF03106	5.6E-24	256	311	IPR003657	WRKY domain
Gene3D	G3DSA:2.20.25.80	6.6E-36	441	516	IPR003657	WRKY domain
SuperFamily	SSF118290	6.93E-28	448	516	IPR003657	WRKY domain
PROSITE profile	PS50811	37.677	451	516	IPR003657	WRKY domain
SMART	SM00774	1.6E-36	456	515	IPR003657	WRKY domain
Pfam	PF03106	5.7E-24	457	513	IPR003657	WRKY domain
Pfam	PF13966	5.5E-18	833	917	IPR026960	Reverse transcriptase zinc-binding domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 1016 aa Download sequence Send to blast
MSGFEENVAV MGEWVPCNPT SRRVLERELS LNHGLVNRLE EEEEDTTGNN KNFSRGGGLR 60 ERIAARAGFN TPRLNTENIR SNTDCSLRSP NCLTISSPGL SPATLLESPV FLSNPLAQPS 120 PTTGKFPFLP GVNSSNGLSS SSDKAKEEFF DGIGASSFTF HPVSRSSSSF FHGAVEMMPV 180 GYGNYNNRSS HLSPEEDVKL TSQNIESSRT ETEQSGLKRK TSDATTNTVD HQEEEEEEEG 240 EEQRGGDSMA AGAPAEDGYN WRKYGQKLVK GSEYPRSYYK CTNPSCPVKK KVERSREGHI 300 TEIIYKGAHN HFKPPPNRRS GTQLDGTEQQ QQQRDNSATW VSCTNTQQQA GSNENNVEEE 360 DGNHSGSIQA QESGGVIVVD ALSTLSNDED EDDRGTHGSA SLGYDRCGGG GGEGDESESE 420 SKRRKLEAYA IEMSGATRAI REPRVVVQTT SDVDILDDGY RWRKYGQKVV KGNPNPRSYY 480 KCTAPGCTVR KHVERASHDL KSVITTYEGK HDHDVPAARN SSHGGGNGNS APQTNHYQDP 540 PRGRYDRQLN QSSQFGRPFS FQPHLGPPSG FAFGVGQTGF GNLSIPGLAF GQGKLQGLSH 600 PSYMSQQPVG MSEGMMMMQR GMEPKVEPGS ETGQSKDEQP LHTNEYSAKI QKERMIAGIN 660 TFWCSAFVLP KAFIKRINSL SSIFLWKVSI EGHNTARVSW ETVVLTKRQG GLGIKDLLTW 720 NKACTLRLIW LLFFRPESVW VQWFKKVILK GSLHNYWTTK PRQSYSWLVN KLLKLKQTVY 780 PLLKLTLRNG KSARFWTDNW SPFGELNTYL SGAVSRWQDY FTWEVNGQEY DSFKTGALYD 840 YLREPHADVD WADAVWFTRA IPRHAFHTWL VIQDRIPTRD RLLRWGLGVD GACLLCNSAQ 900 ESRDHIYFNC DYGYNLWRKI TERLRLVPHR NWADTVEQMN SLPPPTAQRS LTLLAWQSTL 960 YWVWNERNAR LHSNTFRSID QLFKVIDTQL RNKLQSFRGD NPTRLSHMMQ NWFRSA

Sequence ? help Back to Top

Protein Sequence Length: 1016 aa Download sequence Send to blast

MSGFEENVAV MGEWVPCNPT SRRVLERELS LNHGLVNRLE EEEEDTTGNN KNFSRGGGLR  60
ERIAARAGFN TPRLNTENIR SNTDCSLRSP NCLTISSPGL SPATLLESPV FLSNPLAQPS  120
PTTGKFPFLP GVNSSNGLSS SSDKAKEEFF DGIGASSFTF HPVSRSSSSF FHGAVEMMPV  180
GYGNYNNRSS HLSPEEDVKL TSQNIESSRT ETEQSGLKRK TSDATTNTVD HQEEEEEEEG  240
EEQRGGDSMA AGAPAEDGYN WRKYGQKLVK GSEYPRSYYK CTNPSCPVKK KVERSREGHI  300
TEIIYKGAHN HFKPPPNRRS GTQLDGTEQQ QQQRDNSATW VSCTNTQQQA GSNENNVEEE  360
DGNHSGSIQA QESGGVIVVD ALSTLSNDED EDDRGTHGSA SLGYDRCGGG GGEGDESESE  420
SKRRKLEAYA IEMSGATRAI REPRVVVQTT SDVDILDDGY RWRKYGQKVV KGNPNPRSYY  480
KCTAPGCTVR KHVERASHDL KSVITTYEGK HDHDVPAARN SSHGGGNGNS APQTNHYQDP  540
PRGRYDRQLN QSSQFGRPFS FQPHLGPPSG FAFGVGQTGF GNLSIPGLAF GQGKLQGLSH  600
PSYMSQQPVG MSEGMMMMQR GMEPKVEPGS ETGQSKDEQP LHTNEYSAKI QKERMIAGIN  660
TFWCSAFVLP KAFIKRINSL SSIFLWKVSI EGHNTARVSW ETVVLTKRQG GLGIKDLLTW  720
NKACTLRLIW LLFFRPESVW VQWFKKVILK GSLHNYWTTK PRQSYSWLVN KLLKLKQTVY  780
PLLKLTLRNG KSARFWTDNW SPFGELNTYL SGAVSRWQDY FTWEVNGQEY DSFKTGALYD  840
YLREPHADVD WADAVWFTRA IPRHAFHTWL VIQDRIPTRD RLLRWGLGVD GACLLCNSAQ  900
ESRDHIYFNC DYGYNLWRKI TERLRLVPHR NWADTVEQMN SLPPPTAQRS LTLLAWQSTL  960
YWVWNERNAR LHSNTFRSID QLFKVIDTQL RNKLQSFRGD NPTRLSHMMQ NWFRSA

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1wj2_A	1e-35	256	517	8	78	Probable WRKY transcription factor 4
2lex_A	1e-35	256	517	8	78	Probable WRKY transcription factor 4
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Regulates WOX8 and WOX9 expression and basal cell division patterns during early embryogenesis. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Required to repolarize the zygote from a transient symmetric state. {ECO:0000269\|PubMed:21316593}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Rsa1.0_00090.1_g00028.1

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	FJ012166	1e-179	FJ012166.1 Brassica napus WRKY2 transcription factor (WRKY2) mRNA, partial cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_018472516.1	0.0	PREDICTED: probable WRKY transcription factor 2
Swissprot	Q9FG77	0.0	WRKY2_ARATH; Probable WRKY transcription factor 2
TrEMBL	V4LSF0	0.0	V4LSF0_EUTSA; Uncharacterized protein
STRING	XP_006401355.1	0.0	(Eutrema salsugineum)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM7954	27	40

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT5G56270.1	0.0	WRKY DNA-binding protein 2

Publications ? help Back to Top
Duarte JM, et al. Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. Mol. Biol. Evol., 2006. 23(2): p. 469-78 [PMID:16280546] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Ueda M, et al. Transcriptional integration of paternal and maternal factors in the Arabidopsis zygote. Genes Dev., 2017. 31(6): p. 617-627 [PMID:28404632] Lei R, et al. Arabidopsis WRKY2 and WRKY34 transcription factors interact with VQ20 protein to modulate pollen development and function. Plant J., 2017. 91(6): p. 962-976 [PMID:28635025]