PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

RrC2184_p4

Organism

Raphanus raphanistrum

Taxonomic ID

109996

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Raphanus

Family

WRKY

Protein Properties

Length: 305aa MW: 34159.1 Da PI: 5.321

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
RrC2184_p4	genome	MSU	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	88.5	5.8e-28	107	167	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-ST...T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsa...gCpvkkkversaedpkvveitYegeHnhe 59 dDgy+WrKYGqKe++gs +pr+Y+rCt++ +C + k+v++s++dp+++e++Y g+H+++ RrC2184_p4 107 DDGYCWRKYGQKEIHGSINPRGYFRCTHRftqNCLAVKQVQKSDTDPSIFEVKYVGNHTCN 167 8*************************9999*************************97 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	1.9E-25	101	168	IPR003657	WRKY domain
PROSITE profile	PS50811	21.471	101	169	IPR003657	WRKY domain
SuperFamily	SSF118290	1.2E-23	104	167	IPR003657	WRKY domain
SMART	SM00774	3.1E-35	106	168	IPR003657	WRKY domain
Pfam	PF03106	6.8E-23	107	166	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0010200	Biological Process	response to chitin
GO:0048527	Biological Process	lateral root development
GO:0005730	Cellular Component	nucleolus
GO:0000987	Molecular Function	core promoter proximal region sequence-specific DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 305 aa Download sequence Send to blast
MVMEGKLVIN ELEQGRELAK RLMSNLKDTS SAESNRNLIS EILSIYQNAI SRLSATEEDD 60 KNILKRSREI EDKDSKNVFK KRKISEKKTE KVSFFVGGAG QEKGSIDDGY CWRKYGQKEI 120 HGSINPRGYF RCTHRFTQNC LAVKQVQKSD TDPSIFEVKY VGNHTCNNTT TSSPKTPITT 180 NFSVSMCEGG NRVDIVTEKQ EDIIKPTKTE EATMVMSLAD LESKKNIFRT FSFCNYEFEN 240 AGGGGWKGNI FEENQLSPAT TSGSGITSEI VTGPAASVEN SETADSYFSS LDNIIDFGQD 300 WLWSF

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
6ir8_A	2e-21	105	166	7	68	OsWRKY45
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor involved in the regulation of osmotic stress responses and stomatal movement (PubMed:24773321). Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). Positive regulator of EDS1-dependent defense against E.amylovora (PubMed:22316300). Together with WRKY70 and WRKY53, promotes resistance to P.syringae, probably by enhancing salicylic acid (SA)- dependent genes. Contributes to the suppression of jasmonic acid (MeJA)-induced expression of PDF1.2 (PubMed:22325892). Together with WRKY54 and WRKY70, promotes brassinosteroid (BR)-regulated plant growth but prevent drought response by modulating gene expression (PubMed:28576847). {ECO:0000250, ECO:0000269\|PubMed:22316300, ECO:0000269\|PubMed:22325892, ECO:0000269\|PubMed:24773321, ECO:0000269\|PubMed:28576847}.

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00112	ampDAP	Transfer from AT2G46400	Download

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Up-regulated by drought, salt and hydrogen peroxide treatments (PubMed:24773321). Induced by salicylic acid (SA) (PubMed:22268143, PubMed:22325892). Up-regulated by E.amylovora (PubMed:22316300). Triggered by P.syringae (PubMed:22325892). {ECO:0000269\|PubMed:22268143, ECO:0000269\|PubMed:22316300, ECO:0000269\|PubMed:22325892, ECO:0000269\|PubMed:24773321}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_018441250.1	0.0	PREDICTED: probable WRKY transcription factor 46
Refseq	XP_018441366.1	0.0	PREDICTED: probable WRKY transcription factor 46
Swissprot	Q9SKD9	1e-144	WRK46_ARATH; Probable WRKY transcription factor 46
TrEMBL	A0A397Z613	1e-172	A0A397Z613_BRACM; Uncharacterized protein
STRING	Bo4g009270.1	1e-164	(Brassica oleracea)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM4888	23	52

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT2G46400.1	1e-127	WRKY DNA-binding protein 46

Publications ? help Back to Top
Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Sheikh AH, et al. Regulation of WRKY46 Transcription Factor Function by Mitogen-Activated Protein Kinases in Arabidopsis thaliana. Front Plant Sci, 2016. 7: p. 61 [PMID:26870073] Yan JY, et al. A WRKY Transcription Factor Regulates Fe Translocation under Fe Deficiency. Plant Physiol., 2016. 171(3): p. 2017-27 [PMID:27208259] Chen J, et al. Arabidopsis WRKY46, WRKY54, and WRKY70 Transcription Factors Are Involved in Brassinosteroid-Regulated Plant Growth and Drought Responses. Plant Cell, 2017. 29(6): p. 1425-1439 [PMID:28576847] Ma J, et al. Overexpression of TaWRKY146 Increases Drought Tolerance through Inducing Stomatal Closure in Arabidopsis thaliana. Front Plant Sci, 2017. 8: p. 2036 [PMID:29225611]