PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Malpighiales; Salicaceae; Saliceae; Populus

Family

ERF

Protein Properties

Length: 97aa MW: 11012.4 Da PI: 10.8503

Description

ERF family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Potri.014G076700.1	genome	JGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	AP2	66.9	3.9e-21	39	89	2	56
AP2 2 gykGVrwdkkrgrWvAeIrdpsengkrkrfslgkfgtaeeAakaaiaarkklege 56 ++ GVr+++ +grWvAeI+d s +r r +lg+++t+eeAa+a+++a+++l+ge Potri.014G076700.1 39 RFVGVRQRP-SGRWVAEIKDSS---QRVRLWLGTYDTPEEAARAYDEAARALRGE 89 689****.7*****43...36************************97 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
CDD	cd00018	7.17E-29	39	96	No hit	No description
PROSITE profile	PS51032	20.534	39	96	IPR001471	AP2/ERF domain
SMART	SM00380	1.6E-31	39	97	IPR001471	AP2/ERF domain
Gene3D	G3DSA:3.30.730.10	3.1E-29	39	96	IPR001471	AP2/ERF domain
Pfam	PF00847	3.9E-16	39	89	IPR001471	AP2/ERF domain
SuperFamily	SSF54171	6.28E-21	40	96	IPR016177	DNA-binding domain
PRINTS	PR00367	6.0E-8	40	51	IPR001471	AP2/ERF domain
PRINTS	PR00367	6.0E-8	62	78	IPR001471	AP2/ERF domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0005634	Cellular Component	nucleus
GO:0003677	Molecular Function	DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 97 aa Download sequence Send to blast
MRGIKTVEKT KYEEPETLRN SPTVTTTKVQ ATKGPTLRRF VGVRQRPSGR WVAEIKDSSQ 60 RVRLWLGTYD TPEEAARAYD EAARALRGEN ARTNFAS

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
5wx9_A	2e-15	34	95	9	71	Ethylene-responsive transcription factor ERF096
Search in ModeBase

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Expressed in roots, root hairs and leaves (PubMed:17827349). Expressed in root epidermis and root hairs (PubMed:23077241). {ECO:0000269\|PubMed:17827349, ECO:0000269\|PubMed:23077241}.

Functional Description ? help Back to Top
Source	Description
UniProt	Probably acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways (By similarity). {ECO:0000250}.
UniProt	Transcription factor involved in symbiotic nodule signaling in response to rhizobial Nod factors (NFs) (PubMed:17449807, PubMed:17827349). Binds to the GCC-box (NF-responsive box) of ENOD11 promoter. Acts as transcriptional activator of NF-responsive box-containing target gene promoters in root hairs (PubMed:17827349). Functions as a transcriptional regulator required for root infection by symbiotic rhizobia, infection thread (IT) formation and maintenance, and nodule development. Necessary for NF-induced gene expression and spontaneous nodulation activated by CCAMK. Functions downstream of CCAMK to activate nodulation gene expression (PubMed:17449807). Involved in early stages of root nodule development. Functions redundantly with ERN2. Is essential with ERN2 for the initiation of root hair infection, and nodule organogenesis and development. Required for accurate expression of the NF signaling genes ENOD11 and ENOD12 (PubMed:23077241, PubMed:27208242). {ECO:0000269\|PubMed:17449807, ECO:0000269\|PubMed:17827349, ECO:0000269\|PubMed:23077241, ECO:0000269\|PubMed:27208242}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Potri.014G076700.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Induced in roots from 1 to 21 day after inoculation with Sinorhizobium meliloti (PubMed:17449807). Induced by Nod factors in root hairs (PubMed:17827349). {ECO:0000269\|PubMed:17449807, ECO:0000269\|PubMed:17827349}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AC182673	8e-75	AC182673.2 Populus trichocarpa clone Pop1-26A1, complete sequence.
GenBank	AC183426	8e-75	AC183426.2 Populus trichocarpa clone Pop1-45L17, complete sequence.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_002320765.1	3e-50	uncharacterized protein LOC7464901
Swissprot	A2Q5W1	4e-27	ERN1_MEDTR; Ethylene-responsive transcription factor ERN1
Swissprot	Q6J9S1	3e-27	RA211_ARATH; Ethylene-responsive transcription factor RAP2-11
TrEMBL	A0A3N7HQ20	2e-54	A0A3N7HQ20_POPTR; Uncharacterized protein
STRING	POPTR_0014s07280.1	1e-49	(Populus trichocarpa)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Fabids	OGEF8324	31	43
Representative plant	OGRP6	16	1718

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT5G19790.1	3e-18	related to AP2 11

Link Out ? help Back to Top
Phytozome	Potri.014G076700.1

Publications ? help Back to Top
Cerri MR, et al. Medicago truncatula ERN transcription factors: regulatory interplay with NSP1/NSP2 GRAS factors and expression dynamics throughout rhizobial infection. Plant Physiol., 2012. 160(4): p. 2155-72 [PMID:23077241] Tang H, et al. An improved genome release (version Mt4.0) for the model legume Medicago truncatula. BMC Genomics, 2014. 15: p. 312 [PMID:24767513] Meng S, et al. Arabidopsis NRT1.5 Mediates the Suppression of Nitrate Starvation-Induced Leaf Senescence by Modulating Foliar Potassium Level. Mol Plant, 2016. 9(3): p. 461-470 [PMID:26732494] Cerri MR, et al. The Symbiosis-Related ERN Transcription Factors Act in Concert to Coordinate Rhizobial Host Root Infection. Plant Physiol., 2016. 171(2): p. 1037-54 [PMID:27208242]