PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Malpighiales; Salicaceae; Saliceae; Populus

Family

MYB

Protein Properties

Length: 304aa MW: 34361 Da PI: 5.1295

Description

MYB family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Potri.014G054700.1	genome	JGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	Myb_DNA-binding	44.7	3.2e-14	11	56	1	46
TSSS-HHHHHHHHHHHHHTTTT-HHHHHHHHTTTS-HHHHHHHHHH CS Myb_DNA-binding 1 rgrWTteEdellvdavkqlGggtWktIartmgkgRtlkqcksrwqk 46 +g+W +eEde l++ vk++G+++W++I ++ ++Rt+k+c++rw + Potri.014G054700.1 11 KGPWKEEEDEVLINHVKKYGPRDWSSIRSKGLLHRTGKSCRLRWVN 56 79***********************888779*********87 PP
2	Myb_DNA-binding	40.9	4.8e-13	67	106	3	44
SS-HHHHHHHHHHHHHTTTT-HHHHHHHHTTTS-HHHHHHHH CS Myb_DNA-binding 3 rWTteEdellvdavkqlGggtWktIartmgkgRtlkqcksrw 44 ++++eE+ +d+ ++ G++ W++Ia +++ gRt++++k++w Potri.014G054700.1 67 KFSAEEERVVIDLQAEIGNR-WARIATYLP-GRTDNDVKNFW 106 79****************.*****.********* PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:1.10.10.60	9.8E-22	5	59	IPR009057	Homeodomain-like
PROSITE profile	PS51294	23.208	6	62	IPR017930	Myb domain
SuperFamily	SSF46689	3.01E-28	9	106	IPR009057	Homeodomain-like
SMART	SM00717	1.3E-12	10	60	IPR001005	SANT/Myb domain
CDD	cd00167	5.47E-13	13	58	No hit	No description
Pfam	PF13921	2.5E-12	14	64	No hit	No description
Gene3D	G3DSA:1.10.10.60	1.4E-17	60	112	IPR009057	Homeodomain-like
PROSITE profile	PS51294	17.375	63	114	IPR017930	Myb domain
SMART	SM00717	2.2E-11	64	112	IPR001005	SANT/Myb domain
CDD	cd00167	1.25E-9	67	106	No hit	No description
Pfam	PF00249	2.5E-11	67	106	IPR001005	SANT/Myb domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0003677	Molecular Function	DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 304 aa Download sequence Send to blast
MEGFKKEEIR KGPWKEEEDE VLINHVKKYG PRDWSSIRSK GLLHRTGKSC RLRWVNKLRP 60 NLKNGCKFSA EEERVVIDLQ AEIGNRWARI ATYLPGRTDN DVKNFWSSRQ KRLARILQTS 120 GTPPSSFNSK QQRSKNKVPV FLDVPTFEAP MFSSSMEEEV SSKACSSSYL ENPEQIRMMP 180 LPPLVKSELP GCDANLVQYE PMSGIPFPQI PQPQPDLVFS PESHELLARL DDPYFLNVLG 240 TIDAPELGDT AQFSLGPPLF DPVSSCMNSP SDVKNPVTPD TLFDELPPDM FDHIEPFPSP 300 SEW*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1h8a_C	5e-29	8	112	24	126	MYB TRANSFORMING PROTEIN
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Expression -- Description ? help Back to Top
Source	Description
Uniprot	DEVELOPMENTAL STAGE: Strict spatial and temporal transcriptional activation leading to a specific expression in the male germline cells; this specific expression pattern is dependent of the regulatory region of DUO1 (ROD1) 5'-YAACYGY-3' in its promoter (PubMed:15694308, PubMed:27624837). First observed in the germ cell during or soon after engulfment by the vegetative cell, just after asymmetric division. In maturating pollen, accumulates in germ cells before mitosis and remains high in mature sperm cells. Expression persists during pollen development (PubMed:19300502). {ECO:0000269\|PubMed:15694308, ECO:0000269\|PubMed:19300502, ECO:0000269\|PubMed:27624837}.
Uniprot	TISSUE SPECIFICITY: Confined to inflorescences, especially in stamens and pollen. {ECO:0000269\|PubMed:15694308}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription activator that acts as a positive regulator of male germline development by promoting both gametic cell specification and cell cycle progression (PubMed:15694308, PubMed:15618418, PubMed:19300502, PubMed:21285328). Binds to canonical MYB sites 5'-AACCGTC-3', 5'-AAACCGC-3' and 5'-AACCGT-3' in promoters to trigger the expression of male germline-specific or enriched genes (e.g. MGH3, GEX2 and GCS1), including those required for fertilization (PubMed:21285328, PubMed:19300502). Required for sperm cell specification leading to pollen maturation by activating a germline-specific regulon (PubMed:21285328, PubMed:15694308, PubMed:15618418, PubMed:19300502). Involved in pollen mitosis entry at G2-M transition via the regulation of CYCB1-1, DAZ1 and DAZ2 expression (PubMed:15618418, PubMed:19300502, PubMed:24876252). {ECO:0000269\|PubMed:15618418, ECO:0000269\|PubMed:15694308, ECO:0000269\|PubMed:19300502, ECO:0000269\|PubMed:21285328, ECO:0000269\|PubMed:24876252}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Potri.014G054700.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Repressed by the microRNA 159 (miR159); the production of miR159 is stimulated by the anaphase promoting complex/cyclosome (APC/C) (PubMed:21441434). Activated by ARID1 in male germline cells via specific histone acetylation regulation (e.g. H3K9Ac) (PubMed:25057814). {ECO:0000269\|PubMed:21441434, ECO:0000269\|PubMed:25057814}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_006375223.1	0.0	transcription factor DUO1
Swissprot	A0A178VEK7	2e-91	DUO1_ARATH; Transcription factor DUO1
TrEMBL	U5FPT6	0.0	U5FPT6_POPTR; Uncharacterized protein
STRING	POPTR_0014s05370.1	0.0	(Populus trichocarpa)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Fabids	OGEF3693	31	64
Representative plant	OGRP5	17	1784

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT3G60460.1	2e-89	MYB family protein

Link Out ? help Back to Top
Phytozome	Potri.014G054700.1
Entrez Gene	18105133

Publications ? help Back to Top
Zheng B,He H,Zheng Y,Wu W,McCormick S An ARID domain-containing protein within nuclear bodies is required for sperm cell formation in Arabidopsis thaliana. PLoS Genet., 2014. 10(7): p. e1004421 [PMID:25057814] Peters B, et al. A Conserved cis-Regulatory Module Determines Germline Fate through Activation of the Transcription Factor DUO1 Promoter. Plant Physiol., 2017. 173(1): p. 280-293 [PMID:27624837] Zheng Z, et al. Target RNA Secondary Structure Is a Major Determinant of miR159 Efficacy. Plant Physiol., 2017. 174(3): p. 1764-1778 [PMID:28515145]