PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID Potri.009G136300.1
Common NamePOPTR_0009s13860g
Organism
Populus trichocarpa
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Malpighiales; Salicaceae; Saliceae; Populus
Family bHLH
Protein Properties Length: 586aa    MW: 66006.5 Da    PI: 4.8453
Description bHLH family protein
Gene Model
Gene Model ID Type Source Coding Sequence
Potri.009G136300.1genomeJGIView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HLH37.64.1e-12336379755
                         HHHHHHHHHHHHHHHHHHCTSCC.C...TTS-STCHHHHHHHHHHHHHHH CS
                 HLH   7 erErrRRdriNsafeeLrellPk.askapskKlsKaeiLekAveYIksLq 55 
                          +Er+RR+++N+++  Lr+l+P+ +      Kl+ a+iL  A+e++k+Lq
  Potri.009G136300.1 336 VAERKRRKKLNDRLYALRSLVPNiS------KLDRASILGDAIEFVKELQ 379
                         68*********************66......******************9 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PfamPF142157.2E-1917164IPR025610Transcription factor MYC/MYB N-terminal
PROSITE profilePS5088815.955329378IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
CDDcd000835.84E-13331381No hitNo description
SuperFamilySSF474591.57E-16332392IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SMARTSM003532.1E-15335384IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PfamPF000101.0E-9336379IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene3DG3DSA:4.10.280.102.8E-16336392IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PROSITE profilePS516718.772472545IPR002912ACT domain
CDDcd048730.00468473536No hitNo description
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0008152Biological Processmetabolic process
GO:0009555Biological Processpollen development
GO:0048657Biological Processanther wall tapetum cell differentiation
GO:0005634Cellular Componentnucleus
GO:0000978Molecular FunctionRNA polymerase II core promoter proximal region sequence-specific DNA binding
GO:0016597Molecular Functionamino acid binding
GO:0046983Molecular Functionprotein dimerization activity
Sequence ? help Back to Top
Protein Sequence    Length: 586 aa     Download sequence    Send to blast
MRYPYQPGNI MNVVQNLMER LRPLVGVKGW DYCVLWKLSD DRRYIELMDC CCAGTEATQN  60
GEELQFPVSA VLPCRDVMFQ HPGTKSCELL AQLPSSMPLN SGFHAQTLSS NLPRWLNFSS  120
SSDSNVLEET VGTRALIPVP GGLMELFIAK QVPEDQHVID VVTSQCNFLM EQEAMINSTN  180
MDSSLSIDVN VMSENQSKPF LANENEQEDH HSLNIPYDTS LDRLHMSSSP MNNFMHQFNY  240
STDETKTKGD LFQGVESGLQ DMDDLQKSMM ANAESTQMQY MESGLTTKDQ HGNDKESIKL  300
ENGPSAEYSH SDCNDDEDDA KYRRRTGKGP QSKNLVAERK RRKKLNDRLY ALRSLVPNIS  360
KLDRASILGD AIEFVKELQK EAKELQDELE ENSEDEGAKN GNNNNMPPEI LNQNGVNLGA  420
YRSDYAVNGF HVEASGISTV SKQNQDSENS HDKGHQMEAQ VEVAQIDGNE FFVKVFCEHK  480
PGGFVRLMEA LDSLGLEVTN ANVTSNRGLV SNVLKVEQKD SEMVQADYVR DSLLELTRDP  540
PRAWPEMPKA SEICCSGMDY PHHDHHQHHL QNGHMNYNHH HLHHL*
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
5gnj_A1e-123373791254Transcription factor MYC2
5gnj_B1e-123373791254Transcription factor MYC2
5gnj_E1e-123373791254Transcription factor MYC2
5gnj_F1e-123373791254Transcription factor MYC2
5gnj_G1e-123373791254Transcription factor MYC2
5gnj_I1e-123373791254Transcription factor MYC2
5gnj_M1e-123373791254Transcription factor MYC2
5gnj_N1e-123373791254Transcription factor MYC2
Search in ModeBase
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
1322343RRRTGKGPQSKNLVAERKRRKK
2337344ERKRRKKL
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
Pth.161960.0bud| catkin
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: First observed a bud stage of 0.6-mm, shortly after microspore release from the postmeiotic tetrads. Later restricted within the tapetum, microspores and anther locule. Still visible within the haploid nuclei, each of which had migrated to the pollen cell wall prior to pollen mitosis I. Not expressed later. {ECO:0000269|PubMed:12535353}.
UniprotTISSUE SPECIFICITY: Mostly expressed in closed, post-meiotic buds, and, to a lower extent, in pre-meiotic buds. Detected in leaves, stems, and flowers. {ECO:0000269|PubMed:12535353, ECO:0000269|PubMed:12679534}.
Functional Description ? help Back to Top
Source Description
UniProtTranscription factor. Plays a crucial role in tapetum development. Required for male fertility and pollen differentiation, especially during the post-meiotic transcriptional regulation of microspore development within the developing anther (PubMed:12535353). Binds E-box regions in the AHL16/TEK promoter. {ECO:0000269|PubMed:12535353, ECO:0000269|PubMed:24804694}.
Cis-element ? help Back to Top
SourceLink
PlantRegMapPotri.009G136300.1
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_024464200.10.0transcription factor ABORTED MICROSPORES isoform X1
RefseqXP_024464201.10.0transcription factor ABORTED MICROSPORES isoform X1
SwissprotQ9ZVX21e-155AMS_ARATH; Transcription factor ABORTED MICROSPORES
TrEMBLA0A2K1Z7H90.0A0A2K1Z7H9_POPTR; Uncharacterized protein
STRINGPOPTR_0009s13860.10.0(Populus trichocarpa)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
FabidsOGEF42253247
Representative plantOGRP37041323
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT2G16910.11e-157bHLH family protein
Publications ? help Back to Top
  1. Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH
    Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature.
    Differentiation, 2010. 80(1): p. 1-8
    [PMID:20219281]
  2. Jiang J,Zhang Z,Cao J
    Pollen wall development: the associated enzymes and metabolic pathways.
    Plant Biol (Stuttg), 2013. 15(2): p. 249-63
    [PMID:23252839]
  3. Xu J, et al.
    ABORTED MICROSPORES Acts as a Master Regulator of Pollen Wall Formation in Arabidopsis.
    Plant Cell, 2014. 26(4): p. 1544-1556
    [PMID:24781116]
  4. Qian H, et al.
    Trace concentrations of imazethapyr (IM) affect floral organs development and reproduction in Arabidopsis thaliana: IM-induced inhibition of key genes regulating anther and pollen biosynthesis.
    Ecotoxicology, 2015. 24(1): p. 163-71
    [PMID:25348600]
  5. Xiong SX, et al.
    The transcription factors MS188 and AMS form a complex to activate the expression of CYP703A2 for sporopollenin biosynthesis in Arabidopsis thaliana.
    Plant J., 2016. 88(6): p. 936-946
    [PMID:27460657]
  6. Ferguson AC, et al.
    Biphasic regulation of the transcription factor ABORTED MICROSPORES (AMS) is essential for tapetum and pollen development in Arabidopsis.
    New Phytol., 2017. 213(2): p. 778-790
    [PMID:27787905]
  7. Lou Y, et al.
    Positive regulation of AMS by TDF1 and the formation of a TDF1-AMS complex are required for anther development in Arabidopsis thaliana.
    New Phytol., 2018. 217(1): p. 378-391
    [PMID:28940573]
  8. Bi H,Fan W,Zhang P
    C4 Protein of Sweet Potato Leaf Curl Virus Regulates Brassinosteroid Signaling Pathway through Interaction with AtBIN2 and Affects Male Fertility in Arabidopsis.
    Front Plant Sci, 2017. 8: p. 1689
    [PMID:29021807]