PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Rosales; Rosaceae; Maloideae; Maleae; Pyrus

Family

WRKY

Protein Properties

Length: 624aa MW: 67851.3 Da PI: 6.9647

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Pbr041812.1	genome	CPETR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	96.9	1.3e-30	367	425	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 Dg++WrKYGqK+ kg+++pr+YYrCt+a gCpv+k+v+r+aed +++++tYeg+Hnh+ Pbr041812.1 367 TDGCQWRKYGQKMAKGNPCPRAYYRCTMAaGCPVRKQVQRCAEDRTILITTYEGNHNHP 425 7*************************99**************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	1.4E-33	351	427	IPR003657	WRKY domain
SuperFamily	SSF118290	1.2E-27	359	427	IPR003657	WRKY domain
PROSITE profile	PS50811	29.792	361	427	IPR003657	WRKY domain
SMART	SM00774	1.0E-36	366	426	IPR003657	WRKY domain
Pfam	PF03106	1.2E-26	368	425	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 624 aa Download sequence Send to blast
MAKGSGLSID SDPFSFSLHN PIVLNSFQED QYNHHLPPLS TTKKQLHHHH SSLMLMDNST 60 AIRRRSPTPP PPTTIQFAVN LNRSNDDDVQ HSPDSPPPSN EQRKVIDERD FFADSKNHLE 120 KSVGSDHPAD KMDLHGPTDM EFNVNTGLNL LLTNTSSDHS VAEDGIPSNI EDKRAKSELA 180 VLQAELQRIN VENQRLRGML NQVTTNYNAL QVQLLNLMQS QKADQNSSGA EGHRVALEEK 240 KLMNGNGSSL VVPRQFMDLG LAAKNGDADE HSQSSSDERS RGERSGSLGD NVKAAGHSDD 300 QGIGFDHEKK DFGKRIGREE SPDQPSQSWG PNKVPRLSSP KEVDQTEATM RKARVSVRAR 360 SEAPMITDGC QWRKYGQKMA KGNPCPRAYY RCTMAAGCPV RKQVQRCAED RTILITTYEG 420 NHNHPLPPAA MAMASTTSSA ARMLLSGAMP SADSLMDSNF LSRTILPYST SMATISASAP 480 FPTVTLDLTQ SPNPLQQFQR PPGQFNIPFP NPNQNFTSGP SSLLPQIFSQ ALYNQSKFSG 540 LQMSQDTDRA AVQLGHQSQP GQQQGSLADL TAVTAAIAAD PNFTAALAAA ITSIIGNAHP 600 NNNVANPATN SNNNNVNATK QQQ*

Sequence ? help Back to Top

Protein Sequence Length: 624 aa Download sequence Send to blast

MAKGSGLSID SDPFSFSLHN PIVLNSFQED QYNHHLPPLS TTKKQLHHHH SSLMLMDNST  60
AIRRRSPTPP PPTTIQFAVN LNRSNDDDVQ HSPDSPPPSN EQRKVIDERD FFADSKNHLE  120
KSVGSDHPAD KMDLHGPTDM EFNVNTGLNL LLTNTSSDHS VAEDGIPSNI EDKRAKSELA  180
VLQAELQRIN VENQRLRGML NQVTTNYNAL QVQLLNLMQS QKADQNSSGA EGHRVALEEK  240
KLMNGNGSSL VVPRQFMDLG LAAKNGDADE HSQSSSDERS RGERSGSLGD NVKAAGHSDD  300
QGIGFDHEKK DFGKRIGREE SPDQPSQSWG PNKVPRLSSP KEVDQTEATM RKARVSVRAR  360
SEAPMITDGC QWRKYGQKMA KGNPCPRAYY RCTMAAGCPV RKQVQRCAED RTILITTYEG  420
NHNHPLPPAA MAMASTTSSA ARMLLSGAMP SADSLMDSNF LSRTILPYST SMATISASAP  480
FPTVTLDLTQ SPNPLQQFQR PPGQFNIPFP NPNQNFTSGP SSLLPQIFSQ ALYNQSKFSG  540
LQMSQDTDRA AVQLGHQSQP GQQQGSLADL TAVTAAIAAD PNFTAALAAA ITSIIGNAHP  600
NNNVANPATN SNNNNVNATK QQQ*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
2ayd_A	9e-22	353	429	1	76	WRKY transcription factor 1
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor involved in the control of processes related to senescence and pathogen defense (PubMed:12000796). Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (PubMed:12000796). Activates the transcription of the SIRK gene and represses its own expression and that of the WRKY42 genes (PubMed:12000796). Modulates phosphate homeostasis and Pi translocation by regulating PHO1 expression (PubMed:25733771). {ECO:0000269\|PubMed:12000796, ECO:0000269\|PubMed:25733771}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Pbr041812.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By salicylic acid, ethylene, jasmonic acid, pathogens, wounding and strongly during leaf senescence. {ECO:0000269\|PubMed:11449049, ECO:0000269\|PubMed:11722756}.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_018500879.1	0.0	PREDICTED: probable WRKY transcription factor 31
Swissprot	Q9C519	1e-134	WRKY6_ARATH; WRKY transcription factor 6
TrEMBL	A0A498J3D2	0.0	A0A498J3D2_MALDO; Uncharacterized protein
STRING	XP_008342028.1	0.0	(Malus domestica)
STRING	XP_008362262.1	0.0	(Malus domestica)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Fabids	OGEF866	34	121

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT1G62300.1	1e-107	WRKY family protein

Publications ? help Back to Top
Zhou F, et al. High humidity suppresses ssi4-mediated cell death and disease resistance upstream of MAP kinase activation, H2O2 production and defense gene expression. Plant J., 2004. 39(6): p. 920-32 [PMID:15341634] Ramírez V,López A,Mauch-Mani B,Gil MJ,Vera P An extracellular subtilase switch for immune priming in Arabidopsis. PLoS Pathog., 2013. 9(6): p. e1003445 [PMID:23818851] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Singh V,Roy S,Singh D,Nandi AK Arabidopsis flowering locus D influences systemic-acquired-resistance- induced expression and histone modifications of WRKY genes. J. Biosci., 2014. 39(1): p. 119-26 [PMID:24499796] Ali MA,Wieczorek K,Kreil DP,Bohlmann H The beet cyst nematode Heterodera schachtii modulates the expression of WRKY transcription factors in syncytia to favour its development in Arabidopsis roots. PLoS ONE, 2014. 9(7): p. e102360 [PMID:25033038] Chai J,Liu J,Zhou J,Xing D Mitogen-activated protein kinase 6 regulates NPR1 gene expression and activation during leaf senescence induced by salicylic acid. J. Exp. Bot., 2014. 65(22): p. 6513-28 [PMID:25210078] Vermeirssen V,De Clercq I,Van Parys T,Van Breusegem F,Van de Peer Y Arabidopsis ensemble reverse-engineered gene regulatory network discloses interconnected transcription factors in oxidative stress. Plant Cell, 2014. 26(12): p. 4656-79 [PMID:25549671] Xiao D, et al. SENESCENCE-SUPPRESSED PROTEIN PHOSPHATASE Directly Interacts with the Cytoplasmic Domain of SENESCENCE-ASSOCIATED RECEPTOR-LIKE KINASE and Negatively Regulates Leaf Senescence in Arabidopsis. Plant Physiol., 2015. 169(2): p. 1275-91 [PMID:26304848] Bakshi M, et al. WRKY6 restricts Piriformospora indica-stimulated and phosphate-induced root development in Arabidopsis. BMC Plant Biol., 2015. 15: p. 305 [PMID:26718529] Huang Y,Feng CZ,Ye Q,Wu WH,Chen YF Arabidopsis WRKY6 Transcription Factor Acts as a Positive Regulator of Abscisic Acid Signaling during Seed Germination and Early Seedling Development. PLoS Genet., 2016. 12(2): p. e1005833 [PMID:26829043] Ramírez V,López A,Mauch-Mani B,Gil MJ,Vera P Correction: An Extracellular Subtilase Switch for Immune Priming in Arabidopsis. PLoS Pathog., 2016. 12(11): p. e1006003 [PMID:27806116] Li H, et al. BZR1 Positively Regulates Freezing Tolerance via CBF-Dependent and CBF-Independent Pathways in Arabidopsis. Mol Plant, 2017. 10(4): p. 545-559 [PMID:28089951] Stetter MG,Benz M,Ludewig U Increased root hair density by loss of WRKY6 in Arabidopsis thaliana. PeerJ, 2017. 5: p. e2891 [PMID:28149680] Zhou X, et al. StMYB44 negatively regulates phosphate transport by suppressing expression of PHOSPHATE1 in potato. J. Exp. Bot., 2017. 68(5): p. 1265-1281 [PMID:28338870] Bakshi M, et al. Piriformospora indica Reprograms Gene Expression in Arabidopsis Phosphate Metabolism Mutants But Does Not Compensate for Phosphate Limitation. Front Microbiol, 2017. 8: p. 1262 [PMID:28747898] Zhang Y, et al. DELLA proteins negatively regulate dark-induced senescence and chlorophyll degradation in Arabidopsis through interaction with the transcription factor WRKY6. Plant Cell Rep., 2018. 37(7): p. 981-992 [PMID:29574486]