PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Rosales; Rosaceae; Maloideae; Maleae; Pyrus

Family

GRAS

Protein Properties

Length: 519aa MW: 56719.4 Da PI: 5.5273

Description

GRAS family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Pbr014064.1	genome	CPETR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	GRAS	396.7	2.4e-121	200	492	79	374
GRAS 79 aalklfsevsPilkfshltaNqaIleavegeervHiiDfdisqGlQWpaLlqaLasRpegppslRiTgvgspesgskeeleetgerLakfAeelgvpf 176 +++f+e++P+lkf+h+taNqaIlea++g+++vH+iDf++++GlQWpaL+qaLa Rp+gpp lR+Tg+g+p+++ +++l+e+g rLa++A++++v+f Pbr014064.1 200 LLYHYFYEACPYLKFAHFTANQAILEAFHGHDCVHVIDFNLMHGLQWPALIQALALRPGGPPLLRLTGIGPPSPDGRDSLREIGLRLAELARSVNVRF 297 46789******************************************************************************************* PP GRAS 177 efnvlvakrledleleeLrvkpgEalaVnlvlqlhrlldesvsleserdevLklvkslsPkvvvvveqeadhnsesFlerflealeyysalfdsleak 274 f+ ++a+rled+++++L+v+p+Ea+aVn+++qlhrll ++ + +s+++ +L+ +++l+Pk+v+vveqeadhn++ Fl+rf+eal+yys++fdslea Pbr014064.1 298 AFRGVAASRLEDVKPWMLQVSPKEAVAVNSIMQLHRLLGSDPNRNSPIEMMLSWIRNLNPKIVTVVEQEADHNKPGFLDRFTEALYYYSTMFDSLEAC 395 *******************************************************************************************999 PP GRAS 275 lpreseerikvErellgreivnvvacegaerrerhetlekWrerleeaGFkpvplsekaakqaklllrkvksdgyrveeesgslvlgWkdrpLvsvSa 372 + + ++++ +++++rei+nvv+cega+r+erhe l+kWr rl++aGF+p++l+++a kqa++ll ++ +gyrvee++g+l+lgW++ pL+++Sa Pbr014064.1 396 ---AMQPEKTLAEMYIQREICNVVCCEGAARVERHEPLSKWRTRLGQAGFSPLHLGSNAFKQASMLLTLFSAEGYRVEENQGCLTLGWHSCPLIAASA 490 ...468888899999********************************************************************************* PP GRAS 373 Wr 374 W+ Pbr014064.1 491 WQ 492 *6 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Pfam	PF12041	1.8E-30	32	98	IPR021914	Transcriptional factor DELLA, N-terminal
SMART	SM01129	7.5E-19	32	104	No hit	No description
PROSITE profile	PS50985	55.613	108	472	IPR005202	Transcription factor GRAS
Pfam	PF03514	8.2E-119	200	492	IPR005202	Transcription factor GRAS

Sequence ? help Back to Top
Protein Sequence Length: 519 aa Download sequence Send to blast
MGPYGSTTCS GVSSSASSSS SSSVTKSPAE IDGYLADAGY KVRASDLRHV AQRLEHLETV 60 MVNSPSDLSQ LASDAVLYNP SDIATWVDSL LSEFNPQPLI SLPSDLDFPD GAVINNPAAL 120 AGETWTDNSS VISAVPQQNH QLTVVTAMEE DSGIRLVHLL VTCAESVQRG ELTLAGSLIE 180 TMQALMTRVN TSCGIGKNEL LYHYFYEACP YLKFAHFTAN QAILEAFHGH DCVHVIDFNL 240 MHGLQWPALI QALALRPGGP PLLRLTGIGP PSPDGRDSLR EIGLRLAELA RSVNVRFAFR 300 GVAASRLEDV KPWMLQVSPK EAVAVNSIMQ LHRLLGSDPN RNSPIEMMLS WIRNLNPKIV 360 TVVEQEADHN KPGFLDRFTE ALYYYSTMFD SLEACAMQPE KTLAEMYIQR EICNVVCCEG 420 AARVERHEPL SKWRTRLGQA GFSPLHLGSN AFKQASMLLT LFSAEGYRVE ENQGCLTLGW 480 HSCPLIAASA WQVMSMAEAT TNHQPVGILN QNNNANHV*

Sequence ? help Back to Top

Protein Sequence Length: 519 aa Download sequence Send to blast

MGPYGSTTCS GVSSSASSSS SSSVTKSPAE IDGYLADAGY KVRASDLRHV AQRLEHLETV  60
MVNSPSDLSQ LASDAVLYNP SDIATWVDSL LSEFNPQPLI SLPSDLDFPD GAVINNPAAL  120
AGETWTDNSS VISAVPQQNH QLTVVTAMEE DSGIRLVHLL VTCAESVQRG ELTLAGSLIE  180
TMQALMTRVN TSCGIGKNEL LYHYFYEACP YLKFAHFTAN QAILEAFHGH DCVHVIDFNL  240
MHGLQWPALI QALALRPGGP PLLRLTGIGP PSPDGRDSLR EIGLRLAELA RSVNVRFAFR  300
GVAASRLEDV KPWMLQVSPK EAVAVNSIMQ LHRLLGSDPN RNSPIEMMLS WIRNLNPKIV  360
TVVEQEADHN KPGFLDRFTE ALYYYSTMFD SLEACAMQPE KTLAEMYIQR EICNVVCCEG  420
AARVERHEPL SKWRTRLGQA GFSPLHLGSN AFKQASMLLT LFSAEGYRVE ENQGCLTLGW  480
HSCPLIAASA WQVMSMAEAT TNHQPVGILN QNNNANHV*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
5b3g_A	2e-58	202	491	99	378	Protein SCARECROW
5b3h_A	2e-58	202	491	98	377	Protein SCARECROW
5b3h_D	2e-58	202	491	98	377	Protein SCARECROW
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Probably acts by participating in large multiprotein complexes that repress transcription of GA-inducible genes. Upon GA application, it is degraded by the proteasome, allowing the GA signaling pathway.
UniProt	Transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Transcription coactivator of the zinc finger transcription factors GAF1/IDD2 and ENY/IDD1 in regulation of gibberellin homeostasis and signaling (PubMed:25035403). No effect of the BOI proteins on its stability. Probably acts by participating in large multiprotein complexes that repress transcription of GA-inducible genes. Positively regulates XERICO expression. In contrast to RGA, it is less sensitive to GA. Its activity is probably regulated by other phytohormones such as auxin and ethylene. {ECO:0000269\|PubMed:11487693, ECO:0000269\|PubMed:11606551, ECO:0000269\|PubMed:11606552, ECO:0000269\|PubMed:14973286, ECO:0000269\|PubMed:15128937, ECO:0000269\|PubMed:16034591, ECO:0000269\|PubMed:17933900, ECO:0000269\|PubMed:25035403, ECO:0000269\|PubMed:9389651}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Pbr014064.1

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	DQ007888	0.0	DQ007888.1 Malus x domestica DELLA protein (L3b) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_009371410.1	0.0	PREDICTED: DELLA protein GAI-like
Swissprot	Q8S4W7	1e-159	GAI1_VITVI; DELLA protein GAI1
Swissprot	Q9LQT8	1e-159	GAI_ARATH; DELLA protein GAI
TrEMBL	A0A192ADJ4	0.0	A0A192ADJ4_PYRPY; DELLA protein GAI3
STRING	XP_009371410.1	0.0	(Pyrus x bretschneideri)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Fabids	OGEF803	34	121

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT1G14920.1	1e-141	GRAS family protein

Publications ? help Back to Top
Duarte JM, et al. Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. Mol. Biol. Evol., 2006. 23(2): p. 469-78 [PMID:16280546] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Gallego-Giraldo C, et al. Role of the gibberellin receptors GID1 during fruit-set in Arabidopsis. Plant J., 2014. 79(6): p. 1020-1032 [PMID:24961590] Fukazawa J, et al. DELLAs function as coactivators of GAI-ASSOCIATED FACTOR1 in regulation of gibberellin homeostasis and signaling in Arabidopsis. Plant Cell, 2014. 26(7): p. 2920-38 [PMID:25035403] Marín-de la Rosa N, et al. Genome Wide Binding Site Analysis Reveals Transcriptional Coactivation of Cytokinin-Responsive Genes by DELLA Proteins. PLoS Genet., 2015. 11(7): p. e1005337 [PMID:26134422] Shi H,Wei Y,Wang Q,Reiter RJ,He C Melatonin mediates the stabilization of DELLA proteins to repress the floral transition in Arabidopsis. J. Pineal Res., 2016. 60(3): p. 373-9 [PMID:26887824] Lee SA, et al. Interplay between ABA and GA Modulates the Timing of Asymmetric Cell Divisions in the Arabidopsis Root Ground Tissue. Mol Plant, 2016. 9(6): p. 870-84 [PMID:26970019] Qu J,Kang SG,Hah C,Jang JC Molecular and cellular characterization of GA-Stimulated Transcripts GASA4 and GASA6 in Arabidopsis thaliana. Plant Sci., 2016. 246: p. 1-10 [PMID:26993231] Shahnejat-Bushehri S,Nobmann B,Devi Allu A,Balazadeh S JUB1 suppresses Pseudomonas syringae-induced defense responses through accumulation of DELLA proteins. Plant Signal Behav, 2016. 11(6): p. e1181245 [PMID:27159137] Shahnejat-Bushehri S,Tarkowska D,Sakuraba Y,Balazadeh S Arabidopsis NAC transcription factor JUB1 regulates GA/BR metabolism and signalling. Nat Plants, 2016. 2: p. 16013 [PMID:27249348] Wang H, et al. The DELLA-CONSTANS Transcription Factor Cascade Integrates Gibberellic Acid and Photoperiod Signaling to Regulate Flowering. Plant Physiol., 2016. 172(1): p. 479-88 [PMID:27406167] Li W,Wang H,Yu D Arabidopsis WRKY Transcription Factors WRKY12 and WRKY13 Oppositely Regulate Flowering under Short-Day Conditions. Mol Plant, 2016. 9(11): p. 1492-1503 [PMID:27592586] Liu B,De Storme N,Geelen D Gibberellin Induces Diploid Pollen Formation by Interfering with Meiotic Cytokinesis. Plant Physiol., 2017. 173(1): p. 338-353 [PMID:27621423] Matsuoka K, et al. Differential Cellular Control by Cotyledon-Derived Phytohormones Involved in Graft Reunion of Arabidopsis Hypocotyls. Plant Cell Physiol., 2016. 57(12): p. 2620-2631 [PMID:27986917] Zentella R, et al. The Arabidopsis O-fucosyltransferase SPINDLY activates nuclear growth repressor DELLA. Nat. Chem. Biol., 2017. 13(5): p. 479-485 [PMID:28244988] Zhang Y, et al. GA-DELLA pathway is involved in regulation of nitrogen deficiency-induced anthocyanin accumulation. Plant Cell Rep., 2017. 36(4): p. 557-569 [PMID:28275852] Zhang L,Chen L,Yu D Transcription Factor WRKY75 Interacts with DELLA Proteins to Affect Flowering. Plant Physiol., 2018. 176(1): p. 790-803 [PMID:29133369] Nelson SK,Ariizumi T,Steber CM Biology in the Dry Seed: Transcriptome Changes Associated with Dry Seed Dormancy and Dormancy Loss in the Arabidopsis GA-Insensitive sleepy1-2 Mutant. Front Plant Sci, 2017. 8: p. 2158 [PMID:29312402] Liu B,De Storme N,Geelen D Cold-Induced Male Meiotic Restitution in Arabidopsis thaliana Is Not Mediated by GA-DELLA Signaling. Front Plant Sci, 2018. 9: p. 91 [PMID:29459879] Zhang Y, et al. DELLA proteins negatively regulate dark-induced senescence and chlorophyll degradation in Arabidopsis through interaction with the transcription factor WRKY6. Plant Cell Rep., 2018. 37(7): p. 981-992 [PMID:29574486] Felipo-Benavent A, et al. Regulation of xylem fiber differentiation by gibberellins through DELLA-KNAT1 interaction. Development, 2019. [PMID:30389856] Wright DA, et al. Recovery of YAC-end sequences through complementation of an Escherichia coli pyrF mutation. Nucleic Acids Res., 1997. 25(13): p. 2679-80 [PMID:9185581]