PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae; Nicotiana

Family

ARF

Protein Properties

Length: 733aa MW: 81002.6 Da PI: 6.3347

Description

ARF family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Niben101Scf00152g20002.1	genome	BTI	-

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	B3	78.4	7.5e-25	146	247	1	99
EEEE-..-HHHHTT-EE--HHH.HTT......---..--SEEEEEETTS-EEEEEE..EEETTEEEE-TTHHHHHHHHT--TT-E CS B3 1 ffkvltpsdvlksgrlvlpkkfaeeh......ggkkeesktltledesgrsWevkliyrkksgryvltkGWkeFvkangLkegDf 79 f+k+lt sd++++g +++p++ ae+ ++++++ ++l+++d++ ++W++++iyr++++r++lt+GW+ Fv a++L++gD+ Niben101Scf00152g20002.1 146 FCKTLTASDTSTHGGFSVPRRAAEKLfppldySMQPPT-QELVVRDLHDNTWTFRHIYRGQPKRHLLTTGWSMFVGAKRLRAGDS 229 99*******************999*9555554.49**************************************** PP EEEEE-SSSEE..EEEEE-S CS B3 80 vvFkldgrsefelvvkvfrk 99 v+F ++++ +l+++v+r+ Niben101Scf00152g20002.1 230 VLFI--RDEKSQLMLGVRRA 247 ..4567778*9997 PP
2	Auxin_resp	118.8	4.3e-39	272	355	1	83
Auxin_resp 1 aahaastksvFevvYnPrastseFvvkvekvekalk.vkvsvGmRfkmafetedsserrlsGtvvgvsdldpvrWpnSkWrsLk 83 aahaa+++s+F+++YnPra++seFv++++k++k+++ +++svGmRf m+fete+s +rr++Gt+vg+sdldp+rWp+SkWr+L+ Niben101Scf00152g20002.1 272 AAHAAANRSTFTIFYNPRACPSEFVIPLAKYRKSVYgTQLSVGMRFGMMFETEESGKRRYMGTIVGISDLDPLRWPGSKWRCLQ 355 79********************************9********************************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SuperFamily	SSF101936	1.44E-47	133	275	IPR015300	DNA-binding pseudobarrel domain
Gene3D	G3DSA:2.40.330.10	1.7E-42	139	260	IPR015300	DNA-binding pseudobarrel domain
CDD	cd10017	2.57E-23	145	246	No hit	No description
Pfam	PF02362	2.0E-22	146	247	IPR003340	B3 DNA binding domain
PROSITE profile	PS50863	13.267	146	248	IPR003340	B3 DNA binding domain
SMART	SM01019	7.0E-25	146	248	IPR003340	B3 DNA binding domain
Pfam	PF06507	5.4E-34	272	355	IPR010525	Auxin response factor
PROSITE profile	PS51745	21.39	620	704	IPR000270	PB1 domain
Pfam	PF02309	2.4E-8	631	706	IPR033389	AUX/IAA domain
SuperFamily	SSF54277	9.16E-9	635	699	No hit	No description

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009725	Biological Process	response to hormone
GO:0005634	Cellular Component	nucleus
GO:0003677	Molecular Function	DNA binding
GO:0005515	Molecular Function	protein binding

Sequence ? help Back to Top
Protein Sequence Length: 733 aa Download sequence Send to blast
MGSVEEKHKP GSLVSGAHTL LEEMKLLKEM QDHSGGGKLI NSELWHACAG PLVTLPQVGS 60 LVYYFPQGHS EQVAVSTNRT ATSQIPNYPN LASQLLCQVH NVTLHADKET DEIYAQMSLQ 120 PVNSERDVFP IPDFGLKPSK HPTEFFCKTL TASDTSTHGG FSVPRRAAEK LFPPLDYSMQ 180 PPTQELVVRD LHDNTWTFRH IYRGQPKRHL LTTGWSMFVG AKRLRAGDSV LFIRDEKSQL 240 MLGVRRANRQ QTSLPSSVLS ADSMHIGVLA AAAHAAANRS TFTIFYNPRA CPSEFVIPLA 300 KYRKSVYGTQ LSVGMRFGMM FETEESGKRR YMGTIVGISD LDPLRWPGSK WRCLQAEWDE 360 PGCGDKQSRV SPWEVETPES LFIFPSLTAG LKRPYQSSFL GGQTEWDSLM HRPFMRVPEN 420 VYGELQSSSI SNLWSEQLMK MLARPPGVTG LQWGVPTVQD IKVALPQESR NLIHAAGNQK 480 PELSTVEDTS VQSERNPLVI LNQPCVVNSI SSSQATLQPK SQPPEKVGSD TVGISTEPAK 540 ETSVSVDRLD QCPSLGQCSE EKVTIKPASP LILPTDATAS HQNSLSQLQS SPWRQIESAS 600 ANNILQGSNN NEWNLPSFQS TAGLIQKAGS VGRSIDVSSF KTYHELRSEI ERMFGLEGLL 660 NDTKGSGWKL VYVDYENDVL LVGDDPWEEF VGCVRCIRIL SPTEVQQMGE EGMQLLNSAG 720 LQGINGSTSE FPN

Sequence ? help Back to Top

Protein Sequence Length: 733 aa Download sequence Send to blast

MGSVEEKHKP GSLVSGAHTL LEEMKLLKEM QDHSGGGKLI NSELWHACAG PLVTLPQVGS  60
LVYYFPQGHS EQVAVSTNRT ATSQIPNYPN LASQLLCQVH NVTLHADKET DEIYAQMSLQ  120
PVNSERDVFP IPDFGLKPSK HPTEFFCKTL TASDTSTHGG FSVPRRAAEK LFPPLDYSMQ  180
PPTQELVVRD LHDNTWTFRH IYRGQPKRHL LTTGWSMFVG AKRLRAGDSV LFIRDEKSQL  240
MLGVRRANRQ QTSLPSSVLS ADSMHIGVLA AAAHAAANRS TFTIFYNPRA CPSEFVIPLA  300
KYRKSVYGTQ LSVGMRFGMM FETEESGKRR YMGTIVGISD LDPLRWPGSK WRCLQAEWDE  360
PGCGDKQSRV SPWEVETPES LFIFPSLTAG LKRPYQSSFL GGQTEWDSLM HRPFMRVPEN  420
VYGELQSSSI SNLWSEQLMK MLARPPGVTG LQWGVPTVQD IKVALPQESR NLIHAAGNQK  480
PELSTVEDTS VQSERNPLVI LNQPCVVNSI SSSQATLQPK SQPPEKVGSD TVGISTEPAK  540
ETSVSVDRLD QCPSLGQCSE EKVTIKPASP LILPTDATAS HQNSLSQLQS SPWRQIESAS  600
ANNILQGSNN NEWNLPSFQS TAGLIQKAGS VGRSIDVSSF KTYHELRSEI ERMFGLEGLL  660
NDTKGSGWKL VYVDYENDVL LVGDDPWEEF VGCVRCIRIL SPTEVQQMGE EGMQLLNSAG  720
LQGINGSTSE FPN

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
4ldu_A	0.0	1	380	5	392	Auxin response factor 5
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Seems to act as transcriptional activator. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Mediates embryo axis formation and vascular tissues differentiation. Functionally redundant with ARF7. May be necessary to counteract AMP1 activity. {ECO:0000269\|PubMed:12036261, ECO:0000269\|PubMed:14973283, ECO:0000269\|PubMed:17553903}.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_019263965.1	0.0	PREDICTED: auxin response factor 5
Swissprot	P93024	0.0	ARFE_ARATH; Auxin response factor 5
TrEMBL	A0A314L7C1	0.0	A0A314L7C1_NICAT; Auxin response factor
STRING	XP_009778804.1	0.0	(Nicotiana sylvestris)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Asterids	OGEA5859	23	34

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT1G19850.1	0.0	ARF family protein

Publications ? help Back to Top
Chen MK,Wilson RL,Palme K,Ditengou FA,Shpak ED ERECTA family genes regulate auxin transport in the shoot apical meristem and forming leaf primordia. Plant Physiol., 2013. 162(4): p. 1978-91 [PMID:23821653] Huang JB, et al. ROP3 GTPase contributes to polar auxin transport and auxin responses and is important for embryogenesis and seedling growth in Arabidopsis. Plant Cell, 2014. 26(9): p. 3501-18 [PMID:25217509] Qi J, et al. Auxin depletion from leaf primordia contributes to organ patterning. Proc. Natl. Acad. Sci. U.S.A., 2014. 111(52): p. 18769-74 [PMID:25512543] Crawford BC, et al. Plant development. Genetic control of distal stem cell fate within root and embryonic meristems. Science, 2015. 347(6222): p. 655-9 [PMID:25612610] Zhang Y, et al. Regulation of oncogene expression in T-DNA-transformed host plant cells. PLoS Pathog., 2015. 11(1): p. e1004620 [PMID:25615824] Robert HS, et al. Plant embryogenesis requires AUX/LAX-mediated auxin influx. Development, 2015. 142(4): p. 702-11 [PMID:25617434] Krogan NT,Berleth T The identification and characterization of specific ARF-Aux/IAA regulatory modules in plant growth and development. Plant Signal Behav, 2015. 10(4): p. e992748 [PMID:25830553] Ckurshumova W,Berleth T Overcoming recalcitrance - Auxin response factor functions in plant regeneration. Plant Signal Behav, 2015. 10(7): p. e993293 [PMID:26098229] Wendrich JR, et al. A set of domain-specific markers in the Arabidopsis embryo. Plant Reprod, 2015. 28(3-4): p. 153-60 [PMID:26216537] Wu MF, et al. Auxin-regulated chromatin switch directs acquisition of flower primordium founder fate. Elife, 2015. 4: p. e09269 [PMID:26460543] Yamaguchi N,Jeong CW,Nole-Wilson S,Krizek BA,Wagner D AINTEGUMENTA and AINTEGUMENTA-LIKE6/PLETHORA3 Induce LEAFY Expression in Response to Auxin to Promote the Onset of Flower Formation in Arabidopsis. Plant Physiol., 2016. 170(1): p. 283-93 [PMID:26537561] Müller CJ, et al. PHABULOSA Mediates an Auxin Signaling Loop to Regulate Vascular Patterning in Arabidopsis. Plant Physiol., 2016. 170(2): p. 956-70 [PMID:26637548] Herud O,Weijers D,Lau S,Jürgens G Auxin responsiveness of the MONOPTEROS-BODENLOS module in primary root initiation critically depends on the nuclear import kinetics of the Aux/IAA inhibitor BODENLOS. Plant J., 2016. 85(2): p. 269-77 [PMID:26714008] Luo S, et al. Constitutive Expression of OsIAA9 Affects Starch Granules Accumulation and Root Gravitropic Response in Arabidopsis. Front Plant Sci, 2015. 6: p. 1156 [PMID:26734051] Yamaguchi N,Wu MF,Winter CM,Wagner D LEAFY and Polar Auxin Transport Coordinately Regulate Arabidopsis Flower Development. Plants (Basel), 2014. 3(2): p. 251-65 [PMID:27135503] Krogan NT,Marcos D,Weiner AI,Berleth T The auxin response factor MONOPTEROS controls meristem function and organogenesis in both the shoot and root through the direct regulation of PIN genes. New Phytol., 2016. 212(1): p. 42-50 [PMID:27441727] Fendrych M,Leung J,Friml J TIR1/AFB-Aux/IAA auxin perception mediates rapid cell wall acidification and growth of Arabidopsis hypocotyls. Elife, 2018. [PMID:27627746] Matthes M,Torres-Ruiz RA Boronic acid treatment phenocopies monopteros by affecting PIN1 membrane stability and polar auxin transport in Arabidopsis thaliana embryos. Development, 2016. 143(21): p. 4053-4062 [PMID:27697905] Bhatia N, et al. Auxin Acts through MONOPTEROS to Regulate Plant Cell Polarity and Pattern Phyllotaxis. Curr. Biol., 2016. 26(23): p. 3202-3208 [PMID:27818174] Wójcikowska B,Gaj MD Expression profiling of AUXIN RESPONSE FACTOR genes during somatic embryogenesis induction in Arabidopsis. Plant Cell Rep., 2017. 36(6): p. 843-858 [PMID:28255787] Möller BK, et al. Auxin response cell-autonomously controls ground tissue initiation in the early Arabidopsis embryo. Proc. Natl. Acad. Sci. U.S.A., 2017. 114(12): p. E2533-E2539 [PMID:28265057] Carey NS,Krogan NT The role of AUXIN RESPONSE FACTORs in the development and differential growth of inflorescence stems. Plant Signal Behav, 2017. 12(4): p. e1307492 [PMID:28340328] Guan C, et al. Spatial Auxin Signaling Controls Leaf Flattening in Arabidopsis. Curr. Biol., 2017. 27(19): p. 2940-2950.e4 [PMID:28943086] Gaillochet C, et al. Control of plant cell fate transitions by transcriptional and hormonal signals. Elife, 2018. [PMID:29058667] Zheng K, et al. Involvement of PACLOBUTRAZOL RESISTANCE6/KIDARI, an Atypical bHLH Transcription Factor, in Auxin Responses in Arabidopsis. Front Plant Sci, 2017. 8: p. 1813 [PMID:29114256] Liu Z, et al. ARF2-ARF4 and ARF5 are Essential for Female and Male Gametophyte Development in Arabidopsis. Plant Cell Physiol., 2018. 59(1): p. 179-189 [PMID:29145642]