PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; stem eudicotyledons; Proteales; Nelumbonaceae; Nelumbo

Family

WRKY

Protein Properties

Length: 576aa MW: 62358.8 Da PI: 6.6013

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
NNU_000764-RA	genome	CAS	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	94.6	6.8e-30	227	286	1	59
---SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 1 ldDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 ++Dg++WrKYGqK +g+++pr+YYrCt a Cpv+k+v+r+aed +++++tYeg+Hnh+ NNU_000764-RA 227 MNDGCQWRKYGQKIARGNPCPRAYYRCTVApACPVRKQVQRCAEDMSILITTYEGTHNHP 286 58*************************99**************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	2.9E-31	212	288	IPR003657	WRKY domain
SuperFamily	SSF118290	9.81E-27	220	288	IPR003657	WRKY domain
PROSITE profile	PS50811	28.231	222	288	IPR003657	WRKY domain
SMART	SM00774	1.8E-35	227	287	IPR003657	WRKY domain
Pfam	PF03106	2.6E-26	228	286	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0006952	Biological Process	defense response
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 576 aa Download sequence Send to blast
MAHGSPAKLH EFSRWVTNSL SSETRIPTVR TEMSSMETIL RASSTIKKDQ EDPLESAKAE 60 MGEVREENER LKMILSRIVK DYQSLQMQFY GIVEQEEAKK SGDTAPVDQE IEEPELVSLS 120 LGRTASEPKK DEKNSNSSKS KEDDDQEGLS LGLDCKFEVS KPASTEKASN PSQENSFEEP 180 KEEDAGETWP PSKILKAMRS GDDEVSQQTH VKKARVSVRA RCDTPTMNDG CQWRKYGQKI 240 ARGNPCPRAY YRCTVAPACP VRKQVQRCAE DMSILITTYE GTHNHPLPVS ATAMASTTSA 300 AACMLMSGSS SSRSIPGSSA TNTTSADLHG LNFNLGDNTR ARPSFYLPNS SISSATSHPT 360 ITLDLTAPPS SSASSSSQNN RLSPNFPVQR YSSTSFNLSS TEPNNSQLTW GNGYLSYGAQ 420 PYTKNQQLGP LNLGRQPQPQ EHFYQSYMQK NNPAPSQQSL TETIAAATKA ITSDPSFRSA 480 LAAAITSIVG GGSTGAQGNQ GGCENFGQNL KWGENINTIT SYTSTPNGNG CASSYLNRSS 540 SSNTQQGSLM ILPPALPFST SKSASAFPAD TRDHIN

Sequence ? help Back to Top

Protein Sequence Length: 576 aa Download sequence Send to blast

MAHGSPAKLH EFSRWVTNSL SSETRIPTVR TEMSSMETIL RASSTIKKDQ EDPLESAKAE  60
MGEVREENER LKMILSRIVK DYQSLQMQFY GIVEQEEAKK SGDTAPVDQE IEEPELVSLS  120
LGRTASEPKK DEKNSNSSKS KEDDDQEGLS LGLDCKFEVS KPASTEKASN PSQENSFEEP  180
KEEDAGETWP PSKILKAMRS GDDEVSQQTH VKKARVSVRA RCDTPTMNDG CQWRKYGQKI  240
ARGNPCPRAY YRCTVAPACP VRKQVQRCAE DMSILITTYE GTHNHPLPVS ATAMASTTSA  300
AACMLMSGSS SSRSIPGSSA TNTTSADLHG LNFNLGDNTR ARPSFYLPNS SISSATSHPT  360
ITLDLTAPPS SSASSSSQNN RLSPNFPVQR YSSTSFNLSS TEPNNSQLTW GNGYLSYGAQ  420
PYTKNQQLGP LNLGRQPQPQ EHFYQSYMQK NNPAPSQQSL TETIAAATKA ITSDPSFRSA  480
LAAAITSIVG GGSTGAQGNQ GGCENFGQNL KWGENINTIT SYTSTPNGNG CASSYLNRSS  540
SSNTQQGSLM ILPPALPFST SKSASAFPAD TRDHIN

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
2ayd_A	4e-20	214	288	1	74	WRKY transcription factor 1
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription activator involved in the transcriptional regulation of terpene biosynthesis in glandular trichomes (PubMed:24884371). Binds to the promoter of the linalool synthase TPS5 and promotes TPS5 gene transactivation (PubMed:24884371). In association with WRKY72B, contributes to basal defense against root-knot nematodes (RKNs) and potato aphids, as well as Mi-1-mediated gene-for-gene resistance to these pests (PubMed:20409007). Both WRKY72A and WRKY72B are not required for gene-for-gene resistance mediated by Pto, another tomato R gene (PubMed:20409007). {ECO:0000269\|PubMed:20409007, ECO:0000269\|PubMed:24884371}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Induced by infection with the root-knot nematode (RKN) Meloidogyne incognita and potato aphids. {ECO:0000269\|PubMed:20409007}.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_010256554.1	0.0	PREDICTED: probable WRKY transcription factor 72 isoform X1
Swissprot	A0A3Q7GFB5	1e-129	WK72A_SOLLC; WRKY transcription factor 72A
TrEMBL	A0A1U7ZQR0	0.0	A0A1U7ZQR0_NELNU; probable WRKY transcription factor 72 isoform X1
STRING	XP_010256554.1	0.0	(Nelumbo nucifera)

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT5G15130.1	2e-79	WRKY DNA-binding protein 72

Publications ? help Back to Top
Tomato Genome Consortium The tomato genome sequence provides insights into fleshy fruit evolution. Nature, 2012. 485(7400): p. 635-41 [PMID:22660326] Spyropoulou EA,Haring MA,Schuurink RC RNA sequencing on Solanum lycopersicum trichomes identifies transcription factors that activate terpene synthase promoters. BMC Genomics, 2014. 15: p. 402 [PMID:24884371]