PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Malpighiales; Euphorbiaceae; Crotonoideae; Manihoteae; Manihot

Family

HSF

Protein Properties

Length: 420aa MW: 47329.8 Da PI: 4.8274

Description

HSF family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Manes.03G035500.1.p	genome	JGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HSF_DNA-bind	114.9	5.1e-36	100	191	2	102
HHHHHHHHHCTGGGTTTSEESSSSSEEEES-HHHHHHHTHHHHSTT--HHHHHHHHHHTTEEE---SSBTTTTXTTSEEEEESXXXXXXX CS HSF_DNA-bind 2 FlkklyeiledeelkeliswsengnsfvvldeeefakkvLpkyFkhsnfaSFvRQLnmYgFkkvkdeekkskskekiweFkhksFkkgkk 91 Flkk+++++ed+e+++++sws+ng+sf+v+d +ef+k++LpkyFkh nf+SF+RQLn+YgF+k++ ++ weF+++ F+ g+k Manes.03G035500.1.p 100 FLKKTFQMVEDPETDSTVSWSKNGDSFIVWDAHEFSKTLLPKYFKHCNFSSFIRQLNTYGFRKIDPDR---------WEFANEGFRGGEK 180 9**************************************************************999.........********* PP XXXXXXXXXXX CS HSF_DNA-bind 92 ellekikrkks 102 +ll++ikr+++ Manes.03G035500.1.p 181 HLLKNIKRRSR 191 ******986 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:1.10.10.10	5.2E-39	96	189	IPR011991	Winged helix-turn-helix DNA-binding domain
SuperFamily	SSF46785	1.63E-35	96	189	IPR011991	Winged helix-turn-helix DNA-binding domain
SMART	SM00415	3.9E-59	96	189	IPR000232	Heat shock factor (HSF)-type, DNA-binding
Pfam	PF00447	4.9E-32	100	189	IPR000232	Heat shock factor (HSF)-type, DNA-binding
PRINTS	PR00056	4.9E-19	100	123	IPR000232	Heat shock factor (HSF)-type, DNA-binding
PRINTS	PR00056	4.9E-19	138	150	IPR000232	Heat shock factor (HSF)-type, DNA-binding
PROSITE pattern	PS00434	0	139	163	IPR000232	Heat shock factor (HSF)-type, DNA-binding
PRINTS	PR00056	4.9E-19	151	163	IPR000232	Heat shock factor (HSF)-type, DNA-binding

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0005634	Cellular Component	nucleus
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 420 aa Download sequence Send to blast
MPVVRQSISL NSAAFGEATH GGLPSSSSSS INTPEIQKPA VNDTVNVITI KSEDEEEYTG 60 AIGFGDEFEG VNEDGSSSSS FSVDLPKSME GLHDTGPPPF LKKTFQMVED PETDSTVSWS 120 KNGDSFIVWD AHEFSKTLLP KYFKHCNFSS FIRQLNTYGF RKIDPDRWEF ANEGFRGGEK 180 HLLKNIKRRS RYNKQQQGKA IGDDSPKSDL EAELENLKND QDLLRIEILK LRQQRENSDN 240 QLSIVEQHIQ VAECKQLQMF IFLNKAAKNR AFIQNLIQKR KQQRELGGSA FKKRRSLSTQ 300 IPKSLPDAAD ASQSVNCRHQ AQEQLAAMQT ELTEVLREDT ETNTMSKLIE QPMSDELYSP 360 IDDQKASLIC GTNDRETVYH LMSEKLLDDN AGSENLVEQD LEVNETKLYL ELENLIGKP*

Sequence ? help Back to Top

Protein Sequence Length: 420 aa Download sequence Send to blast

MPVVRQSISL NSAAFGEATH GGLPSSSSSS INTPEIQKPA VNDTVNVITI KSEDEEEYTG  60
AIGFGDEFEG VNEDGSSSSS FSVDLPKSME GLHDTGPPPF LKKTFQMVED PETDSTVSWS  120
KNGDSFIVWD AHEFSKTLLP KYFKHCNFSS FIRQLNTYGF RKIDPDRWEF ANEGFRGGEK  180
HLLKNIKRRS RYNKQQQGKA IGDDSPKSDL EAELENLKND QDLLRIEILK LRQQRENSDN  240
QLSIVEQHIQ VAECKQLQMF IFLNKAAKNR AFIQNLIQKR KQQRELGGSA FKKRRSLSTQ  300
IPKSLPDAAD ASQSVNCRHQ AQEQLAAMQT ELTEVLREDT ETNTMSKLIE QPMSDELYSP  360
IDDQKASLIC GTNDRETVYH LMSEKLLDDN AGSENLVEQD LEVNETKLYL ELENLIGKP*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
5d5u_B	4e-25	75	189	4	129	Heat shock factor protein 1
5d5v_B	4e-25	75	189	4	129	Heat shock factor protein 1
5d5v_D	4e-25	75	189	4	129	Heat shock factor protein 1
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Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	182	188	LKNIKRR
2	278	294	KRKQQRELGGSAFKKRR

Functional Description ? help Back to Top
Source	Description
UniProt	Transcriptional activator that specifically binds DNA sequence 5'-AGAAnnTTCT-3' known as heat shock promoter elements (HSE). Involved in heat stress responses. Seems to be involved in other environmental stress responses. Activates ascorbate peroxidase 2 (APX2) in addition to several heat shock protein (HSPs). {ECO:0000269\|PubMed:16649111, ECO:0000269\|PubMed:17059409, ECO:0000269\|PubMed:17085506}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Manes.03G035500.1.p

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By heat stress, high light and hydrogen peroxide (H(2)O(2)). {ECO:0000269\|PubMed:16649111, ECO:0000269\|PubMed:17059409, ECO:0000269\|PubMed:17085506}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_021606522.1	0.0	heat shock factor protein HSF30-like
Swissprot	O80982	4e-77	HSFA2_ARATH; Heat stress transcription factor A-2
TrEMBL	A0A2C9W4A2	0.0	A0A2C9W4A2_MANES; Uncharacterized protein
STRING	cassava4.1_008446m	0.0	(Manihot esculenta)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Fabids	OGEF10676	29	38

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT2G26150.1	3e-79	heat shock transcription factor A2

Link Out ? help Back to Top
Phytozome	Manes.03G035500.1.p

Publications ? help Back to Top
Liu HC,Charng YY Common and distinct functions of Arabidopsis class A1 and A2 heat shock factors in diverse abiotic stress responses and development. Plant Physiol., 2013. 163(1): p. 276-90 [PMID:23832625] Jung HS, et al. Subset of heat-shock transcription factors required for the early response of Arabidopsis to excess light. Proc. Natl. Acad. Sci. U.S.A., 2013. 110(35): p. 14474-9 [PMID:23918368] Chauhan H,Khurana N,Agarwal P,Khurana JP,Khurana P A seed preferential heat shock transcription factor from wheat provides abiotic stress tolerance and yield enhancement in transgenic Arabidopsis under heat stress environment. PLoS ONE, 2013. 8(11): p. e79577 [PMID:24265778] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Weng M, et al. Histone chaperone ASF1 is involved in gene transcription activation in response to heat stress in Arabidopsis thaliana. Plant Cell Environ., 2014. 37(9): p. 2128-38 [PMID:24548003] Jang YH, et al. A homolog of splicing factor SF1 is essential for development and is involved in the alternative splicing of pre-mRNA in Arabidopsis thaliana. Plant J., 2014. 78(4): p. 591-603 [PMID:24580679] Stief A, et al. Arabidopsis miR156 Regulates Tolerance to Recurring Environmental Stress through SPL Transcription Factors. Plant Cell, 2014. 26(4): p. 1792-1807 [PMID:24769482] Dobrá J, et al. The impact of heat stress targeting on the hormonal and transcriptomic response in Arabidopsis. Plant Sci., 2015. 231: p. 52-61 [PMID:25575991] Yamauchi Y,Kunishima M,Mizutani M,Sugimoto Y Reactive short-chain leaf volatiles act as powerful inducers of abiotic stress-related gene expression. Sci Rep, 2015. 5: p. 8030 [PMID:25619826] Shi H, et al. Melatonin induces class A1 heat-shock factors (HSFA1s) and their possible involvement of thermotolerance in Arabidopsis. J. Pineal Res., 2015. 58(3): p. 335-42 [PMID:25711624] Mueller SP,Krause DM,Mueller MJ,Fekete A Accumulation of extra-chloroplastic triacylglycerols in Arabidopsis seedlings during heat acclimation. J. Exp. Bot., 2015. 66(15): p. 4517-26 [PMID:25977236] Nie S,Yue H,Xing D A Potential Role for Mitochondrial Produced Reactive Oxygen Species in Salicylic Acid-Mediated Plant Acquired Thermotolerance. Plant Physiol., 2016. [PMID:26099269] Nguyen AH, et al. Loss of Arabidopsis 5'-3' Exoribonuclease AtXRN4 Function Enhances Heat Stress Tolerance of Plants Subjected to Severe Heat Stress. Plant Cell Physiol., 2015. 56(9): p. 1762-72 [PMID:26136597] Hu Z, et al. Histone acetyltransferase GCN5 is essential for heat stress-responsive gene activation and thermotolerance in Arabidopsis. Plant J., 2015. 84(6): p. 1178-91 [PMID:26576681] Lämke J,Brzezinka K,Bäurle I HSFA2 orchestrates transcriptional dynamics after heat stress in Arabidopsis thaliana. Transcription, 2016. 7(4): p. 111-4 [PMID:27383578] Wang X,Huang W,Liu J,Yang Z,Huang B Molecular regulation and physiological functions of a novel FaHsfA2c cloned from tall fescue conferring plant tolerance to heat stress. Plant Biotechnol. J., 2017. 15(2): p. 237-248 [PMID:27500592] Chen ST,He NY,Chen JH,Guo FQ Identification of core subunits of photosystem II as action sites of HSP21, which is activated by the GUN5-mediated retrograde pathway in Arabidopsis. Plant J., 2017. 89(6): p. 1106-1118 [PMID:27943531] Kataoka R,Takahashi M,Suzuki N Coordination between bZIP28 and HSFA2 in the regulation of heat response signals in Arabidopsis. Plant Signal Behav, 2017. 12(11): p. e1376159 [PMID:28873003] Wang X,Zhuang L,Shi Y,Huang B Up-Regulation of HSFA2c and HSPs by ABA Contributing to Improved Heat Tolerance in Tall Fescue and Arabidopsis. Int J Mol Sci, 2018. [PMID:28914758] Llamas E,Pulido P,Rodriguez-Concepcion M Interference with plastome gene expression and Clp protease activity in Arabidopsis triggers a chloroplast unfolded protein response to restore protein homeostasis. PLoS Genet., 2017. 13(9): p. e1007022 [PMID:28937985] Li X, et al. Plastid Translation Elongation Factor Tu Is Prone to Heat-Induced Aggregation Despite Its Critical Role in Plant Heat Tolerance. Plant Physiol., 2018. 176(4): p. 3027-3045 [PMID:29444814]