PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Malpighiales; Euphorbiaceae; Crotonoideae; Manihoteae; Manihot

Family

WRKY

Protein Properties

Length: 599aa MW: 64977.2 Da PI: 6.6759

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Manes.01G189000.1.p	genome	JGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	95.7	3.1e-30	247	306	1	59
---SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 1 ldDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 ++Dg++WrKYGqK kg+++pr+YYrCt + +Cpv+k+v+r+aed +++++tYeg+Hnh+ Manes.01G189000.1.p 247 MNDGCQWRKYGQKIAKGNPCPRAYYRCTVSpTCPVRKQVQRCAEDMSILITTYEGTHNHP 306 58************************999**************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	1.0E-31	232	308	IPR003657	WRKY domain
SuperFamily	SSF118290	3.01E-27	240	308	IPR003657	WRKY domain
PROSITE profile	PS50811	28.575	242	308	IPR003657	WRKY domain
SMART	SM00774	2.1E-36	247	307	IPR003657	WRKY domain
Pfam	PF03106	9.5E-27	248	306	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 599 aa Download sequence Send to blast
MTMVDMRSSI PMATSLEKPD AVLQEEKKVM KSNGDEDGIL LGTTKGVDVM VHVKQEEKSR 60 KRPSSMDDQL RSAKAEIGEV REENERLKQL LSKMLKDYQS LEKHFREVVQ EEEANKSTKI 120 TPIYQENEES ELVSLSLGRS SSTEPKKEEK KSSNLTDENE DNEELNNKGL SLGLDCKFEL 180 DSSVTMKNPS SENSFDDEEA KEEEATETWP PSKMLKTTIS AGDEVLQQTQ TKKTRVSVRA 240 RCDTPTMNDG CQWRKYGQKI AKGNPCPRAY YRCTVSPTCP VRKQVQRCAE DMSILITTYE 300 GTHNHPLPLS ATAMASTTSA AASMLQSRSS SSQPGLGTSV SAPASMSTAN GLNFTVSQNN 360 AIRPHQIYFP NSSSSASTSH PTITLDLTAP TSTSHFNRFP MRYSATCLNF SSSASSTSLE 420 PNSALQTLWN PGYSTYDTLS HSRNNIIGSL NNIGKQPPQD HLYQPQMQMN RQTAATSQQS 480 LTETIAAATK VIALDPNFRS ALAAAITTFV GNGGSAVREN PGGGEHSAGL NSKLGDSLTF 540 NTLYPSSNGG IGCASSYLNK SPPTQQQGSL VSFPPPFPFS ASKSASGSPA SSRDYNKG*

Sequence ? help Back to Top

Protein Sequence Length: 599 aa Download sequence Send to blast

MTMVDMRSSI PMATSLEKPD AVLQEEKKVM KSNGDEDGIL LGTTKGVDVM VHVKQEEKSR  60
KRPSSMDDQL RSAKAEIGEV REENERLKQL LSKMLKDYQS LEKHFREVVQ EEEANKSTKI  120
TPIYQENEES ELVSLSLGRS SSTEPKKEEK KSSNLTDENE DNEELNNKGL SLGLDCKFEL  180
DSSVTMKNPS SENSFDDEEA KEEEATETWP PSKMLKTTIS AGDEVLQQTQ TKKTRVSVRA  240
RCDTPTMNDG CQWRKYGQKI AKGNPCPRAY YRCTVSPTCP VRKQVQRCAE DMSILITTYE  300
GTHNHPLPLS ATAMASTTSA AASMLQSRSS SSQPGLGTSV SAPASMSTAN GLNFTVSQNN  360
AIRPHQIYFP NSSSSASTSH PTITLDLTAP TSTSHFNRFP MRYSATCLNF SSSASSTSLE  420
PNSALQTLWN PGYSTYDTLS HSRNNIIGSL NNIGKQPPQD HLYQPQMQMN RQTAATSQQS  480
LTETIAAATK VIALDPNFRS ALAAAITTFV GNGGSAVREN PGGGEHSAGL NSKLGDSLTF  540
NTLYPSSNGG IGCASSYLNK SPPTQQQGSL VSFPPPFPFS ASKSASGSPA SSRDYNKG*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
2ayd_A	6e-21	234	308	1	74	WRKY transcription factor 1
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription activator involved in the transcriptional regulation of terpene biosynthesis in glandular trichomes (PubMed:24884371). Binds to the promoter of the linalool synthase TPS5 and promotes TPS5 gene transactivation (PubMed:24884371). In association with WRKY72B, contributes to basal defense against root-knot nematodes (RKNs) and potato aphids, as well as Mi-1-mediated gene-for-gene resistance to these pests (PubMed:20409007). Both WRKY72A and WRKY72B are not required for gene-for-gene resistance mediated by Pto, another tomato R gene (PubMed:20409007). {ECO:0000269\|PubMed:20409007, ECO:0000269\|PubMed:24884371}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Manes.01G189000.1.p

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Induced by infection with the root-knot nematode (RKN) Meloidogyne incognita and potato aphids. {ECO:0000269\|PubMed:20409007}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	KT827613	0.0	KT827613.1 Manihot esculenta WRKY transcription factor 38 mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_021621556.1	0.0	probable WRKY transcription factor 72
Swissprot	A0A3Q7GFB5	1e-115	WK72A_SOLLC; WRKY transcription factor 72A
TrEMBL	A0A2C9WM20	0.0	A0A2C9WM20_MANES; Uncharacterized protein
STRING	cassava4.1_026701m	0.0	(Manihot esculenta)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Fabids	OGEF650	34	134

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT5G15130.1	1e-72	WRKY DNA-binding protein 72

Link Out ? help Back to Top
Phytozome	Manes.01G189000.1.p

Publications ? help Back to Top
Tomato Genome Consortium The tomato genome sequence provides insights into fleshy fruit evolution. Nature, 2012. 485(7400): p. 635-41 [PMID:22660326] Spyropoulou EA,Haring MA,Schuurink RC RNA sequencing on Solanum lycopersicum trichomes identifies transcription factors that activate terpene synthase promoters. BMC Genomics, 2014. 15: p. 402 [PMID:24884371]