PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum

Family

WRKY

Protein Properties

Length: 368aa MW: 39910.1 Da PI: 5.6089

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
MLOC_74884.1	genome	IBSC	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	100.5	1e-31	189	246	2	60
--SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS-- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhek 60 dDgynWrKYGqK+vkgse+prsYY+Ct+++C+vkk +er+ d+ ++e++Y+g+Hnh+k MLOC_74884.1 189 DDGYNWRKYGQKHVKGSENPRSYYKCTHPNCEVKKLLERAV-DGLITEVVYKGRHNHPK 246 8**************************************.*************85 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	8.6E-28	181	247	IPR003657	WRKY domain
PROSITE profile	PS50811	23.525	183	247	IPR003657	WRKY domain
SuperFamily	SSF118290	1.1E-24	187	247	IPR003657	WRKY domain
SMART	SM00774	7.1E-35	188	246	IPR003657	WRKY domain
Pfam	PF03106	2.3E-24	189	245	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0005634	Cellular Component	nucleus
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 368 aa Download sequence Send to blast
MSPARLPISR ESCLTIPAGF SPSALLDSPV LLTNFKVEPS PTTGSLGMAA ILHKSAHPDM 60 LPSPRDKSVR NAHEDRGSRD FEFKPHLNSS SQSLAPAMSD LKKHEHSMQN QSMNPSSSSS 120 NMVNENRPPC SRESSLTVNV SAQNQPVGMV GLTDSMPAEV GTSEPQQMNS SDNAMQEPQS 180 ENVADKSADD GYNWRKYGQK HVKGSENPRS YYKCTHPNCE VKKLLERAVD GLITEVVYKG 240 RHNHPKPQPN RRLAGGAVPS NQGEERYDGA SAADDKSSNA LSNLANPVHS PGMVEPVPAS 300 VSDDDIDAGG GRPYPGDDAT EEEDLESKRR KMESAGIDAA LMGKPNREPR VVVQTVSEVD 360 ILDDGYRW

Sequence ? help Back to Top

Protein Sequence Length: 368 aa Download sequence Send to blast

MSPARLPISR ESCLTIPAGF SPSALLDSPV LLTNFKVEPS PTTGSLGMAA ILHKSAHPDM  60
LPSPRDKSVR NAHEDRGSRD FEFKPHLNSS SQSLAPAMSD LKKHEHSMQN QSMNPSSSSS  120
NMVNENRPPC SRESSLTVNV SAQNQPVGMV GLTDSMPAEV GTSEPQQMNS SDNAMQEPQS  180
ENVADKSADD GYNWRKYGQK HVKGSENPRS YYKCTHPNCE VKKLLERAVD GLITEVVYKG  240
RHNHPKPQPN RRLAGGAVPS NQGEERYDGA SAADDKSSNA LSNLANPVHS PGMVEPVPAS  300
VSDDDIDAGG GRPYPGDDAT EEEDLESKRR KMESAGIDAA LMGKPNREPR VVVQTVSEVD  360
ILDDGYRW

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1wj2_A	4e-16	189	247	18	77	Probable WRKY transcription factor 4
2lex_A	4e-16	189	247	18	77	Probable WRKY transcription factor 4
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor involved in starch synthesis (PubMed:12953112). Acts as a transcriptional activator in sugar signaling (PubMed:16167901). Interacts specifically with the SURE and W-box elements, but not with the SP8a element (PubMed:12953112). {ECO:0000269\|PubMed:12953112, ECO:0000269\|PubMed:16167901}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	MLOC_74884.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Up-regulated by sugar. {ECO:0000269\|PubMed:12953112}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AY323206	0.0	AY323206.1 Hordeum vulgare SUSIBA2 (WRKY46) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_020176501.1	0.0	WRKY transcription factor SUSIBA2
Swissprot	Q6VWJ6	0.0	WRK46_HORVU; WRKY transcription factor SUSIBA2
TrEMBL	A0A287HPM5	0.0	A0A287HPM5_HORVV; Uncharacterized protein
STRING	MLOC_74884.1	0.0	(Hordeum vulgare)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Monocots	OGMP6740	38	51

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT4G26640.2	1e-79	WRKY family protein

Publications ? help Back to Top
Sun C, et al. A novel WRKY transcription factor, SUSIBA2, participates in sugar signaling in barley by binding to the sugar-responsive elements of the iso1 promoter. Plant Cell, 2003. 15(9): p. 2076-92 [PMID:12953112] Sun C,H Antisense oligodeoxynucleotide inhibition as a potent strategy in plant biology: identification of SUSIBA2 as a transcriptional activator in plant sugar signalling. Plant J., 2005. 44(1): p. 128-38 [PMID:16167901] Su J, et al. Expression of barley SUSIBA2 transcription factor yields high-starch low-methane rice. Nature, 2015. 523(7562): p. 602-6 [PMID:26200336]