PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum

Family

GRAS

Protein Properties

Length: 502aa MW: 53186.9 Da PI: 5.0394

Description

GRAS family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
MLOC_73617.2	genome	IBSC	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	GRAS	428.6	4.9e-131	87	449	1	374
GRAS 1 lvelLlecAeavssgdlelaqalLarlsel..aspdgdpmqRlaayfteALaarlarsvselykalppsetseknsseelaalklfsevsPilkfsh 95 lv+lL++cA av++gd++la a La + a ++++ + R+a +ft AL++rl+r + +p + + +e+++ ++ f+e++P+lkf+h MLOC_73617.2 87 LVHLLMSCAGAVEAGDHALAAAHLADANASlaALSTASGIGRVALHFTDALSRRLFR------SPTTPPP----TDAEHAFLYHHFYEACPYLKFAH 173 68**********************999874556669***************......2222222....357999999********** PP GRAS 96 ltaNqaIleavegeervHiiDfdisqGlQWpaLlqaLasRpegppslRiTgvgspesgskeeleetgerLakfAeelgvpfefnvlvakrledlele 192 +taNqaIlea++g+++vH+iDf+++qGlQWpaL+qaLa Rp+gpp lRiTg+g+p++ ++el+ +g rLa++A++++v+f+f+ ++a++l++++++ MLOC_73617.2 174 FTANQAILEAFHGCDTVHVIDFSLMQGLQWPALIQALALRPGGPPFLRITGIGPPSPPGRDELRDVGLRLADLARSVRVRFSFRGVAANSLDEVHPW 270 ********************************************************************************************* PP GRAS 193 eLrvkpgEalaVnlvlqlhrlldesvsleserdevLklvkslsPkvvvvveqeadhnsesFlerflealeyysalfdsleaklpreseerikvErel 289 +L++ pgEa+aVn+vlqlhrll++s+++ +d+vL++v sl+Pk+++vveqe+dhn++ Fl+rf+eal yysa+fdsl+a +++ + + + +++ MLOC_73617.2 271 MLQIAPGEAVAVNSVLQLHRLLADSADQVP-IDAVLDCVASLQPKIFTVVEQEVDHNKPGFLDRFTEALFYYSAVFDSLDAA--SANGTGNAMAEAY 364 ******************99999998.********************************************988..6889999999999 PP GRAS 290 lgreivnvvacegaerrerhetlekWrerleeaGFkpvplsekaakqaklllrkvksdgyrveeesgslvlgWkdrpLvsvSaWr 374 l+rei+++v++eg++r erhe l++Wr+rl++aG+++vpl+ +a qa++ll ++++g++vee +g+l+lgW++rpL+s+SaWr MLOC_73617.2 365 LQREICDIVCNEGTARMERHEPLSQWRDRLGRAGLTAVPLGASALRQARMLLGLFSGEGHSVEEAEGCLTLGWHGRPLFSASAWR 449 **********************************************************************************8 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS50985	62.287	61	429	IPR005202	Transcription factor GRAS
Pfam	PF03514	1.7E-128	87	449	IPR005202	Transcription factor GRAS

Sequence ? help Back to Top
Protein Sequence Length: 502 aa Download sequence Send to blast
MAMGTFPFQW PMDPAPAPTS SLDASLPPSL LPPPPAAVPD DGAAYYAAAD MHASSMPDLA 60 PPFPSRDAVA AELAMRRAEE EVAGIRLVHL LMSCAGAVEA GDHALAAAHL ADANASLAAL 120 STASGIGRVA LHFTDALSRR LFRSPTTPPP TDAEHAFLYH HFYEACPYLK FAHFTANQAI 180 LEAFHGCDTV HVIDFSLMQG LQWPALIQAL ALRPGGPPFL RITGIGPPSP PGRDELRDVG 240 LRLADLARSV RVRFSFRGVA ANSLDEVHPW MLQIAPGEAV AVNSVLQLHR LLADSADQVP 300 IDAVLDCVAS LQPKIFTVVE QEVDHNKPGF LDRFTEALFY YSAVFDSLDA ASANGTGNAM 360 AEAYLQREIC DIVCNEGTAR MERHEPLSQW RDRLGRAGLT AVPLGASALR QARMLLGLFS 420 GEGHSVEEAE GCLTLGWHGR PLFSASAWRA AAVGCDADGE DNHTSNNTSN FSGSSGGGSD 480 SNNSSSSNGK CSGVVGASSM FL

Sequence ? help Back to Top

Protein Sequence Length: 502 aa Download sequence Send to blast

MAMGTFPFQW PMDPAPAPTS SLDASLPPSL LPPPPAAVPD DGAAYYAAAD MHASSMPDLA  60
PPFPSRDAVA AELAMRRAEE EVAGIRLVHL LMSCAGAVEA GDHALAAAHL ADANASLAAL  120
STASGIGRVA LHFTDALSRR LFRSPTTPPP TDAEHAFLYH HFYEACPYLK FAHFTANQAI  180
LEAFHGCDTV HVIDFSLMQG LQWPALIQAL ALRPGGPPFL RITGIGPPSP PGRDELRDVG  240
LRLADLARSV RVRFSFRGVA ANSLDEVHPW MLQIAPGEAV AVNSVLQLHR LLADSADQVP  300
IDAVLDCVAS LQPKIFTVVE QEVDHNKPGF LDRFTEALFY YSAVFDSLDA ASANGTGNAM  360
AEAYLQREIC DIVCNEGTAR MERHEPLSQW RDRLGRAGLT AVPLGASALR QARMLLGLFS  420
GEGHSVEEAE GCLTLGWHGR PLFSASAWRA AAVGCDADGE DNHTSNNTSN FSGSSGGGSD  480
SNNSSSSNGK CSGVVGASSM FL

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
5hyz_A	2e-61	87	449	4	375	GRAS family transcription factor containing protein, expressed
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Transcription coactivator of the zinc finger transcription factors GAF1/IDD2 and ENY/IDD1 in regulation of gibberellin homeostasis and signaling (PubMed:25035403). No effect of the BOI proteins on its stability. Probably acts by participating in large multiprotein complexes that repress transcription of GA-inducible genes. Positively regulates XERICO expression. In contrast to RGA, it is less sensitive to GA. Its activity is probably regulated by other phytohormones such as auxin and ethylene. {ECO:0000269\|PubMed:11487693, ECO:0000269\|PubMed:11606551, ECO:0000269\|PubMed:11606552, ECO:0000269\|PubMed:14973286, ECO:0000269\|PubMed:15128937, ECO:0000269\|PubMed:16034591, ECO:0000269\|PubMed:17933900, ECO:0000269\|PubMed:25035403, ECO:0000269\|PubMed:9389651}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	MLOC_73617.2

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AK362142	0.0	AK362142.1 Hordeum vulgare subsp. vulgare mRNA for predicted protein, complete cds, clone: NIASHv2002N03.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_020192227.1	0.0	DELLA protein SLN1-like
Swissprot	Q9LQT8	1e-131	GAI_ARATH; DELLA protein GAI
TrEMBL	A0A287L6Q8	0.0	A0A287L6Q8_HORVV; Uncharacterized protein
STRING	MLOC_73617.2	0.0	(Hordeum vulgare)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Monocots	OGMP998	38	138

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT1G14920.1	1e-121	GRAS family protein

Publications ? help Back to Top
Duarte JM, et al. Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. Mol. Biol. Evol., 2006. 23(2): p. 469-78 [PMID:16280546] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Gallego-Giraldo C, et al. Role of the gibberellin receptors GID1 during fruit-set in Arabidopsis. Plant J., 2014. 79(6): p. 1020-1032 [PMID:24961590] Fukazawa J, et al. DELLAs function as coactivators of GAI-ASSOCIATED FACTOR1 in regulation of gibberellin homeostasis and signaling in Arabidopsis. Plant Cell, 2014. 26(7): p. 2920-38 [PMID:25035403] Marín-de la Rosa N, et al. Genome Wide Binding Site Analysis Reveals Transcriptional Coactivation of Cytokinin-Responsive Genes by DELLA Proteins. PLoS Genet., 2015. 11(7): p. e1005337 [PMID:26134422] Shi H,Wei Y,Wang Q,Reiter RJ,He C Melatonin mediates the stabilization of DELLA proteins to repress the floral transition in Arabidopsis. J. Pineal Res., 2016. 60(3): p. 373-9 [PMID:26887824] Lee SA, et al. Interplay between ABA and GA Modulates the Timing of Asymmetric Cell Divisions in the Arabidopsis Root Ground Tissue. Mol Plant, 2016. 9(6): p. 870-84 [PMID:26970019] Qu J,Kang SG,Hah C,Jang JC Molecular and cellular characterization of GA-Stimulated Transcripts GASA4 and GASA6 in Arabidopsis thaliana. Plant Sci., 2016. 246: p. 1-10 [PMID:26993231] Shahnejat-Bushehri S,Nobmann B,Devi Allu A,Balazadeh S JUB1 suppresses Pseudomonas syringae-induced defense responses through accumulation of DELLA proteins. Plant Signal Behav, 2016. 11(6): p. e1181245 [PMID:27159137] Shahnejat-Bushehri S,Tarkowska D,Sakuraba Y,Balazadeh S Arabidopsis NAC transcription factor JUB1 regulates GA/BR metabolism and signalling. Nat Plants, 2016. 2: p. 16013 [PMID:27249348] Wang H, et al. The DELLA-CONSTANS Transcription Factor Cascade Integrates Gibberellic Acid and Photoperiod Signaling to Regulate Flowering. Plant Physiol., 2016. 172(1): p. 479-88 [PMID:27406167] Li W,Wang H,Yu D Arabidopsis WRKY Transcription Factors WRKY12 and WRKY13 Oppositely Regulate Flowering under Short-Day Conditions. Mol Plant, 2016. 9(11): p. 1492-1503 [PMID:27592586] Liu B,De Storme N,Geelen D Gibberellin Induces Diploid Pollen Formation by Interfering with Meiotic Cytokinesis. Plant Physiol., 2017. 173(1): p. 338-353 [PMID:27621423] Matsuoka K, et al. Differential Cellular Control by Cotyledon-Derived Phytohormones Involved in Graft Reunion of Arabidopsis Hypocotyls. Plant Cell Physiol., 2016. 57(12): p. 2620-2631 [PMID:27986917] Zentella R, et al. The Arabidopsis O-fucosyltransferase SPINDLY activates nuclear growth repressor DELLA. Nat. Chem. Biol., 2017. 13(5): p. 479-485 [PMID:28244988] Zhang Y, et al. GA-DELLA pathway is involved in regulation of nitrogen deficiency-induced anthocyanin accumulation. Plant Cell Rep., 2017. 36(4): p. 557-569 [PMID:28275852] Zhang L,Chen L,Yu D Transcription Factor WRKY75 Interacts with DELLA Proteins to Affect Flowering. Plant Physiol., 2018. 176(1): p. 790-803 [PMID:29133369] Nelson SK,Ariizumi T,Steber CM Biology in the Dry Seed: Transcriptome Changes Associated with Dry Seed Dormancy and Dormancy Loss in the Arabidopsis GA-Insensitive sleepy1-2 Mutant. Front Plant Sci, 2017. 8: p. 2158 [PMID:29312402] Liu B,De Storme N,Geelen D Cold-Induced Male Meiotic Restitution in Arabidopsis thaliana Is Not Mediated by GA-DELLA Signaling. Front Plant Sci, 2018. 9: p. 91 [PMID:29459879] Zhang Y, et al. DELLA proteins negatively regulate dark-induced senescence and chlorophyll degradation in Arabidopsis through interaction with the transcription factor WRKY6. Plant Cell Rep., 2018. 37(7): p. 981-992 [PMID:29574486] Felipo-Benavent A, et al. Regulation of xylem fiber differentiation by gibberellins through DELLA-KNAT1 interaction. Development, 2019. [PMID:30389856] Wright DA, et al. Recovery of YAC-end sequences through complementation of an Escherichia coli pyrF mutation. Nucleic Acids Res., 1997. 25(13): p. 2679-80 [PMID:9185581]