PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum

Family

WRKY

Protein Properties

Length: 489aa MW: 52849.9 Da PI: 6.0591

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
MLOC_5850.1	genome	IBSC	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	87.2	1.4e-27	218	273	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhe 59 dDgynWrKYGqK vkg ++prsYY+Ct +Cpv+k+ve+sa d+++++i+Y+g+H he MLOC_5850.1 218 DDGYNWRKYGQKAVKGGKCPRSYYKCTL-NCPVRKNVEHSA-DGRIIKIVYRGQHCHE 273 8***********************97.7*******.*************7 PP
2	WRKY	102.4	2.5e-32	383	441	1	59
---SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 1 ldDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhe 59 ldDgy+WrKYGqK+v+g+++prsYY+Ct++gC vkk+vers+++p++v++tYeg+H+h+ MLOC_5850.1 383 LDDGYRWRKYGQKVVRGNPHPRSYYKCTYQGCDVKKHVERSSQEPHAVITTYEGKHTHD 441 59********************************************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	4.2E-25	214	275	IPR003657	WRKY domain
SuperFamily	SSF118290	1.06E-21	215	275	IPR003657	WRKY domain
SMART	SM00774	1.7E-29	217	274	IPR003657	WRKY domain
Pfam	PF03106	4.3E-22	218	272	IPR003657	WRKY domain
PROSITE profile	PS50811	20.954	218	275	IPR003657	WRKY domain
Gene3D	G3DSA:2.20.25.80	6.3E-35	370	443	IPR003657	WRKY domain
SuperFamily	SSF118290	4.84E-29	376	443	IPR003657	WRKY domain
PROSITE profile	PS50811	35.909	378	443	IPR003657	WRKY domain
SMART	SM00774	3.3E-38	383	442	IPR003657	WRKY domain
Pfam	PF03106	3.4E-25	384	441	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 489 aa Download sequence Send to blast
MAAGQWSAIG DGGGLWSPQA LESLFPDEQP AAAQLGFFGG SLAQLAPPPP LCAIALLGYP 60 QDNFDVFNEQ DLAQVAAQVA QKKELQEKQA ALLHPKVAPQ LAFPKYSILD RVDNSSSFKP 120 ATSVLTPQHV TSVIAASMQG MSTLPSHTGS GSMNTGPTGI LQVLQGSSTT LDSINTGSAG 180 VLQVLQGSST TLDSINTGSA GFLEALQGSS ITLDKPADDG YNWRKYGQKA VKGGKCPRSY 240 YKCTLNCPVR KNVEHSADGR IIKIVYRGQH CHEPPSKRFK DCGDLLNELD ELNDAEEPST 300 RSLLGCQGYY GKPKPITPNG TMVDGLLPTK EEGDEQLSSL SDIREDDGEI RTVDGDVGDA 360 DANERNAPGQ KIIVSTTSDV DLLDDGYRWR KYGQKVVRGN PHPRSYYKCT YQGCDVKKHV 420 ERSSQEPHAV ITTYEGKHTH DVPESRNRSQ ATGSHHCKEQ TYSEQPAASF CSSSEKRKYG 480 TVILNDLAF

Sequence ? help Back to Top

Protein Sequence Length: 489 aa Download sequence Send to blast

MAAGQWSAIG DGGGLWSPQA LESLFPDEQP AAAQLGFFGG SLAQLAPPPP LCAIALLGYP  60
QDNFDVFNEQ DLAQVAAQVA QKKELQEKQA ALLHPKVAPQ LAFPKYSILD RVDNSSSFKP  120
ATSVLTPQHV TSVIAASMQG MSTLPSHTGS GSMNTGPTGI LQVLQGSSTT LDSINTGSAG  180
VLQVLQGSST TLDSINTGSA GFLEALQGSS ITLDKPADDG YNWRKYGQKA VKGGKCPRSY  240
YKCTLNCPVR KNVEHSADGR IIKIVYRGQH CHEPPSKRFK DCGDLLNELD ELNDAEEPST  300
RSLLGCQGYY GKPKPITPNG TMVDGLLPTK EEGDEQLSSL SDIREDDGEI RTVDGDVGDA  360
DANERNAPGQ KIIVSTTSDV DLLDDGYRWR KYGQKVVRGN PHPRSYYKCT YQGCDVKKHV  420
ERSSQEPHAV ITTYEGKHTH DVPESRNRSQ ATGSHHCKEQ TYSEQPAASF CSSSEKRKYG  480
TVILNDLAF

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1wj2_A	2e-33	218	443	8	78	Probable WRKY transcription factor 4
2lex_A	2e-33	218	443	8	78	Probable WRKY transcription factor 4
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). {ECO:0000250}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	MLOC_5850.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By salicylic acid and during leaf senescence. {ECO:0000269\|PubMed:11449049, ECO:0000269\|PubMed:11722756}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AK365469	0.0	AK365469.1 Hordeum vulgare subsp. vulgare mRNA for predicted protein, complete cds, clone: NIASHv2034G15.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_020146250.1	0.0	probable WRKY transcription factor 3
Swissprot	Q9ZQ70	3e-67	WRKY3_ARATH; Probable WRKY transcription factor 3
TrEMBL	A0A287HNH7	0.0	A0A287HNH7_HORVV; Uncharacterized protein
STRING	MLOC_5850.1	0.0	(Hordeum vulgare)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Monocots	OGMP1345	38	122

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT2G03340.1	2e-66	WRKY DNA-binding protein 3

Publications ? help Back to Top
Duarte JM, et al. Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. Mol. Biol. Evol., 2006. 23(2): p. 469-78 [PMID:16280546] Aamir M, et al. Structural and Functional Insights into WRKY3 and WRKY4 Transcription Factors to Unravel the WRKY-DNA (W-Box) Complex Interaction in Tomato (Solanum lycopersicum L.). A Computational Approach. Front Plant Sci, 2017. 8: p. 819 [PMID:28611792]