PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Rosales; Rosaceae; Maloideae; Maleae; Malus

Family

WRKY

Protein Properties

Length: 734aa MW: 79578.9 Da PI: 6.3863

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
MDP0000431358	genome	GDR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	101.7	4.2e-32	313	370	2	60
--SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS-- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhek 60 +DgynWrKYGqK+vkgse+prsYY+Ct+++C+vkkkvers+ +++v+ei+Y+g Hnh+k MDP0000431358 313 EDGYNWRKYGQKQVKGSEYPRSYYKCTHPNCQVKKKVERSQ-EGHVTEIIYKGAHNHPK 370 7**************************************.*************85 PP
2	WRKY	102.8	2e-32	524	582	1	59
---SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 1 ldDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhe 59 ldDgy+WrKYGqK+vkg+++prsYY+Ct agC+v+k+ver+++d k v++tYeg+Hnh+ MDP0000431358 524 LDDGYRWRKYGQKVVKGNPNPRSYYKCTNAGCTVRKHVERASHDLKSVITTYEGKHNHD 582 59********************************************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS50811	23.204	307	371	IPR003657	WRKY domain
SuperFamily	SSF118290	4.05E-25	312	371	IPR003657	WRKY domain
SMART	SM00774	8.3E-36	312	370	IPR003657	WRKY domain
Gene3D	G3DSA:2.20.25.80	1.3E-27	312	371	IPR003657	WRKY domain
Pfam	PF03106	1.1E-24	313	369	IPR003657	WRKY domain
Gene3D	G3DSA:2.20.25.80	9.3E-37	509	584	IPR003657	WRKY domain
SuperFamily	SSF118290	1.96E-28	516	584	IPR003657	WRKY domain
PROSITE profile	PS50811	38.32	519	584	IPR003657	WRKY domain
SMART	SM00774	1.9E-37	524	583	IPR003657	WRKY domain
Pfam	PF03106	7.5E-25	525	582	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009555	Biological Process	pollen development
GO:0009942	Biological Process	longitudinal axis specification
GO:0030010	Biological Process	establishment of cell polarity
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 734 aa Download sequence Send to blast
MAGIDDNIAI IGDWVPPSPS PRAFFSSMLV DDIGSRSILE PHXNNRTEDF FLGAREGDNN 60 GKNWSQGNVS GEDITEVGSF PEYKSNSRGG LVERIAARAG FNAPRLNTES IRSSDLSHTS 120 DIRSPYLTIP PGLSPTTLLD SPVFLSNSLA QPSPTTGKFP FASNGHGRSS TLMPESPDKN 180 NFFEDINASF AFKPVAESGS FFLGLTGKMG SGTFQQQSFP SMEVSVQSEN SSQSIEPTKV 240 QXQNTNNLQH QADFSHTSTE KDNGAYTISA DPRAFDTVGG STEHSPPLDE QPDEERGSGD 300 SMAAGGGGGA ASEDGYNWRK YGQKQVKGSE YPRSYYKCTH PNCQVKKKVE RSQEGHVTEI 360 IYKGAHNHPK PPPNRRSAAM GSSNPLVDMR TDIPEQGGAD GDSIWANTQK GNVGGPDWKH 420 DNLEVTSSAS VGPDYCNQSS MQAQNGTHHK SGDVVDASST FSNDEEEDDR GTHGSVSLAY 480 DGEGDESESK RRKIEAYATE MSGATRAIRE PRVVVQTTSE VDILDDGYRW RKYGQKVVKG 540 NPNPRSYYKC TNAGCTVRKH VERASHDLKS VITTYEGKHN HDVPAARNSS HVNSGPSSNM 600 PGQASTIQTH PHRPEPQVHN GMGRFERPSS MGSFNLPGRQ QMGPSHAFSF GMNQPGFANL 660 SMAGLGPGQP KLSVLPSHHP YFAQQRHQVN EMAFMLPKGE PKVEPMSEPG LIMSNGSSVY 720 QQLMSRLPLG PQM*

Sequence ? help Back to Top

Protein Sequence Length: 734 aa Download sequence Send to blast

MAGIDDNIAI IGDWVPPSPS PRAFFSSMLV DDIGSRSILE PHXNNRTEDF FLGAREGDNN  60
GKNWSQGNVS GEDITEVGSF PEYKSNSRGG LVERIAARAG FNAPRLNTES IRSSDLSHTS  120
DIRSPYLTIP PGLSPTTLLD SPVFLSNSLA QPSPTTGKFP FASNGHGRSS TLMPESPDKN  180
NFFEDINASF AFKPVAESGS FFLGLTGKMG SGTFQQQSFP SMEVSVQSEN SSQSIEPTKV  240
QXQNTNNLQH QADFSHTSTE KDNGAYTISA DPRAFDTVGG STEHSPPLDE QPDEERGSGD  300
SMAAGGGGGA ASEDGYNWRK YGQKQVKGSE YPRSYYKCTH PNCQVKKKVE RSQEGHVTEI  360
IYKGAHNHPK PPPNRRSAAM GSSNPLVDMR TDIPEQGGAD GDSIWANTQK GNVGGPDWKH  420
DNLEVTSSAS VGPDYCNQSS MQAQNGTHHK SGDVVDASST FSNDEEEDDR GTHGSVSLAY  480
DGEGDESESK RRKIEAYATE MSGATRAIRE PRVVVQTTSE VDILDDGYRW RKYGQKVVKG  540
NPNPRSYYKC TNAGCTVRKH VERASHDLKS VITTYEGKHN HDVPAARNSS HVNSGPSSNM  600
PGQASTIQTH PHRPEPQVHN GMGRFERPSS MGSFNLPGRQ QMGPSHAFSF GMNQPGFANL  660
SMAGLGPGQP KLSVLPSHHP YFAQQRHQVN EMAFMLPKGE PKVEPMSEPG LIMSNGSSVY  720
QQLMSRLPLG PQM*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1wj2_A	2e-36	313	585	8	78	Probable WRKY transcription factor 4
2lex_A	2e-36	313	585	8	78	Probable WRKY transcription factor 4
Search in ModeBase

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
Mdo.16894	0.0	fruit

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Low expression in senescent leaves (PubMed:11722756). Expressed in both the unfertilized egg cell and the pollen tube (PubMed:21316593). {ECO:0000269\|PubMed:11722756, ECO:0000269\|PubMed:21316593}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Regulates WOX8 and WOX9 expression and basal cell division patterns during early embryogenesis. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Required to repolarize the zygote from a transient symmetric state. {ECO:0000269\|PubMed:21316593}.

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00071	PBM	Transfer from AT5G56270	Download

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	KJ950367	0.0	KJ950367.1 Pyrus x bretschneideri WRKY domain class transcription factor (WRKY7) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_008344758.2	0.0	probable WRKY transcription factor 2 isoform X1
Refseq	XP_008344759.2	0.0	probable WRKY transcription factor 2 isoform X1
Refseq	XP_008344760.2	0.0	probable WRKY transcription factor 2 isoform X1
Refseq	XP_017180259.2	0.0	probable WRKY transcription factor 2 isoform X1
Swissprot	Q9FG77	0.0	WRKY2_ARATH; Probable WRKY transcription factor 2
TrEMBL	A0A498K9B0	0.0	A0A498K9B0_MALDO; Uncharacterized protein
STRING	XP_008344758.1	0.0	(Malus domestica)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Fabids	OGEF9524	34	43

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT5G56270.1	0.0	WRKY DNA-binding protein 2

Link Out ? help Back to Top
Phytozome	MDP0000431358

Publications ? help Back to Top
Duarte JM, et al. Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. Mol. Biol. Evol., 2006. 23(2): p. 469-78 [PMID:16280546] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Ueda M, et al. Transcriptional integration of paternal and maternal factors in the Arabidopsis zygote. Genes Dev., 2017. 31(6): p. 617-627 [PMID:28404632] Lei R, et al. Arabidopsis WRKY2 and WRKY34 transcription factors interact with VQ20 protein to modulate pollen development and function. Plant J., 2017. 91(6): p. 962-976 [PMID:28635025]